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A conserved region in the 3' untranslated region of the human LIMK1 gene is critical for proper expression of LIMK1 at the post-transcriptional level

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Neuroscience Bulletin 2013年第3期29卷 348-354页

作者： Guang-Fei Deng Shu-Jing Liu xun-sha sun Wei-Wen sun Qi-Hua Zhao Wei-Ping Liao Yong-Hong Yi Yue-Sheng Long Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and the Ministry of Education of China Institute of Neuroscience and the Second Affiliated Hospital of Guangzhou Medical University Key Laboratory for Major Obstetric Diseases of Guangdong Province and Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes Guangzhou Medical University

LIM kinase 1 （LIMK1）, a cytosolic serine/threonine kinase, regulates actin filament dynamics and reorganization and is involved in neuronal development and brain function. Abnormal expression of LIMK1 is associated with several neurological disorders. In this study, we performed a conservation analysis using Vector NTI （8.0） software. The dualluciferase reporter assay and real-time quantitative RT-PCR were used to assess the protein and mRNA levels of the reporter gene, respectively. We found that a region ranging from nt ＋884 to ＋966 in the human LIMK1 3＇ untranslated region （UTR） was highly conserved in the mouse Limkl 3＇ UTR and formed a structure containing several loops and stems. Luciferase assay showed that the relative luciferase activity of the mutated construct with the conserved region deleted, pGL4-hLIMK1-3U-M, in SH-SY5Y and HEK-293 cells was only -60% of that of the wild-type construct pGL4-hLIMK1-3U, indicating that the conserved region is critical for the reporter gene expression. Real-time quantitative RT-PCR analysis demonstrated that the relative Luc2 mRNA levels in SH-SY5Y and HEK293 cells transfected with pGL4-hLIMK1-3U-M decreased to50% of that in cells transfected with pGL4-hLIMK1- 3U, suggesting an important role of the conserved region in maintaining Luc2 mRNA stability. Our study suggests that the conserved region in the LIMK1 3＇ UTR is involved in regulating LIMK1 expression at the post-transcriptional level, which may help reveal the mechanism underlying the regulation of LIMK1 expression in the central nervous system and explore the relationship between the 3＇-UTR mutant and neuroloqical disorders.

关键词： LIM kinase 1 region post-transcriptsecondary structurethree-prime untranslatedonal regulation RNA

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Zoledronate-loaded aluminum salt nanovaccines amplify cellular immune response by enhancing cross-presentation

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Nano Research 2025年第1期18卷 559-571页

作者： Chunting He Penghui He Xue Tang Shuting Bai Ming Qin Yunting Zhang Zhaofei Guo Guangsheng Du xun sun Key Laboratory of Drug-Targeting and Drug Delivery System of the Education Ministry West China School of PharmacySichuan UniversityChengdu 610041China

Being a Th2 stimulator,classic aluminum salt-based adjuvants only stimulate weak cellular immune responses that are required for vaccination against intracellular viruses or cancerous *** a thirdgeneration bisphosphonate,zoledronate(ZOL)can enhance antigen crosspresentation by inhibiting key enzymes of the mevalonate ***,we developed the subunit antigen ovalbumin(OVA)and ZOL co-loaded aluminum hydroxide nanoparticles(APN-OVA-ZOL)and investigated their capacity for inducing cellular immune responses against the *** results showed that the developed nanovaccines could successfully encapsulate OVA and ZOL,and enabled efficient lymph node *** by the mevalonate pathway inhibition effect of ZOL,APN-OVA-ZOL significantly promoted *** a result,APN-OVA-ZOL induced robust cellular immunity,including the activation of T and B *** a EG7-OVA tumor-bearing murine model,APN-OVA-ZOL significantly inhibited the tumor growth and prolonged mice *** work provided a strong empirical foundation indicating that zoledronate-loaded aluminum salt nanovaccines had a strong potency for cancer immunotherapy.

关键词： zoledronate(ZOL) mevalonate pathway antigen cross-presentation cellular immune responses

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Cloning and characterization of the transcription regulatory region of human voltage-gated sodium channel SCN3A Gene

Cloning and characterization of the transcription regulatory...

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中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Yue-Sheng LONG,Jia-Ming QIN,xun-sha sun,Tao ZENG,Qi-Hua ZHAO,Wei-Ping LIAO~* Institute of Neuroscience and the Second Affiliated Hospital of Guangzhou Medical College Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and the Ministry of Education of China,Guangzhou 510260,China

＜正＞Voltage-gated sodium channels are responsible for the electrical excitability of the *** sodium channel genes are expressed broadly throughout the central nervous system（CNS）:SCN1 A,SCN2A,and *** in SCN1A and SCN2A are associated with several neurology disorders:epilepsy,familial hemiplegic migraine,and familial autism,and one mutation in SCN3A had been reported in a patient with cryptogenic pediatric partial *** in SCN3A transcript was found in animal seizure models,epileptic patients and peripheral nerve injury patients, which is believed to play a pivotal role in these *** date,the transcriptional mechanism of SCN3A is poorly *** identify the transcription start sites（TSS） for SCN3A,5’-rapid amplification of cDNA ends（5’-RACE） was carried out on human brain total RNA（Clontech） and the RACE products were sequenced by cloning into pGEM-T *** blast analysis of the 5’-RACE products with human genomic sequence was performed using BLAST software from NCBI *** characterize the functional promoter region and transcriptional regulatory elements,a 1.1- kb genomic fragment,5’deletion fragments and element-deletion fragments were cloned into the pGL4.10-basic expression vector upstream of the firefly luciferase cDNA and the recombinant constructs were transformed into cultured neuroblast cells and HEK-293 *** promoter activities were assessed by luciferase *** untranslated exon（228 bp, 197 bp and 50 bp respectively） were dispersed in 5’flanking region of human SCN3A and the TSS was located at 27.5 kb upstream of the first coding exon.A potential Initiator（INR）,an important element for general eukaryotic core promoter, was found at TSS.A mimic-TATA and a downstream regulatory element（DRE） were found at the -25 bp site and the +23 bp site,*** experiments demonstrated that the 1.1-kb genomic fragment was active in neuronal cell（SHSY5Y） and was inactive in non-

关键词： promoter transcription regulatory element sodium channel SCN3A

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Promoter analysis of mouse Scn3a gene and regulation of the promoter activity by GC box and CpG methylation

Promoter analysis of mouse Scn3a gene and regulation of the ...

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中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Guang-Fei DENG,Jia-Ming QIN,xun-sha sun,Zu-Ying KUANG,Tao SU,Qi-Hua ZHAO,Yi-Wu SHI,Xiao-Rong LIU,Mei-Juan YU,Yong-Hong YI,Wei-Ping LIAO,Yue-Sheng LONG Institute of Neuroscience and The Second Affiliated Hospital of Guangzhou Medical University

＜正＞Voltage-gated sodium channelα-subunit typeⅢ（Nav1.3） is mainly expressed in the central nervous system and is associated with neurological *** expression of mouse Scn3a product（Nav1.3） mainly occurs in embryonic and early postnatal brain but not in adult ***,we report for the first time the identification and characterization of the mouse Scn3a gene promoter region and regulation of the promoter activity by GC box and CpG *** assay showed that the promoter region F1.2（nt -1,049 to +157） had significantly higher activity in PC12 cells,omparing with that in SH-SY5Y cells and HEK293 cells.A stepwise 5’ truncation of the promoter region found that the minimal functional promoter located within the region nt -168 to +*** of a GC box（nt -254 to -258） in the mouse Scn3a promoter decreased the promoter *** methylation of the F1.2 without the GC box completely repressed the promoter activity,suggesting that the GC box is a critical element in the CpG-methylated Scn3a *** results suggest that the GC box and CpG methylation might play important roles in regulating mouse Scn3a gene expression.

关键词： voltage-gated sodium channel promoter GC box CpG methylation transcriptional regulation

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A Novel Receiving Method for Baseband DSSS Signal based on Phase Domain Processing

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China Communications 2010年第1期7卷 57-64页

作者： sha Xuejun Zhan Zongchao Tang xun Harbin Inst Technol Sch Elect & Informat Engn Harbin 150001 Heilongjiang Pr Peoples R China

In this paper, the phase characteristic disturbance model for baseband direct sequence spread spectrum (DSSS) signal in additive white Gaussian noise (AWGN) environment is established, and the probability density function (PDF) of phase characteristic disturbance is obtained. Then a novel receiver model for baseband DSSS signal based on maximum likelihood (ML) criterion is proposed. The simulation results show that, comparing with correlation scheme, the performance of the proposed method for baseband DSSS signal is 1dB worse in AWGN environment. However, if there is narrow interference in the AWGN environment, the proposed method will show better performance up to 2.5dB, and it has good adaptive resistance to narrowband interference located in different frequency points. This method could be used as an alterative communication scheme for military circumstance when existing strong narrowband interference of various frequencies.

关键词： phase domain processing phase characteristic disturbance ML criterion anti-narrow-band interference

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Recent advances in mRNA vaccine delivery

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Nano Research 2018年第10期11卷 5338-5354页

作者： Lu Tan xun sun Key Laboratory of Drug Targeting and Novel Drug Delivery System Ministry of Education West China School of Pharmacy SichuanUniversity Chengdu 61004 I China

In recent years, messenger RNA （mRNA） vaccines have been intensively studied in the fields of cancer immunotherapy and infectious diseases because of their excellent efficacy and safety profile. Despite significant progress in the rational design of mRNA vaccines and elucidation of their mechanism of action, their widespread application is limited by the development of safe and effective delivery systems that protect them from ubiquitous ribonucleases （RNases）, facilitate their entry into cells and subsequent escape from endosomes, and target them to lymphoid organs or particular cells. Some mRNA vaccines based on lipid carriers have entered clinical trials. Vaccines based on polymers, while not as clinically advanced as lipid vectors, show considerable potentials. In this review, we discuss the necessity of formulating mRNA vaccines with delivery systems, and we provide an overview of reported delivery systems.

关键词： messenger RNA (mRNA) vaccines delivery systems polymer lipid

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Observational study of land-atmosphere turbulent flux exchange over complex underlying surfaces in urban and suburban areas

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Science China Earth Sciences 2021年第7期64卷 1050-1064页

作者： Jie sha Jun ZOU Jianning sun School of Atmospheric Sciences Nanjing UniversityNanjing 210023China Joint International Research Laboratory of Atmospheric and Earth System Sciences Nanjing UniversityNanjing 210023China Jiangsu Provincial Collaborative Innovation Center of Climate Change Nanjing 210023China

Based on observation data from urban observation stations in Nanjing and Suzhou at two heights in the roughness sublayer above the canopy and observation data at three heights in the SORPES station at the Xianlin Campus of Nanjing University in a suburban area,the of land-atmosphere turbulent flux exchange and the energy balance over complex underlying surfaces were *** results indicated that in the roughness sublayer above the canopy,the nearsurface momentum flux,sensible heat flux,and latent heat flux increase with height,and the observation value of the surface albedo increases with ***,the observation value of the net radiation decreases with height,thus resulting in a change in the urban surface energy budget with *** the SORPES station in the Xianlin Campus of Nanjing University located in a hilly area,the momentum flux,sensible heat flux,and latent heat flux of the ground observation field significantly differed from those of the two heights on the tower,while the two heights on the tower were extremely *** results indicate that the flux observation over the complex underlying surface exhibits adequate local only when it is conducted at a higher altitude above the *** turbulent flux observation results at a lower altitude in urban areas are underestimated,while the turbulent flux observation results near the surface produce a large deviation over the underlying hilly complex.

关键词： Complex underlying surface Land-atmosphere exchange Turbulent flux Observation height Local representativeness

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A Hierarchical Forecasting Model for China's Foreign Trade

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Journal of Systems Science & Complexity 2020年第3期33卷 743-759页

作者： sun Yuying ZHANG xun WANG Shouyang Academy of Mathematics and Systems Science Chinese Academy of SciencesBeijing 100190China Center for Forecasting Science Chinese Academy of SciencesBeijing 100190China School of Economics and Management University of Chinese Academy of SciencesBeijing 100190China

Analyzing the underlying characteristics of trade values movements has attracted much attention in the domestic ***,the proposed understanding of these characteristics is limited by the intrinsic complexity of the imports/*** economic systems are naturally organized by hierarchies,a novel hierarchical model is proposed in this paper to forecast China's foreign ***,the foreign trade data are disaggregated from perspectives of trading partners and trading products,forming two independent hierarchical models with total exports and imports as target ***,a bottom-up strategy is *** bottom time series are modelled by corresponding control variables according to trading *** for bottom time series are then combined to generate initial forecasts for total exports and ***,forecasts for total imports and exports from the two hierarchical models,plus a single VECM model are combined to generate final *** experiments show that this proposed forecasting model approach significantly outperforms benchmark models and produces consistent forecasts for both total imports and exports or detailed items,which helps a lot for analyzing future trading structure evolution and making foreign trade policies.

关键词： Decomposed and combined models exports and imports forecasts hierarchy trade structure

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Insight into the development of electrolytes for aqueous zinc metal batteries from alkaline to neutral

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Chinese Chemical Letters 2024年第2期35卷 232-241页

作者： An Duan sha Luo Wei sun School of Materials and Energy University of Electronic Science and Technology of ChinaChengdu 611731China

Aqueous zinc metal batteries are considered as promising candidates for next-generation electrochemical energy storage devices,especially for large-scale energy storage,due to the advantages of high-safety,high energy density and low *** the bridge connecting cathode and anode,electrolyte provides a realistic operating *** alkaline and neutral aqueous zinc metal batteries,issues associated with electrolyte and anode are still *** this review,we reveal the development and evolution of electrolytes for aqueous zinc metal batteries from alkaline to neutral via the description of fundamentals and challenges in terms of comparison and *** also elaborate the strategies in electrolytes regulation and highlight the basic roles and progresses in additives engineering.

关键词： Aqueous electrolytes Zinc metal batteries pH-dependent chemistry Electrolyte regulation Additives engineering

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Turbulent Fluid Flow and Heat Transfer Calculation in Mold Filling and Solidification Processes of Castings

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Journal of Materials Science & Technology 2001年第1期17卷 69-70页

作者： Geying AN xun sun Junqing WANG National Key Laboratory of Precision Hot Processing of Metals Harbin institute of TechnologyHarbin 150001China Shenyang Research institute of Foundry Shenyang 110021China

Based on the time-averaging equations and a modified engineering turbulence model, the mold filling and solidification processes of castings are approximately described. The algorithm for the control equations is briefly introduced, and some problems and improvement methods for the traditional method are also presented. Both calculation and tests proved that. comparing with the laminar fluid flow and heat transfer, the simulation results by using the turbulence model are closer to the real mold filling and solidification processes of castings.

关键词： Solidification Processes Mold Filling solidification processes turbulence model fluid flow and heat transfer mold filling simulation results control equations methods for

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