Promoter analysis of mouse Scn3a gene and regulation of the promoter activity by GC box and CpG methylation
会议名称：《中国神经科学学会第九届全国学术会议暨第五次会员代表大会》
会议日期：2011年
学科分类：0710[理学-生物学] 07[理学] 071006[理学-神经生物学]
关 键 词：voltage-gated sodium channel promoter GC box CpG methylation transcriptional regulation
摘 要：正Voltage-gated sodium channelα-subunit typeⅢ（Nav1.3） is mainly expressed in the central nervous system and is associated with neurological *** expression of mouse Scn3a product（Nav1.3） mainly occurs in embryonic and early postnatal brain but not in adult ***,we report for the first time the identification and characterization of the mouse Scn3a gene promoter region and regulation of the promoter activity by GC box and CpG *** assay showed that the promoter region F1.2（nt -1,049 to +157） had significantly higher activity in PC12 cells,omparing with that in SH-SY5Y cells and HEK293 cells.A stepwise 5’ truncation of the promoter region found that the minimal functional promoter located within the region nt -168 to +*** of a GC box（nt -254 to -258） in the mouse Scn3a promoter decreased the promoter *** methylation of the F1.2 without the GC box completely repressed the promoter activity,suggesting that the GC box is a critical element in the CpG-methylated Scn3a *** results suggest that the GC box and CpG methylation might play important roles in regulating mouse Scn3a gene expression.