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Low-noise fluorescent detection of cardiac troponin I in human serum based on surface acoustic wave separation

Microsystems & Nanoengineering 2023年第6期9卷 261-272页

作者： Xuan Chen Chuanyu Zhang Xianglian Liu Yangchao Dong Hao Meng Xianming Qin Zhuangde Jiang xueyong wei State Key Laboratory for Manufacturing Systems Engineering Xi'an Jiaotong UniversityXian 710049China Department of Microbiology School of Preclinical MedicineFourth Military Medical UniversityXi'an 710032China The Second Affliated Hospital of Xi'an Jiaotong University Xi'an 710004China School of Mechano-Electronic Engineering Xidian UniversityXi'an 710071China

Acute myocardial infarction(AMI)is a life-threatening disease when sudden blockage of coronary artery occurs.As the most specific biomarker,cardiac troponin I(cTnI)is usually checked separately to diagnose or eliminate AMI,and achieving the accurate detection of cTnI is of great significance to patients'life and health.Compared with other methods,fluorescent detection has the advantages of simple operation,high sensitivity and wide applicability.However,due to the strong fluorescence interference of biological molecules in body fluids,it is often difficult to obtain high sensitivity.In order to solve this problem,in this study,surface acoustic wave separation is designed to purify the target to achieve more sensitive detection performance of fluorescent detection.Specifically,the interference of background noise is almost completely removed on a microfluidic chip by isolating microbeads through acoustic radiation force,on which the biomarkers are captured by the immobilized detection probe.And then,the concentration of cTnI in human serum is detected by the fluorescence intensity change of the isolated functionalized beads.By this way,the detection limit of our biosensor calculated by 3σ/K method is 44 pg/mL and 0.34 ng/mL in PBS buffer and human serum respectively.Finally,the reliability of this method has been validated by comparison with clinical tests from the nephelometric analyzer in hospital.

关键词： cardiac separation noise

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Fast frequency relocking for synchronization enhanced resonant accelerometer

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Microsystems & Nanoengineering 2022年第5期8卷 53-60页

作者： Liu Xu Yonghong Qi Zhuangde Jiang xueyong wei State Key Laboratory for Manufacturing Systems Engineering Xi’an Jiaotong UniversityXi’anShaanxi 710049China

Synchronization,as a unique phenomenon,has been extensively studied in biology,chaotic systems,nonlinear dynamics,quantum information,and other fields.Benefiting from the characteristics of frequency amplification,noise suppression,and stability improvement,synchronization has been gradually applied in sensing,communication,time keeping,and other applications.In the sensing field,synchronization provides a new strategy to improve the performance of sensors.However,the performance improvement is only effective within the synchronization range,and the narrow synchronization range has become a great challenge for the wide application of synchronizationenhanced sensing mechanism.Here,we propose a frequency automatic tracking system(FATS)to widen the synchronization range and track the periodic acceleration signals by adjusting the frequency of the readout oscillator in real time.In addition,a high-precision frequency measurement system and fast response control system based on FPGA(Field Programmable Gate Array)are built,and the tracking performance of the FATS for static and dynamic external signals is analyzed to obtain the optimal control parameters.Experimental results show that the proposed automatic tracking system is capable of static acceleration measurement,the synchronization range can be expanded to 975 Hz,and the relocking time is shortened to 93.4 ms at best.By selecting the optimal PID parameters,we achieve a faster relocking time to meet the requirements of low-frequency vibration measurements,such as seismic detection and tidal monitoring.

关键词： synchronization locking acceleration

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Single-electron detection utilizing coupled nonlinear microresonators

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Microsystems & Nanoengineering 2020年第1期6卷 327-333页

作者： Xuefeng Wang xueyong wei Dong Pu Ronghua Huan Department of Mechanics Key Laboratory of Soft Machines and Smart Devices of Zhejiang ProvinceZhejiang UniversityHangzhou 310027People’s Republic of China State Key Laboratory for Manufacturing Systems Engineering Xi’an Jiaotong UniversityXi’an 710049People’s Republic of China

Since the discovery of the electron,the accurate detection of electrical charges has been a dream of the scientific community.Owing to some remarkable advantages,micro/nanoelectromechanical system-based resonators have been used to design electrometers with excellent sensitivity and resolution.Here,we demonstrate a novel ultrasensitive charge detection method utilizing nonlinear coupling in two micromechanical resonators.We achieve single-electron charge detection with a high resolution up to 0.197±0.056 e=/√Hz Hz p at room temperature.Our findings provide a simple strategy for measuring electron charges with extreme accuracy.

关键词： temperature utilizing nonlinear

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Programmable synchronization enhanced MEMS resonant accelerometer

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Microsystems & Nanoengineering 2020年第1期6卷 654-663页

作者： Liu Xu Shudong Wang Zhuangde Jiang xueyong wei State Key Laboratory for Manufacturing Systems Engineering Xi’an Jiaotong UniversityXi’an 710049China

Acceleration measurement is of great significance due to its extensive applications in military/industrial fields.In recent years,scientists have been pursuing methods to improve the performance of accelerometers,particularly through seeking new sensing mechanisms.Herein,we present a synchronized oscillator-based enhancement approach to realize a fivefold resolution improvement of a microelectromechanical resonant accelerometer.Through the unidirectional electrical coupling method,we achieved synchronization of the sensing oscillator of the microelectromechanical resonant accelerometer and an external reading oscillator,which remarkably enhanced the stability of the oscillation system to 19.4 ppb and the resolution of the accelerometer to 1.91μg.In addition,the narrow synchronization bandwidth of conventional synchronized oscillators was discussed,and hence,we propose a novel frequency automatic tracking system to expand the synchronization bandwidth from 113 to 1246 Hz,which covers the full acceleration measurement range of±1 g.For the first time,we utilized a unidirectional electrical synchronization mechanism to improve the resolution of resonant sensors.Our comprehensive scheme provides a general and powerful solution for performance enhancement of any microelectromechanical system(MEMS)resonant sensor,thereby enabling a wide spectrum of applications.

关键词： resonant oscillator hence

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Multiple virus sorting based on aptamer-modified microspheres in a TSAW device

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微系统与纳米工程(英文) 2023年第3期9卷 309-318页

作者： Xianglian Liu Xuan Chen Yangchao Dong Chuanyu Zhang Xiaoli Qu Yingfeng Lei Zhuangde Jiang xueyong wei State Key Laboratory for Manufacturing Systems Engineering Xi’an Jiaotong UniversityXi’an 710049China Department of Microbiology the Fourth Military Medical UniversityXi’an 710032China

Due to the overlapping epidemiology and clinical manifestations of flaviviruses,differential diagnosis of these viral diseases is complicated,and the results are unreliable.There is perpetual demand for a simplified,sensitive,rapid and inexpensive assay with less cross-reactivity.The ability to sort distinct virus particles from a mixture of biological samples is crucial for improving the sensitivity of diagnoses.Therefore,we developed a sorting system for the subsequent differential diagnosis of dengue and tick-borne encephalitis in the early stage.We employed aptamer-modified polystyrene(PS)microspheres with different diameters to specifically capture dengue virus(DENV)and tick-borne encephalitis virus(TBEV),and utilized a traveling surface acoustic wave(TSAW)device to accomplish microsphere sorting according to particle size.The captured viruses were then characterized by laser scanning confocal microscopy(LSCM),field emission scanning electron microscopy(FE-SEM)and reverse transcription-polymerase chain reaction(RT‒PCR).The characterization results indicated that the acoustic sorting process was effective and damage-free for subsequent analysis.Furthermore,the strategy can be utilized for sample pretreatment in the differential diagnosis of viral diseases.

关键词： diagnosis epidemiology sorting

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Frequency unlocking-based MEMS bifurcation sensors

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微系统与纳米工程(英文) 2023年第3期9卷 171-181页

作者： Yan Qiao Zhan Shi Yutao Xu xueyong wei Alaaeldin Elhady Eihab Abdel-Rahman Ronghua Huan Wenming Zhang State Key Laboratory of Mechanical System and Vibration School of Mechanical EngineeringShanghai Jiao Tong UniversityShanghaiChina Department of Mechanics Key Laboratory of Soft Machines and Smart Devices of Zhejiang ProvinceZhejiang UniversityHangzhouChina State Key Laboratory for Manufacturing Systems Engineering Xi’an Jiaotong UniversityXi’anChina Department of Systems Design Engineering University of WaterlooWaterlooONCanada

MEMS resonators exhibit rich dynamic behaviors under the internal resonance regime. In this work, we present a novel MEMS bifurcation sensor that exploits frequency unlocking due to a 1:3 internal resonance between two electrostatically coupled micro-resonators. The proposed detection mechanism allows the sensor to operate in binary (digital) and analog modes, depending on whether the sensor merely detects a significant jump event in the peak frequency upon unlocking or measures the shift in the peak frequency after unlocking and uses it in conjunction with a calibration curve to estimate the corresponding change in stimulus. We validate the success of this sensor paradigm by experimentally demonstrating charge detection. High charge resolutions are achieved in binary mode, up to 0.137 fC, and in analog mode, up to 0.01 fC. The proposed binary sensor enables extraordinarily high detection resolutions due to the excellent frequency stability under internal resonance and the high signal-to-noise ratio of the shift in peak frequency. Our findings offer new opportunities for high-performance ultrasensitive sensors.

关键词： locking bifurcation binary

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1980—2016年中国北方风蚀气候侵蚀力的变化

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地理科学 2024年第4期44卷 703-710页

作者：刘伟邹学勇刘博王汝幸王祖正天津师范大学地理学部天津300387 北京师范大学地理科学学部北京100875 中国环境科学研究院北京100012 合肥大学旅游与会展学院安徽合肥230601

随着修订风蚀方程(RWEQ)的提出,采用增加了冻结因子的气候因子(WF)表达风蚀气候侵蚀力更具有科学性和必要性。基于中国北方风蚀区157个气象站1980—2016年的观测数据,逐月计算了各站点气候因子,并探讨了气候因子时空分布变化特征及影响... 详细信息

随着修订风蚀方程(RWEQ)的提出,采用增加了冻结因子的气候因子(WF)表达风蚀气候侵蚀力更具有科学性和必要性。基于中国北方风蚀区157个气象站1980—2016年的观测数据,逐月计算了各站点气候因子,并探讨了气候因子时空分布变化特征及影响因素。结果表明:①1980—1997年,年气候因子值表现为快速下降,从940.46 kg/m下降到273.03 kg/m;而1998—2016年,年气候因子值表现相对稳定,在139.81~398.85 kg/m范围内波动。②春季气候因子值约占全年63%,其变化也显著高于其它季节,这也是土壤风蚀发生在春季的主要原因。③气候因子的高值区分布在新疆东部、青海西部、40°N以北的内蒙古中西部,这与气压中心和高大山体地形分布有关。

关键词：气候因子土壤风蚀全球变暖北方风蚀区

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Robot-aided fN∙m torque sensing within an ultrawide dynamic range

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Microsystems & Nanoengineering 2021年第1期7卷 51-61页

作者： Shudong Wang xueyong wei Haojian Lu Ziming Ren Zhuangde Jiang Juan Ren Zhan Yang Lining Sun Wanfeng Shang Xinyu Wu Yajing Shen State Key Laboratory for Manufacturing Systems Engineering Xi’an Jiaotong UniversityXi’an 710049China Mechanical and Biomedical Engineering Department City University of Hong KongHong Kong SAR 999077China Department of Mechatronics Chang’an UniversityXi’an 710054China Robotics and Microsystems Center Soochow UniversitySuzhou 215021China Shenzhen Institutes of Advanced Technology Chinese Academy of SciencesShenzhen 518055China Shenzhen Research Institute City University of Hong KongShenzhen 440305China

In situ scanning electron microscope(SEM)characterization have enabled the stretching,compression,and bending of micro/nanomaterials and have greatly expanded our understanding of small-scale phenomena.However,as one of the fundamental approaches for material analytics,torsion tests at a small scale remain a major challenge due to the lack of an ultrahigh precise torque sensor and the delicate sample assembly strategy.Herein,we present a microelectromechanical resonant torque sensor with an ultrahigh resolution of up to 4.78 fN∙m within an ultrawide dynamic range of 123 dB.Moreover,we propose a nanorobotic system to realize the precise assembly of microscale specimens with nanoscale positioning accuracy and to conduct repeatable in situ pure torsion tests for the first time.As a demonstration,we characterized the mechanical properties of Si microbeams through torsion tests and found that these microbeams were five-fold stronger than their bulk counterparts.The proposed torsion characterization system pushes the limit of mechanical torsion tests,overcomes the deficiencies in current in situ characterization techniques,and expands our knowledge regarding the behavior of micro/nanomaterials at various loads,which is expected to have significant implications for the eventual development and implementation of materials science.

关键词： torsion precise dynamic

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健康女性月经前后太冲及太溪两穴红外光谱分析

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Journal of Acupuncture and Tuina Science 2024年第2期22卷 160-166页

作者：郝英博马斯琪沈雪勇魏建子杨燕萍(翻译) Shanghai University of Traditional Chinese Medicine Shanghai 201203China 不详

目的:观察月经前后太冲、太溪两穴及对照点的红外辐射变化,探究生理性月经相关脏腑原穴的红外辐射特性。方法:穴位红外辐射光谱检测系统检测32例成年健康女性月经前、月经期、月经后三个时期太冲、太溪及两穴旁开1 cm处对照点在波长为1.... 详细信息

目的:观察月经前后太冲、太溪两穴及对照点的红外辐射变化,探究生理性月经相关脏腑原穴的红外辐射特性。方法:穴位红外辐射光谱检测系统检测32例成年健康女性月经前、月经期、月经后三个时期太冲、太溪及两穴旁开1 cm处对照点在波长为1.50-18.00μm范围内的红外辐射光谱,进行数据挖掘与分析。结果:月经前、月经期和月经后双侧太冲、太溪及其对照点的红外光谱形态基本一致,红外光谱波长位于11.25μm处;经前右太溪红外辐射总强度显著低于对照点(P<0.05),左太冲红外辐射总强度显著低于对照点(P<0.01);月经期和月经后,双侧太溪红外辐射总强度均显著低于对照点(P<0.05);穴位与对照点红外辐射强度有差异的波长点集中于7.50-14.25μm的主波峰和15.00-17.25μm的次波峰处。结论:月经不同时期太冲、太溪两穴红外辐射光谱具有明显的穴位特异性,主要体现在红外辐射强度及红外辐射波长方面。

关键词：穴位特异性穴,太冲穴,太溪原穴月经周期女性

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2019—2022年河南省肾综合征出血热宿主动物监测情况分析

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中华预防医学杂志 2024年第1期58卷 18-24页

作者：李东晓范威朱琳胡晓李懿马红霞王海峰叶莹苏佳黄学勇河南省疾病预防控制中心传染病预防控制所郑州450016

目的分析2019—2022年河南省肾综合征出血热(HFRS)宿主动物分布及携带汉坦病毒(HV)情况。方法在河南省HFRS监测点开展宿主动物监测,采用实时荧光定量PCR法检测鼠肺HV,分析河南省鼠类动物携带HV型别,对阳性样本进行测序,分析核苷酸序列... 详细信息

目的分析2019—2022年河南省肾综合征出血热(HFRS)宿主动物分布及携带汉坦病毒(HV)情况。方法在河南省HFRS监测点开展宿主动物监测,采用实时荧光定量PCR法检测鼠肺HV,分析河南省鼠类动物携带HV型别,对阳性样本进行测序,分析核苷酸序列的同源性及变异情况。结果2019—2022年共捕鼠1308只,16份鼠肺HV核酸阳性,鼠HV阳性率为1.22%(16/1308)。按种类,黑线姬鼠HV阳性率最高(68.75%,11/16)。按分布地,野外鼠HV阳性率最高(2.50%,12/480),居民区鼠HV阳性率为0.53%(4/759)。16份阳性样本分型结果显示,所携带的病毒均属于Ⅰ型汉滩病毒。阳性样本测序后获得8条S基因片段(OQ681444~OQ681451)和6条M基因片段(OQ681438~OQ681443);S和M基因片段与陕西84FLi株、四川SN7株相似性较高。S和M基因片段系统进化分析显示,均为汉滩病毒H5基因亚型。氨基酸序列分析显示,与汉滩病毒疫苗株84FLi相比,8条S片段编码氨基酸第74位由天冬酰胺替换为丝氨酸;XC2022047株在Gn区第14位由色氨酸替换为甘氨酸,XC2022022和XC2022024第359位由异亮氨酸替换为丙氨酸。结论2019—2022年河南省宿主动物携带HV属于Ⅰ型汉滩病毒,黑线姬鼠仍然是汉滩病毒的优势宿主。同疫苗株比较,S和M基因片段的免疫表位区均有氨基酸位点替换。

关键词：肾综合征出血热监测汉坦病毒黑线姬鼠汉滩病毒

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