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Experimental Study on the Axial Compression Performance of Bamboo Scrimber Columns Embedded with Steel Reinforcing Bars

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Structural Durability & Health Monitoring 2024年第6期18卷 805-833页

作者： xueyan lin Mingtao Wu Guodong Li Nan Guo Lidan Mei College of Civil Engineering and Transportation Northeast Forestry UniversityHarbin150000China Department of Building Engineering College of Civil EngineeringTongji UniversityShanghai200092China College of Transportation and Civil Engineering Fujian Agriculture and Forestry UniversityFuzhou350002China

In this paper,a new type of bamboo scrimber column embedded with steel bars(rebars)was proposed,and the compression performance was improved by pre-embedding rebars during the preparation of the *** effects of the slenderness ratio and the reinforcement ratio on the axial compression performance of reinforced bamboo scrimber columns were studied by axial compression tests on 28 *** results showed that the increase in the slenderness ratio had a significant negative effect on the axial compression performance of the *** the slenderness ratio increased from 19.63 to 51.96,the failure mode changed from strength failure to buckling failure,and the maximum bearing capacity decreased by 43.03%.The axial compression performance of the reinforced bamboo scrimber columns did not significantly improve at a slenderness ratio of 19.63,but the opposite was true at slenderness ratios of 36.95 and *** the reinforcement ratio increased from 0%to 4.52%,the bearing capacity of those with a slenderness ratio of 51.96 increased by up to 16.99%,and the stiffness and ductility were also ***,based on existing specifications,two modification parameters,the overall elastic modulus Ec and the combined strength fcc,were introduced to establish a calculation method for the bearing capacity of the reinforced bamboo scrimber *** calculation results were compared with the test results,and the results showed that the proposed calculation models can more accurately predict the bearing capacity.

关键词： Bamboo scrimber column steel bar axial compression performance theoretical model

Responses in splanchnic and mammary amino acid metabolism to short-term graded removal of methionine in lactating goats

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Animal Nutrition 2023年第2期13卷 116-125页

作者： Yantao Li Mark D.Hanigan xueyan lin Zhiyong Hu Zhengui Yan Qiuling Hou Yun Wang Zhonghua Wang Ruminant Nutrition and Physiology Laboratory College of Animal Science and TechnologyShandong Agricultural UniversityTai'an 271018China Department of Dairy Science Virginia TechBlacksburg 24061USA

Four multi-catheterized lactating goats were used in a 4×4 Latin square experiment to investigate the responses of amino acid metabolism in portal-drained viscera(PDV),liver,and mammary glands to short-term varying supplies of methionine(Met).During the last 45 h in each experimental period,goats were fasted for 12 h and then abomasally infused with an amino acid(AA)mixture plus glucose for 33 *** consisted of graded removal of Met from an infused AA mixture to achieve Met content in the infusate of 100%(complete),60%,30%,or 0%that in *** Met removal decreased the pro-duction of milk,milk protein,lactose,and fat linearly whilst also decreasing arterial Met concentration linearly(Plinearly(P0.1)was maintained as Met supply *** was achieved through increased mammary affinity(Plinearly decreased mammary uptake-to-milk output ratios of Met(Plinearly(0.05linearly decreased circulating concentration of prolactin and linearly increased insulin concentration(Plinear increase in mammary blood *** observed decreases in milk protein production as Met supply decreased appear to be a result of regulatory events which may have been driven by decreased circulating prolactin,rather than as a result of decreased mammary Met uptake.

关键词： Methionine Lactating goat Milk protein Amino acid Splanchnic tissue

Relationship between FHIT Expression and HPV Status in Vulvar Squamous Cell Carcinomas

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Chinese Journal of Clinical Oncology 2009年第1期6卷 68-71页

作者： Bo Li Caigang Liu ling Ou- Yang xueyan lin Lili Chen Shulan Zhang Department of Obstetric and Gynecology Shengjing Hospital of China Medical University Shenyang 110004 Liaoning Province China Department of Oncology The First Affiliated Hospital of China Medial University Shenyang 110001 Liaoning Province China.

OBJECTIVE To investigate the relationship between fragilehistidine triad (FHIT) depletion and human papillomavirus (HPV)status in the vulvar squamous cell carcinomas (VSCC).METHODS Immunohistochemical method was used to detectthe expression of FHIT protein in 42 cases of VSCC and 10 casesof normal vulvar tissues. PCR was used to detect HPV infectionstatus. We analyzed the relationship of the expression of FHITprotein between the 2 groups: HPV positive and HPV negative, aswell as the clinically pathological *** The expression of FHIT was positive in all normal vul-var tissues, and 71.4% was depletion in VSCC (P < 0.01). Abnormalexpression of FHIT was significantly correlated with pathologicalgrade (P < 0.05). There was a significant difference between FHITdepletion and HPV infection based on the statistial analysis (P <0.05).CONCLUSION The FHIT depletion is related to occurrenceand development of VSCC, and the abnormal expression of FHITsignificantly correlates with HPV infection.

关键词： fragile histidine triad vulvar squamous cell carcinoma human papillomavirus.

Preparation of a polyclonal antibody against immunogenic fragment of bovine intestinal peptide transporter I (bPepTI)

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Advances in Bioscience and Biotechnology 2012年第2期3卷 137-142页

作者： Xiuxin Zhao xueyan lin Guimei Liu Zhonghua Wang College of Animal Science and Technology Shandong Agricultural University Tai’an China

The goal of the current study is to prepare polyclonal antibody against bovine intestinal peptide transporter I (bPepTI) in order to develop assay for immunological assessment of protein levels. Antigenicity of the entire bPepTI was analyzed with DNAStar, and a fragment with high antigenicity (bPepTI ORF 1369 - 1695) was selected, cloned in pGEX-6p-1 vector, resulting in a recombinant plasmid GST-BP, which verified by double enzyme digestion and sequenced, the recombinant plasmid was introduced to BL21. Exogenous expression was induced by IPTG and validated by Western blot analysis. The recombinant protein was isolated, purified and used for production of antiserum in mice. The specificity of antiserum was evaluated with immunobloting and titer was estimated with ELISA. Results indicate that the antibody against bPepTI was produced. The optimal GST-BP antigen embedding concentration was 0.5 μg/ml. The optimal dilution was 1:400. An indirect ELISA assay indicates the effective dilution was 1:102400.

关键词： Bovine Type I Peptide Transporter Clone Prokaryotes Expression Antibody Preparation

Choice for host-specific high-adhesive Lactobacillusstrains

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Advances in Bioscience and Biotechnology 2012年第2期3卷 149-152页

作者： xueyan lin Zhonghua Wang Zhongxiang Niu Hongjie Liu Yun Wang College of Animal Science and Technology Shandong Agricultural University Tai’an China

Adhesive ability was tested in seven Lactobacillus solated from the chicken digestive tract after cultivation with CaCo-2 cells (intestinal epithelial cells), MDCK (dog kidney) cells and CEF (chicken embryo fiber) cells. We noted the following important observations regarding the adhesive ability between different Lactobacillus strains and three cell types: the adhesive interaction between the SDnB7, SDnE1 and SDnA3 lactobacillus strains and CaCo-2cells was greater compared to controls, the adhesive effect between SDnB1 and CEF cells was also greater than controls and Lactobacillus showed only minimal adherence to MDCK cells. Incubation time also affected Lactobacillus adherence to CaCo-2cells: adhesive ability was optimal at 37°C when incubated for 2 days and this was confirmed by a local increase in the concentration of Lactobacillus around CaCo-2 cells when incubated for 24 h as opposed to 3 h. Adherence ability in lactobacillus was also tested at various concentrations (108, 107, 106, 105 and 104 ). The number of Lactobacillus that adhered around the cells was significantly increased in the treatment with 108 bacterial cells. Transmission electron microscopy revealed that the cellularity of the junction between CaCo-2cells and Lactobacillus was not compromised. Transmission electron microscopy also revealed that the thalli fabric structure remained intact.

关键词： Host Specific CaCo-2Cells MDCK Cells CEF Cells High Adhesion Lactobacillus

The nature of adhesion factors which lie on the surfaces of Lactobacillusadhering to cells

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Advances in Bioscience and Biotechnology 2012年第2期3卷 153-157页

作者： xueyan lin Zhonghua Wang Zhongxiang Niu Jun Peng Yun Wang College of Animal Science and Technology Shandong Agricultural University Tai’an China

Lactobacillus adheres to intestinal epithelial cells and yeast fungus cells with the aid of adhesion factors expressed on its cell surface. To identify adhesion factors nature on the surface of Lactobacillus, an adhesion experiment was carried out by pre-treating the Lactobacillus supernatant with different concentrations of bovine serum albumin, trypsin and 100°C for 10min. Additionally, intestinal epithelial cells were treated with sodium iodate, trypsin and sugar inhibition tests to characterize the receptors in Lactobacillus that interact with intestinal epithelial cells. It was demonstrated that Lactobacillus adhesion ability was decreased (P Lactobacillus cells was identified as a D-mannose glycoprotein. This observation was confirmed after treating intestinal epithelial cells with sodium iodate and trypsin, and sugar inhibition tests. Wild type Lactobacillus can agglutinate yeast fungus cells but after being exposured to mannose, agglutination to yeast fungus cells is lost or reduced. Results from this study we also got that inactivated and live bacteria that similarly adhere to intestinal epithelial cells.

关键词： Lactobacillus Adhesion Intestinal Epithelial Cells Adhesion Factors Nature

Metallothionein-Null mouse exhibits reduced tolerance to ultraviolet B injury in vivo

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Metallothionein-Null mouse exhibits reduced tolerance to ult...

Effects of Supplemental Feeding of Probiotics during Lactation on Rumen Microflora of Calves after Weaning

International Forum 2003 Academic Conference of Dermatology of the Integration of TCM with Western Medicine

作者： Wang Wenhui, Zhang Baoxu, Lu xueyan, Feng lin

Background: Events that induce expression of metallothionein （MT）gene, such as injection of cadmium chloride, cold stress and topical applica-tion of 1.25 dihydroxyvitamin D, can deplete the number of UVB-inducedsunburn cells in mouse skin in vivo. MT-null mouse skin explants exhibitedreduced tolerance to UVB injury in vitro. However, the in vivo response ofMT-null mouse to UVB injury has not been investigated.

关键词： gene, knock out, metallothionein, mouse, sunburn cell,UVB

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Advances in Bioscience and Biotechnology 2021年第7期12卷 213-228页

作者： xueyan lin Tian Zhang lin Ju Yue Jiang Qiuling Hou Zhiyong Hu Yun Wang Zhonghua Wang College of Animal Science Shandong Agricultural University Tai’an China

For the experiment, 8 newborn male Holstein calves were selected that had the same feeding environment, and were of similar ages. They were randomly divided into 2 groups, with 4 in each group. The treatments consisted of feeding active probiotics (Group P) and a normal fed control group (Group C). The growth performance and blood indices were measured;rumen fluid samples were collected after weaning, and 16SrDNA sequencing and LC-MS metabolome detection were performed. Compared with the control group, relative abundances of Deltaproteobacteria, Desulfovibrionales, Bacteroidales_ BS11_gut_group, Desulfovibrionaceae, Bacteroidales_S24-7_group, Acetobacteraceae, Ruminococcaceae_NK4A214_group, Asaia, [Ruminococcus] gauvreauii_group, Desulfovibrio, Kingella, Selenomonas, Lachnoclostridium in group P were significantly different (P of 2-methylbenzoic acid and myo-inositol were significantly increased (P 0.05). These results showed that compared with normally fed calves, the growth performance and blood indices of probiotic-fed calves were changed, but the differences were not signif

关键词： Calves Probiotics Rumen Fluid Serum Metabolites

Effects of Supplementation with Saccharomyces cerevisiaeand Its Fermentation Products on Production Performance and Its Mechanism in Perinatal Dairy Cows

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Advances in Bioscience and Biotechnology 2021年第7期12卷 193-212页

作者： xueyan lin Ke Li lin Ju Xu Hao Yue Jiang Qiuling Hou Zhiyong Hu Yun Wang Zhonghua Wang College of Animal Science Shandong Agricultural University Tai’an China

The aim of this study was to investigate the effects of Saccharomyces cerevisiae and its fermentation products on performance, blood hormone levels and rumen floral composition in peripartum dairy cows. Sixty perinatal cows were selected and allocated to two groups according to parity and expected date of delivery. Each group was supplemented with Saccharomyces cerevisiae and its fermentation product 0 or 100 g. The results showed that Saccharomyces cerevisiae and its fermentation products could significantly increase the feed intake of peripartum dairy cows (P β-hydroxybutyrate (P = 0.01), reducing the insulin content (P = 0.02). Saccharomyces cerevisiae reduced the abundance of rumen microbes in peripartum dairy cows but had no effect on rumen microbial diversity. Compared with the control group, the supplemented group showed reductions in the abundance of genera Bacillus (P = 0.03), Butyrivibrio (P = 0.01),

关键词： Saccharomyces cerevisiae Perinatal Period Dairy Cows Production Performance Mechanism