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BAK1 plays contrasting roles in regulating abscisic acid-induced stomatal closure and abscisic acid-inhibited primary root growth in Arabidopsis

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Journal of Integrative Plant Biology 2022年第6期64卷 1264-1280页

作者： Jinping Deng Lingyao Kong Yinhua Zhu Dan Pei xuexue chen Yu Wang Junsheng Qi Chunpeng Song Shuhua Yang Zhizhong Gong State Key Laboratory of Plant Physiology and Biochemistry College of Biological SciencesChina Agricultural UniversityBeijing 100193China College of Life Sciences Qingdao UniversityQingdao 266071China Collaborative Innovation Center of Crop Stress Biology Institute of Plant Stress BiologyHenan UniversityKaifeng 475001HenanChina Institute of Life Science and Green Development School of Life SciencesHebei UniversityBaoding 071002China

The mechanisms that balance plant growth and stress responses are poorly understood, but they appear to involve abscisic acid(ABA) signaling mediated by protein kinases. Here, to explore these mechanisms, we examined the responses of Arabidopsis thaliana protein kinase mutants to ABA treatment. We found that mutants of BRASSINOSTEROID INSENSITIVE 1-ASSOCIATED RECEPTOR KINASE 1(BAK1) were hypersensitive to the effects of ABA on both seed germination and primary root growth. The kinase OPEN STOMATA 1(OST1) was more highly activated by ABA in bak1 mutant than the wild type. BAK1 was not activated by ABA treatment in the dominant negative mutant abi1-1 or the pyr1 pyl4 pyl5 pyl8 quadruple mutant, but it was more highly activated by this treatment in the abi1-2 abi2-2 hab1-1 loss-of-function triple mutant than the wild type. BAK1 phosphorylates OST1 T146 and inhibits its activity. Genetic analyses suggested that BAK1 acts at or upstream of core components in the ABA signaling pathway, including PYLs, PP2 Cs,and Sn RK2 s, during seed germination and primary root growth. Although the upstream brassinosteroid(BR) signaling components BAK1 and BR INSENSITIVE 1(BRI1) positively regulate ABAinduced stomatal closure, mutations affecting downstream components of BR signaling, including BRASSINOSTEROID-SIGNALING KINASEs(BSKs)and BRASSINOSTEROID-INSENSITIVE 2(BIN2), did not affect ABA-mediated stomatal movement. Thus,our study uncovered an important role of BAK1 in negatively regulating ABA signaling during seed germination and primary root growth, but positively modulating ABA-induced stomatal closure, thus optimizing the plant growth under drought stress.

关键词： abscisic acid BAK1 primary root growth stomatai closure

Vacuolar Phosphate Transporter1 (VPT1) may transport sugar in response to soluble sugar status of grape fruits

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Horticulture Research 2023年第2期10卷 94-105页

作者： Qian Bai xuexue chen Zhenzhen Zheng Jinjing Feng Yanjun Zhang Yuanyue Shen Yun Huang Beijing Key Laboratory for Agricultural Application and New Technique College of Plant Science and TechnologyBeijing University of AgricultureBeijing 102206China Ministry of Education Key Laboratory of Silviculture and Conservation College of ForestryBeijing Forestry University35 East Qinghua RoadBeijing 100083China

Vacuolar Phosphate Transporter1(VPT1)-mediated phosphate uptake in the vacuoles is essential to plant development and fruit ripening.Interestingly,here we find that the VPT1 may transport sugar in response to soluble sugar status of fruits.The VvVPT1 protein isolated from grape(Vitis vinifera)berrieswas tonoplast-localized and contains SPX(Syg1/Pho81/XPR1)and MFS(major facilitator superfamily)domains.Its mRNA expression was significantly increased during fruit ripening and induced by sucrose.Functional analyses based on transient transgenic systems in grape berry showed that VvVPT1 positively regulated berry ripening and significantly affected hexose contents,fruit firmness,and ripening-related gene expression.The VPT1 proteins(Grape VvVPT1,strawberry FaVPT1,and Arabidopsis AtVPT1)all showed low affinity for phosphate verified in yeast system,while they appear different in sugar transport capacity,consistent with fruit sugar status.Thus,our findings reveal a role for VPT1 in fruit ripening,associated to its SPX and MFS domains in direct transport of soluble sugar available into the vacuole,and open potential avenues for genetic improvement in fleshy fruit.

关键词： sugar fir transport

Protein kinases in plant responses to drought, salt,and cold stress

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Journal of Integrative Plant Biology 2021年第1期63卷 53-78页

作者： xuexue chen Yanglin Ding Yongqing Yang Chunpeng Song Baoshan Wang Shuhua Yang Yan Guo Zhizhong Gong State Key Laboratory of Plant Physiology and Biochemistry College of Biological SciencesChina Agricultural UniversityBeijing 100193China Collaborative Innovation Center of Crop Stress Biology Henan ProvinceInstitute of Plant Stress BiologyHenan UniversityKaifeng 475001China Key Lab of Plant Stress Research College of Life ScienceShandong Normal UniversityJi'nan 250000China Institute of Life Science and Green Development School of Life SciencesHebei UniversityBaoding 071001China

Protein kinases are major players in various signal transduction pathways. Understanding the molecular mechanisms behind plant responses to biotic and abiotic stresses has become critical for developing and breeding climate-resilient crops. In this review,we summarize recent progress on understanding plant drought, salt, and cold stress responses, with a focus on signal perception and transduction by different protein kinases, especially sucrose nonfermenting1(SNF1)-related protein kinases(Sn RKs),mitogen-activated protein kinase(MAPK) cascades,calcium-dependent protein kinases(CDPKs/CPKs),and receptor-like kinases(RLKs). We also discuss future challenges in these research fields.

关键词： CDPKs cold stress drought stress MAPK cascade RLKs salt stress Sn RKs

Arabidopsis U-box E3 ubiquitin ligase PUB11 negatively regulates drought tolerance by degrading the receptor-like protein kinases LRR1 and KIN7

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Journal of Integrative Plant Biology 2021年第3期63卷 494-509页

作者： xuexue chen Tingting Wang Amin Ur Rehman Yu Wang Junsheng Qi Zhen Li Chunpeng Song Baoshan Wang Shuhua Yang Zhizhong Gong State Key Laboratory of Plant Physiology and Biochemistry College of Biological SciencesChina Agricultural UniversityBeijing 100193China Collaborative Innovation Center of Crop Stress Biology Institute of Plant Stress BiologyHenan UniversityKaifeng 475001China Key Lab of Plant Stress Research College of Life ScienceShandong Normal UniversityJi'nan 250000China School of Life Sciences Institute of Life Science and Green DevelopmentHebei UniversityBaoding 100193China

Both plant receptor-like protein kinases(RLKs)and ubiquitin-mediated proteolysis play crucial roles in plant responses to drought stress.However,the mechanism by which E3 ubiquitin ligases modulate RLKs is poorly understood.In this study,we showed that Arabidopsis PLANT U-BOX PROTEIN 11(PUB11),an E3 ubiquitin ligase,negatively regulates abscisic acid(ABA)-mediated drought responses.PUB11 interacts with and ubiquitinates two receptor-like protein kinases,LEUCINE RICH REPEAT PROTEIN 1(LRR1)and KINASE 7(KIN7),and mediates their degradation during plant responses to drought stress in vitro and in vivo.pub11 mutants were more tolerant,whereas Irr1 and kin7 mutants were more sensitive,to drought stress than the wild type.Genetic analyses show that the pub11 Irr1 kin7 triple mutant exhibited similar drought sensitivity as the Irr1 kin7 double mutant,placing PUB11 upstream of the two RLKs.Abscisic acid and drought treatment promoted the accumulation of PUB11,which likely accelerates LRR1 and KIN7 degradation.Together,our results reveal that PUB11 negatively regulates plant responses to drought stress by destabilizing the LRR1 and KIN7 RLKs.

关键词： abscisic acid drought response E3 ligase receptor-like kinase ubiquitination

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马克思恩格斯文明观多维论析

浙江学刊 2022年第6期 133-140页

作者：陈雪雪上海理工大学马克思主义学院上海200093

马克思恩格斯文明观意涵丰富,主要体现在五个维度。其一,作为生产方式的文明。农业和工业的生产方式分别造就了农业的和工业的文明形式。资本文明是一种典型的工业文明,但更高级的生产方式及文明形态构成人类历史的真正出路。其二,作为... 详细信息

马克思恩格斯文明观意涵丰富,主要体现在五个维度。其一,作为生产方式的文明。农业和工业的生产方式分别造就了农业的和工业的文明形式。资本文明是一种典型的工业文明,但更高级的生产方式及文明形态构成人类历史的真正出路。其二,作为社会形态的文明。人类社会经历不同发展形态,具有与之相应的文明类型。“自由个性”是人类社会的高级阶段,真正的普遍的文明在此阶段呈现。其三,作为时代历史阶段的文明。文明是区分不同时代的鲜明标识,代表时代历史的发展深度。文明时代与野蛮时代、蒙昧时代区别开来。其四,作为历史演进趋势的文明。人类历史不断由低级阶段步入高级阶段,文明不断从资本文明挺进社会主义与共产主义的高级文明。其五,作为人的生存状态的文明。文明的本质在于人的进步。人的理想生存状态是实现“人的生命活动的性质”,全面占有自己的本质。

关键词：马克思恩格斯文明观文明形态

转录因子FabHLH148参与草莓果实的颜色发育

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果树学报 2022年第8期39卷 1358-1367页

作者：郑珍珍陈雪雪沈元月黄芸北京农学院植物科学技术学院·农业应用新技术北京市重点实验室北京102206

【目的】探究碱性螺旋-环-螺旋(basic helix-loop-helix,bHLH)转录因子在草莓果实成熟过程中的功能。【方法】基于转录组测序和基因组数据库,克隆FabHLH148基因;分析其理化性质、保守结构域、预测编码的蛋白质结构、系统进化树、亚细胞... 详细信息

【目的】探究碱性螺旋-环-螺旋(basic helix-loop-helix,bHLH)转录因子在草莓果实成熟过程中的功能。【方法】基于转录组测序和基因组数据库,克隆FabHLH148基因;分析其理化性质、保守结构域、预测编码的蛋白质结构、系统进化树、亚细胞定位等;采用RT-qPCR检测其在草莓中的时空表达水平,并构建过表达(over expression,OE)和RNA干扰(RNAinterference,RNAi)载体,利用农杆菌介导瞬时侵染草莓果实,观察记录表型,检测FabHLH148表达水平,并测定花色素苷含量。【结果】FabHLH148基因CDS区全长687 bp,编码228个氨基酸,预测蛋白分子质量24.89 ku,理论等电点(pI)为11.65;该基因属于bHLH超家族,因其与二倍体草莓FvbHLH148序列相似度最高,所以将其命名为FabHLH148;亚细胞定位显示,FabHLH148主要定位在细胞核。RT-qPCR结果表明,FabHLH148在草莓不同组织部位均有表达,在果实中有较高表达并随果实成熟表达量显著升高。过表达FabHLH148能够促进果实着色以及花色素苷的积累,通过RNAi调低Fab-HLH148的表达则作用结果相反,且FabHLH148在OE组果实中表达水平显著高于对照,RNAi组果实中表达水平显著低于对照(p<0.01)。【结论】转录因子FabHLH148属于bHLH超家族,可促进草莓果实着色。

关键词：草莓 FabHLH148 转录因子表达模式花色素苷

常压室温等离子诱变筛选高产酯酶的菠萝蜜果酒酿酒酵母

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食品与发酵工业 2022年第22期48卷 110-118页

作者：张雪吴叶陈雪雪陈星光杨华陆健吴殿辉工业生物技术教育部重点实验室江南大学生物工程学院江苏无锡214122 粮食发酵与食品生物制造国家工程研究中心(江南大学) 江苏无锡214122 江苏省生物活性制品加工工程技术研究中心(江南大学) 江苏无锡214122

该研究的目的是选育1株高产酯酶的酿酒酵母,通过增加菠萝蜜酒中酯类的含量提高菠萝蜜酒的香气质量。以菠萝蜜果浆自然发酵液中分离获得的1株产酯酶酿酒酵母CS31为出发菌株,利用常压室温等离子体(atmospheric and room temperature plasm... 详细信息

该研究的目的是选育1株高产酯酶的酿酒酵母,通过增加菠萝蜜酒中酯类的含量提高菠萝蜜酒的香气质量。以菠萝蜜果浆自然发酵液中分离获得的1株产酯酶酿酒酵母CS31为出发菌株,利用常压室温等离子体(atmospheric and room temperature plasma,ARTP)进行诱变育种,通过酒精耐受性实验、杜氏小管产气实验及菠萝蜜果酒发酵性能实验进行初筛,检测菌株对不同碳链长度底物(C2、C4和C6)的酯酶活性进行复筛,选育出1株能顺利完成果酒发酵的高产酯酶突变菌株YB93。随后,通过比较突变菌株YB93和出发菌株发酵菠萝蜜果酒的香气成分,进一步验证该突变菌株的产香能力。结果表明,突变菌株YB93的酯酶(C2~C6)比活力均明显高于出发菌株CS31,其中YB93的最大的酯酶(C2)比活力(952.67 U/g)比CS31(764.32 U/g)高出24.64%。相对于出发菌株,经突变菌株YB93发酵的酒样总挥发性物质提高了34.28%,酯类物质提高了60.67%,有效地提升了果酒中果香和花香类化合物的含量,明显增强了菠萝蜜果酒的香气品质。此外,突变菌株YB93经鉴定为酿酒酵母(Saccharomyces cerevisiae),且5次连续传代性能稳定,具有应用于果酒发酵的潜力。

关键词：菠萝蜜果酒酿酒酵母常压室温等离子诱变酯酶酯类物质

SRSF1通过可变剪接调控小鼠减数分裂期间的同源染色体配对和联会

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Science Bulletin 2023年第11期68卷 1100-1104,M0003页

作者：孙龙杰陈娟叶融吕征陈雪雪谢小梅李雨衡王超凡吕鹏博阎璐田爽姚晓红陈忱崔胜刘佳利 State Key Laboratory of Animal Biotech Breeding College of Biological SciencesChina Agricultural UniversityBeijing 100193China Key Laboratory of Precision Nutrition and Food Quality Department of Nutrition and HealthChina Agricultural UniversityBeijing 100190China Key Laboratory of RNA Biology Institute of BiophysicsChinese Academy of SciencesBeijing 100101China Department of Animal Science Michigan State UniversityEast Lansing 48824USA Reproductive and Developmental Sciences Program Michigan State UniversityEast Lansing 48824USA Department of Obstetrics Gynecology and Reproductive BiologyMichigan State UniversityGrand Rapids 49503USA College of Veterinary Medicine Yangzhou UniversityYangzhou 225009China

同源染色体配对和联会是第一次减数分裂过程中的重要环节.然而,可变剪接(AS)在同源染色体配对和联会中发挥作用的基本机制仍不清楚.本文揭示了SRSF1对于同源染色体配对和联会中的AS和基因表达至关重要.小鼠生精细胞中Srsf1的缺失导致同... 详细信息

同源染色体配对和联会是第一次减数分裂过程中的重要环节.然而,可变剪接(AS)在同源染色体配对和联会中发挥作用的基本机制仍不清楚.本文揭示了SRSF1对于同源染色体配对和联会中的AS和基因表达至关重要.小鼠生精细胞中Srsf1的缺失导致同源染色体配对和联会异常,引发非阻塞性无精子症(NOA).在同源染色体识别、端粒介导的染色体移动以及联会复合体(SC)组装过程中,SRSF1与TRA2B和U2AF2相互作用,直接结合并通过AS调控Dmc1、Sycp1和Sun1的表达.总之,本研究揭示了在同源染色体配对和联会中SRSF1介导的转录后调控的关键作用,为阐明雄性减数分裂转录后调控的分子网络提供理论依据.

关键词：可变剪接同源染色体减数分裂联会复合体分子网络转录后调控基因表达组装过程

煤与瓦斯突出与断层控制作用的研究热点及脉络演进--基于CiteSpace的可视化分析

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华北科技学院学报 2023年第5期20卷 117-124页

作者：胡嘉英陈学习陈星宇华北科技学院矿山安全学院北京东燕郊065201

为了解国内、外关于断层构造对瓦斯突出控制作用的研究概况,以2000~2022年CNKI和Web of Science期刊库为基础数据,利用CiteSpace软件构建知识图谱,梳理煤与瓦斯突出与断层控制作用发展脉络,可视化表达其研究热点。研究表明:结合关键词... 详细信息

为了解国内、外关于断层构造对瓦斯突出控制作用的研究概况,以2000~2022年CNKI和Web of Science期刊库为基础数据,利用CiteSpace软件构建知识图谱,梳理煤与瓦斯突出与断层控制作用发展脉络,可视化表达其研究热点。研究表明:结合关键词进行分析,煤与瓦斯突出发生机理研究主要集中于瓦斯、应力与构造煤方面,且构造煤和瓦斯抽采为高度关注的对象,表征其是当前的研究热点;断层构造-突出发生机理目前主要研究断层带瓦斯和应力特征以及构造煤和构造特征,现场预测防治方面则依赖于主控因素、敏感指标及实时预警方面的探究;在时间分布上,突出机理研究在2000~2015年间快速发展,以构造煤及构造因素的定量分析研究为主;突出预测防治方面有明显发展,防治技术手段更加先进且多样化;断层带突出防治措施方面有较大的进步,出现了更加系统综合的突出防治措施及预测方法。在可视化分析基础上,深入研究煤与瓦斯突出领域前沿热点和发展趋势。

关键词：煤与瓦斯突出断层研究热点 CiteSpace 可视化