Vacuolar Phosphate Transporter1 (VPT1) may transport sugar in response to soluble sugar status of grape fruits

作     者：Qian Bai Xuexue Chen Zhenzhen Zheng Jinjing Feng Yanjun Zhang Yuanyue Shen Yun Huang Qian Bai;Xuexue Chen;Zhenzhen Zheng;Jinjing Feng;Yanjun Zhang;Yuanyue Shen;Yun Huang

作者机构：Beijing Key Laboratory for Agricultural Application and New TechniqueCollege of Plant Science and TechnologyBeijing University of AgricultureBeijing 102206China Ministry of Education Key Laboratory of Silviculture and ConservationCollege of ForestryBeijing Forestry University35 East Qinghua RoadBeijing 100083China 

出 版 物：《Horticulture Research》 (园艺研究（英文）)

年 卷 期：2023年第10卷第2期

页      面：94-105页

核心收录：

学科分类：0710[理学-生物学] 09[农学] 0902[农学-园艺学] 090201[农学-果树学] 

基　　金：supported by the National Natural Science Foundation of China(Projects 32030100,32102362),Natural Science Foundation of Beijing(6222004) National Key Research and Development Program(2018YFD1000200) Science and Technology Innovation Support Program(BUA-HHXD2022005) Research and Innovation Ability Improvement Program for Young Teachers of Beijing University of Agriculture 

主　　题：sugar fir transport 

摘      要：Vacuolar Phosphate Transporter1(VPT1)-mediated phosphate uptake in the vacuoles is essential to plant development and fruit ***,here we find that the VPT1 may transport sugar in response to soluble sugar status of *** VvVPT1 protein isolated from grape(Vitis vinifera)berrieswas tonoplast-localized and contains SPX(Syg1/Pho81/XPR1)and MFS(major facilitator superfamily)*** mRNA expression was significantly increased during fruit ripening and induced by *** analyses based on transient transgenic systems in grape berry showed that VvVPT1 positively regulated berry ripening and significantly affected hexose contents,fruit firmness,and ripening-related gene *** VPT1 proteins(Grape VvVPT1,strawberry FaVPT1,and Arabidopsis AtVPT1)all showed low affinity for phosphate verified in yeast system,while they appear different in sugar transport capacity,consistent with fruit sugar ***,our findings reveal a role for VPT1 in fruit ripening,associated to its SPX and MFS domains in direct transport of soluble sugar available into the vacuole,and open potential avenues for genetic improvement in fleshy fruit.