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DNA Extraction from Eriocaulon Plants and Construction of RAPD System

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Forestry Studies in China 2004年第1期6卷 22-26页

作者： xuexian LinShanzhi ZhangZhixiang KeyLaboratoryforGeneticsandBreedinginForestTreesandOrnamentalPlants MOEBeijingForestryUniversityBeijing100083P.R.China

There have been many arguments on the classification of Eriocaulon Linn. by morphology so far, and little is known about the use of molecular marker for genetic for genetic diversity of Eriocaulon plants. To apply the technique of molecular marker to the research of genetic diversity of Eriocaulon plants, the study of the extraction method of DNA from the Eriocaulon plants and the RAPD system are essential for researchers. In this paper, the extraction of genome DNA from the silica-gel-dried leaves of several species of Eriocaulon distributed in China was studied, and the best RAPD analysis technique condition of Eriocaulon plants was analyzed.

关键词： Eriocaulon DNA extraction RAPD genetic diversity

Studies on the Distribution and Radioimmunoimaging of ^(99m)Tc-Labeled 5-Fluorouracil Loaded Immunological Nanoparticles in Tissues and Human Gastric Carcinoma Xenografts

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Chinese Journal of Clinical Oncology 2007年第5期4卷 307-312页

作者： Kaihong Huang Jianhua Liu Zhaohua Zhu xuexian Li Xianping Lu Institute of Gastroenterology Departmentof Nuclear Medicine the 2nd Affiliated Hospital of Sun Yat-sen University Guangzhou510120 China.

OBJECTIVE To explore the method of preparation of 99mTc labeled AntiVEGF McAb 5-FU loaded polylactic acid nanoparticles(99mTc-5-FU-Ab-NPs), and investigate the biological distribution of the nanoparticles in human gas- tric carcinoma xenografts. METHODS Anti-VEGF monoclonal antibodyes(MCAB)in 5-FU-Ab-NPs were labeled with 99mTc using a modified Schwarz *** isolation of the 99mTc-5-FU-Ab-NPs using a Sephadex G-250 column,the labeling percentage and radiochemical purity were determined using paper chromatog- *** immunocompetence of the 99mTc-5-FU-Ab-NPs as tumor markers was determined using ELISA and immunohistochemistry.99mTc-5-FU-Ab-NPs (experimental group),99mTc-labelled murine multiclonal IgG loaded polylactic acid and nanoparticles(control group)were injected via the tail vein into SCID mice bearing human gastric carcinoma.A radio-immunity ECT image was developed at 2 and 6 h after the *** the ECT imaging, the mice were sacrificed,their tissue and tumor radioactivity distribution determined,and percentage of the injected-dose per gram(%ID/g)and tumor/ nontumor(T/NT)ratio *** performance liquid chromatography (HPLC)was used to determine the 5-FU concentration in the tumor tissue and blood in the mice of both groups. RESULTS The percentage of 99mTc-5-FU-Ab-NPs labeling was 90%~95%.There was no obvious decrease in the antibody activity before and after *** radio-immuno-imaging(RII)showed that the tumor image had developed 2 h after injection of the 99mTc-5-FU-Ab-NPs,and with time it was clearer at the 6th hour fol owing the ***%ID/g of the tumor tissue at both 2 h and 6 h after the injection was significantly higher compared to the control *** tumor%ID/g and the tumor to blood activity ratio(TB)of the experimental group at 6 h following the injection increased compared to that at 2 h,and at the same time,5-FU concentration in the tumor of the experimental group continuously increased over time,and s

关键词：放射性核图象胃癌症单克隆抗体

Spatial Para Allocation of a Small Watershed Protection Forest System

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Chinese Forestry Science and Technology 2012年第3期11卷 35-36页

作者： chen Junhua Gong Gutang Zhu Zhifang Wu xuexian Mu Changlong Sichuan Academy of Forestry

With the interpreted IKONOS4 satellite images collected in 2005,a 1:10 000 forest map,the data collected in ClassⅡforest survey in 2007,and surface runoff data obtained in the permanent runoff monitoring station,six site factors of slope gradient, slope position,soil thickness,soil thickness of A layer,soil type and soil water content were selected to establish a matter element model suitable to 297 forest ***,the AHP was applied to modify the spatial structure and form the spatial para allocation of the protection forest system. The results showed that:1) the area allocation of different vegetation types was irrational,the area of a mixed coniferous and broad-leafed forest was 124.39 ha,accounting for 14.97%of the forested land,the mixed forest was consisted of two coniferous species accounted for 27.37%of the total forest land,and the pure forest was consisted of one coniferous species accounted for 42.48%.2) After optimization,the area allocation of different vegetation types in the protection forest system ranked as:the mixed coniferous and broad-leafed forest(31.12%)>shrub land(15.5%)>the economic tree crops(12.28%)>the mixed forest consisting of two coniferous species(11.46%)>the pure broad-leafed forest(11.41%)>the pure coniferous forest(9.51%)>grass land(8.70%). Besides,the annual surface runoff could reduce 43 171 mm or 62.15%,annual soil erosion could decrease 465.97 t or 79.66%.3) The following suggestions for spatial para allocation of the small watershed protection forest system were proposed: (1) Reducing pure cypress or pine forest,and increasing alder-cypress,oak-cypress or pine-cypress mixed *** for the current cypress and pine pure forests,alder or oak could be introduced to develop a mixed forest.(2) The canopy closure of pure cypress forest and alder-cypress forests should be maintained within 0.60-0.75,and the canopy closure of oak-cypress,pine-cypress or oak-pine forests should be controlled within 0

关键词： matter element analysis analytic hierarchy process Guansi river watershed protection forest spatial para allocation structural optimization

基于分布式振动传感技术的铁路边坡监测系统研究

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湖南交通科技 2023年第3期49卷 201-206页

作者：彭学先湖南湘建智科工程技术有限公司湖南长沙410205

铁路边坡安全预警系统在信息化监测方面取得重要进展,出现了各类传感器及监测云平台。以京广铁路衡阳段某里程一处边坡为试验点,研究分布式振动传感技术在铁路边坡监测系统中的应用。研究表明:竖向垂直铁轨方向的采集值较水平方向更为明... 详细信息

铁路边坡安全预警系统在信息化监测方面取得重要进展,出现了各类传感器及监测云平台。以京广铁路衡阳段某里程一处边坡为试验点,研究分布式振动传感技术在铁路边坡监测系统中的应用。研究表明:竖向垂直铁轨方向的采集值较水平方向更为明显;振动峰值随落石距传感器位置的增大而逐渐较小,当落石与传感器距离达到15 m后,振动峰值变化效果不明显。研究可为振动传感器在铁路边坡安全监测中的应用提供借鉴,为铁路边坡的长期在线安全监测提供了一种新方法。

关键词：铁路边坡安全监测柔性格栅加速度传感器京广铁路

WEIGHTED INEQUALITIES FOR CERTAIN MAXIMAL FUNCTIONS IN ORLICZ SPACES

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Analysis in Theory and Applications 2001年第4期17卷 65-76页

作者： Zhu xuexian (Peking University, China) Peking University China

Let Mg be the maximal operator defined by $$M_g f\left( x \right) = \sup \frac{{\int_a^b {f\left( y \right)g\left( y \right){\text{d}}y} }}{{\int_a^b {g\left( y \right){\text{d}}y} }}$$ , where g is a positive locally integrable function on R and the supremum is taken over all intervals [a,b] such that 0≤a≤x≤b/η(b?a), here η is a non-increasing function such that η (0) = 1 and $\mathop {{\text{lim}}}\limits_{t \to {\text{ + }}\infty } \eta \left( t \right) = 0$ η (t) = 0. This maximal function was introduced by H. Aimar and L. L. Forzani [AF]. Let Φ be an N - function such that Φ and its complementary N - function satisfy Δ2. It gives an A′Φ(g) type characterization for the pairs of weights (u,v) such that the weak type inequality $$u\left( {\left\{ {x \in {\text{R}}\left| {M_g f\left( x \right) >\lambda } \right.} \right\}} \right) \leqslant \frac{C}{{\Phi \left( \lambda \right)}}\int_{\text{R}} {\Phi \left( {\left| f \right|v} \right)} $$ holds for every f in the Orlicz space LΦ(v). And, there are no (nontrivial) weights w for which (w,w) satisfies the condition A′Φ(g).

关键词： ORLICZ SPACES maximal operator maximal function Orlicz space

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基于距离优势关系的高维多目标进化算法

计算机科学与探索 2022年第11期16卷 2642-2652页

作者：顾清华徐青松李学现西安建筑科技大学管理学院西安710001 西安建筑科技大学资源工程学院西安710001 西安市智慧工业感知计算与决策重点实验室西安710001

收敛性与多样性是多目标进化算法的两个主要研究方面,随着多目标优化问题目标维度的增加,传统的多目标进化算法很难维持解的多样性。为了增强算法在高维多目标优化问题中的多样性,提出了一种新的距离优势关系。首先,为了保证算法的收敛... 详细信息

收敛性与多样性是多目标进化算法的两个主要研究方面,随着多目标优化问题目标维度的增加,传统的多目标进化算法很难维持解的多样性。为了增强算法在高维多目标优化问题中的多样性,提出了一种新的距离优势关系。首先,为了保证算法的收敛性,在同一小生境内,基于距离优势关系计算候选解到理想点的距离作为适应度值,选择适应度值好的候选解作为非支配解。然后,为了增强算法的多样性,距离优势关系设定了每个候选解具有相同的小生境,并且保证在同一小生境内只保留一个最优解。最后,基于提出的距离优势关系对VaEA算法进行改进,得到的算法命名为VaEA-DDR。在5、8、10、15维目标的DTLZ及IDTLZ测试问题上将改进的算法与目前六种常用的算法进行实验对比。实验结果表明,改进后的算法具有较强的竞争性,能显著增强算法的多样性。

关键词：高维多目标优化距离优势关系多样性 VaEA

“双高”背景下提升高职教师工作投入的对策分析

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辽宁省交通高等专科学校学报 2022年第5期24卷 29-35页

作者：吴雪贤浙江交通职业技术学院浙江杭州311112

高职院校“双高建设”任务圆满完成的第一要务是广大教师的工作投入。本文基于对高职教师工作投入的现状与影响因素的关系进行分析,提出增强其工作投入的对策建议,以促进高职教育的高质量发展。

关键词：双高背景高职教师工作投入对策分析

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人工智能背景下采矿系统工程发展现状与展望

金属矿山 2022年第5期51卷 10-25页

作者：顾清华江松李学现卢才武陈露西安建筑科技大学资源工程学院陕西西安710055 西安市智慧工业感知计算与决策重点实验室陕西西安710055 西安建筑科技大学管理学院陕西西安710055

随着以人工智能为核心的第四次工业革命的到来,采矿系统工程作为采矿工程与系统工程的交叉学科方向,迎来了前所未有的发展机遇和挑战。首先梳理了采矿系统工程涉及的基本理论和研究方法,在此基础上对人工智能技术在采矿系统工程中的发... 详细信息

随着以人工智能为核心的第四次工业革命的到来,采矿系统工程作为采矿工程与系统工程的交叉学科方向,迎来了前所未有的发展机遇和挑战。首先梳理了采矿系统工程涉及的基本理论和研究方法,在此基础上对人工智能技术在采矿系统工程中的发展历史进行了回顾,通过对研究现状进行分析,综述了当前人工智能、大数据背景下采矿系统工程的研究方法和研究应用对象,最后指出了未来采矿系统工程在跨学科应用、大系统优化及智能采矿方面的发展趋势。研究表明:以进化计算和机器学习为代表的人工智能技术,在新一代新能源智能化无人采矿装备,无人采矿新技术、新工艺及新模式,采矿生产各工艺流程智能化和多系统融合大数据平台等4个重要发展方向中将发挥越来越重要的作用,是未来引领智能矿山建设的核心技术,人工智能技术与传统采矿工艺的结合必将推动采矿系统工程学科进入全新的发展阶段。

关键词：采矿系统工程人工智能智能矿山机器学习多目标算法发展趋势

松辽盆地北部白垩纪青山口组黑色页岩元素地球化学特征及沉积古环境恢复

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地质科学 2022年第1期57卷 156-171页

作者：王岚周海燕商斐周学先中国石油勘探开发研究院北京100083

对松辽盆地北部青山口组118块页岩样品进行有机碳和元素地球化学测试,运用一系列判别古气候、古盐度、古生产力和古氧化还原条件的地化指标,恢复青山口组黑色页岩的沉积环境,探讨富有机质页岩成因机制。青山口组沉积期为温暖半湿润亚热... 详细信息

对松辽盆地北部青山口组118块页岩样品进行有机碳和元素地球化学测试,运用一系列判别古气候、古盐度、古生产力和古氧化还原条件的地化指标,恢复青山口组黑色页岩的沉积环境,探讨富有机质页岩成因机制。青山口组沉积期为温暖半湿润亚热带气候,古气温>15℃,水体为陆相微咸水—半咸水环境,盐度5‰~10‰。青山口组底部发育厚度约30 m的富有机质页岩,TOC最高达到9.39%,自下而上有机碳含量逐渐变低。富有机质页岩Cu、Ni、Mo和Zn含量高,发育大量胶磷矿、藻席,表明青山口组早期湖盆生产力达到峰值,为有机质富集提供物质基础。U元素含量以及V/(V+Ni)、V/Sc比值较高,表明青山口组早期水体为强还原环境,为富有机质页岩保存提供有利条件。中-晚期随着湖盆收缩、水体变浅,还原环境遭到破坏,有机碳含量降低。TOC与古生产力及氧化还原指标呈正相关关系,表明有机质含量的变化主要受控于湖盆生产力和氧化还原环境。古海侵事件带来丰富营养物质,极大提高了湖盆生产力,保证有机质大量供应;同时海侵造成盐度分层,使底部水体的强还原环境不受破坏,促进了有机质的保存。松辽盆地青山口组在白垩纪全球缺氧事件影响下形成了面积约5×10^(-3)km;的黑色页岩,是页岩油勘探的重要目标。

关键词：元素地球化学氧化还原环境海侵白垩纪全球缺氧事件松辽盆地