检索结果-南通市图书馆

Research 2024年第1期2023卷 271-280页

作者： Qing Xia Rui Liu xueqin chen Zixuan chen Jun-Jie Zhu State Key Laboratory of Analytical Chemistry for Life Science School of Chemistry and Chemical EngineeringNanjing UniversityNanjing 210023P.R.China Shenzhen Research Institute of Nanjing University Shenzhen 518000P.R.China

Metal nanomaterials can facilitate microbial extracellular electron transfer(EET)in the electrochemically active biofilm.However,the role of nanomaterials/bacteria interaction in this process is still unclear.Here,we reported the single-cell voltammetric imaging of Shewanella oneidensis MR-1 at the single-cell level to elucidate the metal-enhanced EET mechanism in vivo by the Fermi level-responsive graphene electrode.Quantified oxidation currents of~20 fA were observed from single native cells and gold nanoparticle(AuNP)-coated cells in linear sweep voltammetry analysis.On the contrary,the oxidation potential was reduced by up to 100 mV after AuNP modification.It revealed the mechanism of AuNP-catalyzed direct EET decreasing the oxidation barrier between the outer membrane cytochromes and the electrode.Our method offered a promising strategy to understand the nanomaterials/bacteria interaction and guide the rational construction of EET-related microbial fuel cells.

关键词： Fermi voltammetry Electron

A prognosis model for predicting immunotherapy response of esophageal cancer based on oxidative stress-related signatures

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Oncology Research 2024年第1期32卷 199-212页

作者： JING GUO CHANGYONG TONG JIANGUANG SHI XINJIAN LI xueqin chen Department of Thoracic Surgery The First Affiliated Hospital of Ningbo UniversityNingboChina Department of Chinese Traditional Medicine The First Affiliated Hospital of Ningbo UniversityNingboChina

Oxidative stress(OS)is intimately associated with tumorigenesis and has been considered a potential therapeutic strategy.However,the OS-associated therapeutic target for esophageal squamous cell carcinoma(ESCC)remains unconfirmed.In our study,gene expression data of ESCC and clinical information from public databases were downloaded.Through LASSO-Cox regression analysis,a risk score(RS)signature map of prognosis was constructed and performed external verification with the GSE53625 cohort.The ESTIMATE,xCell,CIBERSORT,TIMER,and ImmuCellAI algorithms were employed to analyze infiltrating immune cells and generate an immune microenvironment(IM).Afterward,functional enrichment analysis clarified the underlying mechanism of the model.Nomogram was utilized for forecasting the survival rate of individual ESCC cases.As a result,we successfully constructed an OS-related genes(OSRGs)model and found that the survival rate of high-risk groups was lower than that of low-risk groups.The AUC of the ROC verified the strong prediction performance of the signal in these two cohorts further.According to independent prognostic analysis,the RS was identified as an independent risk factor for ESCC.The nomogram and follow-up data revealed that the RS possesses favorable predictive value for the prognosis of ESCC patients.qRT-PCR detection demonstrated increased expression of MPC1,COX6C,CYB5R3,CASP7,and CYCS in esophageal cancer patients.In conclusion,we have constructed an OSRGs model for ESCC to predict patients’prognosis,offering a novel insight into the potential application of the OSRGs model in ESCC.

关键词： Esophageal carcinoma OS Prognosis Risk markers Immune cell infiltration

Josephin domain containing 2(JOSD2)promotes lung cancer by inhibiting LKB1(Liver kinase B1)activity

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Signal Transduction and Targeted Therapy 2024年第2期9卷 693-708页

作者： Tao Yuan chenming Zeng Jiawei Liu chenxi Zhao Fujing Ge Yuekang Li Meijia Qian Jiamin Du Weihua Wang Yonghao Li Yue Liu Xiaoyang Dai Jianya Zhou xueqin chen Shenglin Ma Hong Zhu Qiaojun He Bo Yang Institute of Pharmacology&Toxicology Zhejiang Province Key Laboratory of Anti-Cancer Drug ResearchCollege of Pharmaceutical SciencesZhejiang UniversityHangzhou 310058China Innovation Institute for Artificial Intelligence in Medicine Zhejiang UniversityHangzhou 311199China Ministry of Education Key Laboratory of Chinese Medicinal Plants Resource from Lingnan Research Center of Medicinal Plants Resource Science and EngineeringGuangzhou University of Chinese MedicineGuangzhou 510006China Center for Drug Safety Evaluation and Research of Zhejiang University Hangzhou 310058China Department of Respiratory Disease Thoracic Disease CenterThe First Affiliated HospitalCollege of MedicineZhejiang UniversityHangzhou 310006China Department of Oncology Hangzhou Cancer HospitalHangzhou 310002China Cancer Center of Zhejiang University HangzhouChina Engineering Research Center of Innovative Anticancer Drugs Ministry of EducationHangzhouChina

Non-small cell lung cancer(NSCLC)ranks as one of the leading causes of cancer-related deaths worldwide.Despite the prominence and effectiveness of kinase-target therapies in NSCLC treatment,these drugs are suitable for and beneficial to a mere~30%of NSCLC patients.Consequently,the need for novel strategies addressing NSCLC remains pressing.Deubiquitinases(DUBs),a group of diverse enzymes with well-defined catalytic sites that are frequently overactivated in cancers and associated with tumorigenesis and regarded as promising therapeutic targets.Nevertheless,the mechanisms by which DUBs promote NSCLC remain poorly understood.Through a global analysis of the 97 DUBs’contribution to NSCLC survival possibilities using The Cancer Genome Atlas(TCGA)database,we found that high expression of Josephin Domain-containing protein 2(JOSD2)predicted the poor prognosis of patients.Depletion of JOSD2 significantly impeded NSCLC growth in both cell/patient-derived xenografts in vivo.Mechanically,we found that JOSD2 restricts the kinase activity of LKB1,an important tumor suppressor generally inactivated in NSCLC,by removing K6-linked polyubiquitination,an action vital for maintaining the integrity of the LKB1-STRAD-MO25 complex.Notably,we identified the first small-molecule inhibitor of JOSD2,and observed that its pharmacological inhibition significantly arrested NSCLC proliferation in vitro/in vivo.Our findings highlight the vital role of JOSD2 in hindering LKB1 activity,underscoring the therapeutic potential of targeting JOSD2 in NSCLC,especially in those with inactivated LKB1,and presenting its inhibitors as a promising strategy for NSCLC treatment.

关键词： LKB1 inhibiting removing

Succession of bacteria communities during production and application of dairy bedding by membrane-covered aerobic fermentation

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

International Journal of Agricultural and Biological Engineering 2024年第1期17卷 232-240页

作者： Hongjie Yin Jianfei Zeng chen Fang xueqin He Ya Su Jinpeng Xiong Lujia Han Guangqun Huang College of Engineering China Agricultural University Beijing 100083China Institute of Environment and Sustainable Development in Agriculture Chinese Academy of Agricultural SciencesBeijing 100081China College of Agriculture Guizhou UniversityGuiyang 550025China

The cost of dairy manure treatment and bedding material purchase increases the operating cost of the dairy farm.Membrane-covered aerobic fermentation system has been widely used for dealing with dairy manure and recycling the final product as bedding material.However,the microbial safety in each processing step is still uncertain.To better understand the bacterial community dynamics during the whole bedding conversion process,a full-chain and large-scale experiment including 16-day membrane-covered aerobic fermentation and 11-day bedding material application was conducted.The results showed that the pile temperatures in the fermentation stage rapidly increased to 80°C and maintained>50°C for more than 11 days and the use of fermentation product as bedding material provided cows with a stable and comfortable bedding environment.The Chao1 and Shannon index decreased at the end of the fermentation stage and remained stable in the application stage,indicating that membrane-covered aerobic fermentation effectively killed some pathogenic bacteria and guaranteed both the maturity and stability of the final product.The dominant bacteria in the fermentation stage were Acinetobacter,Thermus,and Rhodothermus at genus level.Seven common potential pathogens of mastitis(Staphylococcus,Enterococcus,Serratia,Pseudomonas,Corynebacterium,Mycobacterium,and Bacillus)were found at the end of fermentation stage but the relative abundance was low(0.0025%-0.2727%).The dominant bacteria in the application stage mainly included Acinetobacter,Pseudomonas,and Flavobacterium at the genus level.The relative abundance of Pseudomonas increased in the application stage,which was a reminder to the dairy farm to pay attention to the disinfection and timely replacement of bedding material to prevent the occurrence of dairy mastitis.The results of this study contributed deep understanding of the microorganism-driven bedding conversion process and provide practical guidance and cautions for the bedding materials application.

关键词： semi-permeable membrane dairy manure aerobic fermentation bedding material bacterial diversity

DNA functionalized plasmonic nanoassemblies as SERS sensors for environmental analysis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

聚集体(英文)

聚集体(英文) 2023年第2期4卷 62-82页

作者： Jinxiang Li xueqin chen Jun-Jie Zhu School of Chemistry and Chemical Engineering Nanjing UniversityNanjingPR China

Surface-enhanced Raman scattering(SERS)is among the most widely applied analytical techniques due to its easy execution and extreme sensitivity.Target molecules can be detected and distinguished based upon the fingerprint spectra that arise when absorbed on the SERS substrates surface,particularly on the SERS-active hotspots.Thus,rational fabricating the enhancing substrates plays a key role in broadening SERS application.Programmable DNA functionalized plasmonic nanoassemblies,where DNA acts as both structure basis and functional unit,combine the specificity of DNA recognition,and modulate the assembly of plasmonic nanoparticles(NPs).Specifically designed DNA not only improves the selectivity to target molecules but also promotes the sensitivity of the optical signals through precisely regulating the distance between the molecule and the substrate.A variety of DNA-functionalized SERS sensors have been reported and obtained well performance in the analysis of heavy metal ions in water,toxins,pesticide residues,antibiotics,hormones,illicit drugs,or other small molecules.This review places an emphasis on the design and sensing strategies of the DNA-functionalized plasmonic nanoassemblies,as well as basic principles of Raman enhancement,and recent advances for environmental analysis.The current challenges and potential trends in the development of DNAfunctionalized SERS sensors for environmental pollutant monitoring in complicated scenarios are subsequently discussed.

关键词： assemblies DNA environmental analysis nanostructures SERS

Zokor activity promotes soil water infiltration in the Mu Us sandy land of northern Shaanxi,China

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Pedosphere 2024年第1期34卷 136-145页

作者： Miao GAN Xuchao ZHU xueqin YANG Xiaorong WEI Qingyin ZHANG Tongchuan LI Ming'an SHAO Meixia MI Xi YANG Mingyu chen State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau Northwest A&F UniversityYangling 712100China College of Natural Resources and Environment Northwest A&F UniversityYangling 712100China State Key Laboratory of Soil and Sustainable Agriculture Institute of Soil ScienceChinese Academy of SciencesNanjing 210008China Henan Institute of Science and Technology Xinxiang 453003China College of Urban and Rural Construction Shanxi Agricultural UniversityJinzhong 030801China

Zokors are common subterranean rodents that inhabit agricultural fields, shrublands, and grasslands in the arid and semi-arid regions of China. Zokor burrowing activities can alter soil structure and affect soil hydrological processes;however, there are few studies regarding their effects on soil preferential flow in the Mu Us sandy land. An evaluation of the effects of zokor disturbance on their habitat and soil water is important for understanding the ecological role of zokors in the soil ecosystem of the Mu Us sandy land. A field dye-tracing experiment was conducted in the Gechougou watershed on the southeastern edge of the Mu Us sandy land to investigate the effect of zokor burrowing activity on soil preferential flow characteristics. Our results showed that the density of zokor tunnels was the highest(0.40–0.46 m m^(-2)) under 30%–50% vegetation coverage and that the tunnels were approximately 3 cm from the surface.Both stained area ratio and stained path number were higher at sites with zokors than without zokors. Stained path widths were 10–80 and > 80 mm at zokor-harboring sites exhibiting homogeneous flow and heterogeneous finger flow, respectively. In the absence of zokors, homogeneous flow and highly interacted macropore flow were predominant. Soil water content below the zokor tunnels was higher than that above the tunnels. Moderate disturbance of soil structure by zokor activity facilitated soil water infiltration. These results enabled a better understanding of the effect of soil fauna on soil structure and hydrological processes and provided recommendations for ecological construction and renovation in arid and semi-arid regions.

关键词： eld dye-tracing experiment preferential flow soil fauna stained area ratio stained path number stained path width subterranean rodent surface tortoise crack

A grasp planning algorithm under uneven contact point distribution scenario for space non-cooperative target capture

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Aeronautics 2023年第11期36卷 452-464页

作者： Bicheng CAI chengfei YUE Fan WU xueqin chen Yunhai GENG Research Center of the Satellite Technology Harbin Institute of TechnologyHarbin 150001China Institute of Space Science and Applied Technology Harbin Institute of Technology ShenzhenShenzhen 518055China

The contact point configuration should be carefully chosen to ensure a stable capture,especially for the non-cooperative target capture mission using multi-armed spacecraft.In this work scenario,the contact points on the base and on the arms are distributed on the opposite side of the target.Otherwise,large forces will be needed.To cope with this problem,an uneven-oriented distribution union criterion is proposed.The union criterion contains a virtual symmetrical criterion and a geometry criterion.The virtual symmetrical contact point criterion is derived from the proof of the force closure principle using computational geometry to ensure a stable grasp,and the geometry criterion is calculated by the volume of the minimum polyhedron formed by the contact points to get a wide-range distribution.To further accelerate the optimization rate and enhance the global search ability,a line array modeling method and a continuous-discrete global search algorithm are proposed.The line array modeling method reduces the workload of calculating the descent direction and the gradient available,while the continuous-discrete global search algorithm reducing the optimization dimension.Then a highly efficient grasping is achieved and the corresponding contact point is calculated.Finally,an exhaustive verification is conducted to numerically analyze the disturbance resistance ability,and simulation results demonstrate the effectiveness of the proposed algorithms.

关键词： Grasp planning Line array modeling Space debris removal Space non-cooperative target capture Virtual symmetrical contact point method

Numerical simulation of mesoscopic cracking of cement-treated base material based on random polygon aggregate model

在线全文

学校读者我要写书评

暂无评论

Journal of Traffic and Transportation Engineering(English Edition) 2023年第3期10卷 454-468页

作者： Qiao Dong Xiaokang Zhao xueqin chen Jiawei Yuan Wei Hu Ahmad Jahanzaib School of Transportation Southeast UniversityNanjing 211189China National Demonstration Center for Experimental Road and Traffic Engineering Education Nanjing 211189China Department of Civil Engineering Nanjing University of Science and TechnologyNanjing 210094China Biotechnology Engineeringand Skilled Technologies DivisionWake Technical Community CollegeRaleighNC 27603USA

Cracking failure of cement-treated base(CTB)has always been the concern of highway constructors.Mesoscale cracking analysis is an important means to study the damage degradation mechanism,which is difficult to be characterized by experimental techniques alone.The objective of this paper is to develop a random aggregate modelling method to simulate the mesoscopic cracking of CTB material.A minimum rectangle area method was proposed to calculate the polygon aggregate size,which is closer to the sieving analysis than the average radius method.A buffer zone method was proposed to determine the distance between randomly generated polygon aggregates.Based on the proposed random algorithm,finite element method(FEM)was adopted to build the mesoscopic model of CTB including aggregate,mortar,interfacial transition zone(ITZ)and air voids.Laboratory tests were conducted to validate the numerical model.Then the sensitivity analyses were conducted to study the influencing factors on cracking behavior.The simulation results indicate that the higher aggregate content and the finer gradation lead to the increase of ITZ,thus reducing the cracking resistance of the CTB material.Low porosity content is able to significantly reduce the stress concentration and thus improves the cracking resistance.The research results of this paper could be used to guide the crack resistant design of CTB material.

关键词： Cement-treated base Random aggregate Mesoscopic structure Sensitivity analysis Finite element method

Visible Light-Driven Catalyst-Free Amination of Indoles Initiated by Electron Donor-Acceptor Complexes

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Chemistry 2023年第12期41卷 1485-1490页

作者： Jiahong Sui Zhonglie Yang Shuangqiao Li xueqin chen Xiaobin Zhang Qing Shen Hezhong Jiang Jiahong Li School of Life Science and Engineering Sichuan Engineering Research Center for Biomimetic Synthesis of Natural Drugs Southwest Jiaotong University Chengdu Sichuan 610031 China Department of Pharmacy Sichuan Academy of Medical Science & Sichuan Provincial People's Hospital/Personalized Drug Therapy Key Laboratory of Sichuan Province School of Medicine University of Electronic Science and Technology of China Chengdu Sichuan 610072 China

The visible light-driven C2 or C3 amination of indoles without any additives was initiated via electron donor-acceptor (EDA) complex formed by indole and N-aminopyridinium salt.This method was compatible with a wide range of substrates and could proceed smoothly without the addition of any photocatalysts,transition-metal catalysts,or bases.A variety of studies were carried out to examine the presence of EDA complex.

关键词： Photochemistry Amination C-N bond formation Green chemistry N-Heterocycles Donor-acceptor systems

Review on the mesoscale characterization of cement-stabilized macadam materials

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Road Engineering 2023年第1期3卷 71-86页

作者： Qiao Dong Shiao Yan xueqin chen Shi Dong Xiaokang Zhao Pawel Polaczyk Department of Roadway Engineering School of TransportationSoutheast UniversityNanjing 211189China National Demonstration Center for Experimental Road and Traffic Engineering Education Southeast UniversityNanjing 211189China Department of Civil Engineering Nanjing University of Science&TechnologyNanjing 210094China College of Transportation Engineering Chang'an UniversityXi'an 710064China School of Highway Chang'an UniversityXi'an 710064China Department of Civil and Environmental Engineering University of TennesseeKnoxvilleTN 37996USA

The base layer constructed by cement-stabilized macadam(CSM)has been widely used in highway construction due to its low elasticity deformation and high carrying capacity.As a bearing layer,the CSM base is not exempt from fatigue cracking under cyclic loading in the service process.Cracks in the base will create irreversible structural and functional deficiencies,such as the potential for reflective cracking of subsequently placed asphalt concrete overlays.The fracture of the base will shorten the service life of the pavement.The quality of the CSM base is directly related to the bearing capacity and integrity of the whole pavement structure.It is of practical significance to further study the fatigue failure behavior of CSM material for the long-term performance of the pavement.The CSM material is a typical heterogeneous multiphase composite.On the mesoscale,CSM consists of aggregate,cement mortar,pores,and the interface transitional zone(ITZ).On the microscale,the hardened mortar contains a large number of capillary pores,unhydrated particles,hydrated crystals,etc.,which makes the spatial distribution of its material properties stochastic.In addition,cement hydration,dry shrinkage,and temperature shrinkage can also produce micro-crack defects in cement mortar.These microcracks will have crossscale evolution under load,resulting in structural fracture.Macroscopic complex deformation and mechanical response are the reflections of its microscopic and even mesoscale composition and structure.This study summarized the existing studies on the mesoscopic properties of CSM materials,respectively from the three aspects of mesostructure,structural characterization,and mesoscale fatigue damage analysis,to help the development of long-life pavement.The future research direction is to explore the mesoscale characteristics of CSM using multiscale representation and analysis methods,to establish the connection between mesoscale characteristics and macroscopic mechanical properties.

关键词： Road engineering Cement-stabilized macadam Fatigue cracking Mesoscale simulation