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Signal Transduction and Targeted Therapy 2021年第1期6卷 5-7页

作者： Qing Xiong Cheng Peng Xiaomin yan xueqi yan Lin Chen Beicheng Sun Shiping Jiao Department of Hepatobiliary Surgery Nanjing Drum Tower HospitalThe Affiliated Hospital of Nanjing University Medical SchoolNanjing 210093China Drum Tower Research Institute of Clinical Medicine Nanjing UniversityNanjing 210093China Department of Infectious Disease Nanjing Drum Tower HospitalThe Affiliated Hospital of Nanjing University Medical SchoolNanjing 210093China

Dear Editor,The SARS-CoV-2 virus outbreak that started in December 2019 is now a global pandemic with over 42.7 million confirmed cases and 1.15 million deaths as of October 25,*** has been predicted that resurgence in contagion may be possible as late as 2024.1 A persistent anti-SARS-CoV-2 adaptive immune surveillance post infection is critical to lower the risk of resurgence and transmission.T cells are the primary effectors of cellular adaptive immunity that not only clear virus-infected cells during infection but also provide long-term protection post infection as memory cells in some pathogen ***,little is known regarding the longitudinal T cell response during and after SARSCoV-2 ***-cell T cell receptor(TCR)sequencing allows rapid identification of expanded T cell clones that are potentially the antigen-specific clonotypes.

关键词： immunity infection cytotoxic

Cellular atlases of ovarian microenvironment alterations by diet and genetically-induced obesity

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Science China(Life Sciences) 2024年第1期67卷 51-66页

作者： Yonghui Jiang Xueying Gao Yue Liu xueqi yan Huangcong Shi Rusong Zhao Zi-Jiang Chen Fei Gao Han Zhao Shigang Zhao Center for Reproductive Medicine Shandong UniversityJinan 250012China Research Unit of Gametogenesis and Health of ART-Offspring Chinese Academy of Medical Sciences(No.2021RU001)Jinan 250012China Key Laboratory of Reproductive Endocrinology of Ministry of Education Shandong UniversityJinan 250012China Shandong Key Laboratory of Reproductive Medicine Shandong Provincial Hospital Affiliated to Shandong First Medical UniversityJinan 250012China Shandong Provincial Clinical Research Center for Reproductive Health Jinan 250012China National Research Center of Assisted Reproductive Technology and Reproductive Genetics Shandong UniversityJinan 250012China The Affiliated Suzhou Hospital of Nanjing Medical University Suzhou Municipal HospitalGusu SchoolNanjing Medical UniversitySuzhou 215000China Center for reproductive medicine Ren Ji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200127China Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics Shanghai 200127China State Key Laboratory of Stem Cell and Reproductive Biology Institute of ZoologyChinese Academy of ScienceBeijing 100101China

Obesity,which can arise from genetic or environmental factors,has been shown to cause serious damages to the reproductive *** ovary,as one of the primary regulators of female fertility,is a complex organ comprised of heterogeneous cell types that work together to maintain a normal ovarian microenvironment(OME).Despite its importance,the effect of obesity on the entire ovary remains poorly *** this study,we performed ovary single-cell and nanoscale spatial RNA sequencing to investigate how the OME changed under different kinds of obesity,including high-fat diet(HFD)induced obesity and Leptin ablation induced obesity(OB).Our results demonstrate that OB,but not HFD,dramatically altered the proportion of ovarian granulosa cells,theca-interstitial cells,luteal cells,and endothelial ***,based on the spatial dynamics of follicular development,we defined four subpopulations of granulosa cell and found that obesity drastically disrupted the differentiation of mural granulosa cells from small to large antral ***,HFD enhanced follicle-stimulating hormone(FSH)sensitivity and hormone conversion,while OB caused decreased sensitivity,inadequate steroid hormone conversion,and impaired follicular *** differences can be explained by the differential expression pattern of the transcription factor ***,our study provides a powerful and high-resolution resource for profiling obesity-induced OME and offers insights into the diverse effects of obesity on female reproductive disorders.

关键词： obesity ovarian microenvironment snRNA-seq Stereo-seq Foxo1

Inetetamab combined with pyrotinib and oral vinorelbine for patients with human epidermal growth factor receptor 2 positive advanced breast cancer:A single-arm phase 2 clinical trial

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Cancer Pathogenesis and Therapy 2024年第1期2卷 31-37页

作者： Nan Jin Yi Xu Siqi Wang Chunxiao Sun xueqi yan Fan yang yan Liang Weiwei Chen Xiang Huang Department of Oncology The First Affiliated Hospital of Nanjing Medical UniversityNanjingJiangsu 210029China Department of Pathology The First Affiliated Hospital of Nanjing Medical UniversityNanjingJiangsu 210029China Radiology Department The First Affiliated Hospital of Nanjing Medical UniversityNanjingJiangsu 210029China Department of Oncology Affiliated Jinling HospitalMedical School of Nanjing UniversityNanjingJiangsu 210000China

Background Human epidermal growth factor receptor 2(HER2)-targeted agents have significantly improved the outcomes of patients with HER2-positive breast cancer;however,a large proportion of patients still develop resistance to *** this study,we investigated the efficacy and safety of inetetamab,another anti-HER2 antibody,combined with pyrotinib and oral vinorelbine in patients with HER2-positive advanced breast cancer so as to provide new ideas for the *** In this prospective,single-arm,phase 2 trial,patients with HER2-positive advanced breast cancer with disease progression after trastuzumab were *** received a combination of inetetamab(loading dose of 8 mg/kg and subsequent doses of 6 mg/kg intravenously once every 3 weeks),pyrotinib(400 mg orally once daily),and vinorelbine(60 mg/m^(2)orally once weekly)until disease progression or intolerable *** primary endpoint was progression-free survival(PFS).The secondary endpoints included objective response rate(ORR),overall survival(OS),disease control rate(DCR),and *** Between February 13,2022 and December 25,2022,30 patients were screened and enrolled in this *** median age of the patients at enrollment was 54 years,12 patients(40.0%)had hormone-receptor-positive disease and 23 patients(76.7%)had visceral *** median PFS was 8.63 months(95%confidence interval[CI]4.15-13.12 months).The median OS was not *** ORR was 53.3%(16/30)and the DCR was 96.7%(29/30).The most common Grade III/IV adverse events were leukopenia(n=5,16.7%),neutropenia(n=4,13.3%),and diarrhea(n=3,10%).No treatment-related serious adverse events or deaths *** The combination regimen of inetetamab,pyrotinib,and oral vinorelbine showed encouraging efficacy and favorable safety in patients with HER2-positive advanced breast cancer and could be considered as an alternative treatment option for the patients.

关键词： Human epidermal growth factor receptor 2 Breast cancer Inetetamab Pyrotinib Vinorelbine

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晚期乳腺癌内分泌分层治疗进展

实用肿瘤杂志 2023年第2期38卷 105-109页

作者：严雪绮黄香李薇殷咏梅南京医科大学第一附属医院肿瘤科江苏南京210029

晚期激素受体阳性乳腺癌的内分泌治疗已进入靶向时代。周期蛋白依赖性激酶4/6(cyclin-dependent kinase 4/6,CDK4/6)抑制剂、组蛋白去乙酰化酶(histone deacetylase,HDAC)抑制剂和磷脂酰肌醇3-激酶(phosphatidylinositide 3-kinase,PI3K)/蛋白激酶B (protein kinase B,PKB)/哺乳动物雷帕霉素靶蛋白(mammalian target of rapamycin,mTOR)抑制剂等靶向药物的应用已改变了晚期激素受体阳性乳腺癌内分泌治疗的临床实践,显著延长了患者的生存期。面对诸多新型药物,如何制定最优化和个体化的治疗方案仍是临床关注的焦点。2022年新版中国临床肿瘤学会(Chinese Society of Clinical Oncology,CSCO)乳腺癌指南充分考量循证医学证据等级、治疗药物可及性及最新研究进展,并针对既往用药情况提出分层策略,对晚期激素受体阳性乳腺癌的内分泌治疗方案予以相应推荐,为乳腺癌临床实践提供了指导依据。

关键词：乳腺癌内分泌治疗靶向治疗

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急性心肌梗死患者重症护理的方法及效果

现代医药卫生 2015年第5期31卷 671-672,675页

作者：宋兴惠严学棋巴南区人民医院心内科重庆401320

目的探究急性心肌梗死患者重症护理方法和效果。方法选择2010年12月至2012年12月在该院接受治疗的130例急性心肌梗死重症患者,将其随机分成对照组和观察组各65例,对照组给予常规护理,观察组给予护理干预,对比2种护理方法的效果。结果护... 详细信息

目的探究急性心肌梗死患者重症护理方法和效果。方法选择2010年12月至2012年12月在该院接受治疗的130例急性心肌梗死重症患者,将其随机分成对照组和观察组各65例,对照组给予常规护理,观察组给予护理干预,对比2种护理方法的效果。结果护理后,两组患者焦虑评分均降低,但观察组焦虑改善程度明显优于对照组,差异有统计学意义(P<0.05)。观察组患者遵医嘱行为、依从性明显高于对照组,差异有统计学意义(P<0.05)。观察组患者满意度[80.0%(52/65)]明显高于对照组[96.9%(63/65)],差异有统计学意义(P<0.05)。观察组患者住院时间[(4.4±2.6)d]、并发症发生率[1.5%(1/65)]均优于对照组[(8.4±2.5)d、10.8%(7/65)],差异均有统计学意义(P<0.05)。结论在急性心肌梗死重症患者中实施护理干预,有利于提高患者的依从性,改善患者的生活质量。

关键词：心肌梗死危重病护理焦虑病人满意度护理方法学研究

小鼠CCL1基因双荧光素酶报告质粒构建及其与miR-21a-5p靶向关系验证

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黑龙江畜牧兽医 2023年第19期 17-21页

作者：闫雪琪陈明杰齐亚银任静静石河子大学动物科技学院新疆石河子832000

为了验证miR-21a-5p与CCL1基因的靶向关系,试验利用生物信息学软件预测miR-21a-5p和CCL1基因的结合位点,设计引物合成CCL1基因3′非翻译区(UTR)野生型及突变型目的片段,并将其克隆到psiCHECK2质粒上构建CCL1野生型(CCL1-WT)和突变型(CCL... 详细信息

为了验证miR-21a-5p与CCL1基因的靶向关系,试验利用生物信息学软件预测miR-21a-5p和CCL1基因的结合位点,设计引物合成CCL1基因3′非翻译区(UTR)野生型及突变型目的片段,并将其克隆到psiCHECK2质粒上构建CCL1野生型(CCL1-WT)和突变型(CCL1-MUT)双荧光素酶报告质粒。将构建好的CCL1-WT和CCL1-MUT分别与miR-21a-5p模拟物(miR-21a-5p mimic)和阴性对照(miR-NC)共同转染到293T细胞中,检测CCL1-WT+miR-NC组、CCL1-WT+miR-21a-5p mimic组、CCL1-MUT+miR-NC组、CCL1-MUT+miR-21a-5p mimic组的荧光素酶活性。结果表明:CCL1基因是miR-21a-5p的潜在靶基因;CCL1野生型和突变型双荧光素酶报告质粒测序结果与预期结果一致;与CCL1-WT+miR-NC组相比,CCL1-WT+miR-21a-5p mimic组荧光素酶活性显著降低(P0.05)。说明CCL1基因野生型及突变型双荧光素酶报告质粒构建成功,且miR-21a-5p与CCL1基因存在靶向关系。

关键词： miR-21a-5p CCL1基因双荧光素酶报告脑膜炎靶向关系

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新型聚硅铁锌的混凝性能及应用

常州大学学报（自然科学版） 2017年第2期29卷 17-22页

作者：许霞严雪琦王利平常州大学环境与安全工程学院江苏常州213164

以水玻璃、硫酸亚铁、硫酸锌及氯酸钠为原料,用共聚法制备聚硅铁锌混凝剂(PSFZn),观察PSFZn的形态、结构、Zeta电位及混凝性能,并对其混凝机理进行初步探讨。结果表明:n(Fe)/n(Zn)=4的PSFZn(PSFZn4)所呈现的团簇及片层结构能增大其比表... 详细信息

以水玻璃、硫酸亚铁、硫酸锌及氯酸钠为原料,用共聚法制备聚硅铁锌混凝剂(PSFZn),观察PSFZn的形态、结构、Zeta电位及混凝性能,并对其混凝机理进行初步探讨。结果表明:n(Fe)/n(Zn)=4的PSFZn(PSFZn4)所呈现的团簇及片层结构能增大其比表面积,从而增强其混凝性能;同时聚硅铁锌中的Si和Fe、Zn打破了铁盐、锌盐和聚硅酸本身的聚合成键模式,Si-O-Si、Fe-O-Fe、Zn-O-Zn形成了Si-O-Fe、Si-O-Zn键;此外,PSFZn4在p H0,该阶段PSFZn4以电中和、脱稳为主要混凝作用;当p H>3.14时ζ<0,且随p H的进一步增大其负电性就越强,此时PSFZn4主要以吸附架桥及网捕作用为主。通过对实际水样的去除效果对比,发现PSFZn4要远优于聚硅铁(PSF)、聚合硫酸铁(PFS)和聚合硫酸铁铝(PFA)。

关键词：聚硅铁锌(PSFZn) 混凝性能无机高分子

Blog Post Extraction Using Title Finding

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Blog Post Extraction Using Title Finding

粪肠球菌感染小鼠的脑组织病理学观察及转录组学差异分析

第五届全国信息检索学术会议

作者： Linhai Song~(1,2),xueqi Cheng~1,yan Guo~1,Bo Wu~(1,2),Yu Wang~(1,2+) 1 Institute of Computing Technology,Chinese Academy of Sciences,Beijing 2 Graduate School of the Chinese Academy of Sciences,Beijing

With the development of Web2.0,web mining applications pay more attention to blog *** order to prevent noises in blog pages from affecting the precision of web mining algorithms,it is very necessary to acquire posts from biog pages *** this paper,we propose a blog post extraction algorithm which uses title *** are two stages in the *** the first stage,text nodes which indicate the title of the post are found and used as the beginning of the *** take a machine learning approach to realize this stage,and employ SVM as classification *** the second stage,we find the end of the *** methods are introduced in this stage,one uses VIPS segmentation results,and the other is based on hand-coded rules. Experiments are conducted to see how titles are found and how posts are *** results show that our algorithm can obtain promising results.

关键词： Blog Post Title Finding VIPS SVM

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畜牧兽医学报 2022年第9期53卷 3160-3171页

作者：曹梦园陈明杰王晨豫李益涛闫雪琪陈杰齐亚银石河子大学动物科技学院石河子832000

旨在通过构建小鼠感染粪肠球菌后的脑部损伤模型,对不同感染时期脑组织的病理学观察及转录组学差异分析,探索粪肠球菌引起脑组织损伤的机制。本试验选择1株致脑膜炎粪肠球菌,以小鼠为感染动物,感染粪肠球菌后,分别在2、4、6、12、24、36... 详细信息

旨在通过构建小鼠感染粪肠球菌后的脑部损伤模型,对不同感染时期脑组织的病理学观察及转录组学差异分析,探索粪肠球菌引起脑组织损伤的机制。本试验选择1株致脑膜炎粪肠球菌,以小鼠为感染动物,感染粪肠球菌后,分别在2、4、6、12、24、36、60和72 h采集小鼠脑组织,观察脑组织的损伤程度,挑选早期、明显期和转归期3个时间点,通过转录组学测序,对差异表达基因进行分析,使用荧光定量PCR对转录组学数据进行验证。结果显示:小鼠感染粪肠球菌12 h后,脑组织开始出现病变,通过病理组织学观察发现小鼠脑组织先后出现了脑膜及脑组织血管充血,血管周围间隙增宽,脑组织因水肿有网格状间隙,微血栓形成及炎性细胞浸润。对转录组学差异基因分析,GO富集结果显示主要富集到对β干扰素的反应、细胞对β干扰素的反应、对其他生物的防御反应、对γ干扰素的反应、对细菌的反应、外在凋亡信号通路、调节外在凋亡信号通路、神经元凋亡过程、内皮细胞迁移等生物进程中。KEGG富集结果显示差异信号通路主要富集在过氧物酶体、NOD样受体信号通路、氧化磷酸化、钙代谢信号传导途径、PI3K-Akt信号传导途径、Wnt信号通路、MAPK信号通路、GnRH分泌、VEGF信号通路、紧密连接等一些与脑组织损伤相关的通路上。粪肠球菌感染小鼠后,影响脑组织过氧物酶体、NOD样受体信号通路、氧化磷酸化、钙代谢信号传导途径、PI3K-Akt信号传导途径等通路改变,这些通路与蛋白磷酸化、炎症的发生及血脑屏障通透性的改变有关,为进一步研究粪肠球菌引起脑组织损伤机制提供研究方向和理论基础。

关键词：粪肠球菌小鼠脑膜炎病理学差异基因

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直肠癌术前放化疗的敏感性预测及临床应用

国际肿瘤学杂志 2017年第12期44卷 944-947页

作者：严雪绮周俊东吴锦昌南京医科大学附属苏州医院放疗科 215000

部分直肠癌患者对术前同步放化疗（CCRT）敏感性较差，对CCRT治疗抵抗的患者，肿瘤的局部控制效果差，且CCRT可能增加不良反应。临床上通过应用核磁共振、正电子发射断层摄影技术、血清癌胚抗原、分子标志物和基因检测等方法，在治疗前... 详细信息

部分直肠癌患者对术前同步放化疗（CCRT）敏感性较差，对CCRT治疗抵抗的患者，肿瘤的局部控制效果差，且CCRT可能增加不良反应。临床上通过应用核磁共振、正电子发射断层摄影技术、血清癌胚抗原、分子标志物和基因检测等方法，在治疗前对直肠癌患者CCRT的敏感性进行预测。根据预测结果指导临床医生为患者选择个体化治疗方式，从而使直肠癌的治疗效果得到进一步的提高。

关键词：直肠肿瘤术前同步放化疗分子标志物