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Journal of Genetics and Genomics 2015年第9期42卷 507-510页

作者： Zhou Tan Zhongkai Hu Emily Y.Cai Cantas Alev Ting Yang Zhen Li Joyce Sung Yasser Yehia El-Sayed Gary M.Shaw David K.Stevenson Atul J.butte Guojun Sheng Karl G.Sylvester Harvey J.Cohen xuefeng b.ling Institute of Developmental and Regenerative biology Hangzhou Normal University Department of Surgery Stanford University Lab for Early Embryogenesis RIKEN Center for Developmental Biology Department of Obstetrics and Gynecology Stanford University Department of Pediatrics Stanford University Stanford

Over 11% of all pregnancies in the US result in preterm birth, greatly contributing to perinatal morbidity and mortality （Goldenberg and Rouse, 1998）. Preterm birth etiologies remain largely unknown, and effective prevention methods have yet to be developed. The use of biofluid （e.g., serum or urine） for the analysis of the naturally occurring peptidome （MW 〈 4000） as a source of biomarkers has been reported for different diseases （Villanueva et al., 2006; ling et al., 2010a, 2010b, 2010c, 2011）. Mass spectrometry-based profiling of naturally occurring peptides can provide an extensive in- ventory of serum peptides derived from either high-abundant endogenous circulating proteins or cell and tissue proteins （Liotta and Petricoin, 2006）.

关键词： Serological Targeted Analysis of an ITIH4 Peptide Isoform A Preterm birth biomarker and Its Associated SNP Implications SNP

Nitrogen isotope stratigraphy of the Early Cambrian successions in the Tarim basin:Spatial variability of nitrogen cycling and its implication for paleo-oceanic redox conditions

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Acta Geochimica 2024年第4期43卷 785-801页

作者： bi Zhu xuefeng Li Lu Ge Yongquan Chen Institute of Earth’s Critical Zone School of Earth Sciences and EngineeringHohai UniversityNanjing 210098China Research Institute of Exploration and Development Tarim Oil Field CompanyPetroChinaKorlaChina

The Early Cambrian represents a critical time period characterized by extraordinary biological innovations and dynamic redox conditions in *** isotopic signatures of ancient sediments have the potential to elucidate the evolutionary path of marine redox states and the biogeochemical nitrogen cycle within the water column of the Early Cambrian *** existing research on this topic has predominantly focused on South China,the exploration of other continental margins has been limited,leaving contradictory hypotheses *** this study,pairedδ^(15)N andδ^(13)C org analyses were performed on the Lower Cambrian successions from the Shiairike section(inner ramp)and Well Tadong 2(deep shelf/basin)in the northwestern and eastern Tarim basin,*** data from the Shiairike section reveal a discernible shift in the operation of different nitrogen cycles for the black chert-shale unit,also referred to as the black rock series in Chinese literature,of the Yurtus Formation(Fortunian stage to lower Stage 3).Oscillatingδ^(15)N values for its lower part are suggestive of alternating anaerobic assimilation of NH 4+and denitrification/*** is likely attributed to a shallow,unstable chemocline consistent with the upwelling and incursion of deep,anoxic waters during a major *** contrast,aerobic nitrogen cycling,indicated by positiveδ^(15)N values of>2‰,dominated the upper part alongside a reduction in upwelling *** the other hand,theδ^(15)N signatures of Xishanbulake and Xidashan Formations of Well Tadong 2,which encompass a time interval from the Cambrian Fortunian Age to Age 4,are indicative of N_(2)fixation by diazotrophs as the major nitrogen *** two studied intervals,although not time-equivalent,exhibit separated states of nitrogen cycling at least during the deposition of the Yurtus black rock *** spatially different nitrogen cycling of the studied sections is compatible with a redox-stratified ocean dur

关键词： Nitrogen isotopes Early Cambrian Tarim black rock series

An inverse method for characterization of dynamic response of 2D structures under stochastic conditions

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Chinese Journal of Aeronautics 2024年第3期37卷 440-455页

作者： xuefeng LI Abdelmalek ZINE Mohamed ICHCHOU Noureddine bOUHADDI Pascal FOSSAT Laboratory of Tribology and Systems Dynamic Central School of LyonEcully 69134France Institut Camille Jordan Central School of LyonEcully 69134France Department of Applied Mechanics University of Burgundy Franche-ComtéBesancon 25000France

The reliable estimation of the wavenumber space(k-space)of the plates remains a longterm concern for acoustic modeling and structural dynamic behavior *** current analyses of wavenumber identification methods are based on the deterministic *** this end,an inverse method is proposed for identifying wave propagation characteristics of twodimensional structures under stochastic conditions,such as wavenumber space,dispersion curves,and band *** proposed method is developed based on an algebraic identification scheme in the polar coordinate system framework,thus named Algebraic K-Space Identification(AKSI)***,a model order estimation strategy and a wavenumber filter are proposed to ensure that AKSI is successfully *** main benefit of AKSI is that it is a reliable and fast method under four stochastic conditions:(A)High level of signal noise;(b)Small perturbation caused by uncertainties in measurement points’coordinates;(C)Non-periodic sampling;(D)Unknown structural *** validate the proposed method,we numerically benchmark AKSI and three other inverse methods to extract dispersion curves on three plates under stochastic *** experiment is then performed on an isotropic steel *** investigations demonstrate that AKSI is a good in-situ k-space estimator under stochastic conditions.

关键词： Inverse method Dispersion relation Wavenumber space Periodic plates Stochastic conditions Wave propagation characterization

Sensitive and Specific Y-Shaped Ratio biosensor for Detecting Serum mi R-18a:Potential Early Scanning Tool for Non-Small Cell Lung Cancer

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Journal of Analysis and Testing 2024年第2期8卷 237-244页

作者： Fei Yu Yue Wang Caiyuan Yu Wei Zhang xuefeng bai State Key Laboratory of Quality Research in Chinese Medicines Macao Institute for Applied Research in Medicine and HealthMacao University of Science and TechnologyTaipaMacao999078China baotou Cancer Hospital Baotou014040Inner MongoliaChina

miR-18a has been identified as a significantly expressed microRNA(miRNA)in non-small cell lung cancer(NSCLC)and plays a vital role in cancer cell transformation,metastasis,and ***,a pair of binary probes from numerous probe pairs based on single nucleotide polymorphism analyses of miR-18a and miR-18b was first designed and screened to develop a Y-shaped ratio biosensor for accurate detection of serum miR-18a in *** special structure of the binary probes combined with the hairpin showed strong specificity for miR-18a,which was confirmed by polyacrylamide gel electrophoresis assay and square wave voltammetry ***,it is beneficial to immobilize single-stranded DNA(ssDNA)probes due to the large specific surface area of nanoporous gold,thereby improving the sensitivity of the *** Y-shaped ratio biosensor exhibited a wide detection range and can quantify the concentration of miR-18a in the range of 10 fmol/L–100 pmol/L,with a limit of detection of 0.211 fmol/L(S/N=3).Moreover,it exhibits excellent detection capabilities in serum samples since the biosensor showed a high selectivity toward the coexistence of miR-18a and ***,the prepared Y-shaped ratio biosensor is a highly sensitive and specific miR-18a detection tool,capable of identifying microscopic amounts of miR-18a in serum samples,providing great potential for early screening of NSCLC.

关键词： Nanoporous gold Y-shaped biosensor miR-18a NSCLC

SO_(2)-Insertion induced enantioselective oxysulfonylation to access β-chiral sulfones with quaternary carbon stereocenters

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Science China Chemistry 2024年第3期67卷 908-913页

作者： Jun Zhang xuefeng Wang Peiqi Wang Jiayuan Fang Shaoyu Li Jie Wu School of Pharmaceutical and Chemical Engineering&Institute for Advanced Studies Taizhou UniversityShanghai 200438China Department of Chemistry Fudan UniversityShanghai 200438China State Key Laboratory of Organometallic Chemistry Shanghai Institute of Organic ChemistryChinese Academy of SciencesShanghai 200032China School of Chemistry and Chemical Engineering Henan Normal UniversityXinxiang 453007China

β-Chiral sulfones are structural motifs widely found in natural products and bioactive molecules, while also serving as important intermediates for the synthesis of valuable chiral scaffolds. In contrast to the rapid growth of sulfur dioxide insertion chemistry over the last decade, enantioselective catalytic variants for accessing β-chiral sulfones, especially those bearing a quaternary carbon stereocenter, remain rare. Herein, we report an enantioselective copper/bisoxazoline catalyzed oxysulfonylation of alkenes to yield isoxazolinyl-containing β-chiral sulfones bearing quaternary carbon stereocenters with moderate to excellent yields and up to 96:4 er. The advantage and irreplaceability of this sulfur dioxide insertion catalytic system have been demonstrated by achieving transformations clearly unavailable with the previously reported sulfonyl chloride system. Additionally,the recoverability of the new-developed bisoxazoline ligand L9 and its reusability without any erosion of the catalytic activity and enantioselectivity further demonstrate the usefulness of the protocol.

关键词： β-chiral sulfones sulfur dioxide insertion oxysulfonylation copper catalysis enantioselectivity

Hesperidin ameliorates H_(2)O_(2)-induced bovine mammary epithelial cell oxidative stress via the Nrf2 signaling pathway

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Journal of Animal Science and biotechnology 2024年第4期15卷 1737-1750页

作者： Qi Huang Jiashuo Liu Can Peng xuefeng Han Zhiliang Tan CAS Key Laboratory for Agro‑Ecological Processes in Subtropical Region National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry ProductionHunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic ProcessInstitute of Subtropical AgricultureChinese Academy of SciencesChangsha 410125HunanChina State Key Laboratory of Animal Nutrition and Feeding Institute of Animal SciencesChinese Academy of Agricultural SciencesBeijing 100193China

background Hesperidin is a citrus flavonoid with anti-inflammatory and antioxidant potential. However, its protective effects on bovine mammary epithelial cells(b MECs) exposed to oxidative stress have not been *** In this study, we investigated the effects of hesperidin on H_(2)O_(2)-induced oxidative stress in b MECs and the underlying molecular mechanism. We found that hesperidin attenuated H_(2)O_(2)-induced cell damage by reducing reactive oxygen species(ROS) and malondialdehyde(MDA) levels, increasing catalase(CAT) activity, and improving cell proliferation and mitochondrial membrane potential. Moreover, hesperidin activated the Keap1/Nrf2/ARE signaling pathway by inducing the nuclear translocation of Nrf2 and the expression of its downstream genes NQO1 and HO-1, which are antioxidant enzymes involved in ROS scavenging and cellular redox balance. The protective effects of hesperidin were blocked by the Nrf2 inhibitor ML385, indicating that they were Nrf2 *** Our results suggest that hesperidin could protect b MECs from oxidative stress injury by activating the Nrf2 signaling pathway, suggesting that hesperidin as a natural antioxidant has positive potential as a feed additive or plant drug to promote the health benefits of bovine mammary.

关键词： bovine mammary epithelial cell Hesperidin Nrf2 signaling pathway Oxidative stress

Characteristics of long-distance mobile mRNAs from shoot to root in grafted plant species

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Horticultural Plant Journal 2024年第1期10卷 25-37页

作者： Mengmeng Fu Zhen Xu Huaying Ma Yifan Hao Ji Tian Yi Wang Xinzhong Zhang xuefeng Xu Zhenhai Han Ting Wu College of Horticulture China Agricultural UniversityBeijing 100193China Plant Science and Technology College Beijing University of AgricultureBeijing 102206China

Thousands of long-distance mobile mRNAs were identified from different grafting systems,based on high-throughput sequencing ***,the long-distance delivery of RNAs was proved to involve multiple ***,we analyzed the homology,motif,and tRNA-like structure(TLS)of long-distance mobile mRNAs identified by RNA-seq as well as the RNA-binding protein(RbP)in nine grafting combinations including Arabidopsis thaliana,Vitis vinifera,Cucumis sativus,Citrullus lanatus,Nicotiana benthamiana,Malus domestica,Pyrus spp.,Glycine max and Phaseolus *** several mRNAs were found to be shared in herbaceous,woody,and related species,the vast majority of long-distance mobile mRNAs were *** non-specific movement-related motifs were identified,while the TLS was not necessary for mRNA long distance *** addition,we found that RbPs were conserved among herbaceous and woody plants as well as related *** paper reports a further in-depth analysis of the endogenous mechanisms by which the species-specific transportable m RNAs were selected by bioinformatics,in order to provide insights for future research on long-distance mobile mRNAs.

关键词： Grafting combination Long-distance mobile mRNA TLS RNA binding proteins

An electrochemiluminescent magneto-immunosensor for ultrasensitive detection of hs-cTnI on a microfluidic chip

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Nanotechnology and Precision Engineering 2024年第3期7卷 13-23页

作者： Yun Hui Zhen Zhao Weiliang Shu Fengshan Shen Weijun Kong Shengyong Geng Zhen Xu Tianzhun Wu Wenhua Zhou xuefeng Yu Shenzhen Key Laboratory of Micro/Nano biosensing Shenzhen Institutes of Advanced TechnologyChinese Academy of SciencesShenzhen 518055China Shenzhen Institutes of Advanced Technology Chinese Academy of SciencesShenzhen 518055China biomedical Imaging Science and System Key Laboratory Chinese Academy of SciencesShenzhen 518055China

Sensitive detection and precise quantitation of trace-level crucial biomarkers in a complex sample matrix has become an important area of *** example,the detection of high-sensitivity cardiac troponin I (hs-cTnI) is strongly recommended in clinical guidelines for early diagnosis of acute myocardial *** on the use of an electrode modified by single-walled carbon nanotubes (SWCNTs) and a Ru(bpy)32+-doped silica nanoparticle (Ru@SiO2)/tripropylamine (TPA) system,a novel type of electrochemiluminescent (ECL) magnetoimmunosensor is developed for ultrasensitive detection of *** this approach,a large amount of[Ru(bpy)3]2+is loaded in SiO2(silica nanoparticles) as luminophores with high luminescent efficiency and SWCNTs as electrode surface modification material with excellent electrooxidation ability for ***,a hierarchical micropillar array of microstructures is fabricated with a magnet placed at each end to efficiently confine a single layer of immunomagnetic microbeads on the surface of the electrode and enable 7.5-fold signal enhancement In particular,the use of transparent SWCNTs to modify a transparent ITO electrode provides a two-order-of-magnitude ECL signal amplification.A good linear calibration curve is developed for hs-cTnI concentrations over a wide range from 10 fg/ml to 10 ng/ml,with the limit of detection calculated as 8.720 fg/ml (S/N=3).This ultrasensitive immunosensor exhibits superior detection performance with remarkable stability,reproducibility,and *** recoveries are obtained in the detection of hs-cTnI in human serum,providing a potentia analysis protocol for clinical applications.

关键词： Electrochemiluminescent magneto-immunosensor Microﬂuidic chip High-sensitivity cardiac troponin I Single-walled carbon nanotube [Ru(bpy)3]2+-doped silica nanoparticle

Semi-synthetic chondroitin sulfate CS-semi5 upregulates miR-122-5p,conferring a therapeutic effect on osteoarthritis via the p38/MMP13 pathway

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Acta Pharmaceutica Sinica b 2024年第8期14卷 3528-3542页

作者： Xiang Li Ya Zhou xuefeng Chen Hongjun Wang Shuang Yang Jun Yang Yunfeng Song Zhehui Zhao Haijing Zhang Lianqiu Wu State Key Laboratory of Digestive Health Institute of Materia MedicaChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100050China Tide Pharmaceutical Co. Ltd.Beijing 100176China Camford Royal School Beijing 100093China

Osteoarthritis(OA)is an aging-associated disease characterized by joint stiffness pain and destroyed articular *** treatments for OA are limited to alleviating various OA *** is a lack of drugs available in clinical practice that can truly repair cartilage ***,we developed the chondroitin sulfate analog CS-semi5,semi-synthesized from chondroitin sulfate *** vivo,CS-semi5 alleviated inflammation,provided analgesic effects,and protected cartilage in the modified Hulth OA rat model and papain-induced OA rat model.A bioinformatics analysis was performed on samples from OA patients and an exosome analysis on papain-induced OA rats,revealing miR-122-5p as the key regulator associated with CS-semi5 in OA *** prediction revealed that miR-122-5p acted on the 30-untranslated region of p38 mitogen-activated protein kinase,which was related to MMP13 *** in vitro experiments revealed that CS-semi5 effectively reduced cartilage degeneration and maintained matrix homeostasis by inhibiting matrix breakdown through the miR-122-5p/p38/MMP13 axis,which was further validated in the articular cartilage of OA *** is the first study to investigate the semi-synthesized chondroitin sulfate CS-semi5,revealing its cartilageprotecting,anti-inflammatory,and analgesic properties that show promising therapeutic effects in OA via the miR-122-5p/p38/MMP13 pathway.

关键词： Chondroitin sulfate Cartilage Osteoarthritis Extracellular matrix Inflammation miRNA p38 MMP13

Identification of key genes and metabolites involved in meat quality performance in Qinchuan cattle by WGCNA

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Journal of Integrative Agriculture 2024年第11期23卷 3923-3937页

作者： Hengwei Yu Zhimei Yang Jianfang Wang Huaxuan Li xuefeng Li Entang Liang Chugang Mei Linsen Zan College of Animal Science and Technology Northwest A&F University College of Grassland Agriculture Northwest A&F University National beef Cattle Improvement Center

Understanding the genetic and metabolic elements that impact meat quality is crucial to improving production and meeting consumer demands in the beef *** in meat quality among various muscle areas in beef cattle can impact pricing in the *** progress in genomics,the specific genes and metabolites that affect meat quality characteristics in Qinchuan cattle remain inadequately ***,this study aims to evaluate the meat quality characteristics of four specific muscle locations (tenderloin,striploin,high rib,and ribeye muscles) in Qinchuan bulls,including 10 traits (total protein content (TPC),intramuscular fat (IMF),non-esterified fatty acid (NEFA),meat color (L*,a*,and b*),shear force (SF),cooking loss (CL),pH0,and pH24).This experiment uses transcriptome,metabolome sequencing,and sophisticated analytical methodologies such as weighted gene co-expression network analysis (WGCNA) and protein–protein interaction networks (PPI) to identify the key genes and metabolites associated with specific *** findings highlight three notable genes (NDUFAb1,NDUFA12,and NDUFb7) linked to intramuscular fat (IMF),three key genes (CSRP3,ACAA3,and ACADVL) correlated with non-esterified fatty acids (NEFA),and one crucial gene (CREbbP) influencing meat *** conclusion,this investigation offers a new perspective on the differences in bovine muscle locations and contributes to the molecular understanding of bovine meat *** research endeavors could delve deeper into the identified genes and pathways to enhance beef cattle’s quality and yield.

关键词： meat quality performance WGCNA cattle transcriptome metabolome