An electrochemiluminescent magneto-immunosensor for ultrasensitive detection of hs-cTnI on a microfluidic chip
作者机构：Chinese Acad Sci Shenzhen Inst Adv Technol Shenzhen Key Lab Micro Nano Biosensing Shenzhen 518055 Peoples R China Chinese Acad Sci Biomed Imaging Sci & Syst Key Lab Shenzhen 518055 Peoples R China Chinese Acad Sci Shenzhen Inst Adv Technol Shenzhen 518055 Peoples R China
出 版 物：《NANOTECHNOLOGY AND PRECISION ENGINEERING》 (纳米技术与精密工程（英文）)
年 卷 期：2024年第7卷第3期
页 面：13-23页
核心收录：
学科分类：08[工学] 0805[工学-材料科学与工程（可授工学、理学学位）] 080502[工学-材料学] 0702[理学-物理学]
基 金：National Natural Science Foundation of China [62001460, 31971368, 12202461, 22104148] Guangdong Regional Joint Funds for Young Scientists [2020A1515110201, 2020A1515110368] Guangdong Provincial General Funding [2021A1515220156] Guangdong Basic and Applied Basic Research Funding-Regional Joint Fund [2020B1515120040] Shenzhen Science and Technology Research Funding [JSGG20201103153801005, JSGG20191115141601721, ZDSYS20220527171406014, JCYJ20220818101412027, JCYJ20200109115635440, JCYJ20200109115408041]
主 题：Electrochemiluminescent magneto-immunosensor Microfluidic chip High-sensitivity cardiac troponin I Single-walled carbon nanotube [Ru(bpy)(3)](2+)-doped silica nanoparticle
摘 要：Sensitive detection and precise quantitation of trace-level crucial biomarkers in a complex sample matrix has become an important area of research. For example, the detection of high-sensitivity cardiac troponin I (hs-cTnI) is strongly recommended in clinical guidelines for early diagnosis of acute myocardial infarction. Based on the use of an electrode modified by single-walled carbon nanotubes (SWCNTs) and a Ru(bpy)(3)(2+)-doped silica nanoparticle (Ru@SiO2)/tripropylamine (TPA) system, a novel type of electrochemiluminescent (ECL) magneto-immunosensor is developed for ultrasensitive detection of hs-cTnI. In this approach, a large amount of [Ru(bpy)(3)](2+) is loaded in SiO2 (silica nanoparticles) as luminophores with high luminescent efficiency and SWCNTs as electrode surface modification material with excellent electrooxidation ability for TPA. Subsequently, a hierarchical micropillar array of microstructures is fabricated with a magnet placed at each end to efficiently confine a single layer of immunomagnetic microbeads on the surface of the electrode and enable 7.5-fold signal enhancement. In particular, the use of transparent SWCNTs to modify a transparent ITO electrode provides a two-order-of-magnitude ECL signal amplification. A good linear calibration curve is developed for hs-cTnI concentrations over a wide range from 10 fg/ml to 10 ng/ml, with the limit of detection calculated as 8.720 fg/ml (S/N = 3). This ultrasensitive immunosensor exhibits superior detection performance with remarkable stability, reproducibility, and selectivity. Satisfactory recoveries are obtained in the detection of hs-cTnI in human serum, providing a potential analysis protocol for clinical applications. (c) 2024 Author(s). All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://***/licenses/by/4.0/).
维普期刊数据库