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Glycogen Synthase Kinase 3β Inhibitor (2'Z,3'E)-6-Bromo-indirubin-3'-Oxime Enhances Drug Resistance to 5-Fluorouracil Chemotherapy in Colon Cancer Cells

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Chinese Journal of Cancer Research 2012年第2期24卷 116-123页

作者： Kun-ping Liu Feng Luo Si-ming Xie Li-juan Tang Mei-xiang Chen xue-fang Wu xue-yun zhong Tong Zhao Department of Pathology Nanfang HospitalSouthern Medical UniversityGuangzhou 510515China Department of Pathology Qingyuan HospitalMedical CollegeJinan UniversityQingyuan 511518China Department of Pathology Medical CollegeJinan UniversityGuangzhou 510632China Department of Pathology School of Basic Medical ScienceSouthern Medical UniversityGuangzhou 510515China

Objective： To explore the effects and mechanism of glycogen synthase kinase 3β （GSK-313） inhibitor （2＇Z,3＇E）-6-bromo-indirubin-3＇-oxime （BIO） on drug resistance in colon cancer cells. Methods： The colon cancer SW480 and SW620 cells were treated with BIO, 5-fluorouracil （5-FU） and BIO/5-FU, separately. Cell cycle distribution, apoptosis level and efflux ability of rhodamine 123 （Rh123） were detected by flow cytometry. The protein expressions of P-glycoprotein （P-gp）, multidrug resistance protein 2 （MRP2）, thymidylate synthase （TS）, β-catenin, E2F-1 and βcl-2 were detected by Western blot. β-catenin and P-gp were stained with double immunofluorescence and observed under a confocal microscope. Results： BIO up-regulated β-catenin, P-gp, MRP2 and TS, enhanced the efflux ability of Rh123, decreased Bcl-2 protein and gave the opposite effect to E2F-1 protein in SW480 and SW620 ceils. Furthermore, BIO significantly inhibited cell apoptosis, increased S and G2/M phase cells, and reduced the cell apoptosis induced by 5-FU in SW480 cells, whereas the effects were slight or not obvious in SW620 cells. Conclusion： GSK-3β was involved in drug resistance regulation, and activation of β-catenin and inhibition of E2F-1 may be the most responsible for the enhancement of 5-FU chemotherapy resistance induced by GSK-β inhibitor β10 in colon cancer.

关键词： Colorectal neoplasms Drug resistance Glycogen synthase kinase 313 Fluorouracil 13-catenin E2F-1

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Role of DJ-1-induced PTEN down-regulation in migration and invasion of human glioma cells

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Chinese Journal of Cancer 2010年第12期29卷 988-994页

作者： Mao Fang xue-yun zhong Bin Du Chen-Li Lin Feng Luo Li-Juan Tang Juan Chen Department of Pathology Medical College Jinan University Guangzhou Guangdong 510632 P. IR. China

Background and Objective: DJ-1, a suppressor of PTEN, promotes metastasis of different tumors, but its function and mechanisms in glioma metastasis remain unclear. This study aimed to investigate the effect of the DJ-1 protein on the migration and invasion of human glioma cells, and to explore potential mechanisms. Methods: The eukaryotic expression vector pEGFP/DJ-1 and small interfering RNA (siRNA) were constructed and transfected into human glioma SWO-38 cells. The expression of DJ-1 and PTEN in SWO-38 cells were detected by Western blot. Cell migration and invasion were detected by transwell assay. Results: After transfection of pEGFP/DJ-1, the expression of DJ-1 (1.28 ± 0.15 vs. 0.89 ± 0.04, P < 0.05) and focal adhesion kinase (FAK) phosphorylation (0.76 ± 0.12 vs. 0.51 ± 0.04, P < 0.05) were increased, whereas the expression of PTEN (0.74 ± 0.2 vs. 1.04 ± 0.14, P < 0.05) was suppressed. After transfection of DJ-1 siRNA, both DJ-1 (0.33 ± 0.04 vs. 0.88 ± 0.06, P < 0.05) and p-FAK levels (0.33 ± 0.01 vs. 0.44 ± 0.05, P < 0.05) were decreased, but PTEN expression (1.1 ± 0.06 vs. 0.81 ± 0.12, P < 0.05) was increased. Transwell assay data showed that pEGFP/DJ-1 transfection promoted SWO-38 cell migration (57.2 ± 6.50 vs. 40.4 ± 5.0, P < 0.05) and invasion (54.6 ± 4.9 vs. 27 ± 6.7, P < 0.05), whereas DJ-1 siRNA transfection inhibited SWO-38 cells migration (54.4 ± 6.9 vs. 73.4 ± 7.6, P < 0.05) and invasion (44.6 ± 5.8 vs. 69.2 ± 9.2, P < 0.05). Conclusion: Over-expression of DJ-1 promotes SWO-38 cell migration and invasion possibly through the DJ-1 and the PTEN/FAK pathway.

关键词： PTEN基因胶质瘤细胞细胞迁移侵袭人脑社会工作细胞转染入侵检测

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Management of intra-articular fracture of the fingers via mini external fixator combined with limited internal fixation

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Chinese Medical Journal 2009年第21期122卷 2616-2619页

作者： LI Wen-jun TIAN Wen TIAN Guang-lei CHEN Shan-lin ZHANG Chang-qing xue yun-hao LI zhong-zhe ZHU Yin Department of Hand Surgery Beijing Jishuitan Hospital Beijing 100035 China

Background Intra-articular fractures of the fingers are common problems to emergency physicians and hand surgeons Inappropriate management of these injuries may result in chronic pain, stiffness, deformity, or post traumatic arthritis. Ideal treatment necessitates the restoration of a stable and congruent joint that will allow early mobilization. The purpose of this study was to investigate the results of intra-articular fracture of the fingers by mini external fixator combined with limited internal fixation. Methods From May 2005 to May 2007, a total of 26 patients with intra-articular fracture of the fingers were treated by mini external fixator combined with limited internal fixation. Of the 26 cases, 11 involved in metacarpophalangeal joint, and 15 interphalangeal joint in proximal interphalangeal. Kirschner wire, mini wire and absorbable suture were used for limited internal fixation. All patients were followed up and patients were accomplished with total active motion （TAM） of fingers. Results All patients were reviewed by an independent observer. The mean follow up was 13 months （range 9 to 24 months）. Subjective, objective and radiographic results were evaluated. X-ray films revealed fracture union and the average radiographic union time was 7 weeks with a range of 5-12 weeks and the phalange shortening or rotation in 2 cases, joint incongruity （less than 1 mm） and joint space narrowing in 3 cases respectively. Phalangeal shortening or rotation was observed in 2 cases and joint incongruity or joint space narrowing was observed in 3 cases. An artificial implant was performed on one case for traumatic arthritis 1.5 years after surgery. Based on TAM the overall good-excellent rate of joint motion function was 80.8%. Conclusion Mini external fixator combined with limited internal fixation is a reliable and effective method for treatment of intra-articular fracture of the fingers.

关键词： external fixator intra-articular fracture internal fixation finger

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电磁轨道发射状态下的复合导轨动态响应研究

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兵工学报 2017年第4期38卷 651-657页

作者：田振国孟晓永安雪云白象忠燕山大学建筑工程与力学学院河北省重型装备与大型结构力学可靠性重点实验室河北秦皇岛066004

电磁炮导轨中通入电流后,电枢受到电磁力作用沿轨道移动,由于导轨受到安培力和电枢压力的作用,会引起轨道内表面的磨损、刨削现象,限制了电磁轨道炮的使用寿命。而铜基复合轨道可以同时具备有良好的导电性能、耐烧蚀能力以及较高的强度... 详细信息

电磁炮导轨中通入电流后,电枢受到电磁力作用沿轨道移动,由于导轨受到安培力和电枢压力的作用,会引起轨道内表面的磨损、刨削现象,限制了电磁轨道炮的使用寿命。而铜基复合轨道可以同时具备有良好的导电性能、耐烧蚀能力以及较高的强度,在大功率多次发射的电磁轨道炮中具有较好的应用前景。将复合导轨简化为弹性地基梁,并对其受力特点进行力学分析,应用二维Fourier积分变换,得到复合导轨在移动荷载作用下动态挠度的一般解,进而得到电磁轨道发射状态下复合导轨的动态响应。分析了复合轨道的几何尺寸、通入电压等参量对复合导轨动态响应的影响。计算结果表明:电枢压力对轨道变形的影响远大于轨道间斥力的影响;合理的复合层厚度占比可以改善轨道的动态性能。

关键词：兵器科学与技术复合轨道动态响应 Fourier变换移动荷载弹性地基梁

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应用组织芯片检测食管鳞状细胞癌中APC、β-catenin、E-cadherin与cyclin D1的表达及其意义

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癌症 2009年第1期28卷 49-53页

作者：彭辉钟雪云刘坤平李素梅暨南大学医学院病理教研室广东广州510632 暨南大学医学院第五附属医院病理科广东清远511500

背景与目的:食管鳞状细胞癌(esophageal squamous cell carcinoma,ESCC)的发生是个多因素多阶段的演进过程。Wnt信号转导通路在肿瘤发生发展中起重要作用。本研究探讨4种Wnt通路上的蛋白在食管鳞癌发生中的作用及在早期诊断中的意义。方... 详细信息

背景与目的:食管鳞状细胞癌(esophageal squamous cell carcinoma,ESCC)的发生是个多因素多阶段的演进过程。Wnt信号转导通路在肿瘤发生发展中起重要作用。本研究探讨4种Wnt通路上的蛋白在食管鳞癌发生中的作用及在早期诊断中的意义。方法:制作由199例食管鳞癌组织与其癌旁164例正常粘膜、34例基底细胞增生和30例不典型增生上皮组成的组织芯片,应用免疫组化方法检测APC、β-catenin、E-cadherin、cyclin D1的表达情况。结果:APC和E-cadherin在ESCC阳性率分别是69.6%、19.6%,均低于各自正常组(98.0%、96.3%,均为P0.05),与E-cadherin呈负相关(r=-0.31,P<0.01),与cyclin D1呈正相关(r=0.49,P<0.01)。结论:APC、E-cadherin、β-catenin和cyclin D1可能在ESCC发生过程中起重要作用。β-catenin、E-cadherin和cyclin D1的表达检测有助于ESCC的早期诊断。

关键词：食管肿瘤组织芯片 Wnt通路信号转导 APC β-catenin E-cadherin cyclin D1 诊断

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肿瘤耐药相关蛋白及β-catenin在食管鳞状细胞癌中的表达及意义

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癌症 2010年第3期29卷 323-329页

作者：甘思远钟雪云谢思明李素梅彭辉罗枫暨南大学医学院病理学教研室广东广州510632 暨南大学医学院人体解剖学系广东广州510632 广东省中医院病理科广东州510375

背景与目的:随着化疗药物在肿瘤临床治疗中的广泛应用,肿瘤对化疗药物产生多药耐药性的问题越来越突出,已成为肿瘤联合化疗失败的主要原因之一。本研究检测β-catenin以及肿瘤耐药相关蛋白MRP2、P-gp、Bcl-2在食管鳞状细胞癌(esophageal... 详细信息

背景与目的:随着化疗药物在肿瘤临床治疗中的广泛应用,肿瘤对化疗药物产生多药耐药性的问题越来越突出,已成为肿瘤联合化疗失败的主要原因之一。本研究检测β-catenin以及肿瘤耐药相关蛋白MRP2、P-gp、Bcl-2在食管鳞状细胞癌(esophageal squamous cellcar cinoma,ESCC)中的表达,探讨它们在ESCC的多药耐药发生发展中的作用和相互关系。方法:运用组织芯片技术、免疫组织化学方法以及图像分析技术检测582例ESCC及其癌旁294例正常黏膜、92例单纯细胞增生和87例不典型增生上皮组成的组织芯片中MRP2、P-gp、β-catenin及Bcl-2的表达情况,并分析其与各临床病理参数之间的关系。结果:MRP2、Bcl-2在ESCC中的累积光密度(integral optical density,IOD)值[分别为(195.7±175.9)×103和(90.5±112.5)×103]显著高于其在癌旁正常组织中的IOD值[分别为(104.8±86.1)×103和(25.2±46.6)×103],差异有统计学意义(P<0.01);P-gp、β-catenin在ESCC中的IOD值[分别为(57.7±75.5)×103和(32.0±47.0)×103]显著低于其在癌旁正常组织中的IOD值[分别为(114.8±106.6)×103和(46.1±35.7)×103],差异有统计学意义(P<0.01);随着ESCC的分化程度按高分化-中分化-低分化顺序依次改变,MRP2的IOD值依次递增,β-catenin在低分化ESCC中的IOD值大于其在中、高分化ESCC中的IOD值,Bcl-2在高分化ESCC中的IOD值低于其在中、低分化ESCC中的IOD值,差异有统计学意义(P<0.01);β-catenin、Bcl-2在ESCC浸润至黏膜层的病例的癌组织标本中的IOD值大于其在ESCC浸润至肌层或浆膜层的病例的癌组织标本中的IOD值,差异有统计学意义(P<0.01);有淋巴结转移的Bcl-2的IOD值显著高于无淋巴结转移(P<0.01);ESCC中P-gp的表达分别与MRP2、Bcl-2的表达呈明显正相关(r=0.288和r=0.253,P<0.01)。结论:P-gp与MRP2及Bcl-2可作为ESCC多药耐药的预测因子。β-catenin可能在ESCC的发生发展中起着重要作用。

关键词：食管肿瘤鳞状细胞癌 MRP2 P-gp β-catenin Bcl-2 组织芯片免疫组织化学图像分析技术

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Effect of land-use and land-cover change on nutrients in soil in Bashang area,China

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Journal of Environmental Sciences 2003年第4期15卷 548-553页

作者： SHENG xue-bin SUN Jian-zhong LIU yun-xia Research Center for Eco Environmental Sciences Chinese Academy of SciencesBeijing 100085China.

The Bashang area is a water source area and ecological barrier zone for Beijing and Tianjin. The area is located at 200 km from Beijing and Tianjin and is a typical agriculture pasture interlacing zone, in which the eco environment is vulnerable and hence it is sensitive to environmental change. The area is relatively lagged in social and economic development, where the traditional cultivation mode is predominated in agriculture and animal husbandry, but the disturbance by human activity is relatively small. Therefore, in order to reveal the interrelation between eco environmental change in the area and environmental change in Beijing and Tianjin area, it is fairly necessary to study the effect of land use and land cover change on nutrients in soil in this area. According to the actual situation of changed land use for the limited time period, five series of changed land plots were selected and 4 samples were collected from each series for the study. The samples were collected from different soil forming levels at the same site in different time. Analysis of the collected samples indicates that in the process of change of land use and land cover, the nutrients in soil, such as organic matter, total N, total P, total K, and available N, P, K, and B, Mo, Mn, Zn, Cu, and Fe, have regularly changed. When the land had changed from grassland and non irrigated farmland into woodland, and from non irrigated farmland into irrigated field, the nutrients in soil totally increased. But there exists some exception, i.e. quick acting N, P, K, and some microelements have appeared to be inconsistent with the mentioned above regularity in some cases.

关键词： Bashang area land use change variation of nutrients

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不同加载速度下巴厘菠萝果皮穿刺力学特性研究

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农机化研究 2025年第3期47卷 169-175页

作者：薛忠潘睿张秀梅中国热带农业科学院南亚热带作物研究所广东湛江524091 中国热带农业科学院农业机械研究所广东湛江524091 农业农村部热带果树生物学重点实验室广东湛江524091

对菠萝果皮穿刺力学特性进行研究,旨在为果实品质评价提供参考,为菠萝采收、加工设备优化改进提供参考,为减轻菠萝果损伤提供理论依据。以巴厘菠萝果皮为研究对象,根据菠萝生长规律将果皮划分为上、中、下3个部位,基于WSW-50E微机控制... 详细信息

对菠萝果皮穿刺力学特性进行研究,旨在为果实品质评价提供参考,为菠萝采收、加工设备优化改进提供参考,为减轻菠萝果损伤提供理论依据。以巴厘菠萝果皮为研究对象,根据菠萝生长规律将果皮划分为上、中、下3个部位,基于WSW-50E微机控制电子万能试验机,选取2、5、10、20、50 mm/min等5个加载速度对菠萝不同部位进行穿刺力学特性试验,分析不同加载速度、不同穿刺部位和不同穿刺压头截面对果皮穿刺强度的影响。试验结果表明:果皮中部穿刺强度最低,下部穿刺强度最高;压头截面与不同穿刺部位对果皮穿刺强度均呈现负相关极显著(p<0.01),不同穿刺部位对果皮穿刺强度均呈现正相关极显著(p<0.01),压头截面与穿刺部位对穿刺强度影响极显著(p<0.01)。由此得出结论:果皮穿刺强度与压头截面、穿刺部位呈极显著相关关系,加载速度与穿刺强度呈不相关关系。

关键词：菠萝果皮加载速度压头截面穿刺强度

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Numerical simulation of slurry jets using mixture model

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Water Science and Engineering 2013年第1期6卷 78-90页

作者： Wen-xin HUAI Wan-yun xue zhong-dong QIAN State Key Laboratory of Water Resources and Hydropower Engineering Science Wuhan University

Slurry jets in a static uniform environment were simulated with a two-phase mixture model in which flow-particle interactions were considered. A standard k-e turbulence model was chosen to close the governing equations. The computational results were in agreement with previous laboratory measurements. The characteristics of the two-phase flow field and the influences of hydraulic and geometric parameters on the distribution of the slurry jets were analyzed on the basis of the computational results. The calculated results reveal that if the initial velocity of the slurry jet is high, the jet spreads less in the radial direction. When the slurry jet is less influenced by the ambient fluid （when the Stokes number St is relatively large）, the turbulent kinetic energy k and turbulent dissipation rate e, which are relatively concentrated around the jet axis, decrease more rapidly after the slurry jet passes through the nozzle. For different values of St, the radial distributions of streamwise velocity and particle volume fraction are both self-similar and fit a Gaussian profile after the slurry jet fully develops. The decay rate of the particle velocity is lower than that of water velocity along the jet axis, and the axial distributions of the centerline particle streamwise velocity are self-similar along the jet axis. The pattern of particle dispersion depends on the Stokes number St. When St = 0.39, the panicle dispersion along the radial direction is considerable, and the relative velocity is very low due to the low dynamic response time. When St = 3.08, the dispersion of particles along the radial direction is very little, and most of the particles have high relative velocities along the streamwise direction.

关键词： slurry jet," numerical simulation," two-phase mixture model Stokes number,"flow-particle interaction

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On exponential stability for systems with state delays

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2007年第8期8卷 1296-1303页

作者： CHEN yun xue An-ke GE Ming WANG Jian-zhong LU Ren-quan Institute of Operational Research and Cybernetics Hangzhou Dianzi University Hangzhou 310018 China National Laboratory of Industrial Control Technology. Zhejiang University Hangzhou 310027 China Institute of Information and Control Hangzhou Dianzi University. Hangzhou 310018 China

This paper considers the issue of delay-dependent exponential stability for time-delay systems. Both nominal and uncertain systems are investigated. New sufficient conditions in terms of linear matrix inequalities(LMIs) are obtained. These criteria are simple owing to the use of an integral inequality. The model transformation approaches,bounding techniques for cross terms and slack matrices are all avoided in the derivation. Rigorous proof and numerical examples showed that the proposed criteria and those based on introducing slack matrices are equivalent.

关键词： Exponential stability Uncertain time-delay systems Integral inequality Slack matrix Linear matrix inequality (LMI)

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