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计算机与应用化学 1999年第5期16卷 353-354页

作者： FANG xue Liang\ zhang wei\ WEN Hao\ XU Zhi\|Hong (Laboratory of Computer Chemistry, Institute of Chemical Metallurgy, Chinese Academy of Sciences, Beijing\ 100080) 中科院化工冶金所北京

A methodology study is performed in order to get access to databases and calculations via Internet. The data retrieved from a database or calculated from a program can be converted into HTML documents using the HTML extension files (* htx) as the template and sent back to the web browsers. As an example, a database and a program package for thermodynamic and equilibrium properties data retrieval and calculations in engineering chemistry are developed on the web server.

关键词： Database Equilibrium calculation Internet

Müller cells are activated in response to retinal outer nuclear layer degeneration in rats subjected to simulated weightlessness conditions

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Neural Regeneration Research 2025年第7期20卷 2116-2128页

作者： Yuxue Mu Ning zhang Dongyu wei Guoqing Yang Lilingxuan Yao Xinyue Xu Yang Li Junhui xue Zuoming zhang Tao Chen Aerospace Clinical Medical Center School of Aerospace MedicineAir Force Medical UniversityXi’anShaanxi ProvinceChina Department of Aviation Medicine Xijing HospitalAir Force Medical UniversityXi’anShaanxi ProvinceChina Department of Emergency Medicine Wuhan No.1 HospitalWuhanHubei ProvinceChina Third Regiment School of Basic MedicineAir Force Medical UniversityXi’anShaanxi ProvinceChina Fourth Regiment School of Basic MedicineAir Force Medical UniversityXi’anShaanxi ProvinceChina

A microgravity environment has been shown to cause ocular damage and affect visual acuity,but the underlying mechanisms remain ***,we established an animal model of weightlessness via tail suspension to examine the pathological changes and molecular mechanisms of retinal damage under *** 4 weeks of tail suspension,there were no notable alterations in retinal function and morphology,while after 8 weeks of tail suspension,significant reductions in retinal function were observed,and the outer nuclear layer was thinner,with abundant apoptotic *** investigate the mechanism underlying the degenerative changes that occurred in the outer nuclear layer of the retina,proteomics was used to analyze differentially expressed proteins in rat retinas after 8 weeks of tail *** results showed that the expression levels of fibroblast growth factor 2(also known as basic fibroblast growth factor)and glial fibrillary acidic protein,which are closely related to Müller cell activation,were significantly *** addition,Müller cell regeneration and Müller cell gliosis were observed after 4 and 8 weeks,respectively,of simulated *** findings indicate that Müller cells play an important regulatory role in retinal outer nuclear layer degeneration during weightlessness.

关键词： glial fibrous acidic protein gliosis Müller cells nerve growth factor neural differentiation neurodegeneration proteomic retinal degeneration retinal outer nuclear layer simulated weightlessness

Elevation of tumor necrosis factor-α,interleukin-1β and interleukin-6 levels in aortic intima of Chinese Guizhou minipigs with streptozotocin-induced diabetes

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Chinese Medical Journal 2007年第6期120卷 479-484页

作者： LU Lin zhang Qi PU Li-jin XU xue-wei zhang Rui-yan zhang Jian-sheng HU Jian YANG Zheng-kun LU An-kang DING Feng-hua SHEN Jie CHEN Qiu-jin LOU Sheng FANG Dan-hong SHEN wei-feng Department of Cardiology Ruijin Hospital Institute of Cardiovascular Disease Medical School of Jiaotong University Shanghai 200025 China

Background Large animal models with toxin-mediated pancreatic damage have been used extensively in researches with respect to diabetes mellitus and cardiovascular diabetic complications. The present study aimed to establish Chinese Guizhou minipig models with streptozotocin （STZ）-induced diabetes and characterize the animal models by analyzing inflammatory cytokine levels in aortic wall, such as tumor necrosis factor （TNF）-α, interleukin-1β （IL-1β） and interleukin-6 （IL-6）. Methods Twenty-two male Chinese Guizhou minipigs （age, 4 to 6 months; weight, 20 kg to 30 kg） were divided into STZ-induced diabetic group （n=-12） and control group （n=-10）. STZ （125 mg/kg） was administrated to induce hyperglycemia and afterwards insulin was used to control fasting blood glucose levels below 10 mmol/L. Oral glucose tolerance test （OGTT） was performed before and one month after STZ administration and serum concentrations of alanine transaminase, asparegine transaminase, albumin, blood urea nitrogen, creatinine, lipids and white blood cell count were measured before and six months later. Animals in both groups were euthanized after six months and pancreas was examined immunohistochemically for islet 13 cells. Aortic intima of diabetic minipigs and controls was analyzed for TNF-α level in tissue conditioned medium by Western blot. TNF-α, IL-β and IL-6 mFINA levels in aortic intima were assayed by reverse transcription and polymerase chain reaction （FIT-PCR）. Results Significant elevation in serum glucose levels was observed one month and six months after STZ induction （P〈0.001） and markedly increased OGTT values were noted, compared with baseline data. The normal pancreas had many irregular sized islets and small clusters of islet β cells, while in pancreas of diabetic minipigs islet β ceils almost disappeared. No statistical difference was notified in serum concentrations of biochemical examinations before and six months after STZ induction. Westem blot demonstrated dramatically in

关键词： diabetes meUitus streptozotocin tumor necrosis factor-α interleukin-1β interleukin-6

Argo温度数据的ConvGRU模型预测分析

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海洋环境科学 2022年第4期41卷 628-635页

作者：张雪薇韩震上海海洋大学海洋科学学院上海201306 上海河口海洋测绘工程技术研究中心上海201306

Argo(Array for Real-time Geostrophic Oceanography)是海洋环境信息的重要来源之一,可通过自动剖面浮标、卫星定位和数据同化等技术获取大范围全球海洋上层之间的海温剖面资料。本文利用ConvGRU(Convolutional Gate Recurrent Unit)作... 详细信息

Argo(Array for Real-time Geostrophic Oceanography)是海洋环境信息的重要来源之一,可通过自动剖面浮标、卫星定位和数据同化等技术获取大范围全球海洋上层之间的海温剖面资料。本文利用ConvGRU(Convolutional Gate Recurrent Unit)作为Argo温度的预测模型,以西北太平洋部分海域为研究区域,选取2004-2018年Argo数据作为训练数据,对2019年的0 m、50 m、100 m、200 m和300 m不同深度位置水平剖面进行了预测分析。研究结果表明:ConvGRU模型对Argo温度数据的变化趋势具有较好的模拟能力;预测模型的训练集和验证集的RMSE(root mean squared error)均值分别为0.0462℃和0.0463℃,MAE(mean absolute error)均值分别为0.0442℃和0.0450℃,其Acc(accuracy)都在99%以上;对于预测评估,RE(relative error)的误差范围为0.0228~0.0427,预测变化的空间特性与真实值的吻合程度较高。

关键词： Argo数据 ConvGRU 温度预测模型

The interaction between KIF21A and KANK1 regulates dendritic morphology and synapse plasticity in neurons

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Neural Regeneration Research 2025年第1期20卷 209-223页

作者： Shi-Yan Sun Lingyun Nie Jing zhang xue Fang Hongmei Luo Chuanhai Fu Zhiyi wei Ai-Hui Tang Hefei National Research Center for Physical Sciences at the Microscale CAS Key Laboratory of Brain Function and DiseaseMinistry of Education Key Laboratory for Membrane-less Organelles and Cellular DynamicsDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefeiAnhui ProvinceChina Institute of Artificial Intelligence Hefei Comprehensive National Science CenterHefeiAnhui ProvinceChina CAS Center for Excellence in Molecular Cell Sciences Division of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefeiAnhui ProvinceChina Department of Neurobiology School of Life SciencesSouthern University of Science and TechnologyShenzhenGuangdong ProvinceChina Brain Research Center Southern University of Science and TechnologyShenzhenGuangdong ProvinceChina

Morphological alterations in dendritic spines have been linked to changes in functional communication between neurons that affect learning and ***-4 KIF21A helps organize the microtubule-actin network at the cell cortex by interacting with KANK1;however,whether KIF21A modulates dendritic structure and function in neurons remains *** this study,we found that KIF21A was distributed in a subset of dendritic spines,and that these KIF21A-positive spines were larger and more structurally plastic than KIF21A-negative ***,the interaction between KIF21A and KANK1 was found to be critical for dendritic spine morphogenesis and synaptic *** of either KIF21A or KANK1 inhibited dendritic spine morphogenesis and dendritic branching,and these deficits were fully rescued by coexpressing full-length KIF21A or KANK1,but not by proteins with mutations disrupting direct binding between KIF21A and KANK1 or binding between KANK1 and *** down KIF21A in the hippocampus of rats inhibited the amplitudes of long-term potentiation induced by high-frequency stimulation and negatively impacted the animals’cognitive *** together,our findings demonstrate the function of KIF21A in modulating spine morphology and provide insight into its role in synaptic function.

关键词： actin cytoskeleton dendrite KANK1 KIF21A microtubule spine morphology spine synaptic plasticity talin1

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小麦原生质体高效转化体系的建立

植物遗传资源学报 2017年第1期18卷 117-124页

作者：刘鑫魏学宁张学文张增艳湖南农业大学生物科学技术学院长沙410128 中国农业科学院作物科学研究所/农作物基因资源与基因改良国家重大科学工程/农业部麦类生物学与遗传育种重点实验室北京100081

植物原生质体广泛应用于植物基因功能研究中,包括瞬时基因表达、亚细胞定位、蛋白互作和蛋白活性分析等。当前,小麦基因的亚细胞定位和功能分析,大多利用模式植物拟南芥等异源的原生质体,易于造成研究结果的不准确。为避免这种情况,小... 详细信息

植物原生质体广泛应用于植物基因功能研究中,包括瞬时基因表达、亚细胞定位、蛋白互作和蛋白活性分析等。当前,小麦基因的亚细胞定位和功能分析,大多利用模式植物拟南芥等异源的原生质体,易于造成研究结果的不准确。为避免这种情况,小麦原生质体制备及高效转化体系的建立与应用是必需的。在PEG介导的小麦原生质体转化过程中,原生质体分泌的核酸酶大量降解质粒DNA,转化效率的提高因此受到阻碍。为了建立小麦原生质体的高效转化体系,本文测试了抑制胞外核酸酶活性的因素和提高质粒DNA浓度等多个条件对转化效率的影响。结果表明,转化过程中加入双倍用量的质粒DNA进行转化,且始终保持低温环境(1℃)用以抑制核酸酶酶活性,可以使小麦原生质体的转化效率提高至85%。本文还将该系统成功地应用于2个小麦抗病相关蛋白的亚细胞定位研究,证明了该系统的高效性和实用性。该研究对未来相关研究有一定参考价值。

关键词：小麦原生质体核酸酶活性原生质体转化效率抗病相关基因亚细胞定位

Influence of introducing Zr,Ti,Nb and Ce elements on externally solidified crystals and mechanical properties of high-pressure die-casting Al–Si alloy

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International Journal of Minerals,Metallurgy and Materials 2025年第1期32卷 147-153页

作者： Junjie Li Wenbo Yu Zhenyu Sun weichen Zheng Liangwei zhang Yanling xue Wenning Liu Shoumei Xiong Center of Materials Science and Engineering School of Mechanical and Electronic Control EngineeringBeijing Jiaotong UniversityBeijing 100044China Traffic&Transportation Engineering Central South UniversityChangsha 410075China Shanghai Synchrotron Radiation Facility Shanghai Advanced Research InstituteChinese Academy of SciencesShanghai 201204China School of Materials Science and Engineering Tsinghua UniversityBeijing 100084China

High pressure die casting(HPDC)AlSi10Mn Mg alloy castings are widely used in the automobile *** can optimize the mechanical properties of castings through heat treatment,while the release of thermal stress arouses the deformation of large integrated ***,the development of non-heat treatment Al alloys is becoming the hot *** addition,HPDC contains externally solidified crystals(ESCs),which are detrimental to the mechanical properties of *** achieve high strength and toughness of non-heat treatment die-casting Al-Si alloy,we used AlSi9Mn alloy as matrix with the introduction of Zr,Ti,Nb,and *** influences on ESCs and mechanical properties were systematically investigated through three-dimensional reconstruction and thermodynamic *** results reveal that the addition of Ti increased ESCs'size and porosity,while the introduction of Nb refined ESCs and decreased ***,large-sized Al_3(Zr,Ti)phases formed and degraded the mechanical *** introduction of Ce resulted in the poisoning effect and reduced mechanical properties.

关键词： aluminium alloy high-pressure die-casting externally solidified crystals non-heat treatment

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呼吸道合胞病毒核蛋白检测抗体的筛选与鉴定

中华微生物学和免疫学杂志 2018年第3期38卷 161-168页

作者：林雪张伟赵敏郑子峥夏宁邵厦门大学、分子疫苗学与分子诊断学国家重点实验室、国家传染病诊断试剂与疫苗工程技术研究中心 361102 厦门大学公共卫生学院 361102 厦门大学生命科学学院 361102

目的筛选识别呼吸道合胞病毒（respiratory syncytial virus，RSV）核蛋白（N）的单克隆抗体，并对其性质进行鉴定。方法使用大肠杆菌表达系统原核表达重组N蛋白，蛋白质纯化后免疫BALB/c小鼠。通过脾脏免疫、融合获得杂交瘤细胞，结合... 详细信息

目的筛选识别呼吸道合胞病毒（respiratory syncytial virus，RSV）核蛋白（N）的单克隆抗体，并对其性质进行鉴定。方法使用大肠杆菌表达系统原核表达重组N蛋白，蛋白质纯化后免疫BALB/c小鼠。通过脾脏免疫、融合获得杂交瘤细胞，结合有限稀释法亚克隆细胞株，间接酶联免疫吸附试验（ELISA）对单克隆抗体株进行筛选，并通过蛋白质免疫印迹法（Western blot）、ELISA和间接免疫荧光技术对最终筛选的单克隆抗体进行性质鉴定，根据正交试验的结果挑选捕获能力和检测效果较好的抗体进行诊断试剂的初步调试。结果成功构建N-pTO-T7原核表达载体，并成功表达、纯化得到高纯度重组N蛋白用于小鼠免疫，最终筛选获得24株N蛋白的单克隆抗体，24株抗体均具有较好的ELISA反应性，5A2等8株抗体有较好的Western blot检测特性，11H8等13株抗体可用于间接免疫荧光检测，并获得能够实现在病毒上检测N蛋白的优选配对12F2/11H8-HRP和12F2/15A8-HRP。结论本研究成功筛选出可用于N蛋白特异性检测的单克隆抗体及抗体配对，为RSV感染机制的研究及诊断试剂的研发提供实验资料。

关键词：呼吸道合胞病毒 N蛋白单克隆抗体

湖北巴戟天对D-半乳糖所致衰老模型小鼠的抗衰老作用

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中国医院药学杂志 2017年第17期37卷 1661-1666页

作者：边凌云范围梅雪伟张鹏华中科技大学同济药学院湖北省天然药物与资源评价重点实验室湖北武汉430030

目的:研究湖北巴戟天是否对D-半乳糖所致衰老模型小鼠具有抗衰老作用。方法:60只昆明种雄性小鼠随机分成6组,分别为正常组,模型组,维生素E(VitE)阳性对照组(100 mg·kg^(-1)·d^(-1))和湖北巴戟天醇提物低、中、高3个剂量组(15,30,45 mg... 详细信息

目的:研究湖北巴戟天是否对D-半乳糖所致衰老模型小鼠具有抗衰老作用。方法:60只昆明种雄性小鼠随机分成6组,分别为正常组,模型组,维生素E(VitE)阳性对照组(100 mg·kg^(-1)·d^(-1))和湖北巴戟天醇提物低、中、高3个剂量组(15,30,45 mg·kg^(-1)·d^(-1))。除正常组颈背部皮下注射等体积生理盐水外,其余各组颈背部皮下注射D-半乳糖(150 mg·kg^(-1)·d^(-1))建立衰老模型,为期60 d。统计各组小鼠在跳台和Morris水迷宫实验中错误次数或穿越次数,计算各组小鼠的脏器指数,测量血清及脑组织中SOD、CAT、GSH-Px活力和MDA含量,并测定各组小鼠脑细胞凋亡指数。结果:湖北巴戟天醇提物3个剂量组均能减少跳台错误次数(P<0.05或P<0.01),增加水迷宫穿越次数(P<0.05或P<0.01);升高小鼠血清及脑组织中SOD、CAT、GSH-Px活力(P<0.01),降低MDA含量(P<0.05);提高衰老小鼠脑、胸腺、脾脏和睾丸及附睾指数(P<0.05或P<0.01),减少细胞凋亡(P<0.01),呈剂量依赖性。结论:湖北巴戟天对D-半乳糖所致衰老模型小鼠具有较好的抗衰老作用。

关键词：湖北巴戟天 D-半乳糖抗衰老作用细胞凋亡指数

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某钢塔结构优化设计

建筑结构 2017年第3期47卷 97-99页

作者：钟志宏薛微张兵北京弘石嘉业建筑设计有限公司北京100600

西北地区某高层办公楼顶造型塔,在设计和施工过程中遇到了实际困难,在充分理解建筑造型创意的同时,巧妙运用力学原理、工程安装构造细节,在保证安全的前提下,对钢塔进行结构优化,最终采用的优化方案仅用了原钢塔方案1/20的钢材,达到了... 详细信息

西北地区某高层办公楼顶造型塔,在设计和施工过程中遇到了实际困难,在充分理解建筑造型创意的同时,巧妙运用力学原理、工程安装构造细节,在保证安全的前提下,对钢塔进行结构优化,最终采用的优化方案仅用了原钢塔方案1/20的钢材,达到了很好的优化效果。

关键词：钢结构往复作用高空安装工期成本