检索结果-南通市图书馆

Protective effect of hydrogen-rich saline on ischemia/reperfusion injury in rat skin flap

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2013年第5期14卷 382-391页

作者： Ling ZHAO You-bin WANG Shi-rui QIN xue-mei ma xue-jun SUN Ming-lian WANG Ru-gang ZHONG College of Life Science and Bioengineering Beijing University of Technology Peking Union Medical College Hospital Department of Diving Medicine Faculty of Naval Medicinethe Second Military Medical University

Objective: Skin damage induced by ischemia/reperfusion (I/R) is a multifactorial process that often occurs in plastic surgery. The mechanisms of I/R injury include hypoxia, inflammation, and oxidative damage. Hydrogen gas has been reported to alleviate cerebral I/R injury by acting as a free radical scavenger. Here, we assessed the protective effect of hydrogen-rich saline (HRS) on skin flap I/R injury. Methods: Abdominal skin flaps of rats were elevated and ischemia was induced for 3 h; subsequently, HRS or physiological saline was administered intraperitoneally 10 min before reperfusion. On postoperative Day 5, flap survival, blood perfusion, the accumulation of reactive oxygen species (ROS), and levels of cytokines were evaluated. Histological examinations were performed to assess inflammatory cell infiltration. Results: Skin flap survival and blood flow perfusion were improved by HRS relative to the controls. The production of malondialdehyde (MDA), an indicator of lipid peroxidation, was markedly reduced. A multiplex cytokine assay revealed that HRS reduced the elevation in the levels of inflammatory cytokines, chemokines and growth factors, with the exception of RANTES (regulated on activation, normal T-cell expressed and secreted) growth factor. HRS treatment also reduced inflammatory cell infiltration induced by I/R injury. Conclusions: Our findings suggest that HRS mitigates I/R injury by decreasing inflammation and, therefore, has the potential for application as a therapy for improving skin flap survival.

关键词： Hydrogen-rich saline Ischemia/reperfusion Skin flaps Oxidative damage Inflammation

Association of the macrophage migration inhibitory factor promoter polymorphisms with benign lymphoepithelial lesion of lacrimal gland

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

International Journal of Ophthalmology(English edition) 2017年第8期10卷 1229-1232页

作者： Qin-Jian Li Peng-Xiang Zhao Xu-Juan Zhang Yang Yi Dan-Ying Cheng Jian-Min ma xue-mei ma College of Life Science and Bio-engineering Beijing Universityof Technology Beijing 100124 China Institute of Infectious Diseases Beijing Ditan HospitalCapital Medical University Beijing 100015 China Beijing Tongren Eye Center Beijing Tongren HospitalCapital Medical University Beijing Ophthalmology &VisionScience Key Lab Beijing 100730 China

AIM： To identify the association of the macrophage migration inhibitory factor （MIF） gene polymorphism with the susceptibility of benign lymphoepithelial lesions （BLEL） of the lacrimal gland. METHODS： A total of 40 BLEL of lacrimal gland cases were matched with 40 healthy subjects （HS）. Extraction the plasma and whole blood DNA of patients of lacrimal gland BLEL and HS. Elisa and polymerase chain reaction was used to determine in plasma contents of MIF and MIF gene SNP-173G〉C and STR -794 CATT（8） polymorphism, respectively. RESULTS： The MIF levels in plasma were significantly higher in patients with lacrimal gland *** versus HS （P〈0.001）. The -173 G〉C MIF polymorphism was significantly associated with lacrimal gland BLEL, with a significantly higher frequency of the C allele in lacrimal gland BLEL patients compared with HS （OR=2.38, 95% C1=1.07-5.31, P=0.032）, and the -173 C/x is more frequent in patients than in HS, P=0.037. Besides, we found that the carriage rate of the MIF -173C/x is associated with higher plasma levels of MIF in the BLEI. of lacrimal gland. CONCLUSION： MIF -173G/C variants play an insidious role in susceptibility of BLEL of lacrimal gland. Otherwise,there is no statistically significant correlation exists between MIF-794 CATT （） and BLEL of lacrimal gland.

关键词： benign lymphoepithelial lesion lacrimal gland macrophage migration inhibitory factor gene polymorphism

Association analysis of BclI with benign lymphoepithelial lesions of the lacrimal gland and glucocorticoids resistance

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

International Journal of Ophthalmology(English edition) 2023年第11期16卷 1734-1745页

作者： Xu-Juan Zhang Peng-Xiang Zhao Ming-Shen ma Hao Wu Rui Liu Hui Wang Meng-Yu Liu Fei Xie xue-mei ma Faculty of Environment and Life Beijing University of TechnologyBeijing 100124China Beijing International Science and Technology Cooperation Base of Antivirus Drug Beijing 100124China Beijing Tongren Hospital Capital Medical UniversityBeijing 100730China State Key Laboratory of Military Stomatology&National Clinical Research Center for Oral Diseases&Shaanxi International Joint Research Center for Oral Diseases Department of General Dentistry and EmergencySchool of StomatologyAir Force MedicalXi’an 710032Shaanxi ProvinceChina

AIM:To evaluate the relationship between gene polymorphism(BclI,ER22/23EK,N363S)and the occurrence,progression and sensitivity to glucocorticoid of lacrimal gland benign lymphoepithelial lesion(LGBLEL).METHODS:Clinical peripheral blood samples of 52 LGBLEL patients and 10 normal volunteers were collected for DNA extraction and polymerase chain reaction sequencing to analyze single nucleotide polymorphism(SNP)*** lacrimal tissues of LGBLEL were surgically removed and made into paraffin sections for subsequent hematoxylin-eosin(HE)and masson staining *** duration of disease and hormone use of LGBLEL patients from diagnosis to surgery were also *** Meta-analysis follows PRISma guidelines to conducted a systematic review of human studies investigating the relationship between the NR3C1 BclI polymorphism and glucocorticoids(GCs)***:There was no association between ER22/23EK or N363S and the occurrence of LGBLEL or GCs sensitivity(P>0.05);BclI GC genotype was closely related to GCs resistance(P=0.03)as is the minor allele C(P=0.0017).The HE staining and masson staining showed that the GC genotype of BclI remarkably slowed down the disease progression and reduced fibrosis(Pmay be more sensitive to GCs and associated with lower fibrosis and slower disease *** results may guide the clinical treatment strategy for the LGBLEL patients.

关键词： lacrimal gland benign lymphoepithelial lesion Bcll single nucleotide polymorphisms glucocorticoids resistance fibrosis

CDX2 Inhibits Invasion and Migration of Gastric Cancer Cells by Phosphatase and Tensin Homologue Deleted from Chromosome 10/Akt Signaling Pathway

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Medical Journal 2015年第8期128卷 1065-1071页

作者： Yong-Qiang Liu Zhi-Gang Bai xue-mei ma Zhong-Tao Zhang Department of General Surgery Beijing Friendship Hospital Capital Medical University Beijing 100050 China

Background： Gastric cancer （GC） is one of the most prevalent malignancies in the world today, with a high mortality rate. CDX2 is a Drosophila caudal-related homeobox transcription factor that plays an important role in GC. Phosphatase and tensin homologue deleted from chromosome 10 （PTEN） is an important tumor suppressor which is widely expressed in normal human tissues. The aim of the study was to determine the relationship and mechanism between CDX2 and PTEN in invasion and migration of GC ***： pcDNA3-CDX2 plasr^lids were transfected into MGC-803 cells to up-regulate CDX2 protein, and small interfering RNA-CDX2 was transfected to down-regulate CDX2. The influence of CDX2 or PTEN on cell migration and invasion was measured by invasion, migration and wound healing assays. Western blotting assay and immunofluorescence were used to detect the expression ofCDX2, PTEN, phosphorylation ofAkt, E-cadherin and N-cadherin. Statistical significance was determined by one-way analysis of ***： The results showed that CDX2 reduced the migration and invasion of GC cells （P 〈 0.05）, and inhibited the activity of Akt through down-regulating PTEN expression （P 〈 0.05）. CDX2 also restrained epithelial-mesenchymal transition of GC ***： CDX2 inhibited invasion andmigration of GC cells by PTEN/Akt signaling pathway, and that may be used for potential therapeutic target.

关键词： CDX2 Epithelial-mesenchymal Transition Gastric Cancer PTEN

IgG4 and IgE co-positive group found in idiopathic orbital inflammatory disease

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

International Journal of Ophthalmology(English edition) 2018年第1期11卷 36-42页

作者： Peng-Xiang Zhao Yao mawulikplimi Adzavon Jian-Min ma Lei Shang Dan-Ying Chen Fei Xie Meng-Yu Liu Xin Zhang Bao-Bei Lyu Ming-Zi Zhang Lin-Qi Yang xue-mei ma College of Life Science and Bio-engineering Beijing University of Technology Beijing Ophthalmology & Vision Science Key Lab Beijing Tongren Eye Center Beijing Tongren Hospital Capital Medical University Institute of Infectious Diseases Beijing Ditan Hospital Capital Medical University Department of Plastic Surgery Peking Union Medical College Hospital

AIM： To reveal the cytokines involved in idiopathic orbital inflammatory disease（IOID） and the relationship between Th17 cells, IgE and IOID ***： Whole blood samples were processed immediately after collection and serological IgG4, IgG, and IgE antibodies were tested using ELISA. IOID and orbital cavernous hemangioma（CH） tissue samples underwent Bio-Plex multiplex cytokine detection. Hematoxylin-Eosin（HE） staining of all paraffin samples suggested the histological features of IOIDs, and expressions of IgG4 and IL-17 A in affected tissues were detected by immunohistochemistry. RESULTS： Among 40 IOID plasma samples, 52.5%（21/40） were positive for IgG4 and 25%（10/40） were positive for IgE. Overlapped IgG4 or IgE positive samples accounted for 22.5%（9/40）. Therefore, IOID samples were separated into three groups. The IgE＋/IgG4＋ group had a relevantly lower level of pro-inflammatory cytokine expression. IL-4（Th2 cell related）, IL-10 and TGF-β1（Treg cell immunity related） were elevated in all three groups. Some of the Th17 cell related cytokines（i.e. IL-17 A/F, IL-25, IL-23, and IL-33） displayed higher expression levels in the IgE-/IgG4-group compared to the other two ***： We discovered an IgG4-IgE co-positive group as well as Th17 cell immune involvement in IgG4-IgE co-negative subgtroup in IOID for the first time. The pathogenesis of IOID could differ from different subgroups according to the IgG4 and IgE detection. Therefore, we recommend that, Treatment stratagy should be made according to the clinical assessment of IgG4-IgE and Th17 profile detection.

关键词： idiopathic orbital inflammatory disease IgE IgG4 Th17

Risk factors for esophagogastric variceal-related poor outcomes in primary biliary cholangitis:A prospective cohort study

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

iLIVER 2024年第1期3卷 30-37页

作者： Lin Han Huan Xie xue-mei ma Xiao-Long Lu Jun Zhao Qing-Sheng Liang Zheng-Sheng Zou Jing-Feng Bi Bo Jin Ying Sun Senior Department of Hepatology The Fifth Medical Center of PLA General HospitalBeijing 100039China Senior Department of Infectious Diseases Medicine The Fifth Medical Center of PLA General HospitalBeijing 100039China Graduate School Medical School of Chinese PLABeijing 100039China

Background and aims:Esophagogastric varices(EGV)are common complications of primary biliary cholangitis(PBC).We examined the risk factors for variceal bleeding-related liver transplantation(LT)or ***:This prospective observational cohort study involved PBC in our hospital from 1 January 2005 to 1 January *** clinical endpoints were variceal bleeding-related LT and *** analysis was performed using the Kaplan-meier estimate,cox regression analysis was performed to investigate risk ***:PBC with EGV had significantly shorter survival than those without(p?0.002).Endoscopic prophylaxis significantly improved poor outcomes in PBC with EGV(pmal(ULN),international normalized ratio(INR)of>1.2ULN at baseline,total bilirubin of>1.2ULN,aspartate aminotransferase(AST)of>2.3ULN after 1 year of ursodeoxycholic acid(UDCA)treatment,non-biochemical responders according to the Paris criteria,and no history of endoscopic *** PBC without EGV,risk factors included AST of>2.3ULN,INR of>1.2ULN at baseline,CHE of***:This study provides evidence that AST,INR and CHE are major risk factors for variceal bleedingrelated poor outcomes in *** PBC with EGV,a good biochemical response to UDCA and endoscopic prophylaxis may improve *** findings can aid for guiding initial PBC risk stratification and screening endoscopy in patients without EGV.

关键词： Primary biliary cholangitis Esophagogastric varices Ursodeoxycholic acid Liver transplant-free survival Endoscopic therapy Risk factors

Effect of hydrogen intervention on refractory wounds after radiotherapy:A case report

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Clinical Cases 2022年第21期10卷 7545-7552页

作者： Peng-Xiang Zhao Rui-Liu Luo Zheng Dang You-Bin Wang Xu-Juan Zhang Zi-Yi Liu Xiao-Hu Wen Meng-Yu Liu Ming-Zi Zhang Yao mawulikplimi Adzavon xue-mei ma Faculty of Environment and Life Science Beijing International Science and TechnologyCooperation Base of Antivirus DrugBeijing University of TechnologyBeijing 100124China Department of Plastic Surgery Peking Union Medical College HospitalBeijing 100032China

BACKGROUND Patients with keloids who receive radiotherapy(RT)after surgery can develop refractory wounds that cannot be healed by the patient's own repair *** chronic wounds are uneven and complex due to persistent abscess and *** external intervention,they can easily result in local tissue necrosis or,in severe cases,large area tissue resection,amputation,and even *** SUMmaRY This article describes the use of hydrogen to treat a 42-year-old female patient with a chronic wound on her left *** patient had a skin graft that involved implanting a dilator under the skin of her left shoulder,and then transferring excess skin from her shoulder onto scar tissue on her *** skin grafting was followed by two rounds of RT,after which the shoulder wound had difficulty *** six months,the patient was treated with 2 h of hydrogen inhalation(HI)therapy per day,in addition to application of sterile gauze on the wound and periodic *** also performed one deep,large,sharp debridement to enlarge the wound *** wound healed completely within 6 mo of beginning the HI *** After HI therapy,the patient showed superior progress in reepithelialization and wound repair,with eventual wound closure in 6 mo,in comparison with the previous failures of hyperbaric oxygen and recombinant bovine basic fibroblast growth factor *** work showed that HI therapy could be a new strategy for wound healing that is cleaner,more convenient,and less expensive than other therapies,as well as easily accessible for further application in clinical wound care.

关键词： Hydrogen Inhalation therapy Wound healing Reepithelialization Refractory wounds Case report

Effect of increased hepatic platelet activating factor and its receptor portal hypertension in CCl_4-induced liver cirrhosis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2006年第5期12卷 709-715页

作者： Yong-Ping Yang xue-mei ma Chun-Ping Wang Jun Han Yin-Ying Lu Yi Xiang Shu-Hui Su Yong-Yi Feng Department of Gastroenterology 302 Hospital ofPLA Beijing 100039 China

AIM： To evaluate the changes in hepatic platelet activating factor （PAF） and its receptors and their effect on portal pressure of cirrhotic rats induced by CCh. METHODS： A model of liver cirrhosis was replicated in rats by intra-peritoneal injection of CCh for 8 wk. We determined the effect of hepatic PAF and its receptor level on portal and arterial pressure by EIA, saturation binding and RT-PCR technique. RESULTS： Compared to control rats, cirrhotic rats had higher hepatic PAF levels and output as well as higher plasma PAF levels （P〈0.01, P〈0.01, P〈0.05, respectively）. Both hepatic PAF receptor mRNA levels and PAF binding were nearly 3-fold greater in cirrhotic rats （P〈0.01）. Portal injection of PAF （1 g/kg WT） increased the portal pressure by 22% and 33% in control and cirrhotic rats, respectively. In contrast, the arterial pressure was decreased in the both groups （54% in control rats and 42% in cirrhotic rats）. Injection of the PAF antagonist BN52021 （5 mg/kg WT） decreased the portal pressure by 16% in cirrhotic rats but had no effect in the control rats. CONCLUSION： The upregulation of the PAF system contributes to hepatic hemodynamic and metabolic abnormalities in drrhosis, and the increased release of PAF into the circulation has impacts on the systemic hemodynamics.

关键词： Platelet activating factor PAF receptors Endothelin Portal hypertension Cirrhosis

Synergistic effect of hyperbaric oxygen preconditioning and hydrogen-rich saline in ameliorating rat flap ischemia/reperfusion injury

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Plastic and Aesthetic Research 2015年第1期2卷 332-339页

作者： Yi-Ding Xiao Yun-Qi Liu Ming-Zi Zhang You-Bin Wang Yi-Fang Liu xue-mei ma Department of Plastic Surgery Peking Union Medical College HospitalDongcheng 100730BeijingChina College of Life Science and Bioengineering Beijing University of TechnologyChaoyang 100124BeijingChina

Aim:This study was conducted to evaluate the synergistic effects of hyperbaric oxygen(HBO)preconditioning and hydrogen-rich saline(HRS)treatment on skin flap survival and apoptosis in a rat ischemia/reperfusion(IR)skin flap ***:male Sprague-Dawley rats were randomly divided into five groups:one sham surgery group(sham group)and four surgery groups(IR group,HBO group,HRS group,and HBO+HRS group).An extended epigastric adipocutaneous flap(6 cm×9 cm)was raised over the abdomen in each animal of all five *** last four groups underwent 6 h of IR management and were treated,respectively,with normal saline,HBO,HRS(HRS,0.8 mmol/L),or a combined approach(HBO and HRS).On the 3rd postoperative day,flap survival rate and perfusion condition,apoptotic index,caspase-3 activity,protein expression of pASK1 and Bcl-2/Bax ratio,and Bcl-2 messenger RNA(mRNA)expression were ***:Prior studies have shown the protective effects of HBO and HRS,both of which have been associated with an increase in flap *** to the IR group,the flaps in the HBO,HRS,and HBO+HRS groups showed better perfusion and a larger survival area with a low number of apoptotic cells,low caspase-3 activity and pASK1 expression,and a high Bcl-2/Bax ratio and Bcl-2 mRNA *** these groups,the HBO+HRS group showed the best flap ***:Both HBO and HRS treatments increase the rate of flap survival,while the synergistic application of HBO and HRS showed a higher survival rate as compared to individual treatments of *** potential regulation of apoptosis with the use of these two modalities may improve skin flap survival.

关键词： Apoptosis hydrogen‑rich saline hyperbaric oxygen ischemia/reperfusion injury skin flaps