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BSA-lysozyme coated NaCa_(2)HSi_(3)O_(9)nanorods on titanium for cytocompatibility and antibacterial activity

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Journal of Materials Science & Technology 2021年第29期88卷 240-249页

作者： Yang xue jun chen Lan zhang Yong Han State-key Laboratory for Mechanical Behavior of Materials Xi'an Jiaotong UniversityXi'an 710049China Department of Osteology Xi'an People’s Hospital(Xi'an No.4 Hospital)Xi'an 710100China Department of Osteology Tangdu HospitalFourth Military Medical UniversityXi'an 710038China

Extracellular supplement of Ca and Si accelerates osteogenisis and angiogenisis during bone ***-based ceramics are biodegradable and can release Ca and Si ions ***,locally high alkalinity induced by excessively rapid degradation limits their *** this paper,NaCa_(2)HSi_(3)O_(9)(NCS)nanorods were fabricated on Ti by micro-arc oxidation and hydrothermal treatment;protein layers consisted of different rations of bovine serum albumin(BSA)and lysozyme(LYS)were prepared on NCS nanorods by spinning coating and thermal *** microstructure,ion releasing and wettability of coatings were observed;osteoblast and endotheliocyte behaviors,as well as bacteria response in vitro,were *** protein layer did not change microstructure and phase composition of nanorods but acted as a barrier isolating nanorods from contacting medium,and it results in slowing down the degradation of *** reduced increase of pH value,released Ca and Si ions and incorporation of LYS in BSA protein layer gave NCS nanorods improved MC3 T3-E1 and HUVECs response,compared with ***,the protein layers except pure LYS endowed nanorods with a highly antibacterial effect against staphylococcus aureus and escherichia coli simultaneously,indicating the key role of BSA in resisting antibacterial *** nanorods with BSA and LYS composited protein layers should be a potential coating applied on Ti implants for increasing osseointegration.

关键词： NaCa_(2)HSi_(3)O_(9)nanorods Bovine serum albumin Lysozyme Antibacterial activity Cytocompatibility

Self-Healing of Stone–Wales Defects in Boron Nitride Monolayer by Irradiation:Ab Initio Molecular Dynamics Simulations

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Chinese Physics Letters 2013年第10期30卷 73-76页

作者： xue Shu-Wen chen Jian zhang jun Department of Physics Zhanjiang Normal CollegeZhanjiang 524048

We show an effective method of healing the Stone–Wales(SW)defects through low energy electron irradiation using ab initio molecular dynamics *** SW defects can be healed by irradiation through bond *** the healing energy shows an anisotropic behavior,it is lower than the displacement threshold *** healing of the SW defect through electron irradiation can be effectively used in other sp2-bonded materials.

关键词： energy Wales healing

Reprogrammed CRISPR-Cas9 targeting the conserved regions of HPV6/11 E7 genes inhibits proliferation and induces apoptosis in E7-transformed keratinocytes

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Asian Journal of Andrology 2016年第3期18卷 475-479,I0011,I0012页

作者： Yu-chen Liu Zhi-Ming Cai xue-jun zhang Institute of Dermatology Department of Dermatology The First Affiliated Hospital Anhui Medical University Hefei China Key Laboratory of Dermatology Ministry of Education State Key-Laboratory of Dermatology Incubation Center Department of Dermatology Anhui Medical University Hefei China Key Laboratory of Medical Reprogramming Technology Department of Urology The Genitourinary Institution of Shenzhen University Shenzhen Second People's Hospital The First Affiliated Hospitalof Shenzhen University Shenzhen China.

The persistence infection of low-risk type （type 6 or type 11） of human papillomavirus （HPV） is the main cause of genital warts. Given the high rate of recurrence after treatment, the use of a new molecular agent is certain to be of value. The aim of this study was to achieve targeted inactivation of viral E7 gene in keratinocytes using the reprogrammed clustered regularly interspaced short palindromic repeats （CRISPR）/CRISPR-associated （Cas） 9 system. To accomplish this, a universal CRISPR-Cas9 system for targeting both HPV6/11 E7 genes was constructed by using a dual guide RNA vector. After transfection of the vector into E7-transfromed keratinocytes, the expression level of E7 protein was measured using western-blot analysis and the sequence of the E7 gene was determined using Sanger sequencing. Cell proliferation was analyzed by CCK-8 assay, and cell apoptosis was evaluated by Hoechst 33258 staining, flow cytometry analysis and ELISA assay. The results indicated that both HPV6/11 E7 genes can be inactivated by the single CRISPR-Cas9 system. Furthermore, silencing of E7 led to inhibition of cell proliferation and induction of apoptosis in E7-transfromed keratinocytes but not in normal keratinocytes. Our data suggested that the reprogrammed CRISPR-Cas9 system has the potential for the development of an adjuvant therapy for genital warts.

关键词： apoptosis CRISPR-Cas9 HPV E7 proliferation

Investigation of the first quasi-rectangular metro tunnel constructed by the 0-θ method

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Frontiers of Structural and Civil Engineering 2023年第11期17卷 1707-1722页

作者： Peinan LI xue LIU Xi JIANG xuehui zhang jun WU Peixin chen College of Environmental Science and Engineering Donghua UniversityShanghai 201620China Department of Civil and Environmental Engineering University of TennesseeKnoxvilleTN 37996USA Department of Geoscience and Engineering Delft University of TechnologyDelft 2628The Netherlands College of Urban Railway Transportation Shanghai University of Engineering ScienceShanghai 201620China Shanghai Tunnel Engineering Co..Ltd. Shanghai 200032China

Quasi-rectangular shield tunneling is a cutting-edge trenchless method for constructing metro tunnels with double tubes,owing to its advantages in saving underground space and reducing ground ***,the conventional quasi-rectangular shield tunneling method is not applicable when constructing a tunnel without a center pillar,such as a scissor crossover section of a metro ***,the O-0 tunneling method,which combines the quasirectangular shield and pipe jacking methods,was investigated in this study to solve the aforementioned construction *** study presents a case study of the Sijiqing Station of the Hangzhou Metro Line 9 in China,in which the O-0 method was first proposed and *** techniques such as switching between two types of tunneling modes and the tunneling process control in complex construction environments were *** results demonstrated that the O-0 method can address the technical challenges presented by the post-transition line with a high curvature and a scissors crossover *** addition,the adoption of the 0-0 method ensured that the transformation between shield tunneling and pipe jacking was safe and *** ground settlement monitoring results demonstrated that the disturbance to the surrounding environment can be limited to a safe *** case study contributes to the construction technology for a metro tunnel containing both post-transition lines with a small turning radius and a scissors crossover line.A practical construction experience and theoretical guidance were provided in this study,which are of significance for both the industry and academia.

关键词： quasi-rectangular tunnel O-0 method pipe jacking shield tunneling underground space

Experimental and numerical investigation of the impacts of rotor tip-rake on excitation forces of pump-jet propulsors

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Journal of Hydrodynamics 2024年第1期36卷 142-157页

作者： xue-qin Ji Xiao-song zhang chen-jun Yang Xiao-qian Dong State Key Laboratory of Ocean Engineering Marine Numerical Experiment CentreSchool of Naval ArchitectureOcean and Civil EngineeringShanghai Jiao Tong UniversityShanghai200240China CSSC Shanghai Marine Energy Saving Technology Co. Ltd.Shanghai200011China China Ship Scientific Research Center Shanghai Branch Shanghai200011China

The tip-clearance flow in a pump-jet propulsor exerts great impacts on the fluctuating pressures and resultant unsteady forces,which are important sources of structural vibrations and radiated noise *** blade geometry close to the tip is an important factor determining the vortex strength in the tip-clearance *** the open-water condition,the effects of raking the rotor tips on the duct-surface fluctuating pressures and the resultant unsteady forces acting on different components of the propulsor are investigated via physical model experiments and the numerical solution of Reynolds-averaged Navier-Stokes(RANS)equations coupled with the SST k-ωturbulence *** measured and simulated results of hydrodynamic pressures are consistent to each other,and the simulated flows help better understand why the fluctuating pressures change with the tip *** strong fluctuations of duct-surface pressures are caused by intensive tip separation *** duct-surface pressure fluctuations are effectively reduced by using the rake distribution near the tip towards blade back side and,for the combination of the five-bladed rotor and the seven-bladed stator,the resultant unsteady horizontal(and vertical)forces acting on the duct and stator are also reduced;while increasing rake leads to negative effect on pressure fluctuations and unsteady horizontal(and vertical)forces acting on all the components of the propulsor.

关键词： Pump-jet tip-clearance tip-rake fluctuating pressures excitation forces

Effect of mixing temperature on microstructure of an Al-Si alloy prepared by controlled diffusion solidification

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China Foundry 2023年第3期20卷 241-252页

作者： Han xue Ti-jun chen xue-zheng zhang Guang-li Bi Ying Ma Ren-guo Guan State Key Laboratory of Advanced Processing and Recycling of Nonferrous Metals Lanzhou University of TechnologyLanzhou 730050China School of Materials Science and Engineering Dalian Jiaotong UniversityDalian 116028LiaoningChina

The effects of mixing temperature,i.e.,the temperatures of two precursor melts(pure Al and Al-12Si),on the temperature and solute fields of resultant mixture,the nucleation and growth,and the size and morphology of primary grains during controlled diffusion solidification(CDS) of Al-8Si alloy were investigated by using simulation and *** results indicate that a lower mixing temperature is helpful for achieving more supercooled microscale Al-rich pockets in the mixture,and increasing the width and supercooling degree of supercooling zone in the Al-rich pockets,and thus,the nucleation *** nuclei grow up in nondendritic mode,resulting in spheroidal,at least,nondendritic *** a successful CDS,the superheat degrees of the two precursor melts should be limited within several degrees,and it is not necessary to extra stipulate the superheat degree of target alloy melt(Al-8Si) when the requirement about Gibbs energies of the three melts is *** observation on casting microstructures shows that the employed simulation and calculation processes are reasonable and the achieved results are reliable.

关键词： controlled diffusion solidification Al-Si alloy nucleation and growth supercooling nondendritic grains

Dynamic Subcarrier Assignment for OFDM-PON Network Based on RSOA for ACCORDANCE

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China Communications 2014年第6期11卷 81-88页

作者： LIU Zhuo zhang Min JIANG jun ZHAN Yueying chen xue State Key Lab. of Information Photonics and Optical Communications Beijing Univ. of Posts & Telecom.10 Xitucheng Road

In this paper, we overview the principle of Orthogonal Frequency Division Multiplexing Passive Optical Network （OFDM-PON） systems, with a particular focus on upstream architectures capable of achieving 10Gbit/s colorless upstream transmission using Reflective Semiconductor Optical Amplifier （RSOA）. We propose an architecture of RSOA based OFDM-PON which can achieve 10Gbit/s upstream transmission over a single wavelength. A novel Dynamic Subcarrier Assignment （DSA） algorithm is also proposed to support my architecture, namely Service based Polling in Pipeline （SPP） dynamic subcarrier algorithm. A simulation was conducted to study the performance of SPP algorithm. Compared with the traditional dynamic bandwidth allocation algorithms, service based polling meets the quality of in pipeline algorithm service requirements excellently, and adapts orthogonal frequency division multiplexing passive optical network better with higher bandwidth efficiency and lower algorithm complexity.

关键词： OFDM-PON RSOA DBA QoS SPP

Community analysis of ammonia and nitrite oxidizers in start-up of aerobic granular sludge reactor

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Journal of Environmental Sciences 2007年第8期19卷 996-1002页

作者： WANG Feng XIA Si-qing LIU Yi chen xue-song zhang jun State Key Laboratory of PoUution Control and Resource Reuse School of Environmental Science and Engineering Tongji University Shanghai 200092 China. College of Biology and Environment Engineering Zhejiang Shuren University Hangzhou 310015 China Medical School Tongji University Shanghai 200092 China

A lab-scale sequencing batch reactor （SBR） was set-up and the aerobic granular sludge was successfully incubated using anaerobic granular sludge as seed sludge. Nitrogen was partially removed by simultaneous nitrification and denitrification （SND） via nitrite with free ammonia （FA） of about 10 mg/L. The denaturing gradient gel electrophoresis （DGGE） method was used to investigate community structure of α-Proteobacteria, β-Proteobacteria, ammonia oxidizing bacteria （AOB）, and Nitrospira populations during start-up. The population sizes of bacteria, AOB and Nitrospira were examined using real-time PCR method. The analysis of community structure and Shannon index showed that stable structure of AOB population was obtained at day 35, while the communities of α- Proteobacteria, β-Proteobacteria, and Nitrospira became stable after day 45. At stable stage, the average cell densities were 1.1× 10^12, 2.2×10^10 and 1.0×10^10 cells/L for bacteria, AOB and Nitrospira, respectively. The relationship between characteristics of nitrifying bacteria community and nitrogenous substrate utilization constant was discussed by calculating Pearson correlation. Certain correlation seemed to exist between population size, biodiversity, and degradation constant. And the influence of population size might be greater than that of biodiversity.

关键词： aerobic granular sludge simultaneous nitrification and denitrification nitrite accumulation denaturing gradient gel electrophoresis real-time PCR

Wave Attenuation Performance and the Influencing Factors of A Lower Arc-Plate Breakwater

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China Ocean Engineering 2020年第1期34卷 89-98页

作者： LI xue-yan WANG Qing YOU Zai-jin GUO Wei-jun zhang jun-bin ZHAN Chao zhang Zhi-chen WANG Li-xue LI Qin Coast Institute Ludong UniversityYantai 264025China Dalian Maritime University Dalian 116026China Institute of Sea-Crossing Engineering Ludong UniversityYantai 264024China College of Environmental Sciences and Engineering Dalian Maritime UniversityDalian 116026China

Comprehensive experimental and numerical studies have been undertaken to investigate wave energy dissipation performance and main influencing factors of a lower arc-plate breakwater. The numerical model, which considers nonlinear interactions between waves and the arc-plate breakwater, has been constructed by using the velocity wave- generating method, the volume of fluid (VOF) method and the finite volume method. The results show that the relative width, relative height and relative submergence of the breakwater are three main influencing factors and have significant influence on wave energy dissipation of the lower arc-plate open breakwater. The transmission coefficient is found to decrease with the increasing relative width, and the minimum transmission coefficient is 0.15 when the relative width is 0.45. The reflection coefficient is found to vary slightly with the relative width, and the maximum reflection coefficient is 0.53 when the relative width is 0.45. The transmission and reflection coefficients are shown to increase with the relative wave height for approximately 85% of the experimental tests when the relative width is 0.19 0.45. The transmission coefficients at relative submergences of 0.04, 0.02 and 0 are clearly shown to be greater than those at relative submergences of 0.02 and 0.04, while the reflection coefficient exhibits the opposite relationship. After the wave interacts with the lower arc-plate breakwater, the wave energy is mainly converted into transmission, reflection and dissipation energies. The wave attenuation performance is clearly weakened for waves with greater heights and longer periods.

关键词： arc-plate breakwater wave attenuation performance influencing factors VOF method