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Insights and reviews on battery lifetime prediction from research to practice

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Journal of Energy Chemistry 2024年第7期94卷 716-739页

作者： xudong qu Dapai Shi Jingyuan Zhao Manh-Kien Tran Zhenghong Wang Michael Fowler Yubo Lian Andrew FBurke Hubei Longzhong Laboratory Hubei University of Arts and ScienceXiangyang 441000HubeiChina Institute of Transportation Studies University of California DavisDavisCA 95616USA Department of Chemical Engineering University of WaterlooWaterlooOntario N2L 3G1Canada BYD Automotive Engineering Research Institute Shenzhen 518118GuangdongChina

The rising demand for energy storage solutions,especially in the electric vehicle and renewable energy sectors,highlights the importance of accurately predicting battery health to enhance their longevity and *** article comprehensively examines various methods used to forecast battery health,including physics-based models,empirical models,and equivalent circuit models,among *** delves into the promise of data-driven prognostics,utilizing both conventional machine learning and cuttingedge deep neural network *** advantages and limitations of hybrid models are thoroughly analyzed,with a focus on the benefits of integrating diverse data sources to improve prognostic *** practical case studies,the article showcases the effectiveness and flexibility of these *** also critically addresses the challenges encountered in applying battery health prognostics in realworld scenarios,such as issues of scalability,complexity,and data *** these challenges,the article underscores the emerging opportunities brought about by recent technological,academic,and research *** include the development of digital twin models for batteries,the use of data-centric AI and standardized benchmarking,the potential integration of blockchain technology for enhanced data security and transparency,and the synergy between edge and cloud computing to boost data analysis and *** primary goal of this article is to enrich the understanding of current battery health prognostic techniques and to inspire further research aimed at overcoming existing hurdles and tapping into new *** concludes with a visionary perspective on future research directions and potential developments in this evolving field,encouraging both researchers and practitioners to explore innovative solutions.

关键词： Batteries Lifetime Health Machine learning Deep learning Field Real world

Efficient editing DNA regions with high sequence identity in actinomycetal genomes by a CRISPR-Cas9 system

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Synthetic and Systems Biotechnology 2019年第2期4卷 86-91页

作者： Jingjun Mo Shuwen Wang Wan Zhang Chunyu Li Zixin Deng Lixin Zhang xudong qu Key Laboratory of Combinatorial Biosynthesis and Drug Discovery Ministry of EducationSchool of Pharmaceutical SciencesWuhan UniversityWuhanChina State Key Laboratory of Bioreactor Engineering East China University of Science and TechnologyShanghaiChina

Actinobacteria able to produce varieties of bioactive natural products have been long appreciated by the field of drug discovery and ***,a few of CRISPR/Cas9 systems bearing different types of replicons(pSG5 and pIJ101)were developed to efficiently edit their *** wide application in gene editing,their utility in editing challenging DNA regions *** sequence identity has not been *** this study,we confirmed that the widely used temperature-sensitive pSG5 replicon is indeed not suitable for editing modular polyketide synthase(PKS)genes due to causing unpredicted gene *** problem can be addressed by replacing the pSG5 with the segregationally unstable pIJ101 *** introducing a counterselection marker CodA,convenient cloning sites in the single guide RNAs(sgRNAs)and homologous template scaffolds,we developed a new CRISPR-Cas9 system *** system was successfully used to delete/replace erythromycin PKS and other biosynthetic genes in Saccharopolyspora erythraea and Streptomyces *** swapping the promoters of antB and antC with ermE and kasOp,we achieved a deacyl-antimycin hyper producer which produces a 9-fold higher yield than the original Streptomyces ***2110 *** results provide a robust and useful Cas9 tool for genetic studies in Actinobacteria.

关键词： Biosynthesis Polyketide synthase CRISPR/Cas9 Antimycin Actinobacteria

Puromycin A, B and C, cryptic nucleosides identified from Streptomyces alboniger NRRL B-1832 by PPtase-based activation

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Synthetic and Systems Biotechnology 2018年第1期3卷 76-80页

作者： Xiaoli Yan Benyin Zhang Wenya Tian Qi Dai Xiaoqin Zheng Ke Hu Xinxin Liu Zixin Deng xudong qu Key Laboratory of Combinatorial Biosynthesis and Drug Discovery Ministry of Education School of Pharmaceutical SciencesWuhan UniversityChina State Key Laboratory of Plateau Ecology and Agriculture Qinghai UniversityChina Jiangshu National Synergetic Innovation Center for Advanced Materials(SICAM) China

Natural product discovery is pivot for drug development,however,this endeavor is often challenged by the wide inactivation or silence of natural products biosynthetic *** recently developed a highly efficient approach to activate cryptic/silenced biosynthetic pathways through augmentation of the phosphopantetheinylation of carrier *** applying this approach in the Streptomyces alboniger NRRL B-1832,we herein identified three cryptic nucleosides products,including one known puromycin A and two new derivatives(puromycin B and C).The biosynthesis of these products doesn't require the involvement of carrier protein,indicating the phosphopantetheinyl transferase(PPtase)indeed plays a fundamental regulatory role in metabolites *** results demonstrate that the PPtasebased approach have a much broader effective scope than the previously assumed carrier proteininvolving pathways,which will benefit future natural products discovery and biosynthetic studies.

关键词： Puromycin Phosphopantetheinyl transferase Gene activation Streptomyces alboniger Genome mining

Structure-guided protein design of fluoroacetate dehalogenase for kinetic resolution of rac-2-bromobutyric acid

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Green Synthesis and Catalysis 2020年第1期1卷 60-65页

作者： Shu Wang Zhanbing Cheng Yanbing Xu Lu Yang Jian-Bo Wang Zhenhua Tian xudong qu Shanghai R&D Center Abiochem Co.Ltd.Shanghai 200241China State Key Laboratory of Microbial Metabolism School of Life Sciences and BiotechnologyShanghai Jiao Tong UniversityShanghai 200240China Key Laboratory of Phytochemistry R&D of Hunan Province and Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research(Ministry of Education)College of Chemistry and Chemical EngineeringHunan Normal UniversityChangsha 410081China

R-2-Bromobutyric acid is a very important intermediate for the synthesis of agrochemicals and *** of rac-2-bromobutyric acid(rac-2-BBA)provides a promising process for R-2-bromobutyric acid(R-2-BBA)*** fluoroacetate dehalogenase(FAcD)has been always studied in the defluorination *** found that FAcD RPA1163 showed detectable activity but no enantioselectivity towards *** iterative saturation mutagenesis(ISM)of FAcD RPA1163 resulted in a mutant H155V/W156R/Y219M,which catalyzed the kinetic resolution of rac-2-BBA to produce R-2-BBA with enhanced activity and enantioselectivity(99.3%ee).The high preference for S-2-bromobutyric acid(S-2-BBA)is of synthetic *** docking analysis indicated that the H155V/W156R/Y219M mutation reduced steric hindrance and broadened the halide *** is not only the steric hindrance but also the electrostatic environment that has an effect on the activity and enantioselectivity.

关键词： Fluoroacetate dehalogenase Semi-rational design Enzyme engineering Kinetic resolution 2-bromobutyric acid

Norditerpenoids biosynthesized by variediene synthase-associated P450 machinery along with modifications by the host cell Aspergillus oryzae

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Synthetic and Systems Biotechnology 2022年第4期7卷 1142-1147页

作者： Lan Jiang Kangjie Lv Guoliang Zhu Zhi Lin Xue Zhang Cuiping Xing Huanting Yang Weiyan Zhang Zhixin Wang Chengwei Liu xudong qu Tom Hsiang Lixin Zhang Xueting Liu State Key Laboratory of Bioreactor Engineering East China University of Science of TechnologyShanghaiChina State Key Laboratory of Microbial Metabolism and School of Life Sciences and Biotechnology Shanghai Jiao Tong UniversityShanghai200240China Key Laboratory of Saline-alkali Vegetation Ecology Restoration Ministry of EducationCollege of Life SciencesNortheast Forestry UniversityHarbinChina School of Environmental Sciences University of Guelph50 Stone Road EastGuelphOntarioCanada

The chemical diversity of terpenoids is typically established by terpene synthase-catalyzed cyclization and diversified by post-tailoring *** bifunctional terpene synthase(BFTS)associated P450 enzymes have shown significant catalytic potentials through the development of various new terpenoids with different biological *** study discovered the BFTS and its related gene cluster from the plant endophytic fungus Didymosphaeria variabile *** expression of the BFTS in Saccharomyces cerevisiae resulted in the characterization of a major product diterpene variediene(1),along with two new minor products neovariediene and *** heterologous expression of the BFTS and one cytochrome P450 enzyme VndE(CYP6138B1)in Aspergillus oryzae NSAR1 led to the identification of seven norditerpenoids(19 carbons)with a structurally unique 5/5 bicyclic ring ***,in vivo experiments suggested that the cyclized terpene variediene(1)was modified by VndE along with the endogenous enzymes from the host cell *** through serial chemical conversions,followed by multi-site hydroxylation via *** endogenous *** work revealed that the two-enzymes biosynthetic system and host cell machinery could produce structurally unique terpenoids.

关键词： Bifunctional terpene synthases Norditerpenoids Cytochrome P450 Aspergillus oryzae Heterologous expression

Transcatheter arterial embolization-salvaged ALPPS, a novel ALPPS procedure especially for patients with hepatocellular carcinoma and severe fibrosis/cirrhosis

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Hepatobiliary Surgery and Nutrition 2022年第4期11卷 504-514,I0001-I0003页

作者： Yuanfei Peng Zheng Wang xudong qu Feiyu Chen Huichuan Sun Xiaoying Wang Zhenbing Ding Min Tang Lei Yu Xinrong Yang Qiang Gao Zhaoyou Tang Wan Yee Lau Jia Fan Jian Zhou Department of Liver Surgery and Transplantation Liver Cancer InstituteZhongshan HospitalFudan UniversityKey Laboratory of Carcinogenesis and Cancer InvasionMinistry of EducationFudan UniversityShanghaiChina Department of Interventional Radiology Zhongshan HospitalFudan UniversityShanghaiChina Department of Radiology Zhongshan HospitalFudan UniversityShanghaiChina Faculty of Medicine The Chinese University of Hong KongPrince of Wales HospitalShatinHong KongChina Institute of Biomedical Sciences Fudan UniversityShanghaiChina State Key Laboratory of Genetic Engineering and Collaborative Innovation Center for Genetics and Development School of Life SciencesFudan UniversityShanghaiChina Shanghai Key Laboratory of Organ Transplantation ShanghaiChina

Background:The associating liver partition and portal vein ligation for staged hepatectomy(ALPPS)for hepatocellular carcinoma(HCC)with fibrosis/cirrhosis is often associated with limited growth of future liver remnant(FLR).We introduced a new procedure named transcatheter arterial embolization-salvaged ALPPS(TAE-salvaged ALPPS)which was shown to be especially suitable for HCC patients with cirrhosis or fibrosis who failed adequately to respond to conventional *** short-term efficacy and safety for the TAE-salvaged ALPPS on patients with HCC and fibrosis/cirrhosis were ***:Consecutive HCC patients who underwent TAE-salvaged ALPPS in our hospital between November 2016 and June 2020 were retrospectively *** new ALPPS procedure included conventional ALPPS stage-1 using associating liver partition and portal vein *** FLR failed to reach sufficient hypertrophy,TAE was carried out 2 weeks later followed by liver resection 3 weeks after ALPPS ***:Nine of 10 patients had a single tumor(median diameter 14.0 cm,range,5.2-17 cm).The remaining patient had multiple tumors(diameter of one tumor 14.0 cm,and two satellite foci 2.0 and 3.0 cm).R0 resection was achieved in all patients(100%)after a median of 21 *** patients had cirrhosis,1 had METAVIR grade-3 fibrosis,and 3 had METAVIR grade-2 *** median increase in FLR volume after TAE-salvaged ALPPS was 69.7%(34.4-143.9%).The absolute and relative kinetic growth rates(KGRs)were 9.9(7.1-17.3)mL/day and 3.4%(1.9-7.2%)/day,*** median absolute KGRs were 15.7,2.6,and 19.5 mL/day in the first,second,and third postoperative weeks after ALPPS stage-1,*** rapid increase in KGR on the third week was induced by *** overall postoperative morbidity rates were 50,0%(5/10),20.0%(2/10)and 70.0%(7/10)after ALPPS stage-1,TAE and ALPPS stage-2,*** 90-day mortality rate was 10.0%(1/10).The median overall survival was 40 ***:The new TA

关键词： Associating liver partition and portal vein ligation for staged hepatectomy(ALPPS) transcatheter arterial embolization(TAE) hepatocellular carcinoma(HCC) cirrhosis fibrosis

用于选择性优产雄烯二酮的工业小金色分枝杆菌的基因改造

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武汉大学学报（理学版） 2017年第1期63卷 51-56页

作者：余舒琪瞿旭东武汉大学药学院湖北武汉430072

工业上用于生产4-雄烯-4-醇-3,17-二酮(4-雄烯二酮,4-AD)的小金色分枝杆菌(Mycobacterium neoaurum)在降解植物甾醇侧链的过程中同时产生部分1,4-雄烯-4-醇-3,17-二酮(1,4-雄烯二酮,ADD),而3-甾酮-Δ1-脱氢酶KstD催化了4-AD到ADD的转化.克隆该工业菌株的kstD基因测序后发现,其与报道的标准分枝杆菌基因组中的kstD基因相比有3个氨基酸的突变(A6E,S138L,A193V).这些突变使得KstD的酶活受到影响,一定程度上阻碍4-AD向ADD的转变.为了实现从植物甾醇降解中选择性地得到4-AD或ADD,通过敲除kstD基因,阻断4-AD到ADD的转化,获得一株只产4-AD的突变菌株,代谢产物中4-AD达79.3%.通过修复kstD酶的氨基酸位点,使之恢复到文献报道的正常序列,并在敲除kstD基因菌株中,将修复后的kstD基因安置在强启动子pG13下,重新导回到突变菌株中进行回补强化表达,获得一株可以大量产生ADD的菌株.野生型分枝杆菌转化植物甾醇产生的产物中,ADD仅占27.0%,经回补强化表达后的基因工程菌株转化植物甾醇产生的产物中ADD占63.0%.结果表明,通过敲除或者在突变体菌株中过表达正常的kstD基因可以使该工业菌株选择性地优产4-AD或ADD.

关键词：甾醇降解 1,4-雄烯二酮 4-雄烯二酮 3-甾酮-Δ1-脱氢酶基因修复

微生物降解植物甾醇制备4-雄烯-4-醇-3,17-二酮的无油工艺

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武汉大学学报（理学版） 2017年第1期63卷 57-62页

作者：张亚楠瞿旭东武汉大学药学院湖北武汉430072

研究了一种利用工业用菌株野生小金色分枝杆菌(Mycobacterium neoaurum)催化降解甾醇制备4-雄烯-4-醇-3,17-二酮(4-雄烯二酮,4-AD)的无油工艺.从静态细胞转化法和发酵法中选择发酵法,研究了甾醇投料量、助溶剂、分散剂、金属离子、发酵... 详细信息

研究了一种利用工业用菌株野生小金色分枝杆菌(Mycobacterium neoaurum)催化降解甾醇制备4-雄烯-4-醇-3,17-二酮(4-雄烯二酮,4-AD)的无油工艺.从静态细胞转化法和发酵法中选择发酵法,研究了甾醇投料量、助溶剂、分散剂、金属离子、发酵时间等条件对菌株生长状态、发酵底物转化率和4-AD产量的影响,对助溶剂、分散剂、底物的用量和接种量4个较重要的因素进行3个水平的正交实验.最佳工艺为:采用发酵法,甾醇投料量30g/L、助溶剂羟丙基!-环糊精40g/L、分散剂吐温-80 5g/L、接种量20%、亚铁离子0.4g/L、发酵时间120h;4-AD的产量最高达到14.3g/L,转化率达到76%.

关键词：微生物降解植物甾醇 4-雄烯二酮无油工艺

理性设计提高乙醇脱氢酶LbADH的催化活性

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武汉大学学报（理学版） 2017年第1期63卷 45-50页

作者：王加丽杨怡瞿旭东武汉大学药学院湖北武汉430072

来源于革兰氏阳性短乳杆菌Lactobacillus brevis ATCC 367的乙醇脱氢酶LbADH是催化手性醇的模式酶,以四聚体的形式发挥催化活性.本研究通过计算机分子模拟进行理性设计,确定并设计LbADH中与单体间疏水相互作用及盐桥键形成相关的关键位... 详细信息

来源于革兰氏阳性短乳杆菌Lactobacillus brevis ATCC 367的乙醇脱氢酶LbADH是催化手性醇的模式酶,以四聚体的形式发挥催化活性.本研究通过计算机分子模拟进行理性设计,确定并设计LbADH中与单体间疏水相互作用及盐桥键形成相关的关键位点,通过定点突变在原始序列中引入相应的氨基酸,以改变单体间的结合能力,定向调节其催化活性和热稳定性.研究获得一个疏水突变体LbADH F146L,其在37℃下的酶活力为野生酶的8.5倍,在50℃下的酶活力为野生酶的5.6倍;其动力学参数Kcat/Km(1.99×106 L·mol-1·s-1)为野生酶(1.40×104 L·mol-1·s-1)的142倍,表明其催化效率明显提高.该研究结果表明,通过将LbADH中146位的苯丙氨酸(F146)突变为亮氨酸(L)增大疏水作用可以提高其催化活性.

关键词：乙醇脱氢酶分子模拟酶活力动力学疏水作用理性设计