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稀有金属材料与工程 2006年第A03期35卷 389-391页

作者： Xiaowen xu Xiaohao wang fei Tang Zhaoying Zhou State Key Laboratory of Precision Measurement Technology and Instruments Tsinghua University Beijing 100084 China

An amperometric biosensor based on acetylcholinesterase (ACHE) is assembled by simple adsorption of the AChE on 7,7,8,8-tetracyanoquinodimethane (TCNQ) modified screen-printed *** biosensor is used to detect the inhibitory effect of organophosphorus pesticides on AChE *** detection of organophosphorus pesticides is done with acetylthiocboline chloride (ATCh) as *** order to obtain the optimized response to substrate,the influencing factors of the biosensor are investigated,including temperature,pH,incubation time,substrate concentration and AChE concentration. The measurements were performed after inhibition by immersing the enzyme electrode into the parathion methyl solution. Under the optimized conditions,that is,500 U/ml AChE concentration,pH 7.2,10 min incubation time,2 mmol/L substrate concentration and temperature of 37℃,from 5×10^(-8) mol/L to 5×10^(-5) mol/L is close to linear (regression equation: y(%)=124.055+15.7991gx,R^2=0.99644),which corresponds to 8.7%～56.1% AChE *** the optimized conditions, the lowest detectable amount of parathion methyl is 13×10^(-9).

关键词： biosensor AChE screen-printed electrodes influencing factors organophosphorus pesticides

Microdosimetric Evaluation on the Metallic Nanoparticle-Mediated Dose Enhancement in Radiotherapeutic Proton Irradiation

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Chinese Physics Letters 2018年第6期35卷 106-110页

作者： Ai-Hui Feng Xiang Li xu-fei wang Xiao-Wa wang Institute of Modern Physics Fudan University Shanghai 200433 Laboratory of Nuclear Physics and Ion-beam Application Fudan University Shanghai 200433 Shanghai Proton and Heavy Ion Center Shanghai 201315

Monte Carlo simulations are performed on the dosimetric effect of metallic nanoparticles in a clinical proton *** an in-water hitting model of a single nanoparticle,the secondar.y electrons dose,deposited around the particle surface,is calculated for the proton irradiations in a typical spread-out Bragg *** dose enhancement,as the ratio of electron doses from the target particle and background water,is evaluated for the dependence on the depth of hitting,particle size,elements,coating material and *** results indicate a significant dose enhancement on the particle surface within-200 nm,but a fast decay in further *** dose enhancement presents a consistency along the spread-out Bragg peak,a positive dependence on both the particle size and electron density,but a strong attenuation by surface *** cluster may increase the incdividual dose enhajncement by electron crossfire,but is only noticeable in a compact *** dose enhancement potentiates a radiosensitization use of metallic nanoparticles in clinical proton therapy,but challenqging meanwhile with the narrow ranqge of enhancement effect.

关键词： Microdosimetric Evaluation on the Metallic Nanoparticle-Mediated Dose Enhancement in Radiotherapeutic Proton Irradiation

Large scale production of carbon nanotube arrays on the sphere surface from liquefied petroleum gas at low cost

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Chinese Science Bulletin 2007年第21期52卷 2896-2902页

作者： ZHANG Qiang HUANG JiaQi WEI fei xu GuangHui wang Yao QIAN WeiZhong wang DeZheng Beijing Key Laboratory of Green Chemical Reaction Engineering and Technology Department of Chemical Engineering TsinghuaUniversity Beijing 100084 China

Liquefied petroleum gas (LPG), a cheap industrial material, is used as carbon source to produce carbon nanotube (CNT) arrays on ceramic spherical surface on a large scale in the floating catalyst process. The ceramic spheres provide huge surface area and good mobility, leading to the mass production of CNT arrays continuously. The arrays obtained from the surface are of good alignment, and the purity is as high as 97.5%. With the decrease of the growth temperature, CNTs in the array form with small-diameter of about 13 nm can be obtained. Therefore, with the industrial fuel as carbon source and the ceramic sphere as substrate, CNT arrays can easily be produced on large scale at low cost.

关键词：液化气生产量成本碳纳米管阵列

Three-dimensional regulation of transcription

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Protein & Cell 2015年第4期6卷 241-253页

作者： Jun Cao Zhengyu Luo Qingyu Cheng Qianlan xu Yan Zhang fei wang Yan Wu Xiaoyuan Song CAS Key Laboratory of Brain Function and Disease and School of Life Sciences University of Science and Technology ofChina Hefei 230027 China

Cells can adapt to environment and development by reconstructing their transcriptional networks to regulate diverse cellular processes without altering the underly- ing DNA sequences. These alterations, namely epige- netic changes, occur during cell division, differentiation and cell death. Numerous evidences demonstrate that epigenetic changes are governed by various types of determinants, including DNA methylation patterns, his- tone posttranslational modification signatures, histone variants, chromatin remodeling, and recently discovered chromosome conformation characteristics and non- coding RNAs （ncRNAs）. Here, we highlight recent efforts on how the two latter epigenetic factors participate in the sophisticated transcriptional process and describe emerging techniques which permit us to uncover and gain insights into the fascinating genomic regulation.

关键词： transcriptional regulation long non-coding RNAs three-dimensional chromosome interactions,epigenetic changes

基于理想值和容忍偏差值的多目标交互式决策方法

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上海交通大学学报 1998年第3期32卷 35-39页

作者：徐飞王浣尘上海交通大学系统工程研究所

基于理想值和容忍偏差值的概念，提出了一种实用有效的交互式算法．该算法在交互过程中充分考虑了决策人的交互要求，灵活性强．而且所构造的辅助问题在锥有效解意义下仍适用，并可确保所得解的锥有效性．

关键词：多目标决策交互式理想值容忍偏差值

XAF1 as a prognostic biomarker and therapeutic target in squamous cell lung cancer

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Chinese Medical Journal 2011年第20期124卷 3238-3243页

作者： CHEN Yong-bing SHU Jian YANG Wen-tao SHI Li GUO xu-feng wang fei-ge QIAN Yong-yue Department of Cardiothoracic Surgery Second Affiliated HospitalSoochow University Suzhou Jiangsu 215004 China

Background X-linked inhibitor of apoptosis （XlAP）-associated factor 1 （XAF1） is a new tumor suppressor. Low expression of XAF1 is associated with poor prognosis of human cancers. However, the effect of XAF1 on lung cancer remains unknown. In this study, we investigated the expression of XAF1 and its role in squamous cell lung cancer. Methods Cancer tissues, cancer adjacent tissues and normal lung tissues were collected from 51 cases of squamous cell lung cancer. The expression of XAF1 mRNA was determined by reverse transcription-polymerase chain reaction （RT-PCR）. The expression of XAF1 protein was determined by Western blotting and immunohistochemical staining. Ad5/F35-XAF1 virus was generated. Cell proliferation and apoptosis were measured by 3-（4, 5-dimethylthiazol-2-yl）-2, 5-diphenyltetrazolium bromide （MTT） method and flow cytometry （FACS）, respectively. Results The levels of XAF1 protein and mRNA in cancer tissues were significantly lower than those in cancer adjacent and normal lung tissues （P 〈0.05）. The low expression of XAF1 was associated with tumor grade, disease stage, differentiation status and lymph node metastasis in squamous cell lung cancer patients. The restoration of XAF1 expression mediated by Ad5/F35-XAF1 virus significantly inhibited cell proliferation and induced apoptosis in a dose- and time-dependent manner. Conclusion XAF1 is a valuable prognostic marker in squamous cell lung cancer and may be a potential candidate gene for lung cancer therapy.

关键词： XAF1 human lung cancer cell lines cell proliferation apoptosis prognosis, biomarker

Effects of topiramate and carbamazepine on thyroid hormone level in adults with epilepsy

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Neural Regeneration Research 2006年第8期1卷 706-709页

作者： Liang Yu, Yulan Huang, Hongbin Sun, Jie Liu, fei xu, Xiaoping wang Sichuan Academy of Medical Sciences Department of Neurology, Sichuan Provincial People’s Hospital, Chengdu 610072, Sichuan Province, China Sichuan Academy of Medical Sciences Department of Neurology Sichuan Provincial People's Hospital Chengdu 610072 Sichuan Province China

BACKGROUND: It has been demonstrated that traditional antiepileptics, such as phenytoin, carbamazepine (CBZ), phenobarbital, etc., can result in the decrease of thyroid hormone of epileptic patients. However, there is still no sufficient evidence for the studies about the effect of new-type antiepileptics, such as topiramate (TPM), on thyroid hormones. OBJECTIVE: To observe the effects of TPM and CBZ on the level of thyroid hormones in serum of adults with epilepsy. DESIGN: A comparative observation. SETTING: Department of Neurology, Sichuan Provincial People's Hospital. PARTICIPANTS: Totally 100 outpatients or inpatients newly diagnosed to have epilepsy were selected from the Department of Neurology, Sichuan Provincial People's Hospital from July 2003 to August 2005, including 60 males and 40 females, aged 18-70 years. All the patients were accorded with the standard for the classification of epilepsy set by International League Against Epilepsy (ILAE) in 1981; Had been Informed and agreed with the detection; Had no history of thyroid gland disease; Had not taken any drugs could affect the thyroid function. Meanwhile, 40 adult healthy examinees were selected from our hospital as the control group, including 24 males and 16 females, aged 18-65 years. METHODS: ① The 100 epileptic patients were randomly divided into TPM group (n =50) and CBZ group (n =50), and they were treated with TPM (Xian-Janssen Pharmaceutical, Ltd.; Batch number: 03AS032, Norm: 25 mg/tablet) and CBZ (Shanghai Sunve Pharmaceutical Co., Ltd.; Batch number: 030201, Norm: 100 mg/tablet) respectively. The initial dosage of TPM was 25 mg per day, increased by 25 mg every week, the objective dosage of 100-200 mg per day was maintained when the symptoms were satisfactorily controlled. The dosage of CBZ was 6-8 mg/kg per day. All the patients were administrated for 1 year. ② The serum levels of total thyroxine (TT4), free thyroxine (FT4), total triiodothyronine (TT3), free triiodothyronine (FT3) and thyr

关键词： CBZ Effects of topiramate and carbamazepine on thyroid hormone level in adults with epilepsy

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非编码RNA药物的研究进展

生命科学 2018年第2期30卷 213-221页

作者：许从飞王均华南理工大学医学院生命科学研究院广州510006

非编码RNA(non-coding RNA,ncRNA)是指从DNA转录而来,不翻译成蛋白质的RNA分子。以前人们认为非编码RNA仅仅是基因表达的副产物,随着研究的深入,人们发现非编码RNA参与到生命活动的各个方面,尤其是多种疾病的发生过程。多种非编码RNA被... 详细信息

非编码RNA(non-coding RNA,ncRNA)是指从DNA转录而来,不翻译成蛋白质的RNA分子。以前人们认为非编码RNA仅仅是基因表达的副产物,随着研究的深入,人们发现非编码RNA参与到生命活动的各个方面,尤其是多种疾病的发生过程。多种非编码RNA被设计成药物,不少已经通过临床试验用于疾病治疗。核酸药物递送系统的发展为非编码RNA递送提供了解决方案,大大加快了非编码RNA药物的研究进展。现对近年来非编码RNA药物及药物递送系统进行综述,希望能够激发更多非编码RNA药物研究的热情和动力。

关键词：非编码RNA 药物递送系统药物研发

Research on Driver’s Face Detection and Position Method Based on Image Processing

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Research on Driver’s Face Detection and Position Method Bas...

MULTIPLE REGRESSION BASED RISK COST FORECASTING FOR VIRTUAL ORGANIZATION

第24届中国控制与决策会议

作者： Jia Mingxing, xu Hengyuan, wang fei Academy of Information Science and Engineer, Northeastern University, Shen yang, Liao ning 110004

This paper is presented research on face detection for fatigue driving detection. Due to the complicated situation when driving, conventional methods that applied in detecting driver’s face take a large amount of time and are at low accuracy rate. For this reason, an ellipse with self-learning radius is taken to instead of 2d Gaussian Distribution Model, and some other promoted methods are also used in this paper such as Integral Projection, Barycenter Adjustment method, etc. All of these achieve the purpose of positioning face accurately and quickly in various complicated cases such as complex illumination, small angles of side face, etc.

关键词： Fatigue Driving Skin color Model Ellipse Replace Integral Projection, Barycenter Adjustment

来源： cnki会议评论

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MULTIPLE REGRESSION BASED RISK COST FORECASTING FOR VIRTUAL ...