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brain-wide N2cG compensation permits glycoprotein-deleted rabies virus to trace neural circuits across multiple synapses

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Journal of Innovative Optical Health Sciences 2023年第6期16卷 13-21页

作者： Nengsong Luo Zengpeng Han Jiaxin Kou Yuxiang Cai Xin Yang Jie Wang Kunzhang Lin fuqiang xu Wuhan National Laboratory for Optoelectronics Huazhong University of Science and TechnologyWuhan 430074P.R.China State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics Key Laboratory of Magnetic Resonance in Biological SystemsWuhan Center for Magnetic ResonanceInnovation Academy for Precision Measurement Science and TechnologyChinese Academy of SciencesWuhan430071P.R.China University of Chinese Academy of Sciences Beijing100049P.R.China Shenzhen Key Laboratory of Viral Vectors for biomedicine Key Laboratory of Quality Control Technology for Virus-Based TherapeuticsGuangdong Provincial Medical Products AdministrationNMPA Key Laboratory for Research and Evaluation of Viral Vector Technology in Cell and Gene Therapy Medicinal Productsthe Brain Cognition and Brain Disease Institute(BCBDI)Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research InstitutionsShenzhen518055P.R.China Department of Pathophysiology Key Lab of Neurological Disorder of Education MinistrySchool of Basic MedicineTongji Medical CollegeHuazhong University of Science and TechnologyWuhanP.R.China Shenzhen-Hong Kong Institute of brain Science-Shenzhen Fundamental Research Institutions Shenzhen518055P.R.China Center for Excellence in brain Science and Intelligence Technology Chinese Academy of SciencesShanghai200031P.R.China

Rabies-viruses-based retrograde tracers can spread across multiple synapses in a retrograde direction in the nervous system of rodents and primates,making them powerful tools for determining the structure and function of the complicated neural circuits of the ***,they have some limitations,such as posing high risks to human health and the inability to retrograde trans-synaptic label inputs from genetically-de¯ned starter ***,we established a new retrograde trans-multi-synaptic tracing method through brain-wide rabies virus glycoprotein(RVG)compensation,followed by glycoprotein-deleted rabies virus(RV-△G)infection in specific brain ***,in combination with the avian tumor virus receptor A(TVA)controlled by a cell-type-specific promoter,we found that EnvA-pseudotyped RV-△G can mediate e±cient retrograde trans-multi-synaptic transduction from cell-type-specific starter *** study provides new alternative methods for neuroscience researchers to analyze the input neural networks of rodents and nonhuman primates.

关键词： Rabies virus retrograde tracers trans-multi-synaptic tracing AAV-PHP.eb celltype-specific input neural networks

Achieving highly stable Sn-based anode by a stiff encapsulation heterostructure

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Science China Materials 2022年第3期65卷 695-703页

作者： Ruizhe Li Jijian xu Zhuoran Lv Wujie Dong fuqiang Huang State Key Laboratory of High Performance Ceramics and Superfine Mincrostructure Shanghai Institute of CeramicsChinese Academy of SciencesShanghai 200050China Center of Materials Science and Optoelectronics Engineering University of Chinese of Academy of SciencesBeijing 100049China State Key Laboratory of Rare Earth Materials Chemistry and Applications College of Chemistry and Molecular EngineeringPeking UniversityBeijing 100871China

Rationally designed heterostructures provide attractive prospects for energy storage electrodes by combining different active materials with distinct electrochemical ***,through a phase separation strategy,a heterostructure of SnO_(2) encapsulated by amorphous Nb_(2)O_(5) is spontaneously ***-type anode Nb_(2)O_(5) outer shell,playing as reaction containers and fast ionic pathways,physically inhibits the Sn atoms’migration and enhances the reaction ***,strong chemical interactions are found at the SnO_(2)/Nb_(2)O_(5) interfaces,which ensure the solid encapsulation of the SnO_(2) cores even after 500 *** used for lithium-ion batteries,this heterostructured anode exhibits high cycling stability with a capacity of 626 mAhg^(-1) after 1000 cycles at 2Ag^(-1)(85% capacity retention)and good rate performance with the capacity of 340 mAhg^(-1) at 8Ag^(-1).

关键词： heterostructure encapsulation SnO2/Nb2O5 interface chemical interaction atom migration

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中国城市隧道遮光棚现状统计分析及展望

隧道建设（中英文） 2023年第5期43卷 887-897页

作者：许富强杜志刚梅家林韩磊武汉理工大学交通运输与物流工程学院湖北武汉430070

为促进隧道遮光棚的发展,全面阐述中国城市隧道遮光棚的研究进展和建设现状。首先,通过文献调研梳理国内外隧道遮光棚的研究成果;其次,借助百度实景地图,调研中国291座城市隧道遮光棚的建设现状;然后,以理论研究和建设实践对比的方式,... 详细信息

为促进隧道遮光棚的发展,全面阐述中国城市隧道遮光棚的研究进展和建设现状。首先,通过文献调研梳理国内外隧道遮光棚的研究成果;其次,借助百度实景地图,调研中国291座城市隧道遮光棚的建设现状;然后,以理论研究和建设实践对比的方式,总结城市隧道遮光棚的应用范围、设置长度、减光构造、棚体色彩、洞顶形态等特点。实践与理论的偏差主要体现为:1)遮光棚的应用比例低(43.4%)、长度较短(43.8 m);2)遮光棚的不适眩光问题显著(57.3%),背景色彩普遍未采用暗色调(86.1%);3)针对遮光棚诱导色彩和诱导形态的研究相对滞后。最后,针对存在的问题,提出相应的展望。

关键词：城市隧道隧道入口区域遮光棚减光构造棚体色彩洞顶形态

Rabies Virus Pseudotyped with CVS-N2C Glycoprotein as a Powerful Tool for Retrograde Neuronal Network Tracing

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Neuroscience bulletin 2020年第3期36卷 202-216页

作者： xutao Zhu Kunzhang Lin Qing Liu Xinpei Yue Huijie Mi Xiaoping Huang Xiaobin He Ruiqi Wu Danhao Zheng Dong Wei Liangliang Jia Weilin Wang Anne Manyande Jie Wang Zhijian Zhang fuqiang xu Shenzhen Key Lab of Neuropsychiatric Modulation and Collaborative Innovation Center for brain Science Guangdong Provincial Key Laboratory of Brain Connectome and BehaviorCAS Center for Excellence in Brain Science and Intelligence TechnologyBrain Cognition and Brain Disease Institute(BCBDI)Shenzhen Institutes of Advanced TechnologyChinese Academy of SciencesShenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research InstitutionsShenzhen 518055China State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics Key Laboratory of Magnetic Resonance in Biological SystemsWuhan Center for Magnetic ResonanceWuhan Institute of Physics and MathematicsChinese Academy of SciencesWuhan 430071China Wuhan National Laboratory for Optoelectronics Huazhong University of Science and TechnologyWuhan 430071China College of Life Sciences Wuhan UniversityWuhan 430071China School of Human and Social Sciences University of West LondonLondonUK University of the Chinese Academy of Sciences Beijing 100049China

Efficient viral vectors for mapping and manipulating long-projection neuronal circuits are crucial in structural and functional studies of the brain. The SAD strain rabies virus with the glycoprotein gene deleted pseudotyped with the N2 C glycoprotein(SAD-RV(DG)-N2 C(G)) shows strong neuro-tropism in cell culture, but its in vivo efficiency for retrograde gene transduction and neuro-tropism have not been systematically *** compared these features in different mouse brain regions for SAD-RV-N2 C(G) and two other widely-used retrograde tracers, SAD-RV(DG)-b19(G) and r AAV2-retro. We found that SAD-RV(DG)-N2 C(G) enhanced the infection efficiency of long-projecting neurons by^10 times but with very similar neuro-tropism, compared with SAD-RV(DG)-b19(G). On the other hand, SAD-RV(DG)-N2 C(G) had an infection efficiency comparable with r AAV2-retro, but a more restricted diffusion range, and broader tropism to different types and regions of longprojecting neuronal populations. These results demonstrate that SAD-RV(DG)-N2 C(G) can serve as an effective retrograde vector for studying neuronal circuits.

关键词： Viral vector N2C glycoprotein Neuronal circuits Retrograde tracing

Retrograde tracer systems based on Pseudorabies virus

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Retrograde tracer systems based on Pseudorabies virus

Trans-synaptic tracing in the olfactory circuits with theneurotropic viruses

中国神经科学学会第十二届全国学术会议

作者： Jia Fan Miu Huan xu Xiaoqin Lv Pei xu fuqiang brain research center Wuhan Institute of Physics and Mathematics Chinese Academy of Sciences University of Chinese Academy of Sciences Center for Excellence in brain Science and Intelligence Technology Chinese Academy of Sciences

Objective Neural circuit is the foundation of the brain function. Tracers based on neurotropic viruses, such as pseudorabies virus, rabies virus, vesicular stomatitis virus, herpes simplex virus, have been developed for displaying the full morphology of a neuron and mapping the neural circuit. In these systems, the pseudorabies virus is a DNA virus, it can infect swine, mouse, rat and other animals. However, it cannot infect human. The tracer based on the bartha strain, when it enters a neuron, it can transport in the neuron and spread from a neuron to the connected neuron with retrograde pattern. The tool could be used to determine the input circuit. The virus can be modified to develop various retrograde tools to resolve different questions. Methods Two tracer systems based on pseudorabies virus could be developed, i) transmonosynaptic tracer（specific cell type and non-specific cell type）: the TK gene is a switch, which could be deleted to prepare TK-deleted pseudorabies virus. When the helper virus expressing TK gene was injected into the target region, the TK was expressed and it can induce the replication of TK-deleted pseudorabies virus in neuron and assemble new viruses, then these new viruses can trans-monosynapse. ii) transmultisynaptic tracer（specific cell type and non-specific cell type）: the cre and flp sitespecific recombinases are excellent tools, and many transgenic animal models have been developed, such as PV-Cre, Thy1-Cre. based on these advantages, the pseudorabies virus could be modified to determine the cell type specific neural circuit. Results and Conclusion The recombinant viruses and the wild type viruses are similar in viral growth, and they can label neuron and depict the neural circuit with retrograde pattern.

关键词： Retrograde Pseudorabies virus Trans-monosynapse Trans-multisynapse

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Trans-synaptic tracing in the olfactory circuits with theneu...

中国神经科学学会第十届全国学术会议

作者： Xiaobin He Zhijian Zhang Sen Jin fuqiang xu State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics Wuhan Institute of Physics and Mathematicsthe Chinese Academy of Sciences University of Chinese Academy of Sciences Wuhan National Laboratory for Optoelectronics College of Life Science and Technology Huazhong University of Science and Technology

Objective In the mammalian brain,all of the sensory processes are strictly regulated by the higher center of brain,such as attention,emotion,motivation,and experience et al,which are essential for individuals to guarantee survival,promote reproduction and permit higher functions like learning and *** the olfactory system,the top-down regulated processing of olfactory information in the olfactory bulb is believed to play a crucial role in regulation of olfactory-related *** further understand the circuitry mechanism of the top-down regulated olfactory processing,we applied trans-synaptic tracing with two different neurotrophic viruses to retrograde label the neural network that innervate the olfactory bulb at mono- or multipl- synaptic *** To specifically label the neurons that directly innervate the olfactory bulb and piriform cortex,we injected recombinant rabies viruses that expressing GFP and RFP respectively,into the olfactory bulb and the anterior piriform cortex *** addition,we injected a recombinant pseudorabeis viruses expressing EGFP（PRV152） into the olfactory bulb,and specifically labeled the neural network that innervate the olfactory bulb at multiple synaptic ***（1） In the retrograde tracing with rabies virus,the neurons that retrograde labeled from the olfactory bulb were observed distributing in several regions in the olfactory cortex,especially in the anterior part;The neurons that retrograde labeled from the anterior piriform cortex broadly distributed in hippocampus,entorhinal cortex,cortical region of amygdale,and hypothalamus.（2） In trans-multiple synaptic tracing with PRV152,the labeled neurons distributed broadly in various regions beyond olfactory *** addition,the dopaminergic neurons in the Pvn,Arc and the dorsal lateral hypothalamus,were observed co-labeled with *** Neurotrophic virus mediated trans-synaptic tracing could effectively extract the connecting information of the int

关键词： olfactory system neural circuit centrifugal projection olfactory bulb pseudorabeis virus rabies virus tracing

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页岩气储层孔隙压力测井预测新方法

石油学报 2017年第6期38卷 666-676页

作者：徐春露孙建孟董旭赖富强中国石油大学地球科学与技术学院山东青岛266580 重庆科技学院复杂油气田勘探开发重庆市重点实验室重庆401331

页岩气储层中常出现明显的异常高压现象,其孔隙压力会在短距离内大幅上升。若不能及时准确预测这些异常高压值,可能导致井涌、井喷等钻井事故,不仅会给钻井施工造成重大损失,还严重影响储层评价工作。然而,现有的方法却很难准确预测页... 详细信息

页岩气储层中常出现明显的异常高压现象,其孔隙压力会在短距离内大幅上升。若不能及时准确预测这些异常高压值,可能导致井涌、井喷等钻井事故,不仅会给钻井施工造成重大损失,还严重影响储层评价工作。然而,现有的方法却很难准确预测页岩气储层中的异常孔隙压力值。结合页岩气岩心实验数据,在充分考虑孔隙度、有机质含量和有效应力对声波速度影响的基础上,提出了一套基于声波速度模型的页岩气储层孔隙压力预测新方法。该方法通过使用不含气层段的纵波、横波速度关系对储层纵波速度进行含气性校正,消除了气体对异常高压现象在声波测井信息表征上的影响。此外,该方法还能预测因不平衡压实和生烃作用等多种机理引发的页岩气储层异常孔隙压力,弥补了现有传统方法只能预测由不平衡压实引起异常高压的不足。以昭通地区页岩地层S井为例进行储层孔隙压力预测,形成了基于声波速度模型的储层孔隙压力预测方法技术流程。

关键词：页岩气储层孔隙压力预测有机质含气性校正声波速度

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大跨度钢桁梁桥整体吊装施工吊点布置研究

钢结构 2015年第12期30卷 97-100页

作者：徐富强江克斌刘义解放军理工大学野战工程学院南京210007 解放军工程兵学院江苏徐州221004

在大跨度钢桁梁桥整体吊装施工中,为了使结构杆件整体内力和变形较小,需要对吊点的布置位置进行合理选择,基于应变能最小原理对大跨度钢桁梁桥整体吊装施工中吊点的布置位置进行研究。通过建立96m简支钢桁梁桥吊装模型,在各自吊点的备... 详细信息

在大跨度钢桁梁桥整体吊装施工中,为了使结构杆件整体内力和变形较小,需要对吊点的布置位置进行合理选择,基于应变能最小原理对大跨度钢桁梁桥整体吊装施工中吊点的布置位置进行研究。通过建立96m简支钢桁梁桥吊装模型,在各自吊点的备选节点区域内进行吊点变量组合,确立整体应变能最小的吊点组合方式即为最优吊点布置方式。针对实际吊装施工中的最不利荷载工况,对比分析最优吊点布置方式和其他两种吊点布置方式下的吊装结构受力状态。计算结果表明:采用应变能最小原理选择的吊点布置方式,吊装结构的受力状态更加合理。

关键词：钢桁梁桥整体吊装吊点布置应变能原理风荷载

Anterograde Monosynaptic Transneuronal Tracers Derived from Herpes Simplex Virus 1 strain H129

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Anterograde Monosynaptic Transneuronal Tracers Derived from ...