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经鼻高流量氧疗气道护理在高血压脑出血术后肺部感染中的应用

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临床医药实践 2024年第5期33卷 382-386页

作者：钟秀英李文慧曾玉鸿袁婷吴俊抚州市第一人民医院江西抚州344000

目的:探讨加温加湿经鼻高流量氧疗气道护理联合乙酰半胱氨酸雾化吸入在高血压脑出血术后肺部感染中的应用效果。方法:选择2022年10月—2023年5月收治的62例高血压脑出血术后非机械通气患者,随机分为对照组和观察组,每组31例。对照组采... 详细信息

目的:探讨加温加湿经鼻高流量氧疗气道护理联合乙酰半胱氨酸雾化吸入在高血压脑出血术后肺部感染中的应用效果。方法:选择2022年10月—2023年5月收治的62例高血压脑出血术后非机械通气患者,随机分为对照组和观察组,每组31例。对照组采用氧驱面罩雾化器气道湿化护理联合乙酰半胱氨酸雾化吸入治疗,观察组采用加温加湿经鼻高流量氧疗气道护理联合乙酰半胱氨酸雾化吸入治疗。比较两组血气指标、气道阻力(Raw)、痰液黏稠度、气道湿化评分与舒适度评分,统计两组不良反应发生情况以及总满意度。结果:干预后两组动脉血二氧化碳分压(PaCO_(2))、Raw低于干预前,且观察组低于对照组(P<0.05)。干预后两组动脉血氧分压(PaO_(2))及血氧饱和度(SaO_(2))高于干预前,且观察组高于对照组(P<0.05)。干预后观察组痰液黏稠度情况优于对照组(P<0.05)。观察组气道湿化评分、舒适度评分及并发症总发生率低于对照组(P<0.05)。观察组满意度高于对照组(P<0.05)。结论:乙酰半胱氨酸雾化吸入联合加温加湿经鼻高流量氧疗气道护理可明显改善高血压脑出血术后肺部感染患者血气指标,降低痰液黏稠度,患者舒适度更高,不良反应更少,满意度更高。

关键词：加温加湿经鼻高流量氧疗乙酰半胱氨酸雾化吸入高血压脑出血肺部感染血气指标痰液黏稠度气道湿化评分

KDELR2 promotes breast cancer proliferation via HDAC3-mediated cell cycle progression

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Cancer Communications 2021年第9期41卷 904-920页

作者： Haoran Wei Wenhao Ma Xiaofei Lu Haiying Liu Kashuai Lin Yinghui Wang Zijian Ye Linchong Sun Zhitong Huang Tingting Pan Zilong Zhou Eric Y.Cheng Huafeng Zhang Ping Gao xiuying zhong Hefei National Laboratory for Physical Sciences at Microscale the Chinese Academy of Sciences Key Laboratory of Innate Immunity and Chronic DiseaseSchool of Basic Medical SciencesDivision of Life Science and MedicineUniversity of Science and Technology of ChinaHefeiAnhui 230027P.R.China School of Medicine and Institutes for Life Sciences South China University of TechnologyGuangzhouGuangdong 510006P.R.China Division of Life Sciences and Medicine The First Affiliated Hospital of University of Science and Technology of ChinaUniversity of Science and Technology of ChinaHefeiAnhui 230027P.R.China College of Pharmacy University of North Texas Health Science CenterFortWorthTexas 76107USA

Background:Histone deacetylases(HDACs)engage in the regulation of various cellular processes by controlling global gene *** dysregulation of HDACs leads to carcinogenesis,making HDACs ideal targets for cancer ***,the use of HDAC inhibitors(HDACi)as single agents has been shown to have limited success in treating solid tumors in clinical *** study aimed to identify a novel downstream effector of HDACs to provide a potential target for combination ***:Transcriptome sequencing and bioinformatics analysis were performed to screen for genes responsive toHDACi in breast cancer *** effects of HDACi on cell viability were detected using the MTT *** mRNA and protein levels of genes were determined by quantitative reverse transcription-PCR(qRT-PCR)andWestern *** cycle distribution and apoptosis were analyzed by flow *** binding of CREB1(cAMP-response element binding protein 1)to the promoter of the KDELR(The KDEL(Lys-Asp-Glu-Leu)receptor)gene was validated by the ChIP(chromatin immunoprecipitation assay).The association between KDELR2 and protein of centriole 5(POC5)was detected by immunoprecipitation.A breast cancer-bearing mouse model was employed to analyze the effect of the HDAC3-KDELR2 axis on tumor ***:KDELR2 was identified as a novel target of HDAC3,and its aberrant expression indicated the poor prognosis of breast cancer *** found a strong correlation between the protein expression patterns of HADC3 and KDELR2 in tumor tissues from breast cancer *** results of the ChIP assay and qRT-PCR analysis validated that HDAC3 transactivated KDELR2 via *** HDAC3-KDELR2 axis accelerated the cell cycle progression of cancer cells by protecting the centrosomal protein POC5 from proteasomal ***,the HDAC3-KDELR2 axis promoted breast cancer cell proliferation and tumorigenesis in vitro and in ***:Our results uncovered a previously unappreciated fun

关键词： breast cancer histone deacetylase inhibitor KDELR2 HADC3 CREB1 protein of centriole 5

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彰显数学意识:幼儿数学教学的诉求

学前教育研究 2007年第3期 30-33页

作者：陈婷仲秀英西南大学数学与统计学院重庆400715 重庆师范大学经济与管理学院重庆400047

幼儿园数学教育对幼儿数学意识的生成起着关键的作用。幼儿数学意识就是幼儿能直观感知、领会他们生活中的数、形及其关系,并能主动、自觉地感受和运用数、量、形、数学符号、简单的数学方法的态度和意识。镶嵌式数学教学是彰显幼儿数... 详细信息

幼儿园数学教育对幼儿数学意识的生成起着关键的作用。幼儿数学意识就是幼儿能直观感知、领会他们生活中的数、形及其关系,并能主动、自觉地感受和运用数、量、形、数学符号、简单的数学方法的态度和意识。镶嵌式数学教学是彰显幼儿数学意识教学的有效途径;问题、操作、挑战和兴趣是彰显幼儿数学意识教学的有效条件;宽容幼儿数学学习中的“不正确”行为是彰显幼儿数学意识教学的环境保障。

关键词：幼儿数学意识数学教学

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管窥课程改革的障碍：教学价值观冲突的视角

辽宁教育研究 2007年第9期 74-77页

作者：周先进仲秀英赣南师范学院江西赣州341000 重庆师范大学重庆400047

教学价值观的冲突主要体现在教学本位价值的信念冲突、课程价值的信念冲突、教学价值获得方式的信念冲突和教学价值评价的信念冲突等方面。基于教学价值观冲突的视角,新课程改革的主要障碍表现:知识、技能,过程和方法,抑或情感、态度和... 详细信息

教学价值观的冲突主要体现在教学本位价值的信念冲突、课程价值的信念冲突、教学价值获得方式的信念冲突和教学价值评价的信念冲突等方面。基于教学价值观冲突的视角,新课程改革的主要障碍表现:知识、技能,过程和方法,抑或情感、态度和价值观,教学基本价值冲突的障碍;课程是知识,还是经验,课程价值观冲突的障碍;独白式教学,还是对话式教学,教学价值获得方式冲突的障碍;量化评价,还是质性评价,教学价值评价方式冲突的障碍。

关键词：课程改革教学价值观障碍冲突

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新型激光模型实现SPP增益补偿的数学分析

实验室研究与探索 2017年第9期36卷 25-28页

作者：褚丽娜仲秀英重庆科创职业学院重庆402100 重庆师范大学数学科学学院重庆400047

设计了有机染料分子的球形波导结构,采用最内层金属金(Au)、外层Si介质的多层球形结构,在环形介质中植入有机染料分子作为增益耦合介质。理论上分析了模型的的受激辐射磁场能量、辐射谱以及有机染料分子增益效果。特性分析结果表明:光... 详细信息

设计了有机染料分子的球形波导结构,采用最内层金属金(Au)、外层Si介质的多层球形结构,在环形介质中植入有机染料分子作为增益耦合介质。理论上分析了模型的的受激辐射磁场能量、辐射谱以及有机染料分子增益效果。特性分析结果表明:光波在有机染料分子增益区能够与内层金属实现耦合,发生了多层电场局域化现象,增益对金属-介质间的表面等离子有一定的补偿效果,从增益的高折射区与低折射率区间发生了全反射,实行了表面等离子传播的增益补偿效果。能够通过调节波导参数实现损耗-补偿控制,高折射区能够实现SPP补偿,有机染料分子距离核区越近补偿效果更好,损耗更小。

关键词：表面等离子激光器损耗-补偿有机染料分子系统不稳定性

The Application of Nicotiana benthamiana as a Transient Expression Host to Clone the Coding Sequences of Plant Genes

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American Journal of Molecular Biology 2024年第2期14卷 54-65页

作者： Jianzhong Huang Peng Jia Xiaoju zhong xiuying Guan Hongbin Zhang Honglei Ruan Key Laboratory of Chronic Diseases Fuzhou Medical University Fuzhou China

Coding sequences (CDS) are commonly used for transient gene expression, in yeast two-hybrid screening, to verify protein interactions and in prokaryotic gene expression studies. CDS are most commonly obtained using complementary DNA (cDNA) derived from messenger RNA (mRNA) extracted from plant tissues and generated by reverse transcription. However, some CDS are difficult to acquire through this process as they are expressed at extremely low levels or have specific spatial and/or temporal expression patterns in vivo. These challenges require the development of alternative CDS cloning technologies. In this study, we found that the genomic intron-containing gene coding sequences (gDNA) from Arabidopsis thaliana, Oryza sativa, Brassica napus, and Glycine max can be correctly transcribed and spliced into mRNA in Nicotiana benthamiana. In contrast, gDNAs from Triticum aestivum and Sorghum bicolor did not function correctly. In transient expression experiments, the target DNA sequence is driven by a constitutive promoter. Theoretically, a sufficient amount of mRNA can be extracted from the N. benthamiana leaves, making it conducive to the cloning of CDS target genes. Our data demonstrate that N. benthamiana can be used as an effective host for the cloning CDS of plant genes.

关键词： Coding Sequence Genomic Sequence Nicotiana benthamiana Plant Genes

Structural and Functional Insights into an Arabidopsis NBS-LRR Receptor in Nicotiana benthamiana

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American Journal of Molecular Biology 2024年第2期14卷 84-96页

作者： Jianzhong Huang xiuying Guan Xiaoju zhong Peng Jia Hongbin Zhang Honglei Ruan Key Laboratory of Chronic Diseases Fuzhou Medical University Fuzhou China

Nucleotide-binding site leucine-rich repeat receptors (NBS-LRR/NLRs) are crucial intracellular immune proteins in plants. Previous article reported a novel NLR protein SUT1 (SUPPRESSORS OF TOPP4-1, 1), which is involved in autoimmunity initiated by type one protein phosphatase 4 mutation (topp4-1) in Arabidopsis, however, its role in planta is still unclear. This study employed Nicotiana benthamiana, a model platform, to conduct an overall structural and functional analysis of SUT1 protein. The transient expression results revealed that SUT1 is a typical CNL (CC-NBS-LRR) receptor, both fluorescence data and biochemical results showed the protein is mainly anchored on the plasma membrane due to its N-terminal acylation site. Further truncation experiments announced that its CC (coiled-coil) domain possessed cell-death-inducing activity. The outcomes of point mutations analysis revealed that not only the CC domain, but also the full-length SUT1 protein, whose function and subcellular localization are influenced by highly conserved hydrophobic residues. These research outcomes provided favorable clues for elucidating the activation mechanism of SUT1.

关键词： CC-NBS-LRR Hypersensitive Response Nicotiana benthamiana Plasma Membrane Localization