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基于单细胞转录组测序的鹿茸生长中心细胞异质性研究

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黑龙江畜牧兽医 2024年第19期 108-112,123-127页

作者：丁宁张然然范广轩邢秀梅刘欣东北林业大学野生动物与自然保护地学院哈尔滨150040 中国农业科学院特产研究所特种经济动物分子生物学重点实验室长春130112

为了从单细胞水平探究梅花鹿鹿茸生长中心中细胞类型和基因表达的差异,即细胞异质性,试验采用单细胞转录组测序的方法,对生长75 d的梅花鹿鹿茸生长中心进行单细胞转录组分析,鉴定细胞类型,并对不同类型细胞进行基因表达模式、基因功能... 详细信息

为了从单细胞水平探究梅花鹿鹿茸生长中心中细胞类型和基因表达的差异,即细胞异质性,试验采用单细胞转录组测序的方法,对生长75 d的梅花鹿鹿茸生长中心进行单细胞转录组分析,鉴定细胞类型,并对不同类型细胞进行基因表达模式、基因功能富集分析。结果表明:鹿茸生长中心包含了内皮细胞、周细胞、间充质细胞、免疫细胞、软骨细胞和成骨细胞等6种细胞类型,其中内皮细胞特异性表达PECAM1和VWF基因;周细胞特异性表达ABCC9和KCNJ8基因;间充质细胞高表达PRRX1和POSTN基因;免疫细胞特异性表达PTPRC和LCP1基因;成骨细胞特异性表达PHEX基因;软骨细胞特异性表达IGF1R基因。说明鹿茸的生长中心有着丰富的细胞类型,鹿茸的生长发育需要这些细胞的协调作用。

关键词：梅花鹿鹿茸单细胞转录组测序骨发育细胞异质性

吉林梅花鹿血液生理生化指标及与生长性能的相关性

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黑龙江畜牧兽医 2024年第7期 119-124页

作者：周宏达高鹤轩王天骄杨苏坤邢秀梅中国农业科学院特产研究所特种经济动物分子生物学重点实验室长春130112

为了探究梅花鹿的血液生理生化指标及其与梅花鹿生长性能的相关性,试验测定了68头成年吉林梅花鹿(公鹿36头、母鹿32头)的生长性能,并采集血液样本测定生理生化指标,再应用双变量Pearson检验进行生长性能与血液生理生化指标的相关性分析... 详细信息

为了探究梅花鹿的血液生理生化指标及其与梅花鹿生长性能的相关性,试验测定了68头成年吉林梅花鹿(公鹿36头、母鹿32头)的生长性能,并采集血液样本测定生理生化指标,再应用双变量Pearson检验进行生长性能与血液生理生化指标的相关性分析。结果表明:母鹿的血红蛋白含量、白球比、钙含量极显著高于公鹿(P<0.01);母鹿的血小板数,碱性磷酸酶、淀粉酶活性,磷、总胆汁酸含量极显著低于公鹿(P<0.01)。公鹿的血小板数、总蛋白含量与体重、体高极显著正相关(P<0.01);母鹿的血小板数与体重显著极正相关(P<0.01);母鹿的总蛋白含量与胸深极显著正相关(P<0.01);公鹿的白细胞数、淋巴细胞数、中性粒细胞数与茸重极显著正相关(P<0.01)。说明成年梅花鹿公鹿与母鹿的血液生理生化指标存在一定的差异,血小板数和总蛋白含量与体重、体尺正相关,白细胞数与茸重正相关。

关键词：吉林梅花鹿血液生理生化指标生长性能相关性

基于SNP位点的吉林梅花鹿分子系谱构建及群体遗传结构分析

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畜牧兽医学报 2024年第9期55卷 3925-3935页

作者：范广轩王天骄董依萌王洪亮丁宁王莘皓邢秀梅中国农业科学院特产研究所农业农村部特种经济动物遗传育种与繁殖重点实验室长春130112 东北林业大学野生动物与自然保护地学院哈尔滨150006 广东长隆集团有限公司珠海519000

旨在利用全基因重测序技术获得的SNP位点为吉林梅花鹿保种群构建分子系谱,并对其遗传结构进行分析。本研究在锯茸期采集保种群的吉林梅花鹿血液10 mL(n=425),其中成年公鹿132只、成年母鹿208只、仔鹿85只,提取DNA后进行全基因重测序。... 详细信息

旨在利用全基因重测序技术获得的SNP位点为吉林梅花鹿保种群构建分子系谱,并对其遗传结构进行分析。本研究在锯茸期采集保种群的吉林梅花鹿血液10 mL(n=425),其中成年公鹿132只、成年母鹿208只、仔鹿85只,提取DNA后进行全基因重测序。通过计算血源同源系数(IBD)分析吉林梅花鹿亲缘关系;基于观测杂合度(Ho)、期望杂合度(He)、多态信息含量(PIC)、系统发育树和群体分化指数(Fst)等对吉林梅花鹿保种群遗传结构进行分析。通过全基因重测序筛选出25548601个高质量SNPs,425个样本共组成了90100对亲缘关系,平均亲缘系数为0.4351,得出195对父子关系与143对母子关系,占到采样群体原始系谱记录的92%,检出原始系谱错误率为2.2%,纠正了原始记录错误8处,具有较高的准确性和可靠性;分析群体分化指数(Fst),东丰型吉林梅花鹿与伊通型距离较远;绘制系统发育树,将吉林梅花鹿保种群划分为17个家系;采用的SNP标记平均观测杂合度为0.456,平均期望杂合度为0.277,平均多态信息含量为0.627,吉林梅花鹿保种群平均近交系数为0.078,存在较弱的近交。本研究建立了吉林梅花鹿保种群的分子系谱并对其群体遗传结构进行了分析,这对于保护吉林梅花鹿遗传多样性和规划保种群后续选育计划至关重要。

关键词：吉林梅花鹿 SNP 分子系谱

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梅花鹿耳成纤维细胞体外培养及转染条件优化

吉林农业大学学报 2024年第2期46卷 315-323页

作者：高鹤轩荣敏刘汇涛唐丽昕周宏达邢秀梅中国农业科学院特产研究所特种经济动物分子生物学重点实验室长春130112

为开展目标蛋白质在梅花鹿耳成纤维细胞的功能研究提供理论和技术依据,培养梅花鹿耳成纤维细胞,优化脂质体转染梅花鹿耳成纤维细胞的条件,以获得最优质粒转染参数。采用组织块贴壁法和差速贴壁法分离梅花鹿耳成纤维细胞,观察细胞基本形... 详细信息

为开展目标蛋白质在梅花鹿耳成纤维细胞的功能研究提供理论和技术依据,培养梅花鹿耳成纤维细胞,优化脂质体转染梅花鹿耳成纤维细胞的条件,以获得最优质粒转染参数。采用组织块贴壁法和差速贴壁法分离梅花鹿耳成纤维细胞,观察细胞基本形态特征变化,免疫荧光法鉴定波形蛋白(Vimentin)的表达;以增强型绿色荧光蛋白(EGFP)基因为标记基因,利用LipofectamineTM 3000介导外源DNA转染梅花鹿耳成纤维细胞,探讨质粒DNA与脂质体比例(1.0∶1.0,1.0∶1.5,1.0∶2.0,1.0∶2.5,1.0∶3.0)、质粒用量(0.5,1.0,1.5,2.0,2.5μg)、细胞传代次数(第2~6代)、细胞初始接种密度(50%,60%,70%,80%,90%)、转染效果观测时间(12,24,36,48,60 h)对转染效率的影响。结果表明:组织块贴壁法培养的梅花鹿耳成纤维细胞在倒置荧光显微镜下可见长梭形细胞生长,细胞免疫荧光检测波形蛋白(Vimentin)的表达结果为阳性;筛选的最佳转染条件:当质粒DNA与脂质体比例为1.0∶2.0,质粒DNA用量为1.5μg,细胞传代次数为第3代,细胞初始接种密度为70%,转染后48 h进行观测时,即可获得较佳的转染效率。研究成功分离培养梅花鹿耳成纤维细胞,筛选出了脂质体转染梅花鹿耳成纤维细胞的最佳条件,可为梅花鹿基因工程的相关研究提供参考依据。

关键词：梅花鹿成纤维细胞细胞培养脂质体转染

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牛、羊和鹿科动物遗传多样性及起源研究进展

特产研究 2024年第4期46卷 150-155页

作者：杨苏坤陈旭唐丽昕董世武王洪亮邢秀梅中国农业科学院特产研究所农业农村部特种经济动物遗传育种与繁殖重点实验室吉林长春130112 东北林业大学野生动物与自然保护地学院黑龙江哈尔滨150006

牛、羊和鹿资源是人类的宝贵财富,不仅能够为人类提供丰富的肉奶等产品,而且在医药、科学研究等方面有着无可替代的价值,但这些遗传资源正在丢失并且部分品种已经濒危甚至灭绝。探究其遗传多样性和起源可全面的了解群体现状、遗传变异... 详细信息

牛、羊和鹿资源是人类的宝贵财富,不仅能够为人类提供丰富的肉奶等产品,而且在医药、科学研究等方面有着无可替代的价值,但这些遗传资源正在丢失并且部分品种已经濒危甚至灭绝。探究其遗传多样性和起源可全面的了解群体现状、遗传变异和分化以及明确祖先类型和迁徙路线,并提出科学的保种选育方案,为遗传资源保护提供科学依据。本文综述了牛、羊和鹿科动物的遗传多样性的研究方法以及父系、母系起源的研究进展,为保护种质资源和濒危物种以及遗传资源的开发利用提供参考依据。

关键词：遗传标记遗传多样性父系起源母系起源

Analysis of the Indicence and Survival of Female Breast Cancer Patients in Beijing Over a 20-Year Period

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Chinese Journal of Clinical Oncology 2006年第6期3卷 433-436页

作者： Qijun Wang Weixing Zhu xiumei xing Chenxu Qu Department of Epidemiology Beijing Institute for Cancer Research School of Clinical Oncology Peking University Beijing 100036 China

OBJECTIVE To provide evidence for breast cancer prevention and control through epidemiological analysis of the incidence, mortality and survival rate of female breast cancer patients in Beijing. METHODS The female registration data in the Beijing urban area from 1982 to 2001 were retrospectively reviewed. The incidence, mortality and survival rate of female breast cancer patients were analyzed using routine and life-table statistical methods. RESULTS During the period of 1982 to 2001, there was a trend of an average annual increase of female breast cancer incidence of 4.6% in urban Beijing, and of 4,9% in the world-population standardized incidence. The epidemiological features of urban Beijing female breast cancer showed：（1）The incidence distribution of different age groups from 25 to 80 years elevated with two peaks at ages of 45- and 70- years; （2）There was an elevation in each age group over the last 20 years; （3）The incidence rate at ages of 35 to 64 reached 95.3/10^5, causing breast cancer to become the number one cancer in females. The changes in the survival rate showed the following： the 5-year observed survival rate （OSR） increased from 62.0% in 1982～1983 to 68.7% in 1987-1988, and the relative-survival rate （RSR） increased from 6613% to 74.2%. The 10-year OSR and RSR in 1987～1988 were 60.3% and 65.1%, and at 15 years 57.5% and 61.3%, respectively. The mortality rate of breast cancer patients fluctuated from 8 to 10 per 105 population over the 20 years of study. CONCLUSION There is a trend of an annual increase in female breast cancer in Beijing. The 5-year survival is being improved gradually while the mortality remains stable. The results demonstrate that the principles of ＂early prevention, diagnosis and treatment＂ for breast cancer are effective in Beijing.

关键词： breast neoplasms inddence mortality survival rate,

Effects of dietary protein level on nutrients digestibility and reproductive performance of female mink(Neovison vison)during gestation

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Animal Nutrition 2015年第2期1卷 65-69页

作者： Qingkui Jiang Guangyu Li Tietao Zhang Haihua Zhang Xiuhua Gao xiumei xing Jiaping Zhao Fuhe Yang State Key Laboratory of Special Economic Animal Molecular Biology Institute of Special Economic Animal and Plant Science Chinese Academy of Agricultural Sciences Feed Research Institute Chinese Academy of Agricultural Sciences

The objective of this study was to determine whether nutrient digestibility and reproductive performance of pregnant mink(Neovison vison) were affected by different dietary protein levels. One hundred and twenty female mink were randomly assigned to four groups, receiving diets of fresh material with different protein levels. The dietary protein levels,expressed as percentage of dry matter(DM),were 32,36, 40 and 44% respectively. These values corresponded to average 320, 360, 400 and 440 g protein/kg DM, respectively. Results were as follows. All of crude protein digestibility, nitrogen(N) intake, N retention increased along with dietary protein level increasing. Low protein level(32%) significantly reduced the above indicators(P < 0.05). DM digestibility and ether extract digestibility were not affected by dietary protein level. Results of mated females, barren females, kids per litter, live born kids per mated female, birth survival rate, and birth weight showed that mink achieved optimal reproductive performance when dietary protein level was 36%. In conclusion, dietary protein was anticipated to significantly influence some nutrients' utilization. Adopting the appropriate dietary protein level allow better reproduction performance. The most preferable reproductive performance was achieved when diet contained 275.5 g digestible protein per kg DM for female mink in gestation.

关键词： Female mink Protein level Gestation Reproductive performance

Mitochondrial genomes of Tapes dorsatus and Cardita variegata:insights into Heteroconchia phylogeny

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Journal of Oceanology and Limnology 2024年第3期42卷 943-959页

作者： Xumin WANG Hua ZHANG Xindong TENG Wenhui SUN Zhikai xing Shuang WANG xiumei LIU Jiangyong QU Lijun WANG College of Life Science Yantai UniversityYantai 264005China Qingdao International Travel Healthcare Center Qingdao 266071China

Heteroconchia,a widespread and abundant aquatic invertebrate,is an important clade of bivalve *** relationship between the three branches of Heteroconchia,Palaeoheterodonta,Archiheterodonta,and Euheterodonta has become a main controversy in molecular studies of the relationships between *** the present study,we assembled the complete mitochondrial genomes of Tapes dorsatus(Veneridae)and Cardita variegata(Carditidae)using high-throughput *** is the first mitochondrial genome belonging to the family Carditidae to be *** used 12 protein coding genes(excluding atp8)from the complete mitochondrial genomes of 146 species to recover the internal relationships of *** results support the traditional view of early branching of Palaeoheterodonta and the recovery of the monophyly of Palaeoheterodonta,Anomalodesmata,*** analysis show that gene arrangement within Venerida was highly ***-calibrated phylogenetic studies based on a relaxed molecular clock model suggested that Veneridae originated approximately 337.62 million years ago(Ma)and split into two major clades,whereas Carditidae originated approximately 510.09 *** results provide evidence of the internal relationships of Heteroconchia.

关键词： Tapes dorsatus Cardita variegata mitochondrial genome phylogeny

Analysis on Growth and Development Laws of Different Mink Varieties

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Agricultural Biotechnology 2018年第5期7卷 122-123页

作者： Min RONG Jianfeng TU Zhiming ZHANG xiumei xing Institute of Special Wild Economic Animals and Plants CAAS State Key Laboratory of Special Economic Animal Molecular BiologyChangchun 130112China Dalian Mingwei Mink Limited Corporation Dalian 116100China

This study was conducted to investigate the growth and development laws of different mink varieties. The weights of six mink varieties were determined at different growth and development stages, respectively. The growth curves were drawn, and the differences in 150-day-old weight were analyzed. The results showed that the weight of each mink variety grew rapidly after weaning to adult weight; the weight of sliver blue mink was significantly higher than those of imported regal white mink and imported palomino mink; and there were no significant differences between other varieties.

关键词： Mink Weight Growth Development