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不同温拌剂类型对温拌沥青混合料性能的影响

山东交通科技 2022年第3期 91-93页

作者：冯秀亮山西路桥第八工程有限公司山西运城044000

为研究不同温拌剂对温拌沥青路用性能的影响,通过渗水试验、冻融劈裂试验、高温车辙试验以及弯曲小梁破坏试验和弯曲蠕变试验等,分别得到掺入不同温拌剂的沥青混合料的渗水系数、TSR值、动稳定度、最大拉应变以及劲度模量等。结果表明:... 详细信息

为研究不同温拌剂对温拌沥青路用性能的影响,通过渗水试验、冻融劈裂试验、高温车辙试验以及弯曲小梁破坏试验和弯曲蠕变试验等,分别得到掺入不同温拌剂的沥青混合料的渗水系数、TSR值、动稳定度、最大拉应变以及劲度模量等。结果表明:合成蜡会增大沥青混合料的渗水系数,而沸石与合成软蜡会减小渗水系数;各类温拌剂对水稳定性的影响有限,但都优于热拌沥青;掺量3%的合成蜡沥青混合料高温稳定性最好,其他温拌剂对高温稳定性都有适当提升;温拌剂的加入会对沥青混合料的低温抗裂性产生不利影响。

关键词：沥青混合料温拌剂渗水系数动稳定度 TSR

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三种方法对细胞周期诱导效率的比较

基因组学与应用生物学 2014年第4期33卷 756-760页

作者：冯秀亮刘淑娟段艳萍王爽郝俊严兴荣西北大学生命科学学院西安710069 南昌市医学科学研究所附属医院生殖医学科南昌330001 第四军医大学西京医院实验动物中心西安710032

比较两种处理方法对细胞周期调控的影响,本研究用饥饿法,胸腺嘧啶处理法和饥饿法结合胸腺嘧啶法诱导胎儿成纤维细胞进入G1/G0期。诱导两周后,收集3种方法来源的细胞用流式细胞技术、细胞活力检测、real-time PCR和核型分析检查细胞诱导... 详细信息

比较两种处理方法对细胞周期调控的影响,本研究用饥饿法,胸腺嘧啶处理法和饥饿法结合胸腺嘧啶法诱导胎儿成纤维细胞进入G1/G0期。诱导两周后,收集3种方法来源的细胞用流式细胞技术、细胞活力检测、real-time PCR和核型分析检查细胞诱导效率、细胞活力、成纤维细胞特异基因的表达水平和核型情况。结果表明,实验组细胞G1/G0期比率显著高于对照组(p0.05);FGF4、FGF2及Sox2的基因表达水平在各组间差异不显著(p>0.05)。这些结果表明,实验组3种方法对细胞特性没有显著影响,饥饿结合药物诱导可适当提高细胞诱导效率,为相关研究提供参考。

关键词：胎儿成纤维细胞细胞周期胸苷嘧啶

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酒精对小鼠颗粒细胞功能的影响（英文）

酒精对小鼠颗粒细胞功能的影响（英文）

陕西省毒理学会新时代环境与健康学术会议暨第四届会员代表大会

作者： Wanlu Xu Lihui Huo Jingjing Li Chunli Xu Shuang Wang Yanhong Yang Chuang Liu Xiaomin Zheng xiuliang feng Xingrong Yan Shaanxi Key Laboratory for Animal Conservation Key Laboratory of Resource Biology and Biotechnology in Western ChinaMinistry of EducationCollege of Life ScienceNorthwest University Department of Experimental Surgery of Xijing Hospital the Fourth Military Medical University Department of Obstetrics & Gynecology Tangdu Hospitalthe Fourth Military Medical University Key Laboratory of Fertility Preservation and Maintenance Ministry of Education Institute of Biomedicine PharmacologyBiomedicum HelsinkiUniversity of Helsinki

Alcohol is an important compound used in food,agriculture,and *** this study we investigated the effect of alcohol on oocyte quality in mice by exposing animals for different duration times during an estrous ***-oocyte complexes(COCs) were collected from mice following pregnant mare serum gonadotropin(PMSG)-and human chorionic gonadotropin(hCG)-induced *** number,E level in serum,and parthenogenetic embryo development in vitro were *** gene expression,mitochondrial membrane potential,and reactive oxygen species(ROS) levels in the cumulus were also *** results showed that acute exposure to alcohol did not affect ovulation time(p> 0.05).Blasocyst formation rate in vitro was significantly improved after 1 and 2 days of alcohol exposure(p <0.01).Mitochondrial membrane potential was significantly increased after 1-4 days of alcohol exposure(p <0.05),but decreased after 5 days(p <0.05).ROS levels remained relatively low after 2,3,and 4 days of exposure(p <0.05) and significantly increased after 6 days(p <0.05).In addition,Alcohol altered the expression of mitochondrial and nuclear genes in the *** together,our data suggest that acute exposure to alcohol affects oocyte quality by influencing the function and gene expression in the *** results underscore potential implications for the development of human reproductive therapeutics.

关键词： Oocyte quality alcohol mitochondria mice parthenogenesis

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高脂对小鼠颗粒细胞功能的影响（英文）

高脂对小鼠颗粒细胞功能的影响（英文）

Deep neural network algorithm for estimating maize biomass based on simulated Sentinel 2A vegetation indices and leaf area index

陕西省毒理学会新时代环境与健康学术会议暨第四届会员代表大会

作者： Jingjing Li Shuang Wang Bo Wang Chunli Xu Lihui Huo Wanlu Xu Jun hao Yanping Duan Xiaomin Zheng Jinlian Hua xiuliang feng Xingrong Yan College of life sciences in Northwest university Department of Experimental Surgery of Xijing Hospital Fourth Military Medical University Key Laboratory of Fertility Preservation and Maintenance Ministry of Education Institute of Biomedicine PharmacologyBiomedicum HelsinkiUniversity of Helsinki College of Veterinary Medicine Northwest A & F University

Human health has been compromised by obesity in the world,in which germ cell could be *** is one of specific germ cells,which supports the early embryonic development in *** investigate the relation between high fat diet and oocyte quality,mice were bred with high fat and normal diet to use as treated and control groups,***-oocyte complexs(COCs) were collected from treated and normal *** of COCs was observed by transmission electron *** level of genes for mitochondrial and nuclear functions in cumulus were identified by *** quality was evaluated by artificial *** results showed that the number of ovulated oocytes in treated group was more than control group(p<0.05).Efficiency of parthenogenetic embryonic development in vitro in treated group was also more than that of control group(p<0.05).The expression level of genes for mitochondrial functions in cumulus was lower than than of control(p<0.05),excluding CoxⅡ.E2 level in serum and expression level of p450 arom in treated group were higher than that of control(P<0.05).But lipoids in cumulus for treated group was significantly more than that of control group and the cristae of mitochondria was more clear in treated *** and ROS ’level for cumulus in treated group were higher than that of control group(p<0.05),and mitochondrial membrane potential level for cumulus in treated group was lower than that of control *** results demonstrated that parthenogenetic embryonic development in vitro could be improved by high fat diet,but mitochondrial functions were impaired,which will be used for references for human in diet intake.

关键词： mouse high fat diet oocyte parthenogenesis embryo obesity

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The Crop Journal 2020年第1期8卷 87-97页

作者： xiuliang Jin Zhenhai Li Haikuan feng Zhibin Ren Shaokun Li Institute of Crop Sciences Chinese Academy of Agricultural SciencesKey Laboratory of Crop Physiology and EcologyMinistry of AgricultureBeijing 100081China Beijing Research Center for Information Technology in Agriculture Beijing Academy of Agriculture and Forestry SciencesBeijing 100097China Northeast Institute of Geography and Agroecology Chinese Academy of SciencesChangchun 130102JilinChina

Accurate estimation of biomass is necessary for evaluating crop growth and predicting crop *** is also a key trait in increasing grain yield by crop *** aims of this study were(i)to identify the best vegetation indices for estimating maize biomass,(ii)to investigate the relationship between biomass and leaf area index(LAI)at several growth stages,and(iii)to evaluate a biomass model using measured vegetation indices or simulated vegetation indices of Sentinel 2A and LAI using a deep neural network(DNN)*** results showed that biomass was associated with all vegetation *** three-band water index(TBWI)was the best vegetation index for estimating biomass and the corresponding R2,RMSE,and RRMSE were 0.76,2.84 t ha−1,and 38.22%*** was highly correlated with biomass(R2=0.89,RMSE=2.27 t ha−1,and RRMSE=30.55%).Estimated biomass based on 15 hyperspectral vegetation indices was in a high agreement with measured biomass using the DNN algorithm(R2=0.83,RMSE=1.96 t ha−1,and RRMSE=26.43%).Biomass estimation accuracy was further increased when LAI was combined with the 15 vegetation indices(R2=0.91,RMSE=1.49 t ha−1,and RRMSE=20.05%).Relationships between the hyperspectral vegetation indices and biomass differed from relationships between simulated Sentinel 2A vegetation indices and *** estimation from the hyperspectral vegetation indices was more accurate than that from the simulated Sentinel 2A vegetation indices(R2=0.87,RMSE=1.84 t ha−1,and RRMSE=24.76%).The DNN algorithm was effective in improving the estimation accuracy of *** provides a guideline for estimating biomass of maize using remote sensing technology and the DNN algorithm in this region.

关键词： Biomass estimation Maize Vegetation indices Deep neural network algorithm LAI

卵巢动脉血流与卵母细胞回收数量和质量的关系

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陕西医学杂志 2010年第2期39卷 153-155页

作者：杨贵忠王颖冯秀亮王丽萍闫春芳王蕴颖赵静西安市第四医院生殖医学中心西安710004 第四军医大学西京医院实验外科

目的:探讨体外受精-胚胎移植(IVF/ICSI-ET)控制性超排卵中,卵巢动脉血流与回收卵母细胞数量及其质量的关系。方法:应用前瞻性研究法,随机选择164个超排卵周期,经阴道彩色多普勒B型超声,监测月经第2天(M2)和HCG日卵巢动脉血流S,D,PI、RI... 详细信息

目的:探讨体外受精-胚胎移植(IVF/ICSI-ET)控制性超排卵中,卵巢动脉血流与回收卵母细胞数量及其质量的关系。方法:应用前瞻性研究法,随机选择164个超排卵周期,经阴道彩色多普勒B型超声,监测月经第2天(M2)和HCG日卵巢动脉血流S,D,PI、RI和S/D。分析回收卵母细胞数和成熟卵母细胞数与卵巢动脉血流之间的关系。结果:164个超排卵(COH)周期共回收卵母细胞1 714枚,其中成熟卵母细胞1 334枚。根据回收卵母细胞数量和成熟卵母细胞数量分别分成4个组,M2和HCG日,不同回收卵母细胞数4个组的PI、RI和S/D值的波动范围是一致;同样不同回收成熟卵母细胞数4个组的PI、RI和S/D值的波动范围也是一致。但是随着回收卵母细胞数或成熟卵母细胞数的增加,M2和HCG日卵巢动脉血流PI、RI和S/D逐渐降低。结果显示,M2日,当PI<0.62、RI<0.48者,超排卵后可能回收到更多的卵母细胞;而PI<0.60、RI<0.46者,超排卵后可能获得更多的成熟卵母细胞。结论:在COH周期中,卵巢动脉血流与回收卵母细胞数和成熟数密切相关;M2和HCG日,卵巢动脉血流PI、RI值可作为评估超排卵结果的有效指标。

关键词：卵巢动脉血流速度局部血流卵母细胞

Omicron:a chimera of two early SARS-CoV-2 lineages

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Signal Transduction and Targeted Therapy 2022年第4期7卷 970-973页

作者： xiuliang Liu Jiasheng Xiong Zhong Sun Jingjing Hu Karuppiah Thilakavathy Mingquan Chen Qi Zhao Yi feng Qingwu Jiang Chenglong Xiong Department of Epidemiology School of Public HealthKey Laboratory of Public Health SafetyMinistry of EducationFudan University200032 ShanghaiChina Department of Social Medicine School of Public HealthKey Laboratory of Public Health SafetyMinistry of EducationFudan University200032 ShanghaiChina Department of Biomedical Science Faculty of Medicine and Health SciencesUniversiti Putra MalaysiaSerdang 43400Malaysia Shanghai Pinnacles Medical Technology Co. Ltd200126 ShanghaiChina Genetics and Regenerative Medicine Research Group Faculty of Medicine and Health SciencesUniversiti Putra MalaysiaSerdang 43400Malaysia Department of Emergency Huashan HospitalFudan University200040 ShanghaiChina Department of Integrative Medicine and Neurobiology School of Basic MedicalSciencesInstitutes of Brain ScienceBrain Science Collaborative Innovation CenterState Key Laboratory of Medical NeurobiologyInstitute of Acupuncture and MoxibustionFudan Institutes of Integrative MedicineFudan University200032 ShanghaiChina

Dear Editor,The outbreak of the COVID‐19 that occurred in late 2019 has posed a remarkable threat to public health around the *** is known that SARS-CoV-2 is a genetically diverse group that mutates continuously,leading to the emergence of multiple variants.1 Potential variants of concern(VOCs),variants of interest(VOIs),or variants under monitoring(VUMs)are regularly assessed based on the risk posed to global public *** the identification of a novel variant in South Africa on 24 November 2021,WHO designated Omicron(clade GRA,PANGO lineage B.1.1.529 and descendants BA.1 and BA.2)as the fifth SARS-CoV-2 VOC 2 days later due to its large number of variations.

关键词： threat has continuously

无人机观测时间对玉米冠层叶绿素密度估算的影响

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浙江农业学报 2024年第1期36卷 18-31页

作者：周丽丽冯海宽聂臣巍许晓斌刘媛孟麟薛贝贝明博梁齐云苏涛金秀良安徽理工大学空间信息与测绘工程学院安徽淮南232001 中国农业科学院作物科学研究所北京100081 中国农业科学院国家南繁研究院海南三亚572024 国家农业信息化工程技术研究中心北京100097 武汉大学测绘与遥感信息工程国家重点实验室湖北武汉430079

为探讨不同时间获取的无人机多光谱数据对玉米冠层叶绿素密度(canopy chlorophyll density,CCD)估算的影响,分别在玉米抽雄吐丝期、籽粒建成期、乳熟期和蜡熟期选择同一天的10:00—10:59、11:00—11:59、13:00—13:59和14:00—14:59进... 详细信息

为探讨不同时间获取的无人机多光谱数据对玉米冠层叶绿素密度(canopy chlorophyll density,CCD)估算的影响,分别在玉米抽雄吐丝期、籽粒建成期、乳熟期和蜡熟期选择同一天的10:00—10:59、11:00—11:59、13:00—13:59和14:00—14:59进行无人机多光谱观测试验,并结合PROSAIL模型模拟结果与实测CCD数据,分析一天中不同时刻典型植被指数的变化规律及CCD估算结果的差异。结果表明:在同一天中,无人机玉米冠层反射率和与实测CCD相关性较好的植被指数值均随时间变化,近红外波段的反射率变化最明显,越接近12:00,实测的植被指数值越低,而在一天的不同时间PROSAIL模型模拟的植被指数值几乎没有差异。在同一天,基于不同观测时间获取的同一植被指数与实测CCD的相关性存在较大差异,且不同生育时期和不同指数间的差异不一致;而模拟得到的同一植被指数与CCD的相关性在同一天不同时间的差异不明显。在不同生育时期,基于不同观测时间无人机数据构建的CCD估算模型均可以取得较好的精度,但不同观测时间的估算结果存在差异,决定系数最低的为0.53,最高的为0.80。这些结果表明,在传统的光谱数据获取时间范围内(10:00—14:00),无人机影像获取时间仍对玉米CCD估算有影响,越接近12:00,估算精度越高。研究结果可为后续作物的CCD精准估算提供基础支撑。

关键词：冠层叶绿素密度观测时间机器学习 PROSAIL模型玉米

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Optimization of microbial cell factories for astaxanthin production: Biosynthesis and regulations, engineering strategies and fermentation optimization strategies

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Synthetic and Systems Biotechnology 2022年第2期7卷 689-704页

作者： Mostafa Basiony Liming Ouyang Danni Wang Jiaming Yu Liming Zhou Mohan Zhu Xuyuan Wang Jie feng Jing Dai Yijie Shen Chengguo Zhang Qiang Hua xiuliang Yang Lixin Zhang State Key Laboratory of Bioreactor Engineering East China University of Science and TechnologyShanghai200237China Shandong Jincheng Bio-Pharmaceutical Co. Ltd.No.117 Qixing River RoadZibo255130ShandongChina

The global market demand for natural astaxanthin is rapidly increasing owing to its safety,the potential health benefits,and the diverse applications in food and pharmaceutical *** major native producers of natural astaxanthin on industrial scale are the alga Haematococcus pluvialis and the yeast Xanthopyllomyces ***,the natural production via these native producers is facing challenges of limited yield and high cost of cultivation and ***,astaxanthin production via metabolically engineered non-native microbial cell factories such as Escherichia coli,Saccharomyces cerevisiae and Yarrowia lipolytica is another promising strategy to overcome these *** this review we summarize the recent scientific and biotechnological progresses on astaxanthin biosynthetic pathways,transcriptional regulations,the interrelation with lipid metabolism,engineering strategies as well as fermentation process control in major native and non-native astaxanthin *** progresses illuminate the prospects of producing astaxanthin by microbial cell factories on industrial scale.

关键词： Astaxanthin Carotenoids Metabolic engineering Microbial cell factories Lipid Fermentation Antioxidants