高脂对小鼠颗粒细胞功能的影响（英文）
作者单位：College of life sciences in Northwest university Department of Experimental Surgery of Xijing HospitalFourth Military Medical University Key Laboratory of Fertility Preservation and MaintenanceMinistry of Education Institute of BiomedicinePharmacologyBiomedicum HelsinkiUniversity of Helsinki College of Veterinary MedicineNorthwest A ＆ F University
会议名称：《陕西省毒理学会新时代环境与健康学术会议暨第四届会员代表...》
会议日期：2018年
学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学]
关 键 词：mouse high fat diet oocyte parthenogenesis embryo obesity
摘 要：Human health has been compromised by obesity in the world,in which germ cell could be *** is one of specific germ cells,which supports the early embryonic development in *** investigate the relation between high fat diet and oocyte quality,mice were bred with high fat and normal diet to use as treated and control groups,***-oocyte complexs（COCs） were collected from treated and normal *** of COCs was observed by transmission electron *** level of genes for mitochondrial and nuclear functions in cumulus were identified by *** quality was evaluated by artificial *** results showed that the number of ovulated oocytes in treated group was more than control group（p0.05）.Efficiency of parthenogenetic embryonic development in vitro in treated group was also more than that of control group（p0.05）.The expression level of genes for mitochondrial functions in cumulus was lower than than of control（p0.05）,excluding CoxⅡ.E2 level in serum and expression level of p450 arom in treated group were higher than that of control（P0.05）.But lipoids in cumulus for treated group was significantly more than that of control group and the cristae of mitochondria was more clear in treated *** and ROS ’level for cumulus in treated group were higher than that of control group（p0.05）,and mitochondrial membrane potential level for cumulus in treated group was lower than that of control *** results demonstrated that parthenogenetic embryonic development in vitro could be improved by high fat diet,but mitochondrial functions were impaired,which will be used for references for human in diet intake.