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Frontiers of Environmental Science & Engineering 2016年第4期10卷 127-136页

作者： Jiaxuan YANG Jun MA Dan SONG Xuedong ZHAI xiujuan kong State Key Laboratory of Urban Water Resource and Environment Harbin Institute of Technology Harbin 150090 China

The combination of preozonation and subsequent biological granular activated carbon （O3/BAC） filtration is well known as a promising method for the removal of many pollutants. Temperature and nutrients are the dominant factors in external conditions to influence the biological communities. To explore the influence of preozonation under low temperature, the factors such as dissolved oxygen （DO）, dissolved organic carbon （DOC） and NH4^＋-N were analyzed from the sampling ports every week; triphenyl tetrazolium ehloride-dehydrogenase activity （TTC-DHA） and the nitrifying activity were detected along the bed height ofbiofilter at four levels （10. 40. 70 and i 10 cm） on the 90th, 110th, and 130th day; ~nicrobial community, based on 16S rRNA gene-denaturing gradient gel electrophoresis （DGGE）, was monitored on the 130th day of the operation. The observed microbial property showed that preozonation had a positive influence on bioactivity, biomass and nitrifying activity. Community analysis showed no significant difference on the biodiversity of nitrifying bacteria between the parallel filters in the inlet end based on the method employed. This result showed that biofilters＇ performance is not correlated well with microbial biodiversity. The elevated functionality in O3/BAC filters can be a result of increased microbial activity, which was promoted by preozonation.

关键词： Preozonation BAC filtration High ammonia level Bacterial activity Microbial community structurePilot scale study

Rap2-induced migration of renal cancer cell Caki-1 is mediated by JNK

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Rap2-induced migration of renal cancer cell Caki-1 is mediat...

Molecular epidemiology demonstrates that imported and local strains circulated during the 2014 dengue outbreak in Guangzhou, China

中国生物化学与分子生物学会第十届会员代表大会暨全国学术会议

作者： xiujuan kong Shuliang Shi The Department of Life Science and Engineering Harbin Institute of Technology

Rap2,a Ras family small GTPase regulates a variety of cellular functions including cell proliferation,adhesion and migration through activating its downstream effectors Raf,MAP4K4,TNIK,PARG,*** these factors, MAP4K4,a member of STE20 family kinase is involved in the regulation of cell migration of multiple cancer cell lines. JNK,a downstream target regulated by MAP4K4 is found to correlate to the promoted migration of many carcinoma *** quiescent states,the inactivated JNK distributes over cytoplasm and nucleus,and once activated it translocates to the nucleus as well as to the plasma membrane,regulating

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Virologica Sinica 2017年第1期32卷 63-72页

作者： Geng Li Pan Pan Qiuyan He xiujuan kong Kailang Wu Wei Zhang Yuntao Liu Huiting Huang Jianbo Liu Zhongde Zhang De Wu Xiaoping Lai Xiaohong Liu Jianguo Wu School of Chinese Meterla Medica Guangzhou University of Chinese Medicine Guangzhou 510006China State Key Laboratory of Virology College of Life Sciences Wuhan University Wuhan 430072 China The First Affiliated Hospital of Guangzhou University of Chinese Medicine Guangzhou 510120 China Guangdong Province Traditional Chinese Medical Hospital Guangzhou 510120 China Guangdong Provincial Center for Disease Control and Prevention Guangzhou 511430 China

The dengue virus （DENV） is a vital global public health issue. The 2014 dengue epidemic in Guangzhou, China, caused approximately 40,000 cases of infection and five deaths. We carried out a comprehensive investigation aimed at identifying the transmission sources in this dengue epidemic. To analyze the phylogenetics of the 2014 dengue strains, the envelope （E） gene sequences from 17 viral strains isolated from 168 dengue patient serum samples were sequenced and a phylogenetic tree was reconstructed. All 17 strains were serotype I strains, including 8 genotype I and 9 genotype V strains. Additionally, 6 genotype I strains that were probably introduced to China from Thailand before 2009 were widely transmitted in the 2013 and 2014 epidemics, and they continued to circulate until 2015, with one affinis strain being found in Singapore. The other 2 genotype I strains were introduced from the Malaya Peninsula in 2014. The transmission source of the 9 genotype V strains was from Malaysia in 2014. DENVs of different serotypes and genotypes co-circulated in the 2014 dengue outbreak in Guangzhou. Moreover, not only had DENV been imported to Guangzhou, but it had also been gradually exported, as the viruses exhibited an enzootic transmission cycle in Guangzhou.

关键词： dengue virus (DENV) phylogenetic analysis envelope (E) gene enzootic transmission cycle

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紫外-氯对细菌的协同消毒效果

水处理技术 2016年第1期42卷 41-44页

作者：孔秀娟马军文刚肖佳月哈尔滨工业大学市政与环境工程学院黑龙江哈尔滨150090 西安建筑科技大学环境与市政工程学院陕西西安710055

研究了紫外-氯的联合作用对大肠杆菌和芽孢的消毒效能。相对于单独紫外消毒,紫外-氯能显著提高芽孢的消毒效率而对大肠杆菌的消毒效果影响不大。大肠杆菌对紫外和氯均比较敏感,而芽孢则需较高辐照剂量的紫外或氯才能达到相同的消毒效果... 详细信息

研究了紫外-氯的联合作用对大肠杆菌和芽孢的消毒效能。相对于单独紫外消毒,紫外-氯能显著提高芽孢的消毒效率而对大肠杆菌的消毒效果影响不大。大肠杆菌对紫外和氯均比较敏感,而芽孢则需较高辐照剂量的紫外或氯才能达到相同的消毒效果。紫外-氯较紫外-过氧化氢能更有效地产生羟基自由基,但当氯离子浓度较高时部分羟基自由基与之反应生成氯自由基。紫外-氯对芽孢消毒的协同作用可部分归因于在此过程中产生的羟基自由基及形成的氯自由基。

关键词：消毒紫外-氯自由基

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地黄丸类方对肾阳虚大鼠HPA轴的影响

中药新药与临床药理 2015年第3期26卷 320-324页

作者：李耿张喆尹西拳孔秀娟许仕杰吴伊娜林吉广州中医药大学广东广州510006

目的比较桂附、知柏、六味地黄丸方对肾阳虚大鼠治疗作用的异同以及对下丘脑-垂体-肾上腺轴(HPA)的影响。方法取SD大鼠80只,随机分为8组(每组10只):正常组,模型组,六味地黄丸高、低剂量组(4,1 g·kg-1),知柏地黄丸高、低剂量组(4,1 g·k... 详细信息

目的比较桂附、知柏、六味地黄丸方对肾阳虚大鼠治疗作用的异同以及对下丘脑-垂体-肾上腺轴(HPA)的影响。方法取SD大鼠80只,随机分为8组(每组10只):正常组,模型组,六味地黄丸高、低剂量组(4,1 g·kg-1),知柏地黄丸高、低剂量组(4,1 g·kg-1),桂附地黄丸高、低剂量组(4,1 g·kg-1),灌胃给药,连续21 d。采用氢化可的松注射液肌注14 d的方法,制备大鼠肾阳虚模型,观察3种地黄丸方对肾阳虚大鼠的一般状态、体质量、肛温、血浆cAMP、cGMP、CRH、ACTH、血清CORT含量以及血浆cAMP/cGMP水平的影响。结果建模后大鼠体质量、肛温均有显著的降低,血浆cAMP水平无显著性变化,cGMP水平则显著升高,c AMP/c GMP比值显著下降,血浆CRH、ACTH和血清CORT水平均较正常组显著下降(P0.05);桂附地黄丸可显著升高cAMP/cGMP比值和恢复大鼠HPA轴功能(P<0.05,P<0.01),六味、知柏地黄丸组均无显著性改变。结论 3种地黄丸类方对肾阳虚大鼠生存状态及神经内分泌网络的影响方面存在明显差异,桂附地黄丸可以明显改善肾阳虚大鼠HPA轴抑制的状态;六味地黄丸对于肾阳虚大鼠免疫功能表现出一定的改善作用,知柏地黄丸改善作用不明显。

关键词：地黄丸类方肾阳虚 cAMP cGMP CRH ACTH CORT

Nitrogen-oxygen co-doped corrugation-like porous carbon for high performance supercapacitor

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Frontiers of Materials Science 2018年第3期12卷 283-291页

作者： Wang YANG Wu YANG Lina kong Shuanlong DI xiujuan QIN Hebei Key Laboratory of Applied Chemistry College of Environmental and Chemical Engineering Yanshan University Qinhuangdao 066004 China State key Laboratory of Metastable Materials Science and Technology Yanshan University Qinhuangdao 066004 China

Nitrogen-oxygen co-doped corrugation-like porous carbon （NO-PC） has been developed by direct pyrolysis of formaldehyde-melamine polymer containing manganese nitrate. The melamine, formaldehyde and manganese nitrate act as nitrogen, oxygen source and pore-foaming agent, respectively. NO-PC exhibits favorable porous architecture for efficient ion transfer and moderate heteroatom doping for additional pseudocapacitance, which synergistically enhances the electrochemical performance of the NO-PC-based supercapacitor. The electrode delivers specific capacitance of 240 Fig at 0.3 A/g when tested in 6 mol/L KOH electrolyte, good rate capability （capacitance retention of 83.3% at 5 A/g） as well as stable cycling performance （capacitance remains -96% after 10000 cycles at 3 A/g）. The facile synthesis with unique architecture and chemistry modification offers a promising candidate for electrode material of energy storage devices.

关键词： nitrogen-oxygen co-doping porous carbon supercapacitor

Research in migration route of hatchery released Chinese shrimp(Fenneropenaeus chinensis) in the Bohai Bay using method of SSR marker

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Acta Oceanologica Sinica 2020年第12期39卷 76-81页

作者： Weiji Wang Ding Lyu Mosang Wang Kefeng Liu Jie kong xiujuan Shan Xianshi Jin Key Laboratory of Sustainable Development of Marine Fisheries of Ministry of Agriculture Yellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdao 266071China Laboratory for Marine Fisheries Science and Food Production Processes Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266237China Bohai Fisheries Research Institute of Tianjin Tianjin 300457China

This study provides new insights for the hatchery released Chinese shrimp(Fenneropenaeus chinensis),including proportion,dynamic migration route,after they were released into nature for stock enhancement using a new strategy quite different than *** shrimp were sampled at 22 survey stations during two investigation voyages acrossing 74 survey stations in the Bohai Sea from July 16 to August 9 in *** 289 sampled individuals during the second voyage,totally 155 shrimps were identified as hatchery shrimp released into the Laizhou Bay at mid-May in 2015 based on finger-print of eight SSR(simple sequence repeats)markers,and the proportion of hatchery released shrimp in recapture samples were from 41.30%–85.71%in each station with an average value 53.63%,which verified a previous view point that up to 90%of autumn season Chinese shrimp landing in the Bohai Sea were composed of hatchery ***,the dynamic migration route of hatchery released shrimp revealed that part of released shrimp migrated heading northwest along the west coast of the Bohai Sea up to the Bohai Bay but just remained at the Laizhou Bay until over-wintering migration at midOctober when they initiate over-wintering *** unnatural spring season shrimp fishing model cut the throat of spawner shrimp chance to swim back to their respective spawning plants at each spring,it still no chance to clarify whether the hatchery released shrimp could replenish to the reproduce population and complete a whole life cycle as same as their natural relatives.

关键词： Fenneropenaeus chinensis migration route hatchery release stock enhancement SSR marker

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地黄丸类方对肾阳虚大鼠免疫功能的影响

广东药学院学报 2015年第5期31卷 629-632页

作者：吴依娜尹西拳孔秀娟张蓓邱晶晶赖小平李耿广州中医药大学中药学院广东广州510006

目的观察3种地黄丸类方对肾阳虚大鼠免疫功能的影响,并从基因水平探讨其免疫调节的机制。方法采用氢化可的松注射液肌注2周造成肾阳虚大鼠模型,造模成功后,六味、桂附、知柏地黄丸连续灌胃给药3周,应用酶联免疫法(ELISA)检测各组大鼠血... 详细信息

目的观察3种地黄丸类方对肾阳虚大鼠免疫功能的影响,并从基因水平探讨其免疫调节的机制。方法采用氢化可的松注射液肌注2周造成肾阳虚大鼠模型,造模成功后,六味、桂附、知柏地黄丸连续灌胃给药3周,应用酶联免疫法(ELISA)检测各组大鼠血清IL-2及IL-4的含量,荧光定量PCR法检测各组大鼠肾组织NF-κB mRNA表达水平。结果与正常组比较,模型组胸腺质量显著下降,血清IL-2、IL-4水平显著改变(P0.05);与模型组比较,六味、桂附地黄丸各组均可显著降低大鼠肾组织NF-κB mRNA表达量(P0.05)。结论六味地黄丸、桂附地黄丸对于肾阳虚大鼠免疫功能有一定的改善作用,知柏地黄丸改善作用不显著。

关键词：氢化可的松地黄丸类方免疫 NF-κB通路

红花提取物对Ⅰ型及Ⅱ型5α-还原酶的抑制活性研究

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中国药业 2015年第14期24卷 17-20页

作者：邓桂球张蓓孔秀娟张榕文劳梓钊万玉华李耿广东省广州市白云区第一人民医院广东广州510140 广州中医药大学广东广州510405 霸王<广州>有限公司广东广州510006

目的研究红花提取物对Ⅰ型、Ⅱ型5α-还原酶的抑制活性。方法基于高效液相色谱(HPLC)法,建立抑制5α-还原酶体外模型,从雌性大鼠肝脏、雄性大鼠附睾组织中提取Ⅰ型、Ⅱ型5α-还原酶,与底物睾酮(T)及供氢体还原型辅酶Ⅱ(NADPH)共同组成... 详细信息

目的研究红花提取物对Ⅰ型、Ⅱ型5α-还原酶的抑制活性。方法基于高效液相色谱(HPLC)法,建立抑制5α-还原酶体外模型,从雌性大鼠肝脏、雄性大鼠附睾组织中提取Ⅰ型、Ⅱ型5α-还原酶,与底物睾酮(T)及供氢体还原型辅酶Ⅱ(NADPH)共同组成了一种微量的酶促反应体系。采用HPLC法测定反应前后T含量,以此判断5α-还原酶的活性。非那雄胺作阳性对照药。结果与正常组比较,红花提取物0.1 g/L剂量组Ⅰ型、Ⅱ型酶活力,T转化率均无显著性差异(P>0.05);0.1,0.5,2.5 g/L剂量组酶活力、T转化率均显著降低(P<0.01)。结论红花提取物对Ⅰ型、Ⅱ型5α-还原酶活性均有一定的抑制作用,可为临床治疗前列腺增生、雄激素脱发等疾病提供参考。

关键词： 5α-还原酶雄激素雄激素脱发前列腺增生高效液相色谱法

中药育发液对表皮角质形成细胞体外增殖作用的影响

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国际医药卫生导报 2018年第15期24卷 2237-2240页

作者：林壮民孔秀娟中小花吴庆光张玲玲张榕文李耿吴依娜广东省第二人民医院药学部广州510317 广州中医药大学 510006 霸王(广州)有限公司 510000

目的研究中药育发液对表皮角质形成细胞（HEK）的体外增殖作用的影响。方法以章光101育发液为阳性药，通过用酶消化法培养HEK，观察形态，采用MTF法测定细胞生长活力，绘制各组细胞的生长曲线，以评价中药育发液对HEK体外增殖作用的影... 详细信息

目的研究中药育发液对表皮角质形成细胞（HEK）的体外增殖作用的影响。方法以章光101育发液为阳性药，通过用酶消化法培养HEK，观察形态，采用MTF法测定细胞生长活力，绘制各组细胞的生长曲线，以评价中药育发液对HEK体外增殖作用的影响。结果与空白对照组比较，给药24h后．中药育发液组细咆数量增多，细胞间有部分相互连接，胞浆内没有透明空泡，细胞体积均匀，细胞生长状态良好；MTF结果显示，中药育发液各剂量组的OD值显著升高（P〈0．01），且对表皮角质形成细胞增殖的促进率均在40％以上。结论中药育发液各剂量组在体外可显著促进HEK的增殖。

关键词：中药育发液表皮角质形成细胞 MTT