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Geochronological and geochemical constraints on the Cuonadong leucogranite, eastern Himalaya

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Acta Geochimica 2018年第3期37卷 347-359页

作者： Jiajia Xie Huaning Qiu xiujuan bai Wanfeng Zhang Qiang Wang Xiaoping Xia State Key Laboratory of Isotope Geochemistry Guangzhou Institute of Geochemistry Chinese Academy of SciencesGuangzhou 510640 China Key Laboratory of Tectonics and Petroleum Resources(China University of Geosciences Wuhan) Ministry of Education Wuhan 430074 China University of Chinese Academy of Sciences Beijing 100049China

First comprehensive investigations of the Cuonadong leucogranite exposed in North Himalayan gneiss dome of southern Tibet are presented in this *** SIMS U–Pb ages of oscillatory zircon rims scatter in a wide range from 34.1 to 16.0 Ma, and the Cuonadong leucogranite probably emplaced at 16.0 Ma. High-precision ^(40)Ar/^(39)Ar dating on a muscovite sample yields an essentially flat age spectrum with consistent plateau and isochron ages, indicating that the Cuonadong leucogranite cooled below 450 °C at 14 Ma. Based on the youngest zircon U–Pb age and muscovite^(40)Ar/^(39)Ar age, the Cuonadong leucogranite experienced rapid cooling with a rate of 119 °C/Myr from 16 to 14 Ma. The geochronological data of this undeformed leucogranite also suggest that the ductile extension of the South Tibetan Detachment System in the eastern Himalaya ceased by ca. 14 Ma. Furthermore,the initial Sr–Nd isotopic compositions and Nd model ages demonstrate that the leucogranite was derived from metapelitic source within the Greater Himalayan Crystalline Complex. The distinct Ba depletion with high Rb/Sr ratios and negative Eu anomalies make it clear that the leucogranite melts were generated by breakdown of muscovite under fluid-absent conditions.

关键词： Leucogranite SIMS zircon U-Pb dating 40Ar/39Ar dating Geochemistry North Himalaya

Decoupled Ce-Nd Isotopic Systematics of the Neoproterozoic Huangling Intrusive Complex and Its Geological Significance, Eastern Three Gorges, South China

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Journal of Earth Science 2016年第5期27卷 864-873页

作者： Yongjuan Gao Wenli Ling Xiaofei Qiu Ziwan Chen Shansong Lu Xiao bai xiujuan bai Junbo Zhang Hongmei Yang Ruichun Duan Chengdu Institute of Geology and Mineral Resource Chengdu 610081 China State Key Laboratory of Geological Progresses and Mineral Resources China University of Geosciences Wuhan 430074 China School of Earth Sciences China University of Geosciences Wuhan 430074 China Wuhan Center of Geological Survey Wuhan 430205 China

Cerium is one of multivalent rear earth elements, which can transfer from trivalence to tretavalence at oxidizing environment. This process may cause variable degrees of fractionation of Ce from other trivalent rear earth elements, and thus may provide specific insight into the geological processes associated with marked redoxomorphism. Multiple geochemical tracing of Sr-Nd-Ce isotopes are performed on the felsic and mafic intrusives of the Neoproterozoic（~800 Ma） Huangling complex located at the eastern Three Gorges, South China. The intrusive rocks exclusively show various extents of negative Ce anomalies. On the εCe-εNd plot, most samples from the mafic intrusions scatter within the second quadrant, whereas those from the felsic intrusions within the fourth Quadrant. Both of the two groups exhibit relatively large range of ？Ce（t） variation but limited ？Nd（t） range, which cause a deviation from the ＂crustal array＂ and reveal a decoupled Nd-Ce isotope correlation. The intermediate-felsic suite have varied Ce/Ce＊ ratios but broadly proximate εCe（t） values, indicating that their negative Ce anomalies were generated during the magmatism; on the contrary, a positive correlation between εCe（t） and Ce/Ce＊ is observed for the intermediate-mafic suite, an indication of an origin of post-magmatic alteration or metamorphism for their Ce anomalies. Calculation of model age, the occurrence age of negative Ce anomalies（TCe） for the intermediate-mafic samples infers that the alteration events took place 〉350 Ma. Data showed that negative Ce anomalies of the felsic intrusions may reflect an increase of oxygen fugacity during magma ascending, rather than an inheritance from their source rocks. This explanation implies that the Neoproterozoic magmatism occurred at the continental nucleus of the Yangtze block were developing at a geodynamic context of rapidly regional uplifting.

关键词： negative Ce anomalies Ce-Nd isotopic decoupling alteration oxidation environment.

Differentiation potential of bone marrow mesenchymal stem cells in duck

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Journal of Genetics and Genomics 2009年第3期36卷 133-140页

作者： Linfeng Li xiujuan bai Xuelian Gong Hongkun Liu Lina Chen Weijun Guan Yuehui Ma Institute of Animal Science Chinese Academy of Agricultural Science Beijing 100193 China College of Animal Science and Technology Northeast Agricultural University Harbin 150000 China

The bone marrow mesenchymal stem cells （MSCs） are multipotent stem cells which can differentiate into mesenchymal cells in vitro. In this study, MSCs in duck were isolated from bone marrow by density gradient centrifuge separation, purified and expanded in the me- dium. The primary MSCs were expanded for 11 passages. The different-passage MSCs were induced to differentiate into osteoblasts and neuron-like cells. Karyotype analysis indicated that MSCs kept diploid condition and the hereditary feature was stable. The different- passage MSCs expressed CD44, ICAM-1 and SSEA-4, but not CD34, CD45 and SSEA-1 when detected by immunofluorescence staining There was no significant difference among the positive rates of passages 2, 6 and 8 （P 〉 0.05）, but a significant difference existed among those of passages 2, 6, 8 and 11 （P 〈 0.05）. After the osteogenic inducement was added, the induced different-passage MSCs expressed high-level alkaline phosphatase （ALP）, and are positive for tetracycline staining, Alizarin Red staining and Von Kossa staining. After the neural inducement was added, about 70% cells exhibited typical neuron-like phenotype, the induced different-passage MSCs expressed Nestin, neuron-specific enolase （NSE） and glial fibrillary acidic protein （GFAP） when detected by immunofluorescence staining. There was no significant difference among the positive rates of passages 3, 4 and 6 （P〉0.05）, but a significant difference existed among those of passages 3, 4, 6 and 8 （P〈0.05）. These results suggest that MSCs in duck were capable of differentiating into osteoblasts and neuron-like cells in vitro.

关键词： duck mesenchymal stem cells induction differentiation

Primary Analysis on mtDNA D-loop Hypervariable Region in Eutamias sibiricus

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Journal of Northeast Agricultural University(English Edition) 2008年第1期15卷 48-50页

作者： LI Shipeng bai xiujuan College of Animal Science and Technology Northeast Agricultural University Harbin 150030 China

This study analyzed the mitochondrial DNA D-loop hypervariable region 601 bp sequence in 12 Eutamias sibiricus from Heilongjiang area. The result showed that the average contents of A, T, G and C were 33.2%, 30.5%, 11.8% and 24.5% respectively, the A＋T content （63.7%） was obviously higher than the G＋C content （36.3%）. Thirty-six, mutation （approximately 6.0%） sites were found and 9 haplotypes were defined. The mutations types, including transition, transversion and deletion were all found in the detected mtDNA D-loop regions, most of which was transition. The average nucleotide mutational ratio was 1.22%. The nucleotide mutation sites affected the restriction site appearance or disappearance of the restriction site. The research on mtDNA D-loop is focused on the domestic animals and there is no report on Eutamias sibiricus, This study analyzed the mitochondrial DNA D-loop hypervariable in Eutamias sibiricus so as to provide some useful informations for related research in the future.

关键词： Eutamias sibiricus mtDNA D-loop hypervariable region genetic diversity

The Genetic Diversity Study of Wild Wusuli Raccoon Dog From Nenjiang District

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Journal of Northeast Agricultural University(English Edition) 2008年第3期15卷 20-24页

作者： YANG Chunshan bai xiujuan College of Animal Science and Technology Northeast Agricultural University Harbin 150030 China

The ISSR method was developed to analyze the genetic diversity of 20 wild Wusuli raccoon dog from Nenjiang district. The results showed that there were significant genetic diversity and high polymorphism among individuals of the wild Wusuli raccoon dog. A total of 41 DNA bands were amplified by 9 primers, and 37 of them were polymorphism, the proportion of polymorphism was 90.24%, 2-8 polymorphism bands could be amplified by each primer, and 4.56 on average, the length of the product was 200-2 000 bp.

关键词： Wusuli raccoon dog ISSR genetic diversity

Male heterogametic sex determination in Rana dybowskii based on sex-linked molecular markers

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Integrative Zoology 2022年第1期17卷 105-114页

作者： Yuan XU Zhiheng DU Jiayu LIU Hang SU Fangyong NING Shiquan CUI Lijuan WANG Jianming LIU Chuanshuai REN Shengwei DI xiujuan bai College of Animal Science and Technology Northeast Agricultural UniversityHarbinChina College of Life Sciences Northeast Agricultural UniversityHarbinChina College of Agricultural Eastern Liaoning UniversityDandongChina Yili Animal Science Research Institute YiningChina Animal Husbandry Administration of Huadian HuadianChina Branch of Animal Husbandry and Veterinary of Heilongjiang Academy of Agricultural Sciences QiqiharChina

Identifying the mechanism for sex determination in amphibians is *** little is known about sex determination mechanisms of Rana dybowskii,a species of importance to evolutionary and conservation *** screened for sex-linked molecular markers in *** in China using target region amplification polymorphism with 2 fixed primers against the sequences of *** found 2 male-linked molecular markers in ***,which were 222 bp and 261 bp *** detection rates of 222 bp marker in males form Xinglong,Huadian,and Dandong were 93.79%,69.64%,and 13.64%,respectively,while the rate in females from Huadian was 27.50%.Besides,the detection rates of 261 bp marker in the above 3 regions were only observed in males at the rate of 93.79%,87.50%,and 32.73%,*** inheritance patterns of sex-linked molecular markers showed that the 2 sex-linked molecular markers were *** to the XY-male parent,progeny from XX-pseudo-male parent possessed lower sex reversal ratio at the same rearing temperature,and the proportion of female froglets from an XX-pseudo-male parent was more than 95%at low rearing temperature(15°C).Our findings suggest that *** displays male heterogamety,and the 2 sex-linked molecular markers may have a guiding significance for the protection and utilization of ***.

关键词： male-linked molecular markers Rana dybowskii sexual reversal TRAP markers

A novel purification technique for noble gas isotope analyses of authigenic minerals

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Science China Earth Sciences 2016年第10期59卷 111-117页

作者： HE LiYan QIU HuaNing SHI HeSheng ZHU JunZhang bai xiujuan YUN JianBing State Key Laboratory of Isotope Geochemistry Guangzhou Institute of GeochemistryChinese Academy of SciencesGuangzhou 510640China Exploration Department of CNOOC Ltd. Beijing 100010China CNOOC Limited-Shenzhen Shenzhen 518067China Key Laboratory of Tectonics and Petroleum Resources of Ministry of Education China University of GeosciencesWuhan 430074China Exploration Department of Daqing Oilfield Co.Ltd. Daqing 163712China

Noble and active gases are released from geological samples during gas extraction for noble gas isotope analyses. The active gases should be removed before inletting to mass spectrometers for the analyses. The normal noble gas preparation systems can clean up most geological samples. However, authigenic minerals from sedimentary rocks in oil/gas fields contain organic matter, which cannot be cleaned up by the normal preparation systems and thus influence the noble gas analyses. We introduce a novel gas purification system （PRC patent No. ZL201320117751.2）, which includes several reversible purification pumps with different absorbing and degassing temperatures. It can well clean up water steam, carbon dioxide and organic gas- es. Mica minerals are often used for 40Ar/39Ar dating. A muscovite sample （2082MS） which could not be cleaned up by the normal preparation system with two SAES NP10 getters, becomes the test sample for a comparative experiment in this study. The experiment is assigned into 4 sections with the organic gas removal system （OGRS）＂Closed/Opened＂ in turn. When the OGRS is closed only with two NP10 getters for purification, the 40At intensities increase in curves with inlet time because of impurities, the 40Ar/39Ar dating results yield age errors about ＋2%-±1% （20-）. When the OGRS is opened for purification, in contrast, the 40Ar intensities decrease linearly with inlet time. This indicates that the gases have been cleaned up effectively, and the 40Ar/39Ar results yield ages with errors in ±0.4%. The OGRS is very helpful to obtain high-quality analysis data.

关键词： 40Ar/39Ar dating Gas purification technique Organic impurity Noble gas

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Histological Study on Reproductive Organ of Rana Temporaria Chensinesis David

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Journal of Northeast Agricultural University(English Edition) 2006年第1期13卷 41-46页

作者： HUANG He TIAN Yaguang NINGFangyong bai xiujuan ZHANG Guixue College of Animal Science and Technology Northeast Agricultural University Harbin Heilongjiang 150030 PRC

The experiment was designed to study the histology of reproductive organ of Rana temporaria chensinesis David in reproductive season and dereproductive season, The results displayed that the sexual gland weight and oviduct weight of the Rana temporaria chensinesis David were significantly different between reproductive season and dereproductive season.

关键词： Rana temporaria chensinesis David histology reproductive organ

四川盆地震旦-寒武系油气成藏的Re-Os年代学约束

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地球科学 2019年第3期44卷 713-726页

作者：沈传波葛翔白秀娟中国地质大学构造与油气资源教育部重点实验室湖北武汉430074 中国地质大学资源学院湖北武汉430074 英国杜伦大学地球科学系

四川盆地震旦-寒武系储层具有万亿立方米以上的油气地质储量,具有广阔的勘探前景.复杂的地质条件及多期次构造作用限制了对其油气成藏演化过程的精细刻画.近年来,流体包裹体^(40)Ar/^(39)Ar和烃类Re-Os同位素定年等新技术在油气成藏研... 详细信息

四川盆地震旦-寒武系储层具有万亿立方米以上的油气地质储量,具有广阔的勘探前景.复杂的地质条件及多期次构造作用限制了对其油气成藏演化过程的精细刻画.近年来,流体包裹体^(40)Ar/^(39)Ar和烃类Re-Os同位素定年等新技术在油气成藏研究中表现出良好的潜力和广泛的应用前景.针对四川盆地深部油气成藏演化的定量解析这一问题,在总结前人对震旦-寒武系油气成藏演化地质分析的基础上,结合近期对川中威远气田、川西龙门山矿山梁古油藏和川北米仓山古油藏中沥青Re-Os同位素的定年结果,认为四川盆地震旦-寒武系油气存在～450Ma、205～162Ma两期成藏作用,其中天然气藏形成的关键时期为205～162Ma.还指出了Re-Os同位素分析在定量解析油气演化研究中需要解决的关键问题,并认为烃类的Re-Os同位素定年将会推动我国成藏年代学的发展,促进诸如四川盆地等复杂地质条件深层油气成藏过程和成藏机理的研究.

关键词：成藏作用古油藏沥青成藏年代 Re-Os定年石油地质