Male heterogametic sex determination in Rana dybowskii based on sex-linked molecular markers

作     者：Yuan XU Zhiheng DU Jiayu LIU Hang SU Fangyong NING Shiquan CUI Lijuan WANG Jianming LIU Chuanshuai REN Shengwei DI Xiujuan BAI 

作者机构：College of Animal Science and TechnologyNortheast Agricultural UniversityHarbinChina College of Life SciencesNortheast Agricultural UniversityHarbinChina College of AgriculturalEastern Liaoning UniversityDandongChina Yili Animal Science Research InstituteYiningChina Animal Husbandry Administration of HuadianHuadianChina Branch of Animal Husbandry and Veterinary of Heilongjiang Academy of Agricultural SciencesQiqiharChina 

出 版 物：《Integrative Zoology》 (整合动物学（英文版）)

年 卷 期：2022年第17卷第1期

页      面：105-114页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 0905[农学-畜牧学] 0906[农学-兽医学] 071002[理学-动物学] 

基　　金：funded by China Postdoctoral Science Foundation(No.2021M690577) the Post-Doctoral Fund of Heilongjiang Province(No.LBH-Z20113) 

主　　题：male-linked molecular markers Rana dybowskii sexual reversal TRAP markers 

摘      要：Identifying the mechanism for sex determination in amphibians is *** little is known about sex determination mechanisms of Rana dybowskii,a species of importance to evolutionary and conservation *** screened for sex-linked molecular markers in *** in China using target region amplification polymorphism with 2 fixed primers against the sequences of *** found 2 male-linked molecular markers in ***,which were 222 bp and 261 bp *** detection rates of 222 bp marker in males form Xinglong,Huadian,and Dandong were 93.79%,69.64%,and 13.64%,respectively,while the rate in females from Huadian was 27.50%.Besides,the detection rates of 261 bp marker in the above 3 regions were only observed in males at the rate of 93.79%,87.50%,and 32.73%,*** inheritance patterns of sex-linked molecular markers showed that the 2 sex-linked molecular markers were *** to the XY-male parent,progeny from XX-pseudo-male parent possessed lower sex reversal ratio at the same rearing temperature,and the proportion of female froglets from an XX-pseudo-male parent was more than 95%at low rearing temperature(15°C).Our findings suggest that *** displays male heterogamety,and the 2 sex-linked molecular markers may have a guiding significance for the protection and utilization of ***.