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Curcumin inhibits proliferation of human lens epithelial cells:a proteomic analysis

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2012年第5期13卷 402-407页

作者： Yan-hong HU xiu-rong huang Ming-xin QI Bu-yuan HOU Department of Ophthalmology the Second Affiliated Hospital of Fujian University of Traditional Chinese MedicineFuzhou 350003China Research Center of Pathophysiology Fujian University of Traditional Chinese MedicineFuzhou 350003China

Objective: The incidence of after-cataracts [also known as posterior capsular opacification (PCO)] is between 30% and 50% three years following cataract surgery. Suppressing the proliferation of lens epithelial cells (LECs) is a primary goal in preventing PCO. Here, we investigated the proteomic regulation of the inhibitory effects of curcumin (Cur) on the proliferation of human lens epithelial B3 (HLE-B3) cells. Methods: Recombinant human basic fibroblast growth factor (rhbFGF) was used to induce proliferation of HLE-B3 cells, which were incubated with 20 mg/L Cur in a CO2 incubator for 24 h. Results: We found that the absorbance (A) value of rhbFGF group was significantly higher than the A value of the control group. Furthermore, the A value of the Cur group was significantly lower com- pared to the rhbFGF group, with an inhibition of 53.7%. Five different protein spots were obtained from proliferative HLE-B3 cells induced by rhbFGF. Eight different protein spots were obtained in HLE-B3 cells incubated with Cur. There were the common variational protein spots at mass/charge (m/z) ratios of 8 093 and 13 767 between rhbFGF group and control group as well as between the Cur group and rhbFGF group. Conclusions: These results show that Cur effectively inhibited HLE-B3 cell proliferation induced by rhbFGF. The protein spots at m/z of 8 093 and 13 767 may be the targets of Cur-induced inhibition of HLE-B3 cell proliferation. Cur may be a reliable and effective drug for pre- vention and treatment of polymerase chain reaction (PCR).

关键词： Curcumin After-cataract Posterior capsular opacification (PCO) Proteomics Lens epithelial cells

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Copper catalyzed borylative cyclization of 3-arylallyl carbamoyl chloride with B_(2)pin_(2):stereoselective synthesis of cis-2-aryl-3-boryl-γ-lactams

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Chinese Chemical Letters 2021年第7期32卷 2297-2300页

作者： Mei-Hua Shen Ting-Biao Wan xiu-rong huang Yan Li Dan-Hua Qian Hua-Dong Xu Defeng Xu Jiangsu Key Laboratory of Advanced Catalytic Materials&Technology School of PharmacyChangzhou UniversityChangzhou 213164China

Borylative cyclization of E-3-arylallyl carbamoyl chlorides is achieved through copper catalyzed intramolecular carboboration with B_(2)pin_(2).2-Aryl-3-boryl-γ-lactams are formed with exclusive ***-Dppp combination ... 详细信息

关键词： Carboboration Borylative cyclization Copper catalysis Allyl carbamoyl chloride Borocupration γ-Lactam

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Different Pretreatments before Application of GnRH Antagonist Protocol in Controlled Ovarian Hyperstimulation

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Journal of Reproduction and Contraception 2011年第4期22卷 201-210页

作者： Ping-xiu huang rong LI Min FU Juan-juan WANG Reproductive Medical Centre Shenzhen Hospital of Peking University Shenzhen 518036 China

Objective To analyze the clinical features and outcome of patients who used different pretreatments before application of gonadotropin-releasing hormone antagonist protocol during in vitro fertilization - embryo transfer （IVF-ET） cycles, and to explore how effective to use the antagonist protocol. Methods A retrospective analysis was performed. All the ET cycles were divided into three groups, group A （n=125） used short acting GnRH agonist before GnRH antagonist treatment, group B （n=113） used short-acting oral contraceptives before GnRH antagonist treatment, group C （n=81） was untreated before GnRH antagonist treatment. All the patients had no tubal fluid, endometrial polyps and no anatomical abnormalities of the uterus, from April 2010 to December 2010. The patient＇s age, dose and duration of gonadotropin （Gn） treatment, the serum LH and E2 levels on the day of hCG injection, the number of oocytes retrieved, the rates of good-quality embryos, the clinical pregnancy rates were compared. At the same time, 261 GnRH agonist long protocol cycles （group D） were selected at the same period as further comparison. Results The patients in group C （32.9 ~ 4.8 years） were significantly older than those in groups A and B （31.6 ___＋3.7 years, 31.2 ___%4.1 years）（P 〈0.05）. The dose and the duration of Gn in group C were significantly lower than those in groups A and B. The serum LH level on the day of hCG injection in group A and group B was significantly lower than that in group C （P 〈0.05）, especially in group A. The endometrium was the thinnest in group B. There were no significant differences in the fertilization rates and the good-quality embryosrates among them. The clinical pregnancy rate of group B decreased significantly compared with groups A and C （P〈0. 05）. The clinical pregnancy rate of group C was the highest among them. There was no significant difference of clinical pregnancy rates between group C and group D （37% vs 40.2%,P〉0.05）. However, the dose （19.8 ±6.6 am

关键词： in vitro fertilization-embryo transfer (IVF-ET) gonadotropin-releasing hormone antagonist short-acting oral contraceptives short-acting agonist

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Mitochondrial proteomic analysis of ecdysterone protection against oxidative damage in human lens epithelial cells

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International Journal of Ophthalmology(English edition) 2014年第1期7卷 38-43页

作者： Chun-Yan Feng xiu-rong huang Ming-Xin Qi Song-Wen Tang Sheng Chen Yan-Hong Hu Fa-Jie Ke Xin Wang Department of Ophthalmology Second Affiliated Peoples Hospital of Fujian University of Traditional Chinese Medicine Research Center of Pathophysiology Fujian University of Traditional Chinese Medicine Department of Laboratory Fujian University of Traditional Chinese Medicine subsidiary Rehabilitation Hospital

AIM: To investigate the protective effects of the natural medicinal monomer ecdysterone(ECR) with estrogenic activity against oxidative damage in human lens epithelial cells B3(HLE-B3) caused by hydrogen peroxide 21(H2 O2) and to pursue the possible mitochondrial proteomic regularity of the protective effects. · METHODS: HLE-B3 cells were treated with H2O2(300μmol/L),β-estuarial(E2; 10-8mol/L) and H2 O2,ECR(10-6mol/L) and H2 O2,or left untreated. Altered expression of all mitochondrial proteins was analyzed by protein array and surface-enhanced laser desorption ionization time of flight mass spectrometry(SELDI-TOF-MS). The mass/charge(M/Z) ratios of each peak were tested by the Kruskal-Wallis rank sum test,and the protein peak value of the M/Z ratio for each treatment by pair comparison was analyzed with the Nemenyi test. ·RESULTS: H2O2 up-regulated expression of two protein spots(with M/Z of 6 532 and 6 809). When E2 mitigated the oxidative damage,the expression of one protein spot(M/Z 6 532) was down-regulated. In contrast,ECR downregulated both of protein spots(M/Z 6 532 and 6 809). · CONCLUSION: ECR could effectively inhibite H2O2 induced oxidative damage in HLE-B3 cells. The protein spot at M/Z of 6 532 might be the target spot of ECR against oxidative damage induced by H2 O2.

关键词： ecdysterone mitochondrial proteomics lens epithelial cell senile cataract

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Effects of Dendrobium candidum polysaccharides on microRNA-125b and mitogen-activated protein kinase signaling pathways in diabetic cataract rats

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Traditional Medicine Research 2021年第5期6卷 43-48页

作者： Zi-Yang Chen Qian Lan Sheng Chen Yan-Hong Hu Fa-Jie Ke Jun Hu Chun-Yan Feng Ming-Xin Qi xiu-rong huang Comprehensive Clinic of Fujian Academy of Chinese Medicine Fuzhou 350003China Fujian University of Traditional Chinese Medicine Fuzhou 350122China Department of Opthalmology The Second Affiliated Hospital of Fujian Traditional Chinese Medical UniversityFuzhou 350003China

Background:Diabetic cataract is a common complication and a lens disorder in ***,Dendrobium officinale polysaccharide(DOP)is found to alleviate diabetes ***,microRNA-125b and mitogen-activated protein kinase signaling pathways play significant roles in ***,the mechanism and the effect of DOP on diabetic cataract remains ***:Wistar male rats were randomly assigned to the control,model,and DOP *** was induced with ***,DOP was orally given for 12 *** Lens Opacities Classification System III was used to evaluate lens opacity by slit-lamp *** lens was then harvested for testing the mRNA expression of microRNA-125b,ERK1,ERK2,Raf and Ras using reverse transcription-polymerase chain *** protein expression of ERK1,ERK2,Raf,and Ras was detected using the Western blot *** targets of microRNA-125b were predicted in miRWalk *** targets were obtained from the Kyoto Encyclopedia of Genes and Genomes ***:The lens opacities of the rats in the control group were almost at ***,the majority of the lens opacities in the model group were C3 to C4 and N1 to N2,and were mostly at C1 to C2 and N0 to N1 in the DOP *** difference was statistically significant(P<0.05).Furthermore,the microRNA-125b expression in the model group is significantly higher compared with the control ***,the microRNA-125b expression in the DOP group is significantly decreased(all P<0.05).The mRNA expression of ERK1,ERK2,Raf,and Ras in the model groups were upregulated compared with those of the control ***,the ERK1 and Raf mRNA expressions of the DOP group were lower compared with the model group(all P<0.05).The protein expression of ERK1,Raf,and Ras in the model group was significantly increased compared with those of the control *** protein expression of ERK1,Raf,and Ras in the DOP group was significantly lower compared with

关键词： Dendrobium officinale polysaccharide microRNA-125b mitogen-activated protein kinase diabetic cataract

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PD-YOLO:Colon Polyp Detection Model Based on Enhanced Small-Target Feature Extraction

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Computers, Materials & Continua 2025年第1期82卷 913-928页

作者： Yicong Yu Kaixin Lin Jiajun Hong rong-Guei Tsai Yuanzhi huang New Engineering Industry College Putian UniversityPutian351100China Engineering Research Center of Big Data Application in Private Health Medicine(Putian University) Fujian Province UniversityPutian351100China Computer College Guangdong University of Science&TechnologyDongguan523668China

In recent years,the number of patientswith colon disease has increased *** polyps are the precursor lesions of colon *** not diagnosed in time,they can easily develop into colon cancer,posing a serious threat to patients’lives and health.A colonoscopy is an important means of detecting colon ***,in polyp imaging,due to the large differences and diverse types of polyps in size,shape,color,etc.,traditional detection methods face the problem of high false positive rates,which creates problems for doctors during the diagnosis *** order to improve the accuracy and efficiency of colon polyp detection,this question proposes a network model suitable for colon polyp detection(PD-YOLO).This method introduces the self-attention mechanism CBAM(Convolutional Block Attention Module)in the backbone layer based on YOLOv7,allowing themodel to adaptively focus on key information and ignore the unimportant *** help themodel do a better job of polyp localization and bounding box regression,add the SPD-Conv(Symmetric Positive Definite Convolution)module to the neck layer and use deconvolution instead of *** results indicate that the PD-YOLO algorithm demonstrates strong robustness in colon polyp *** to the original YOLOv7,on the Kvasir-SEG dataset,PD-YOLO has shown an increase of 5.44 percentage points in AP@0.5,showcasing significant advantages over other mainstream methods.

关键词： Polyp detection YOLOv7 SPD-Conv CBAM deconvolution

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在鼻泪管阻塞性疾病中应用CT测量鼻泪道相关解剖参数的研究进展

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国际眼科杂志 2020年第4期20卷 646-650页

作者：刘欣莫亚黄秀蓉成都中医药大学四川成都610075 成都中医药大学附属医院眼科四川成都610075

鼻泪管阻塞性疾病(NLDOD)是临床发病率较高的一种眼科常见病,是由于炎症或其他原因引起的鼻泪管部分或全部阻塞不通,主要表现为溢泪,继发泪囊炎可出现溢脓,继发泪囊囊肿导致泪囊扩大、内眦肿胀等,开通阻塞的鼻泪管则是治疗鼻泪管阻塞性... 详细信息

鼻泪管阻塞性疾病(NLDOD)是临床发病率较高的一种眼科常见病,是由于炎症或其他原因引起的鼻泪管部分或全部阻塞不通,主要表现为溢泪,继发泪囊炎可出现溢脓,继发泪囊囊肿导致泪囊扩大、内眦肿胀等,开通阻塞的鼻泪管则是治疗鼻泪管阻塞性疾病的关键,明确鼻泪道相关解剖参数在鼻泪管阻塞性疾病的诊断和治疗中显得极为重要。而泪道CT是泪道影像学检查常用方法之一,便于对鼻泪道相关解剖参数进行测量。本文综述近年来在鼻泪管阻塞性疾病中应用CT测量鼻泪道相关解剖参数的研究进展,主要包括鼻泪道及泪囊的经线、面积、体积、角度等,分析总结相关解剖参数与鼻泪管阻塞性疾病的相关性,以便更好地指导临床。

关键词：鼻泪管阻塞性疾病鼻泪管泪囊解剖参数

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高度近视合并白内障的超声乳化手术治疗进展

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国际眼科杂志 2010年第3期10卷 504-505页

作者：闫亚红黄秀蓉成都中医药大学中国四川省成都市610075 成都中医药大学附属医院眼科中国四川省成都市610075

我们通过对高度近视合并白内障的分析及相关手术治疗的研究，对该病的超声乳化手术治疗进展进行了总结。

关键词：高度近视白内障超声乳化手术治疗

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Curcumin inhibits proliferation of human lens epithelial cells： a proteomic analysis

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2013年第2期14卷 86-86页

作者： Yan-hong HU xiu-rong huang Ming-xin QI Bu-yuan HOU

Objective： The incidence of after-cataracts [also known as posterior capsular opacification （PCO）] is between 30% and 50% three years following cataract surgery. Suppressing the proliferation of lens epithelial cells （LECs） is a primary goal in preventing PCO. Here, we investigated the proteomic regulation of the in- hibitory effects of curcumin （Cur） on the proliferation of human lens epithelial B3 （HLE-B3） cells. Methods： Recombinant human basic fibroblast growth factor （rhbFGF） was used to induce proliferation of HLE-B3 cells, which were incubated with 20 mg/L Cur in a CO2 incubator for 24 h. Results： We found that the absorbance （,4） value ofrhbFGF group was significantly higher than the A value of the control group. Furthermore, the A value of the Cur group was significantly lower compared to the rhbFGF group, with an inhibition of 53.7%. Five different protein spots were obtained from proliferative HLE-B3 cells induced by rhbFGF, Eight different protein spots were obtained in HLE-B3 cells incubated with Cur. There were the common variational protein spots at mass/charge （m/z） ratios of 8 093 and 13 767 between rhbFGF group and control group as well as between the Cur group and rhbFGF group. Conclusions： These results show that Cur effectively inhibited HLE-B3 cell proliferation induced by rhbFGF. The protein spots at m/z of 8 093 and 13 767 may be the targets of Cur-induced inhibition of riLE-B3 cell proliferation. Cur may be a reliable and effective drug for prevention and treatment of polymerase chain reaction （PCR）.

关键词：晶状体上皮细胞蛋白质组分细胞增殖姜黄素重组人碱性成纤维细胞生长因子白内障手术 CO2培养箱聚合酶链反应

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Curcumin inhibits proliferation of human lens epithelial cells:a proteomic analysis

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2012年第12期13卷 1005-1005页

作者： Yan-hong HU,xiu-rong huang,Ming-xin QI,Bu-yuan HOU

Objective:The incidence of after-cataracts [also known as posterior capsular opacification (PCO)] is between 30% and 50% three years following cataract *** the proliferation of lens epithelial cells (LECs) is a primary goal in preventing ***,we investigated the proteomic regulation of the inhibitory effects of curcumin (Cur) on the proliferation of human lens epithelial B3 (HLE-B3) ***:Recombinant human basic fibroblast growth factor (rhbFGF) was used to induce proliferation of HLE-B3 cells,which were incubated with 20 mg/L Cur in a CO 2 incubator for 24 ***:We found that the absorbance (A) value of rhbFGF group was significantly higher than the A value of the control ***,the A value of the Cur group was significantly lower compared to the rhbFGF group,with an inhibition of 53.7%.Five different protein spots were obtained from proliferative HLE-B3 cells induced by *** different protein spots were obtained in HLE-B3 cells incubated with *** were the common variational protein spots at mass/charge (m/z) ratios of 8093 and 13767 between rhbFGF group and control group as well as between the Cur group and rhbFGF ***:These results show that Cur effectively inhibited HLE-B3 cell proliferation induced by *** protein spots at m/z of 8093 and 13767 may be the targets of Cur-induced inhibition of HLE-B3 cell *** may be a reliable and effective drug for prevention and treatment of polymerase chain reaction (PCR).

关键词：晶状体上皮细胞蛋白质组分细胞增殖姜黄素重组人碱性成纤维细胞生长因子白内障手术 CO2培养箱聚合酶链反应

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