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MicroRNA-15a/b are up-regulated in response to myocardial ischemia/reperfusion injury

Journal of Geriatric Cardiology 2012年第1期9卷 28-32页

作者： Li-Feng Liu Zhuo Liang Zhen-Rong Lv xiu-hua Liu Jing Bai Jie Chen Chen Chen Yu Wang Institute of Geriatric Cardiology Chinese PLA General Hospital 28 Fuxing Road Beijing 100853 China Department of Cardiology Xijing Hospital the Fourth Military Medical University 17 Changle Road Xi'an 710032 Shaanxi China Cardiovascular Research Institute and Department of Cardiology Shenyang Northern Hospital 83 Wenhua Road Shenyang 110016 Liaoning China Department of Pathophysiology Shandong University School of Medicine Jinan 250012 Shandong China Department of Pathophysiology Chinese PLA General Hospital 28 Fuxing Road Beijing 100853 China

Objective Several studies have indicated that miR-15a,miR-15b and miR-16 may be the important regulators of *** attenuate apoptosis could protect myocardium and reduce infarction size,the present study was aimed to find out whether these miRNAs participate in regulating myocardial ischemia reperfusion （I/R） *** Apoptosis in mice hearts subjected to I/R was detected by TUNEL assay in vivo,while flow cytometry analysis followed by Annexin V/PI double stain in vitro was used to detect apoptosis in cultured cardiomyocytes which were subjected to hypoxia/reoxygenation （H/R）.Taqman real-time quantitative PCR was used to confirm whether miR-15a/15b/16 were involved in the regulation of cardiac I/R and H/*** Compared to those of the controls,I/R or H/R induced apoptosis of cardiomyocytes was significantly iucreased both in vivo （24.4％ ± 9.4％ vs.2.2％ ± 1.9％,P ＜ 0.01,n =5） and in vitro （14.12％ ±0.92％ vs.2.22％ ± 0.08％）.The expression of miR-15a and miR-15b,but not miR-16,was increased in the mice I/R model,and the results were consistent in the H/R *** Our data indicate miR-15 and miR-15b are up-regulated in response to cardiac I/R injury,therefore,down-regulation of miR- 15a/b may be a promising strategy to reduce myocardial apoptosis induced by cardiac I/R injury.

关键词： miR-15a/b Apoptosis Myocardial reperfusion injury Ischemia/Reperfusion injury

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Simultaneous Determination of Five Components Including Acetaminophen by Reversed-phase High Performance Liquid Chromatography

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Journal of Wuhan University of Technology(Materials Science) 2004年第1期19卷 34-35页

作者：张立庆 Zhejiang University of Science and Technology

High performance liquid chromatography with a C18 reverse-phase column was used to separate the five components in cough syrup,including acetaminophen,p-aminophenol,caffeine,chlorphenamine maleate and *** mobile phase consists of 15wt% acetonitrile,0.004mol/L sodium heptyl sulfonate, 0.03mole/L potassium di-hydrogen phosphate and triethylamine (volume ratio 13∶40∶44∶3),the pH of which is adjusted to 3.0 by phosphoric *** contents of the five components are analyzed on an ultraviolet spectrophotometer at 254nm,with a flow rate of 0.4mL/*** results show that the calibration curves are linear in a certain *** average recovery of five components is between 96.31% and 102.3%.

关键词： organic compounds preparation and determination chromatography

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Effects of dietary supplementation with vitamin E and selenium on rat hepatic stellate cell apoptosis

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World Journal of Gastroenterology 2005年第32期11卷 4957-4961页

作者： xiu-hua Shen Wu-Feng Cheng Xuan-Hai Li Jian-Qin Sun Feng Li Ling Ma Liang-Min Xie Department of Nutrition Shanghai Second Medical University Shanghai 200025 China epartment of Nutrition Huadong Hospital Shanghai 200040 China Department of Nutrition Shanghai East Hospital Shanghai 200120 China Department of Nutrition Shanghai East Hospital Shanghai 200120 China

AIM： To evaluate the effects of dietary supplementation with vitamin E and selenium on proliferation and apoptosis of hepatic stellate cells （HSCs）, in acute liver injury induced by CCI4, and to explore their role in the recovery from hepatic fibrosis phase. METHODS： An acute liver damage model of rats was established by intraperitoneal injection of carbon tetrachloride （0.3 ml/100 g body weight） twice a week, then the rats were killed at 6, 24, 48, and 72 h after the first and third injection, respectively. A liver fibrosis model was established by the same injection for 8 wk. Then three rats were killed at 3, 7, 14, and 28 d after the last injection, respectively. The rats from the intervention group were fed with chow supplemented with vitamin E （250 mg/kg） and selenium （0.2 mg/kg）, and the rats in the normal control group and pathological group were given standard chow. Livers were harvested and stained with hematoxylin and eosin, Sirius red. Activated HSCs were determined by s-smooth muscle actin immunohistochemistry staining. Apoptotic HSCs were determined by dual staining with the terminal deoxynucleotidyl transferase UTP nick end labeling （TUNEL） and α-smooth muscle actin immunohistochemistry. Serum alanine aminotransferase and aspartate aminotransferase were also analyzed. RESULTS： In the acute liver damage model, the degree of liver injury was more serious in the pathological group than in the intervention group. At each time point, the number of activated HSCs was less in the intervention group than in the pathological group, while the number of apoptotic HSCs was more in the intervention group than in the pathological group. In the liver fibrosis model, the degree of liver fibrosis was more serious in the pathological group than in the intervention group. At each time point, the number of activated HSCs was less in the intervention group than in the pathological group, and the number of apoptotic HSCs was more in the intervention group than in the pathol

关键词： Vitamin E Selenium Hepatic stellate cell Apoptosis

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Oleuropein Improves Long Term Potentiation at Perforant Path-dentate Gyrus Synapses in vivo

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Chinese Herbal Medicines 2015年第3期7卷 255-260页

作者： Fei-fei Pu Song Yin Hong-ying Chen Zhi Dai Tian-xiu Qian Cheng-cheng Wang Hui Xu Xiao-ying Wang Institute of Medicinal Plant Development Chinese Academy of Medical Sciences and Peking Union Medical College Department of Pharmacy Maternal and Child Hospital of Xiangtan China Resources Sanjiu Medical & Pharmaceutical Co. Ltd.

Objective To investigate the effect of oleuropein （OE） on long term potentiation （LTP） at hippocampal perforant path-dentate gyrus synapses in vivo. Methods An outer guide cannula, a monopolar recording electrode, and a bipolar stimulating electrode were implanted in the skull and extracellular recording technique was used to record the population spike in the dentate gyrus of anesthetized rats. Results Oleuropein significantly increased the basal synaptic transmission and the amplitude of population spike was increased from （11 7.6 ＋ 2.3）% to （134.9 _＋ 3.7）% after administration with OE. OE also accelerated LTP induction and maintenance, the population spike amplitude after high frequency stimulation was increased from （167.2 ± 12.8）% to （225.5 ± 15.5）% and the maintenance phase of LTP was from （182.1 ± 15.1）% to （210.5 ± 9.0）% respectively after administration with OE. Conclusion Present study showed that OE significantly improved different stages of LTP, which could be the molecular mechanism of its efficacy on attenuating AD-like pathology and delaying cognitive decline. OE can be a promising drug for AD and dementia.

关键词： Alzheimer's disease dentate gyrus long term potentiation oleuropein population spike

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基于机器学习的青藏高原高寒沼泽湿地蒸散发插补研究

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植物生态学报 2023年第7期47卷 912-921页

作者：王秀英陈奇杜华礼张睿马红璐青海省气象科学研究所青海省防灾减灾重点实验室西宁810001

以青藏高原典型高寒沼泽湿地为观测研究站,以实际蒸散发为研究对象,结合气象因子(净辐射、气温、土壤热通量、风速、相对湿度、土壤含水率),建立基于多元线性回归(MLR)、决策树(CART)、随机森林(RF)、支持向量回归(SVR)、多层感知机(ML... 详细信息

以青藏高原典型高寒沼泽湿地为观测研究站,以实际蒸散发为研究对象,结合气象因子(净辐射、气温、土壤热通量、风速、相对湿度、土壤含水率),建立基于多元线性回归(MLR)、决策树(CART)、随机森林(RF)、支持向量回归(SVR)、多层感知机(MLP)7种组合5类算法的预测模型,找出对于蒸散发具有较高精度的插补方法,实现实际蒸散发数据集的构建。结果表明:1)研究区蒸散发与净辐射相关性最大,而土壤热通量是影响蒸散发过程的关键因子;2)7种组合的5类机器学习算法模型的决定系数变化范围为0.58-0.83,均方根误差变化范围为0.038-0.089 mm·30 min^(-1);2)随机森林回归模型决定系数最高,模型稳定性最佳,插补效果最优;3)插补完整的蒸散发与净辐射、土壤热通量、气温日尺度变化趋势相同,与风速、相对湿度变化趋势相反。日蒸散发主要集中在生长季,日最大值为8.77 mm·d^(-1),出现在7月9日,日最小值为0.21 mm·d^(-1),出现在1月30日。

关键词：机器学习高寒沼泽湿地蒸散发交叉验证

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Levels and dynamic changes of serum fibroblast growth factor 23 in hypophosphatemic rickets/osteomalacia

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Chinese Medical Journal 2010年第9期123卷 1158-1162页

作者： XIA Wei-bo JIANG Yan LI Mei XING Xiao-ping WANG Ou HU ying-ying ZHANG hua-bing LIU huai-cheng MENG Xun-wu ZHOU Xue-ying Department of Endocrinology Key Laboratory of EndocrinologyMinistry of HealthPeking Union Medical College HospitalChinese Academy of Medical SciencesBeijing 100730China

Background Hypophosphatemic rickets/osteomalacia is a group of diseases characterised by defective mineralization of bone due to hypophosphatemia and low 1,25-dihydroxy vitamin D. To explore the role of fibroblast growth factor 23 （FGF-23） in the regulation of phosphate homeostasis, we measured the circulating concentrations of this growth factor in healthy individuals and in patients with hypophosphatemic rickets/osteomalacia. Methods Nineteen patients with hypophosphatemic rickets/osteomalacia were included in hypophosphatemic group （HP, 12 female and 7 male, mean age was 30 years）, and 19 healthy age-matched individuals served as the control group. Full length FGF-23 fragments were measured by two-site enzyme-linked immunosorbent *** Mean FGF-23 concentrations were significantly higher in the HP group （（87.4±43.6） pg/ml） compared with the control group （（19.2±6.16） pg/ml; P 〈0.001）. In 1 patient with tumour-induced osteomalacia, serum FGF-23 concentrations were 84.1 pg/ml; these concentrations were normalized 2 hours after a hemangiopericytoma resection （7.8 pg/ml）. Subsequently, serum 1,25（OH）2 vitamin D3 concentrations significantly increased from 21.3 pg/ml to 89.3 pg/ml, and serum phosphorus levels were normalized. Conclusions Serum FGF-23 concentrations were markedly elevated in patients with hypophosphatemic rickets. FGF-23 plays an important role in the pathogenesis of hypophosphatemic rickets/osteomalacia.

关键词： fibroblast growth factor 23 hypophosphatemia rickets osteomalacia

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H9亚型禽流感病毒M1蛋白原核表达及免疫效果初步研究

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动物医学进展 2024年第6期45卷 1-6页

作者：李小凤谢芝勋阮志华李孟李丹张民秀谢志勤罗思思韦悠谢丽基曾婷婷张艳芳黄娇玲王盛广西壮族自治区兽医研究所/广西兽医生物技术重点实验室/农业农村部中国(广西)-东盟跨境动物疫病防控重点实验室广西南宁530001

旨在原核表达H9亚型禽流感病毒(AIV)M1蛋白,初步探究其免疫效果,为研制AIV疫苗奠定基础。通过构建重组质粒pET-32a-M1,诱导表达,Western blot验证蛋白表达情况,将重组蛋白免疫接种小鼠,通过测定血清中IgG抗体效价,脾细胞培养上清多细胞... 详细信息

旨在原核表达H9亚型禽流感病毒(AIV)M1蛋白,初步探究其免疫效果,为研制AIV疫苗奠定基础。通过构建重组质粒pET-32a-M1,诱导表达,Western blot验证蛋白表达情况,将重组蛋白免疫接种小鼠,通过测定血清中IgG抗体效价,脾细胞培养上清多细胞因子分泌情况评价其免疫效果。结果显示,M1重组蛋白主要以可溶性蛋白存在,Western blot结果可见特异性条带。免疫接种小鼠后,血清产生IgG抗体,抗体效价为1∶30000。小鼠脾细胞上清液IL-2、IL-4、IL-6与对照组相比较,含量显著增加(P<0.01),而抑制IL-1β、IL-5、IL-13、IL-12p70、IL-18、GM-CMF、IFN-γ、TNF-α分泌。结果表明,M1重组蛋白免疫原性良好,刺激机体产生体液免疫和细胞免疫,为禽流感疫苗深入研究奠定了基础。

关键词：禽流感 M1蛋白原核表达免疫效果

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Therapeutic Observation of Warm Needling plus Tuina for Senile Functional Constipation

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Journal of Acupuncture and Tuina Science 2014年第5期12卷 295-299页

作者： Li ying hua Yu Zhong Ning Hong Jue Community Health Service Center Huajing Township Xuhui DistrictShanghai

Objective: To observe the clinical efficacy of warm needling plus tuina in treating senile functional constipation. Methods: Ninety elderly patients with functional constipation were randomized into two groups. Forty-six patients in the treatment group were intervened by warm needling plus tuina; the other forty-four patients in the control group were intervened by San huang tablet. Results: In the treatment group, 19 patients were cured, 24 cases showed improvement, 3 cases failed, and the total effective rate was 93.5%; in the control group, 16 patients were cured, 18 cases showed improvement, 10 cases failed, and the total effective rate was 77.3%. There was a significant difference in comparing the therapeutic efficacy(Phuang tablet in treating senile functional constipation.

关键词： Acupuncture Therapy Tuina Massage Warm Needling Therapy Moxibustion Therapy Constipation Aged

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肝移植术后肺结核患者免疫状态与主要器官功能的初步分析

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国际医药卫生导报 2024年第16期30卷 2641-2645页

作者：傅红梅俞朝贤王娟李春燕谭毅刚岑滢李华刘志辉广州市胸科医院结核内科广州510095 广州市胸科医院重症医学科广州510095 广州市胸科医院肺部疾病研究所广州510095 广州市胸科医院医学检验科广州510095

目的初步探讨肝移植术后肺结核患者免疫状态与器官功能相较于普通肺结核患者的临床特点,为规范抗结核临床诊疗提供科学依据。方法回顾性收集2014年至2023年期间广州市胸科医院住院患者的资料,以24例肝移植术后肺结核住院患者作为研究组... 详细信息

目的初步探讨肝移植术后肺结核患者免疫状态与器官功能相较于普通肺结核患者的临床特点,为规范抗结核临床诊疗提供科学依据。方法回顾性收集2014年至2023年期间广州市胸科医院住院患者的资料,以24例肝移植术后肺结核住院患者作为研究组,31例同期非器官移植肺结核住院患者作为对照组。研究组均为男性;年龄37~75岁,平均年龄57岁。对照组均为男性;年龄32~75岁,平均年龄57岁。收集两组就诊后首次血常规、炎症标志物、肝功能、肾功能、心功能、T淋巴细胞亚群等实验室检测指标结果。比较两组上述指标的异常比例、各类白细胞及血小板计数的比率。采用χ^(2)检验和秩和检验。结果研究组白细胞、淋巴细胞、血小板、血红蛋白、γ-谷氨酰转肽酶、肌酐、胱抑素C、肌酸激酶、B型钠尿肽前体以及CD3^(+)、CD4^(+)、CD8^(+)T淋巴细胞亚群检测结果异常率均高于对照组(均P<0.05)。研究组的淋巴细胞-白细胞比率[0.13(0.09,0.17)]、淋巴细胞-中性粒细胞比率[0.17(0.12,0.25)]均低于对照组[0.18(0.12,0.21)、0.26(0.15,0.33)],且单核细胞-淋巴细胞比率[0.59(0.49,0.96)]高于对照组[0.40(0.33,0.63)](均P<0.05)。结论肝移植术后肺结核患者肝、肾、心等主要器官功能受损以及免疫功能低下较多,在临床诊疗过程中须予以高度关注。

关键词：肝移植术结核/肺器官功能免疫功能

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聚苯乙烯二醋酸碘苯在萘普生合成中的应用

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化学世界 2006年第7期47卷 420-422页

作者：陈文华祁秀秀常州工程职业技术学院应化系江苏常州213004

2-萘甲醚(1)在无水AlCl3催化下与丙酰氯反应得6-甲氧基-2-丙酰萘(2),(2)在原甲酸三乙酯、硫酸下采用聚苯乙烯二醋酸碘苯催化进行1,2-芳基迁移重排得2-(2-(6-甲氧基萘基))丙酸乙酯(3),(3)在氢氧化钠水溶液中水解、酸化得萘普生(4)。总收... 详细信息

2-萘甲醚(1)在无水AlCl3催化下与丙酰氯反应得6-甲氧基-2-丙酰萘(2),(2)在原甲酸三乙酯、硫酸下采用聚苯乙烯二醋酸碘苯催化进行1,2-芳基迁移重排得2-(2-(6-甲氧基萘基))丙酸乙酯(3),(3)在氢氧化钠水溶液中水解、酸化得萘普生(4)。总收率约47.4%(以2-萘甲醚计)。试验研究了影响合成2-(2-(6-甲氧基萘基))丙酸乙酯(3)的多种因素,如:硫酸的用量、原甲酸三酯的用量以及聚苯乙烯碘苯的回收利用。

关键词： 2-萘甲醚聚苯乙烯二醋酸碘苯萘普生重排

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