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A biomimetic gradient porous cage with a micro-structure for enhancing mechanical properties and accelerating osseointegration in spinal fusion

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Bioactive Materials 2023年第5期23卷 234-246页

作者： Cheng-Qi Jia Zhen Zhang Shi-Qi Cao Tian-Jiao Wang Hai-Chao Yu Wen-Xiang Wang Bo-Min Guo xiong-ying qiu Yong-Gang You Fan-Qi Hu Jun Zhao Xue-Song Zhang Medical School of Chinese PLA Beijing100853China Department of Orthopedics Chinese PLA General HospitalBeijing100853China Department of Orthopedics of TCM Clinical Unit 6th Medical CenterChinese PLA General HospitalBeijing100853China Research Institute of Aerospace Special Materials and Processing Technology Beijing100074China CAS Key Laboratory of Nanosystem and Hierarchical Fabrication CAS Center for Excellence in NanoscienceNational Center for Nanoscience and TechnologyBeijing100190China Academy of Military Medical Sciences Academy of Military SciencesBeijing100080China University of Chinese Academy of Sciences Beijing100049China

Objectives:Spinal fusion is a widely employed treatment of patients with degenerative disc disease,in which a cage is used to replace the disc for spinal *** it often fails for insufficient mechanical strength and poor ***,we designed a polyether-ether-ketone(PEEK)/tantalum(Ta)composite cage with a biomimetic gradient porous micro-structure,simultaneously enhancing mechanical properties and accelerating osseointegration in spinal *** and methods:In the study,based on the mechanical performances of PEEK and osteogenic potential of Ta,and the three-dimensional(3D)structures of cuttlebone and vertebra,the cages were respectively 3D printed by pure PEEK,PEEK with 5 wt%Ta(PEEK/Ta-5),PEEK with 10 wt%Ta(PEEK/Ta-10)and PEEK with 15 wt%Ta(PEEK/Ta-15),then verifiedin vitroand in sheep cervical fusion model ***:Vertebral Gyroid structure PEEK/Ta-15 cage exhibited superior mechanical properties than Cuttlebone-like structure PEEK/Ta-15 cage,closer to the cervical ***,PEEK/Ta-15 cage with higher Ta microparticles in PEEK provided a biomimetic gradient porous micro-structure with higher surface energy,guiding cell biological behavior,promoting new bone penetration,and accelerating osseointegrationin vivo.Conclusion:In conclusion,the study designed a biomimetic gradient porous cage with a micro-structure for enhancing mechanical properties,accelerating osseointegration and forming an anatomical lock in the fusion segment through composites,mechanical efficiency,surface extension,and pores.

关键词： Biomimetic Porous Composite Osseointegration Spinal fusion

Construction of a small peptide library related to inhibitor OM99-2 and its structure-activity relationship to β-secretase

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Acta Pharmacologica Sinica 2006年第12期27卷 1586-1593页

作者： Bin HU Bing xiong Bei-ying qiu Xin LI Hai-ping YU Kun XIAO Xin WANG Jia LI Jing-kang SHEN~2 State Key Laboratory of Drug Research and National Center for Drug Screening,Shanghai Institute of Materia Medica,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 201203,China State Key Laboratory of Drug Research and National Center for Drug Screening Shanghai Institute of Materia Medica Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China

Aim:To develop probes for detecting the binding specificity between β-secretaseand substrate,and provide reliable biological activity data for further researchingencircling substrate-based ***:To prepare the inhibitors,thehydroxyethylene(HE)segment including P1and P1′ was synthesized after multi-step reactions;the combination of all segments was then completed through solidphase *** human β-secretase ectodomain(amino acid resi-dues 1-460)was expressed as a secreted protein with a C-terminal His tag in insectcells using baculovirus infection,and all compounds were evaluated in thisβ-secretase enzyme *** order to understand the interaction in detail,the theoretical methods,namely molecular dynamics(MD)simulation and mo-lecular mechanics-generalized-born surface area(MM-GBSA)analysis,were per-formed on the complex of β-secretase and OM99-2 to obtain the geometrical andenergetical ***:We designed and constructed a positional scan-ning combinatorial library including 16 compounds;all members of the librarywere synthesized based on HE dipeptide ***-activity relationshipstudies at the P4-P1and P1′-P4′ positions led to the discoveries of P and P’sidesbinding specificity and potent inhibitors 14,18,and *** binding free energyon the whole system and every residue were compared to the biological ***:The removal of P4′ yielded inhibitor 22(A3*B2)with highpotency;further truncation of P3′ gave inhibitor 18(A3*B1)with equal activity,implying that the right side of the inhibitors play a less important role and could beeasily simplified,while change on the P side may cause substantial results.

关键词： β-secretase hydroxyethylene OM99-2 Alzheimer’s disease structure-activity relationship

血管内皮细胞间连接蛋白VE-cadherin和β-连环蛋白在颅内动脉瘤中的表达

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中华实验外科杂志 2011年第2期28卷 255-257页

作者：李美华凌峻熊秋迎聂艳良南昌大学第一附属医院神经外科 330006 南昌大学第一附属医院病理科 330006

目的观察血管内皮钙黏蛋白（VE-cadherin）和β-连环蛋白（β-catenin）在SD大鼠和人颅内动脉瘤壁中的表达,探讨血管内皮细胞连接受损与颅内动脉瘤形成的关系.方法建立肾性高血压诱导的SD大鼠颅内动脉瘤模型,获取8个SD大鼠颅内动脉瘤... 详细信息

目的观察血管内皮钙黏蛋白（VE-cadherin）和β-连环蛋白（β-catenin）在SD大鼠和人颅内动脉瘤壁中的表达,探讨血管内皮细胞连接受损与颅内动脉瘤形成的关系.方法建立肾性高血压诱导的SD大鼠颅内动脉瘤模型,获取8个SD大鼠颅内动脉瘤.另取15例颅内动脉瘤组织标本,应用免疫组织化学方法检测VE-cadherin在大鼠脑动脉瘤壁中的表达,以及VE-cadherin和β-catenin在人脑动脉瘤壁的表达.按照Martina的方法对血管内皮层免疫反应强度进行评分.结果正常SD大鼠和人的脑动脉血管壁上内皮层完整,VE-cadherin呈棕褐色线样均匀表达.SD大鼠颅内动脉瘤壁上VE-cadherin表达减少,呈间断性非均匀分布.正常SD大鼠脑动脉血管壁和颅内动脉瘤壁免疫反应强度评分分别为2.40 ±0.55和1.38±0.51,两组间差异有统计学意义（t=3.41,P＜0.01）.人颅内动脉瘤壁内皮排列紊乱,VE-cadherin和β-catenin表达也明显减少,分布不均匀或表达缺失.正常脑动脉与颅内动脉瘤VE-cadherin和β-catenin免疫反应强度评分分别为2.60±0.54/1.33±0.49和2.80±0.45/1.33±0.49,VE-cadherin和β-catenin在两者间差异均有统计学意义（t分别为4.88和5.91;P＜0.01）.结论颅内动脉瘤的形成过程中存在内皮细胞连接受损.

关键词：颅内动脉瘤 VE—cadherin β-catenin

Regulating the oxygen-atom configuration of carbon anode enabling extremely fast-charging potassium-ion hybrid capacitors

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Nano Research 2025年第1期18卷 373-381页

作者： Daping qiu Yuehui Wang Lulu Zhang Hang Lei Haizhou ying Jin Niu Min Li Xuelin Yang Feng Wang Ru Yang Hubei Provincial Collaborative Innovation Center for New Energy Microgrid College of Electrical Engineering and New EnergyChina Three Gorges UniversityYichang 443002China State Key Laboratory of Chemical Resource Engineering Laboratory of Electrochemical Process and Technology for MaterialsBeijing University of Chemical TechnologyBeijing 100029China College of Materials and Chemical Engineering China Three Gorges UniversityYichang 443002China

Metal-ion hybrid capacitors,such as potassium-ion hybrid capacitors(PIHCs),are regarded as promising fast-charging energy storage ***,the kinetics mismatch between the battery anode and the capacitive cathode restricts their fastcharging *** constructing carbon anodes with enhanced kinetics is an innovative approach to address this ***,using epigallocatechin gallate with high oxygen content as the precursor,oxygen-enriched carbon materials(OEC)with tunable C=O content are successfully ***,the C=O content of OEC is regulated by adjusting the pyrolysis *** as an anode for PIHCs,OEC-600 with the highest C=O content exhibits an attractive fast-charging specific capacity of 135.2 mAh·g^(-1)at 20 A·g^(-1),along with a superior fast-charging cycling *** theoretical calculations,comprehensive kinetics analysis and in-situ Raman,the positive effects of C=O on the potassium storage capability and reversibility of OEC-600 are ***,PIHCs assembled based on an OEC-600 anode deliver impressive energy/power density of 145.1 Wh·kg^(-1)/45.9 kW·kg^(-1)and superior fast-charging cycling stability with 87.5%of capacity retention over 20,000 cycles at 5 A·g^(-1).This work is anticipated to provide an optional design concept toward the carbon anode for fast-charging PIHCs.

关键词： carbon anode oxygen epigallocatechin gallate potassium-ion hybrid capacitor

氯化镧对子宫颈癌HeLa细胞增殖和迁移能力的影响

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中华妇产科杂志 2010年第8期45卷 609-613页

作者：刘丝荪陆丹缪丽芳熊秋迎陈新萍汪泱郭菲南昌大学第一附属医院妇产科 330006 南昌大学第一附属医院研究生院南昌大学第一附属医院病理科南昌大学第一附属医院泌尿外科研究所南昌大学第一附属医院烧伤研究所

目的探讨氯化镧对宫颈癌HeLa细胞增殖和迁移能力的影响，为寻找新的有效治疗宫颈癌的药物提供实验依据。方法宫颈癌细胞株HeLa细胞经培养、传代后分为两组，即实验组（加入5、50、100μmol／L的氯化镧）和对照组（未加入氯化镧）。倒置... 详细信息

目的探讨氯化镧对宫颈癌HeLa细胞增殖和迁移能力的影响，为寻找新的有效治疗宫颈癌的药物提供实验依据。方法宫颈癌细胞株HeLa细胞经培养、传代后分为两组，即实验组（加入5、50、100μmol／L的氯化镧）和对照组（未加入氯化镧）。倒置显微镜下观察两组细胞的生长情况，激光共聚焦显微镜观察两组细胞核的形态变化。采用四甲基偶氮唑蓝（MTT）比色法检测两组细胞的增殖情况，双染色流式细胞仪检测两组细胞的凋亡率，体外迁移实验检测两组细胞迁移能力的变化，逆转录（RT）-PCR技术检测两组细胞中增殖、抗凋亡和迁移相关基因细胞周期蛋白（cyclinD1）、锌指蛋白（A20）及基质金属蛋白酶9（MMP-9）mRNA的表达。结果倒置显微镜下观察：实验组细胞随着氯化镧浓度的升高，细胞密度逐渐降低，细胞质内颗粒逐渐增多，颜色加深，细胞间隙增大，少量细胞变圆漂浮，脱落细胞也逐渐增多；而对照组细胞贴壁生长密集，形态清晰，胞质饱满，相邻细胞生长融合成片。激光共聚焦显微镜观察：实验组细胞核染色质浓聚边集，核体积变小，随着氯化镧浓度的升高，核染色质崩解，核膜破裂、核碎裂，直至细胞核完全碎裂；而对照组细胞核饱满，核膜完整。实验组细胞经不同浓度（5、50、100μmol／L）的氯化镧作用后，细胞生长抑制率分别为24％、51％、78％，高于对照组（0），差异有统计学意义（P〈0．05）；细胞凋亡率分别为（4．91±0．39）％、（7．30±0．71）％、（13．48±0．92）％，高于对照组的（0．89±0．11）％，差异有统计学意义（P〈0．01）；穿膜细胞数分别为（22．2±4．3）、（12．0±3．2）、（7．8±2．6）个，低于对照组的（41．2±5．4）个，差异有统计学意义（P〈0．01）。实验组细胞中cyclinDl、A20及MMP-9mRNA的表达强度随氯化镧浓度（5、50、100μmol／L）的升高逐渐减弱。结论氯化镧通过下调宫颈癌细胞中增殖、抗凋亡和迁移相关基因cyclinD1、A20及MMP-9mRNA的表达，抑制宫颈癌细胞的增殖和迁移并诱导其凋亡。

关键词：宫颈肿瘤镧 HeLa细胞细胞增殖细胞运动细胞凋亡

NF-κB及其调控的miRNA在宫颈癌移植瘤及癌旁组织中的活化和表达

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基础医学与临床 2014年第1期34卷 1-5页

作者：甘自立谌模武林红李勇熊秋迎刘丝荪郭菲南昌大学第一附属医院烧伤中心江西南昌330006 南昌大学研究生院南昌县疾控中心江西南昌330006 南昌大学第一附属医院肿瘤科江西南昌330006 南昌大学第一附属医院病理科南昌大学第一附属医院妇产科

目的分析宫颈癌移植瘤小鼠的癌与癌旁组织中miR-15b和miR-16的表达状况以及NF-κB信号通路在宫颈癌癌旁组织活化情况,为宫颈癌的诊断和治疗提供新思路。方法建立小鼠宫颈癌移植瘤模型,取癌组织与癌旁组织,以及正常的脂肪组织,用实时荧... 详细信息

目的分析宫颈癌移植瘤小鼠的癌与癌旁组织中miR-15b和miR-16的表达状况以及NF-κB信号通路在宫颈癌癌旁组织活化情况,为宫颈癌的诊断和治疗提供新思路。方法建立小鼠宫颈癌移植瘤模型,取癌组织与癌旁组织,以及正常的脂肪组织,用实时荧光定量PCR方法检测miR-15b和miR-16的表达,用免疫组化检测NF-κB(P65)蛋白的表达及定位。结果移植瘤造模成功。miR-15b在宫颈癌组织中表达为32.21±3.67,明显高于癌旁组织的25.16±1.86和正常组织的1.00±0.12(P<0.05),癌旁组织也显著高于正常组织(P<0.05);miR-16在宫颈癌组织中表达为28.63±2.34,明显高于癌旁组织的22.16±1.76和正常组织的1.00±0.12(P<0.05),癌旁组织也显著高于正常组织(P<0.05)。在正常脂肪组织中,NF-κB(P65)蛋白主要定位于胞质,而在癌组织与癌旁组织中,NF-κB(P65)蛋白主要位于胞核。结论 NF-κB通路及其相关的miR-15b和miR-16可能参与了宫颈癌发生的早期分子事件。

关键词：宫颈癌 NF-κB miR-15b miR-16

Plant molecular phylogeography in China and adjacent regions:Tracing the genetic imprints of Quaternary climate and environmental change in the world's most diverse temperate flora

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Plant molecular phylogeography in China and adjacent regions...

后植物志时代的系统与进化植物学——2011年全国系统与进化植物学暨第十届青年学术研讨会

作者： ying-xiong qiu (Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education,and College of Life Sciences,Zhejiang University,Hangzhou 310058,China Laboratory of Systematic & Evolutionary Botany and Biodiversity,Institute of Plant Sciences,and Conservation Center for Gene Resources of Endangered Wildlife,Zhejiang University,China)

The Sino-Japanese Floristic Region(SJFR) of East Asia harbors the most diverse of the world’s temperate flora,and was the most important glacial refuge for its Tertiary representatives(’relics’) throughout Quaternary ice-age cycles.A steadily increasing number of phylogeographic studies in the SJFR of China’s mainland and adjacent areas,including the Qinghai-Tibetan-Plateau(QTP) and Sino-Himalayan region,have documented the population histories of temperate plant species in these *** we review this current literature that challenges the oft-stated view of the SJFR as a glacial

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少见部位间叶性软骨肉瘤5例临床病理分析

诊断病理学杂志 2017年第3期24卷 166-169页

作者：熊振芳胡秀华熊欣熊秋迎熊磊梅金红南昌大学第一附属医院病理科南昌330006 南昌市洪都中医院急诊科南昌330038

目的分析间叶性软骨肉瘤(MC)的临床病理学和免疫组化特点,探讨其诊断、鉴别诊断依据和预后。方法采用HE及免疫组化En Vision两步法对5例MC进行观察,并随访。结果 5例中,女性3例,男性2例。1例原发于骨(左上颌骨),3例原发于软组织,部位分... 详细信息

目的分析间叶性软骨肉瘤(MC)的临床病理学和免疫组化特点,探讨其诊断、鉴别诊断依据和预后。方法采用HE及免疫组化En Vision两步法对5例MC进行观察,并随访。结果 5例中,女性3例,男性2例。1例原发于骨(左上颌骨),3例原发于软组织,部位分别位于盆腔后腹膜、大阴唇和右大腿;1例原发于脑组织(左颅底近舌下神经管区)。临床表现主要为疼痛、肿胀和局部肿块。主要影像学特点为溶骨性破坏或软组织肿块伴肿瘤内钙化和肿瘤边缘硬化。镜下肿瘤由未分化的小圆细胞和肿瘤性透明软骨小岛组成。软骨区的分化程度不等,可以是分化良好的软骨岛,也可以是不成熟的低级别软骨肉瘤。免疫组化:未分化小细胞CD99、Syn、CgA、NSE、vimentin和bcl-2(+);软骨区S-100(+);40%~70%的肿瘤细胞核Ki-67、p53(+)。结论 MC是一种罕见的高度恶性肿瘤,其组织学特点是小细胞原始间叶组织向幼稚软骨组织分化,其诊断要点是在未分化的小细胞区找到分化较成熟的软骨组织。活检小标本容易误诊,须结合组织学特征、影像学表现及免疫组化与其他肿瘤相鉴别。

关键词：软骨肉瘤免疫组化诊断

The Ryukyu Island Arc revisited:nSSR and cpDNA data support lineage divergence in a bird-dispersed tree,Neolitsea sericea(Lauraceae),across a deep sea-barrier during the Latest Pleistocene

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The Ryukyu Island Arc revisited:nSSR and cpDNA data support ...