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Analysis of Occurrence and Mixed Infection of Sugarcane Bacilliform Virus Disease in Hainan Sugarcane-growing Area

Plant Diseases and Pests 2024年第3期15卷 8-11,48页

作者： Linbo SHEN Shuzhen ZHANG Tingting SUN guoru xiong Meidan HE Wenzhi WANG National Key Laboratory for Tropical Crop Breeding Institute of Tropical Bioscience and BiotechnologyChinese Academy of Tropical Agricultural SciencesHaikou 571101China Key Laboratory of Tropical Crop Biology and Genetic Resources Utilization Ministry of Agriculture and Rural Areas/Hainan Institute of Tropical Agricultural ResourcesHainan Key Laboratory of Conservation and Utilization of Tropical Agricultural Bioresources/Sanya Research InstituteChinese Academy of Tropical Agricultural SciencesSanya 572024China Tiangong Residential College Hainan UniversityHaikou 570228China

[Objectives]The paper was to explore the occurrence and mixed infection of sugarcane bacilliform virus disease in Hainan sugarcane-growing area.[Methods]A total of 348 sugarcane leaf samples were collected from 7 sugarcane-growing areas in Hainan *** detection of sugarcane bacilliform virus(SCBV)was carried out by PCR using specific primers.[Results]SCBV was detected in 244 out of 348 sugarcane samples,with an average detection rate of 70.11%.The highest detection rate was 76.66%in the Danzhou sugarcane-growing area,while the lowest was 57.14%in the Baisha sugarcane-growing *** SCBV-positive samples were subjected to testing for SCYLV,SCSMV,SrMV,and SCMV,*** results indicated that 106 out of 244 positive samples exhibited a single infection with SCBV,while 138 samples exhibited mixed infections with SCBV and other sugarcane *** proportion of mixed infections among the SCBV-positive samples was as high as 56.56%.Among the various types of mixed infections,two-virus and three-virus mixed infections were the most prevalent.[Conclusions]SCBV has emerged as a significant threat to the secure production of sugarcane in the Hainan sugarcane-growing *** presents an explosive infection in the Hainan sugarcane-growing region and frequently combines with other sugarcane viruses to infect *** findings of this study will provide a theoretical foundation for the prevention and control of sugarcane bacilliform virus disease.

关键词： Sugarcane Sugarcane bacilliform virus disease Detection rate Mixed infection

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Study on Sugarcane Smut

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Plant Diseases and Pests 2012年第2期3卷 12-14页

作者： guoru xiong Shuzhen ZHANG Institute of Tropical Bioscience and Biotechnology Chinese Academy of Tropical Agricultural Science

The pathogen and characteristics, infection cycle, occurrence regularity and damage symptoms of sugarcane smut were introduced in the paper. Moreover, the comprehensive prevention measures were proposed on the basis of planting disease-resistant species, strengthening cultivation managements to improve dis- ease-resistant ability of sugarcane, and adopting fungicide control as assistant measures. The study provided certain basis for reducing damages of sugarcane smut and imorovin yield and quality of sugarcane.

关键词： Sugarcane smut Infection cycle Occurrence regularity Damage symptoms Prevention and control

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Preliminary Study on Production Conditions and Action Mechanism of Antifuingal Protein from Strain HJX1

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Agricultural Biotechnology 2016年第5期5卷 46-48页

作者： Gengfeng ZHAO Yimin QIU Yunxia ZHANG guoru xiong Hainan Medical University Haikou 571199 China Institute of Tropical Bioscience and Biotechnology Chinese Academy of Tropical Agriculture Sciences (CATAS) Haikou 571101 China

Antagonistic bacterium HJXI is a Bacillus subtilis strain isolated from field rhizasphere soil where banana wih was serious, with certain control efficacy on banana Fusarium wilt. The crude extract obtained from its fermentation broth after ammonium sulfate precipitation has an inhibitory effect on various pathogenic fungi. The culture conditions for this strain were optimized in this study with Fusarium oxysporum f. sp. ettbense as an indicator, and the results showed that the fermentation broth obtained from culture in LB liquid medium under 30 ℃ at 170 r/min for48 h had the strongest inhibition against F. oxysparum f. 8p. cubense. It was observed that the inhibition was mainly reflected by causing breakage and distortion of myeelia, followed by appearance of vesicles and swelling, ablation, and leakage of protoplasm. This study provides a scientific basis for the study on the action mechanism of crude protein from bacterial strain HJX1.

关键词： Antagonistic bacteria Banana Fusarium wilt Antimierobial protein Inhibition

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Identification of Biocontrol Strain HJX1 and Determination of Its Antifungal Spectrum

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Plant Diseases and Pests 2014年第3期5卷 11-14,19页

作者： Zhao Gengfeng Qiu Yimin Zhang Yunxia xiong guoru College of Science Hainan Medical College Institute of Tropical Biotechnology Chinese Academy of Tropical Agricultural Sciences

A biocontrol strain HJX1,which had good inhibitory effect against Fusarium oxysporum f. sp. cubense,was identified as Bacillus subtilis based on traditional morphology,16 S rRNA gene sequence and fatty acid profiles. The sequence of 16 S rRNA gene had 99% homology to B. subtilis according to the DNA fragment of 1 521 bp amplified with a pair of universal primers,P0 and P6,of bacteria. Confront culture with pathogenic fungi showed that this strain had good inhibitory effect against 10 pathogenic fungi.

关键词： Bacillus subtilis HJX1 Identification Fusarium oxysporum f.sp.cubense Biological control

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Fungistatic Activity of Crude Protein Extracted from Bacillus subtilis HJX1

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Plant Diseases and Pests 2015年第4期6卷 22-24页

作者： Zhao Gengfeng Qiu Yimin Zhang Yunxia xiong guoru Department of Science Hainan Medical University Institue of Tropical Bioscience and Biotechnology Chinese Academy of Tropical Agricultural Sciences

Bacillus subtilis HJX1, a biocontrol strain of Fusarium oxysporum f. sp. cubense, could inhibit growth of several plant pathogenic fungi in vitro. To un- derstand its mechanism of fungistasis, we extracted crude protein from fermentation broth of the strain HJX1 through ammonium sulfate precipitation, and prelimina- rily studied fungistatic activities at different conditions. The results showed that the crude protein was insensitive to protease K, trypsin or ultraviolet radiation. The fungistatic activity was unchanged when treated in water batch at 40 ℃, 60 ℃, 80 ℃ or 100 ℃ for 30 min, and the fungistatic activity maintained 60% when trea- ted at 121℃ for 30 min.

关键词： Bacillus subtilis HJX1 Fungistatic protein Activity Fusarium oxysporum f. sp. cubense

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Optimization of Streptomyces caelestis Culture Conditions and Preliminarily Determination of Its Antifungal Components

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Agricultural Biotechnology 2018年第5期7卷 256-258,263页

作者： Gengfeng ZHAO guoru xiong Wenzhi WANG Linbo SHEN Shuzhen ZHANG Institute of Tropical Bioscience and Biotechnology Chinese Academy of Tropical Agricultural Sciences College of Basic Medicine and Life Science Hainan Medical University

Streptomyces caelestis FAS is an actinomyces strain isolated with Ustilago scitaminea as an indicator. With GAUZE s medium as the basic medium, carbon source and nitrogen source in the medium and ventilation volume were optimized, and the antifungal substances produced by S. caelestis FAS were preliminarily determined through organic solvent extraction and ammonium sulfate precipitation. The results showed that the antifungal components produced by S. caelestis FAS were proteins, and the optimal culture conditions were using corn flour as carbon source and KNO 3 as nitrogen source and culturing in 250 ml triangular flask added with 70 ml of culture medium. The crude protein obtained under these culture conditions had the best antifungal effect with an inhibition zone diameter of 25 mm. This study will provide a scientific basis for further study on the antifungal mechanism and application of S. caelestis FAS.

关键词： Streptomyces caelestis FAS Sugarcane smut Culture conditions Antifungal components

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Isolation,Screening and Identification of Antagonistic Actinomycetes against Ustilago scitaminea Syd

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Agricultural Biotechnology 2018年第4期7卷 225-228页

[Objectives] This study was conducted to obtain actinomycetes strains having antagonistic effect on Ustilago scitaminea Syd.[Methods] At first, actinomycetes strains were isolated from 22 soil samples in Hainan sugarcane regions. Then, antagonistic actinomycetes against U. scitaminea were screened by confrontation culture. Finally, the taxonomic status of antagonistic actinomycetes was determined using 16S rDNA.[Results] From the 22 samples, 984 actinomycetes strains were isolated. From all the isolated strains, 23 antagonistic actinomycetes strains were obtained through primary screening, and one strains with better antagonistic effect was then obtained through secondary screening, and designated FAS. 16S rDNA identification showed that strain FAS shared 99% sequence similarity with Streptomyces cealestis US24. A phylogenetic tree was built with MAGE 7.0 software, and the results showed that strain FAS had the shortest genetic distance with S. caelestis US24. Therefore, the actinomycetes FAS was determined as S. caelestis .[Conclusions] This study provides a new biocontrol method for the biological control of sugarcane smut, thereby ensuring sustainable development of sugarcane industry and sugar industry.

关键词： Sugarcane smut 16S rDNA identification Streptomyces caelestis Antagonistic effect

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Study on Acute Toxicity of Purified Protein HAS1 Expression in KM Mice

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Agricultural Biotechnology 2019年第4期8卷 69-71页

作者： guoru xiong Gengfeng ZHAO Jungang WANG Guilan XING Shuzhen ZHANG Institute of Tropical Bioscience and Biotechnology CATAS/Hainan Academy of Tropical Agricultural ResourceCATAS/Sugarcane Research CenterHaikou 571101China College of Basic Medicine and Life Science Hainan Medical CollegeHaikou 571101China Research Center for Drug Safety Evaluation of Hainan Province Haikou 571101China

[Objectives]The antifungal protein HAS1 newly obtained was evaluated for acute toxicity safety in KM mice according to relevant national regulations,so as to eliminate people s concerns about the safety of transgenic plants.[Methods]The acute toxicity of the purified protein HAS1 was observed by intragastric administration of mice,and the poisoning symptoms,poisoning degree,recovery and death were observed.[Results]No abnormal toxicity symptoms were observed in the test group,the vehicle control group and the blank control *** main tissues and organs were not abnormal in gross *** average body weight of each group showed an increasing trend compared with before administration at 1,3,7,11 and 14 d after *** was found that after giving the purified protein HAS1 to KM mice at a cumulative dose of 64 mg/kg a day,no obvious toxicity was observed in the acute toxicity test,indicating that the test substance was non-toxic by oral administration.[Conclusions]This study provides a basis for further use of the protein and its coding genes.

关键词： Bacillus subtilis HAS Purified protein HAS1 KM mice Toxic reaction

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Identification of In-intro Antagonistic Activity of HAS1 Protein against Sporisorium scitanminea

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Agricultural Biotechnology 2018年第6期7卷 9-11,15页

作者： Huiqing LIU Yuantao HUANG Mengyu DAI Yimin QIU Gengfeng ZHAO guoru xiong College of Basic Medicine and Life Science Hainan Medical University Institute of Tropical Bioscience and Biotechnology Chinese Academy of Tropical Agricultural Sciences

Sugarcane smut greatly influences yield and quality of ***1 gene in biocontrol strain HAS antagonistic to Sporisorium scitanminea was selected for prokaryotic expression vector construction and inducible expression,and the antagonistic effect of HAS1 protein was detected in *** results showed that the expression product of antifungal HAS1 gene obtained through prokaryotic expression vector construction and inducible expression had better antagonistic effect against *** study provides an experimental basis for further utilization of this protein.

关键词： Bacillus subtilis HAS Sporisorium scitanminea Prokaryotic expression Antifungal protein

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Study on Wide Antifungal Property of Bacillus subtilis HAS

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Agricultural Biotechnology 2018年第3期7卷 116-117,121页

作者： guoru xiong Gengfeng ZHAO Wenzhi WANG Linbo SHEN Xiaoyan FENG Shuzhen ZHANG Institute of Tropical Bioscience and Biotechnology Chinese Academy of Tropical Agricultural Science College of Basic Medicine and Life Science Hainan Medical College

Bacillus subtilis HAS is a biocontrol strain with better inhibitory effect on Sporisorium scitaminea Sydow. In order to further understand its action range,with fungal pathogens in sugarcane production as targets,confrontation tests and tests of inhibitory effect of secondary metabolites on growth of pathogens were carried out. The results showed that B. subtilis HAS not only had better inhibitory effect on S. Scitaminea,but also inhibited the growth of various fungal pathogens in sugarcane production. The inhibitory effect was the best on Ceratocystis adiposum,Thielaviopsis paradoxa,Fusarium moniliforme and Colletotrichum fuleatum,which was reflected by the radius of inhibitory zone over 10 mm. The inhibitory rates of the secondary metabolites were about 70%. It indicates that B. subtilis HAS has wide antifungal property,providing reference for further application of this strain.

关键词： Bacillus subtilis HAS Confrontation training Secondary metabolites Inhibitory eft^ct

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