Study on Acute Toxicity of Purified Protein HAS1 Expression in KM Mice

作     者：Guoru XIONG Gengfeng ZHAO Jungang WANG Guilan XING Shuzhen ZHANG 

作者机构：Institute of Tropical Bioscience and BiotechnologyCATAS/Hainan Academy of Tropical Agricultural ResourceCATAS/Sugarcane Research CenterHaikou 571101China College of Basic Medicine and Life ScienceHainan Medical CollegeHaikou 571101China Research Center for Drug Safety Evaluation of Hainan ProvinceHaikou 571101China 

出 版 物：《Agricultural Biotechnology》 (农业生物技术（英文版）)

年 卷 期：2019年第8卷第4期

页      面：69-71页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：Supported by National Natural Science Foundation(31471555) National Key Research and Development Project(SQ2018YFD020024) 

主　　题：Bacillus subtilis HAS Purified protein HAS1 KM mice Toxic reaction 

摘      要：[Objectives]The antifungal protein HAS1 newly obtained was evaluated for acute toxicity safety in KM mice according to relevant national regulations,so as to eliminate people s concerns about the safety of transgenic plants.[Methods]The acute toxicity of the purified protein HAS1 was observed by intragastric administration of mice,and the poisoning symptoms,poisoning degree,recovery and death were observed.[Results]No abnormal toxicity symptoms were observed in the test group,the vehicle control group and the blank control *** main tissues and organs were not abnormal in gross *** average body weight of each group showed an increasing trend compared with before administration at 1,3,7,11 and 14 d after *** was found that after giving the purified protein HAS1 to KM mice at a cumulative dose of 64 mg/kg a day,no obvious toxicity was observed in the acute toxicity test,indicating that the test substance was non-toxic by oral administration.[Conclusions]This study provides a basis for further use of the protein and its coding genes.