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Performance of microbiological control by a point-of-use filter system for drinking water purification

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Journal of Environmental Sciences 2009年第9期21卷 1237-1246页

作者： SU Fengyi LUO Mingfang ZHANG Fei LI Peng LOU Kai xing xinhui Department of Chemical Engineering Tsinghua University Beijing 100084 China. College of Chemistry and Chemical Engineering Graduate University of Chinese Academy of Sciences Beijing 100049 China Institute of Microbiology Xinjiang Academy of Agricultural Sciences Urumqi 830000 China

Purification capacity of a faucet mounted type water filter for home use was evaluated, particularly with regard to microbiological performance under different running conditions. Biofilms were formed inside the filter, affecting the bacterial quality of the effluent water. Low flow rate, long stagnation period and high filter temperature were found favorable for bacterial growth inside. By commercial analytical profile index （API） kits, ten different bacterial species were identified in drinking water, four of which were probably contributed to the biofilm formation since they were also present in the biofilm. Fluorescence in situ hybridization （FISH） was used to confirm the API identification results, and direct viable count （DVC） method was employed to improve the sensitivity of FISH for the isolated Acinetobacter spp. and Pseudomonas putida as models. Relationship between the filter operating condition and the bacterial community alteration was partly revealed, which could provide the basic knowledge for the filter design and its practical use.

关键词： drinking water point-of-use filter biofilm bacteria community activated carbon

Quantitative evaluation of DNA damage caused by atmospheric and room-temperature plasma (ARTP) and other mutagenesis methods using a rapid umu-microplate test protocol for microbial mutation breeding

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Chinese Journal of Chemical Engineering 2021年第11期34卷 205-210页

作者： Yuting Huang Liyang Wang Xue Zhang Nan Su Heping Li Yoshimitsu Oda xinhui xing Key Laboratory for Industrial Biocatalysis Ministry of Education of ChinaDepartment of Chemical EngineeringTsinghua UniversityBeijing 100084China Biobreeding Research Center Wuxi Research Institute of Applied TechnologiesTsinghua UniversityWuxi 214072China Department of Entomology College of Plant ProtectionChina Agricultural UniversityBeijing 100193China Department of Engineering Physics Tsinghua UniversityBeijing 100084China Institute of Life and Environmental Sciences Osaka Shin-Ai CollegeTsurumiTsurumi-kuOsaka 5380052Japan Centre for Synthetic and Systems Biology Tsinghua UniversityBeijing 100084China Institute of Biopharmaceutical and Health Engineering Tsinghua Shenzhen International Graduate SchoolShenzhen 518055China Institute of Biomedical Health Technology and Engineering Shenzhen Bay LaboratoryShenzhen 518055China

Mutagenesis is an important technique for microbial mutation *** the source of mutations,DNA damage extent is a key indicator for the effectiveness of ***,a rapid and easy DNA damage quantification method is required for the comparison of mutagenesis effects and development of mutagenesis ***,we used the umu-microplate test system to quantitatively compare the DNA damage strength caused by atmospheric and room-temperature plasma(ARTP)and other traditional mutagenesis methods including:ultraviolet radiation(UV),diethyl sulfate(DES)and 4-nitroquinoline-1-oxide(4-NQO).The test strain of Salmonella typhimurium TA1535/pSK1002 was used to monitor the time-course profile of b-galactosidase activity induced by DNA damage caused by different mutagenesis methods using a microplate *** umu-microplate test results showed that ARTP caused higher extent of DNA damage than UV and chemical mutagens,which agrees well with the result obtained by SOS-FACS-based quantification method as reported *** umu-microplate test is accessible for broad researchers who are lack of the expensive FACS instruments and allows the quick quantitative evaluation of DNA damage among living cells for different mutagenesis methods in the study of the microbial mutation breeding.

关键词： ARTP mutagenesis umu-Microplate test Biological engineering Cell engineering Biotechnology

Combinatorial co-expression of xanthine dehydrogenase and chaperone XdhC from Acinetobacter baumannii and Rhodobacter capsulatus and their applications in decreasing purine content in food

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Food Science and Human Wellness 2023年第4期12卷 1343-1350页

作者： Chenghua Wang Ran Zhang Yu Sun You Wen Xiaoling Liu xinhui xing College of Light Industry and Food Engineering Guangxi UniversityNanning 530004China Key Laboratory of Industrial Biocatalysis Ministry of EducationInstitute of Biochemical EngineeringDepartment of Chemical EngineeringTsinghua UniversityBeijing 100084China

This study investigated the combinatorial expression of xanthine dehydrogenase(XDH)and chaperone XdhC from Acinetobacter baumannii and Rhodobacter capsulatus and their applications in decreasing purine content in the beer,beef and *** occurring xdhABC gene clusters of *** CICC 10254 and *** CGMCC 1.3366 as well as two refactored clusters constructed by exchanging their xdhC genes were overexpressed in Escherichia coli and purified to near *** chaperoned by AbXdhC showed nearly the same catalytic performance as that by RcXdhC,except for the decreased substrate *** the AbXDH co-expressed with RcXdhC displayed enhanced acidic adaptation but weakened catalytic *** the XDHs degraded purines in beer,beef and yeast extract effectively,indicating potential applications in low-purine foods to prevent hyperuricemia and *** study also presents a method for exploiting the better chaperone XdhC and novel XDHs by functional complement activity using existing XdhCs such as RcXdhC.

关键词： Co-expression Low purine food Uric acid Xanthine dehydrogenase XdhC

Exploring the Trans-Cleavage Activity with Rolling Circle Amplification for Fast Detection of miRNA

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BioDesign Research 2023年第1期5卷 72-79页

作者： Chenqi Niu Juewen Liu xinhui xing Chong Zhang MOE Key Laboratory for Industrial Biocatalysis Institute of Biochemical EngineeringDepartment of Chemical EngineeringTsinghua UniversityBeijing 100084China. Department of Chemistry Waterloo Institute for NanotechnologyUniversity of Waterloo200 University Avenue WestWaterlooON N2L 3G1Canada. Center for Synthetic and Systems Biology Tsinghua UniversityBeijing 100084China.

MicroRNAs(miRNAs)are a class of endogenous short noncoding *** regulate gene expression and function,essential to biological *** is necessary to develop an efficient detection method to determine these valuable biomarkers for the diagnosis of *** this paper,we proposed a general and rapid method for sensitive and quantitative detection of miRNA by combining CRISPR–Cas12a and rolling circle amplification(RCA)with the precircularized ***,the detection of miRNA-21 could be completed in 70 min with a limit of detection of 8.1 pM with high *** reaction time was reduced by almost 4 h from more than 5 h to 70 min,which makes detection more *** design improves the efficiency of CRISPR–Cas and RCA-based sensing strategy and shows great potential in lab-based detection and point-of-care test.

关键词： diagnosis specificity endogenous

Internal connections between dietary intake and gut microbiota homeostasis in disease progression of ulcerative colitis:a review

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Food Science and Human Wellness 2021年第2期10卷 119-130页

作者： Wen Zeng Dong He Yifan xing Junyu Liu Nan Su Chong Zhang Yi Wang xinhui xing MOE Key Laboratory for Industrial Biocatalysis Institute of Biochemical EngineeringDepartment of Chemical EngineeringTsinghua UniversityBeijing 100084China Centre for Synthetic and Systems Biology Tsinghua UniversityBeijing 100084China Institute of Biopharmaceutical and Health Engineering Tsinghua Shenzhen International Graduate SchoolShenzhen 518055China

Ulcerative colitis(UC)is a chronic systematic inflammation disorder with increasing incidence,unknown pathogenesis,limited drug treatment,and abundant medical *** intake,as a daily indispensable environment factor,is closely related to UC pathogenesis and *** underlying interactions between dietary intake and UC progression are implicated with the modulation of gut microbiome as well as microbial metabolites,suggesting the complex and systematic characteristics of ***,the triangular relationships with dietary intake,gut microbiota homeostasis,and UC have not been well summarized so *** we review the recent studies of dietary intake on the regulation of gut microbiome homeostasis as well as modulation of UC *** findings suggest that varieties in dietary patterns result in the production of diverse microbial fermentation metabolites,which contribute to gut microbiome homeostasis through multiple manipulations including immune modulation,inftammation restriction as well as epithelial barrier maintenance,thus finally determine the fate of UC progression and give implications for functional food development for prevention and treatment of UC patients.

关键词： Ulcerative colitis Dietary intake Gut microbiome Metabolite Homeostasis

Isolation and characterization of novel peptides from fermented products of Lactobacillus for ulcerative colitis prevention and treatment

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Food Science and Human Wellness 2022年第6期11卷 1464-1474页

作者： Dong He Wen Zeng Yi Wang Yifan xing Kang Xiong Nan Su Chong Zhang Yuan Lu xinhui xing School of Chemical Engineering and Light Industry Guangdong University of TechnologyGuangzhou 510006China Key Laboratory for Industrial Biocatalysis Ministry of EducationInstitute of Biochemical EngineeringDepartment of Chemical EngineeringChina Center for Synthetic and Systems BiologyTsinghua UniversityBeijing 100084China Center for Synthetic and Systems Biology Tsinghua UniversityBeijing 100084China Institute of Biopharmaceutical and Health Engineering Tsinghua Shenzhen International Graduate SchoolShenzhen 518055China Institute of Biomedical Health Technology and Engineering Shenzhen Bay LaboratoryShenzhen 440300China

Ulcerative colitis(UC)is an incurable and highly complex digestive disease affecting millions of people *** to the current therapeutic drugs,bioactive peptides are more promising and safe substances as functional foods or drugs for the prevention and treatment of *** alcohol-soluble components from fermentation broth by fresh wheat germ and apple(AC-WGAF)were found to be effective in UC prevention in dextran sulfate sodium-induced mice in ***,4 novel peptides are identifi ed from AC-WGAF by membrane ultrafi ltration,recycling preparative high-performance liquid chromatography,and matrix-assisted laser desorption–ionization time-of-fl ight/time-of-fl ight mass spectrometry,possessing anticolitis activity via using an in vitro *** of those peptides named T24(PVLGPVRGPFPLL)exhibited the most remarkable anti-colitis activity by preventing tight junction protein loss,maintaining epithelial barrier integrity,and promoting cell proliferation during in vitro and in vivo studies by regulating mitogen-activated protein kinase signaling ***,T24 is a promising peptide as a functional food or novel drug for UC prevention and treatment.

关键词： Ulcerative colitis Peptide Fermentation product Intestinal epithelial barrier Cell proliferation Mitogen-activated protein kinase signaling pathways

Emerging natural hemp seed proteins and their functions for nutraceutical applications

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Food Science and Human Wellness 2023年第4期12卷 929-941页

作者： Haihong Chen Bing Xu Yi Wang Wei Li Dong He Yan Zhang Xizhen Zhang xinhui xing Institute of Biopharmaceutical and Health Engineering Tsinghua Shenzhen International Graduate SchoolShenzhen 518055China Institute of Biomedical Health Technology and Engineering Shenzhen Bay LaboratoryShenzhen 440300China Key Laboratory for Industrial Biocatalysis Ministry of EducationInstitute of Biochemical EngineeringDepartment of Chemical EngineeringTsinghua UniversityBeijing 100084China School of Chemical Engineering and Energy Technology Engineering Research Center of Health Food Design&Nutrition RegulationKey Laboratory of Healthy Food Development and Nutrition Regulation of China National Light IndustryDongguan University of TechnologyDongguan 523808China Hebei Food Inspection and Research Institute Shijiazhuang 050091China College of life Science and Technology Guangxi UniversityNanning 530004China Center for Synthetic and Systems Biology Tsinghua UniversityBeijing 100084China

With changing dietary habits and increasing awareness of the nutraceutical role of dietary foods,the demand for natural plant proteins and interest in non-traditional protein sources in the food industry are *** hemp,belonging to the plant family Cannabaceae,is cultivated for its fibre and edible *** to its nutritional value,it has also been used in the food industry and *** particular,hemp seed proteins have drawn considerable attention in both scientific and industrial fields because of their excellent nutraceutical values,superior digestibility,low allergenicity and diverse techno-functional *** this review,we provide a summary of the current research progress on the extraction and purification processes,physiochemical properties,nutraceutical functions,and applications of hemp seed *** in the application of advanced technologies for hemp seed bioactive peptide mining are also *** review provides up-to-date insights into the nutraceutical values,health benefits,and future applications of this emerging plant source protein.

关键词： Hemp seed protein Extraction Purification Physicochemical properties Nutraceutical functions Food industry

Genome-scale metabolic models applied for human health and biopharmaceutical engineering

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Quantitative Biology 2023年第4期11卷 363-375页

作者： Feiran Li Yu Chen Johan Gustafsson Hao Wang YiWang Chong Zhang xinhui xing nstitute of Biopharmaceutical and Health Engineering Tsinghua Shenzhen International Graduate SchoolTsinghua UniversityShenzhenChina Key Laboratory of Quantitative Synthetic Biology Shenzhen Institute of Synthetic BiologyShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhenChina Department of Biology and Biological Engineering Chalmers University of TechnologyGothenburgSweden Key Laboratory for Industrial Biocatalysis Ministry of EducationInstitute of Biochemical EngineeringDepartment of Chemical EngineeringTsinghua University BeijingChina Center for Synthetic and Systems Biology Tsinghua UniversityBejingChina Institute of Biomedical Health Technology and Engineering Shenzhen Bay LaboratoryShenzhenChina

Over the last 15 years,genome-scale metabolic models(GEMs)have been reconstructed for human and model animals,such as mouse and rat,to systematically understand metabolism,simulate multicellular or multi-tissue interplay,understand human diseases,and guide cell factory design for biopharmaceutical protein ***,we describe how metabolic networks can be represented using stoichiometric matrices and well-defined constraints for flux ***,we review the history of GEM development for quantitative understanding of Homo sapiens and other relevant animals,together with their *** describe how model develops from *** to other animals and from generic purpose to precise context-specific *** progress of GEMs for animals greatly expand our systematic understanding of metabolism in human and related *** discuss the difficulties and present perspectives on the GEM development and the quest to integrate more biological processes and omics data for future research and *** truly hope that this review can inspire new models developed for other mammalian organisms and generate new algorithms for integrating big data to conduct more in-depth analysis to further make progress on human health and biopharmaceutical engineering.

关键词： constraint-based modeling disease genome-scale metabolicmodel metabolism

Transcriptomic analysis of Andrias davidianus meat and experimental validation for exploring its bioactive components as functional foods

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Food Science and Human Wellness 2024年第1期13卷 166-172页

作者： Changge Guan Zhenglin Tan Shucheng Li Yi Wang Naoyuki Yamamoto Chong Zhang Songjun Wang Junjie Chen xinhui xing Key Laboratory for Industrial Biocatalysis Ministry of Education of ChinaDepartment of Chemical EngineeringTsinghua UniversityBeijing 100084China School of Life Science and Technology Tokyo Institute of TechnologyYokohama 226-0026Japan Sensing System Research Center National Institute of Advanced Industrial Science and TechnologyTsukuba 305-8565Japan Center for Synthetic and Systems Biology Tsinghua UniversityBeijing 100084China Henan Giant Salamander Protection and Development Association Luoyang 471000China Intelligent Computing Research Center Harbin Institute of TechnologyShenzhen 518071China Institute of Biopharmaceutical and Health Engineering Tsinghua Shenzhen International Graduate SchoolShenzhen 518055China Institute of Biomedical Health Technology and Engineering Shenzhen Bay LaboratoryShenzhen 518067China

Andrias davidianus(Chinese giant salamander,CGS)is the largest and oldest extant amphibian species in the world and is a source of prospective functional food in ***,the progress of functional peptides mining was slow due to lack of reference genome and protein sequence *** this study,we illustrated full-length transcriptome sequencing to interpret the proteome of CGS meat and obtain 10703 coding DNA *** functional annotation and amino acid composition analysis,we have discovered various genes related to signal transduction,and 16 genes related to *** have also found vast variety of functional peptides through protein coding sequence(CDS)analysis by comparing the data obtained with the functional peptide ***-Pro-Ile predicted by the CDS analysis was released from the CGS meat through enzymatic hydrolysis,suggesting that our approach is *** study suggested that transcriptomic analysis can be used as a reference to guide polypeptide mining in CGS meat,thereby providing a powerful mining strategy for the bioresources with unknown genomic and proteomic sequences.

关键词： Chinese giant salamander Transcriptomic analysis Bioactive components Functional peptides mining