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Methyltransferase Dot1l preferentially promotes innate IL-6 and IFN-β production by mediating H3K79me2/3 methylation in macrophages

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Cellular & Molecular Immunology 2020年第1期17卷 76-84页

作者： Xiang Chen xingguang Liu Yunkai zhang Wanwan Huai Qingqing Zhou Sheng Xu Xi Chen Nan Li Xuetao Cao National Key Laboratory of Medical Immunology&Institute of Immunology Second Military Medical UniversityShanghai 200433China Institute of Immunology Zhejiang University School of MedicineHangzhou 310058China Department of Immunology&Center for Immunotherapy Institute of Basic Medical SciencesPeking Union Medical CollegeChinese Academy of Medical SciencesBeijing 100005China

Epigenetic modification,including histone modification,precisely controls target gene *** posttranscriptional regulation of the innate signaling-triggered production of inflammatory cytokines and type I interferons has been fully elucidated,whereas the roles of histone modification alteration and epigenetic modifiers in regulating inflammatory responses need to be further ***/tri-methylation modifications of histone 3 lysine 79(H3K79me2/3)have been shown to be associated with gene transcriptional *** of telomeric silencing-1-like(Dot1l)is the only known exclusive H3K79 methyltransferase and regulates the proliferation and differentiation of tumor ***,the roles of Dot1l and Dot1l-mediated H3K79 methylation in innate immunity and inflammatory responses remain ***,we found that H3K79me2/3 modification levels at the Il6 and Ifnb1 promoters,as well as H3K79me2 modification at the Tnfαpromoter,were increased in macrophages activated by Toll-like receptor(TLR)ligands or virus *** innate signals upregulated Dot1l expression in macrophages and THP1 ***1l silencing or a Dot1l inhibitor preferentially suppressed the production of IL-6 and interferon(IFN)-βbut not of TNFαin macrophages and THP1 cells triggered by TLR ligands or virus ***1l was recruited to the proximal promoter of the Il6 and Ifnb1 but not Tnfαgene and then mediated H3K79me2/3 modification at the Il6 and Ifnb1 promoters,consequently facilitating the transcription and expression of Il6 and ***,Dot1l-mediated selective H3K79me2/3 modifications at the Il6 and Ifnb1 promoters are required for the full activation of innate immune *** finding adds new insights into the epigenetic regulation of inflammatory responses and pathogenesis of autoimmune diseases.

关键词： H3K79me2/3 Dot1l epigenetic regulation IL-6 IFN-β macrophages inflammation

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Effect of Tongluojiunao injection made from Sanqi(Radix Notoginseng) and Zhizi(Fructus Gardeniae) on brain microvascular endothelial cells and astrocytes in an in vitro ischemic model

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Journal of Traditional Chinese Medicine 2014年第6期34卷 725-732页

作者： Weihong Li xingguang Li Qinghong Du Feng Li Yuan zhu Yang Liu Jie Ma Liangqin Wan Fanghe Li Sai zhang School of Preclinical Medicine Beijing University of Chinese Medicine Encephalopathy Center Jiangsu Province Hospital of Traditional Chinese Medicine Medical Affairs Novartis Pharmaceuticals (China)

OBJECTIVE: To explore the effect of Tongluojiunao injection(TLJN) prepared with Sanqi(Radix Notoginseng) and Zhizi(Fructus Gardeniae) on the interaction between brain microvascular endothelial cells(BMECs) and astrocytes in an in vitro ischemic ***: First, an in vitro model of cerebral ischemia in BMECs or astrocytes was established by oxygen-glucose deprivation(OGD). TLJN was used as a medicine of intervention. The OGD-injuredBMECs were cultured in various astrocyte-conditioned media. Cell activity, alkaline phosphatase(AKP) and γ-glutamyl transpeptidase(γ-GT) activity,interleukin-1 beta(IL-1β), and tumor necrosis factor alpha(TNF-α) content in BMECs were ***, OGD-injured astrocytes were cultured in various BMEC-conditioned media. Cell activity, as well as expression of brain-derived neurotrophic factor(BDNF) and glial cell-derived neurotrophic factor(GDNF) in astrocytes, were ***: The results of paracrine signaling of normal BMECs or astrocytes showed a protective effect on each other: conditioned media from normal astrocytes improved cell viability, AKP, and γ-GT activity, and reduced IL-1β and TNF-α content of injured BMECs; conditioned media from normal BMECs improved cell viability and expression of BDNF and GDNF in injured astrocytes. However, once the BMECs or astrocytes were injured by OGD, the protective effect decreased or disappeared. The above-mentioned protective induction was effectively recovered by TLJN ***: The therapeutic benefit of TLJN was achieved by recovering two-way induction between BMECs and astrocytes, enhancing activity of injured BMECs and astrocytes, stabilizing enzymatic barriers, promoting expression of neurotrophic factors, and inhibiting inflammatory cytokines.

关键词：脑微血管内皮细胞星形胶质细胞模型制作脑缺血胶质细胞源性神经营养因子注射液体外 Lial

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路政与河政的纠葛:以郑州黄河铁路大桥为中心(1903—1937)

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史学月刊 2023年第3期 73-90页

作者：王星光张裕童郑州大学历史学院河南郑州450001

清末郑州黄河铁路大桥的兴建,引发了黄河下游铁路与河防的冲突。黄河的河道、堤防、两岸地质特征,促使铁桥规划避开了下游开封河段,选址郑州。黄河铁桥的工程设计缺陷,更使得路政与河政之间的矛盾激化。铁桥上游北岸的堤防与抛石护墩问... 详细信息

清末郑州黄河铁路大桥的兴建,引发了黄河下游铁路与河防的冲突。黄河的河道、堤防、两岸地质特征,促使铁桥规划避开了下游开封河段,选址郑州。黄河铁桥的工程设计缺陷,更使得路政与河政之间的矛盾激化。铁桥上游北岸的堤防与抛石护墩问题,成为铁路部门与河务部门的冲突焦点。在黄河铁桥兴建、维护中,双方经历了从对峙到合作,从独行到共商的历史过程,初步建立起河防与路政兼顾的合作模式。铁桥工程引发的路政与河政纠葛,蕴含着新旧思想、技术、中西文化、中央与地方的多重冲突,映射出黄河治理的传统要务与近代中国交通革新及维新图强的演变轨迹。

关键词：近代中国郑州黄河铁路大桥京汉铁路路政河防

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Synthesis of CNT film on the surface of micro-pyramid array and its intense pulsed emission characteristics

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Chinese Science Bulletin 2012年第14期57卷 1739-1742页

作者： ZENG FanGuang LI Xin LIU WeiHua QIAO ShuZhen MA HuaLi zhang Rui XIA LianSheng CHEN Yi LIU xingguang zhang Huang Department of Mathematics and Physics Zhengzhou Institute of Aeronautical lndustry Management Zhengzhou 450015 China School of Electronic and Information Engineer-ing Xi＇an Jiaotong University Xi＇an 710049 China Institute of Fluid Physics Chinese Academy of Engineering Physics Mianyang 621900 China

We developed a new scheme to suppress the electric-field-screening effect in high growth density of a carbon nanotube(CNT) film during its intense pulsed *** synthesize the CNT film on a tridimensional surface(t-CNT film).The tridimensional surface includes wet etched silicon pyramids,and the Ni layer is electroless plated *** intense pulsed emission characteristics of the t-CNT and planar-grown CNT(p-CNT) films were measured using a diode structure in single-pulse *** even turn-on field decreased from 5.5 V/μm for p-CNTs to 2.8 V/m for t-CNTs,and the peak emission current increased from 232 A for p-CNTs to 324 A for t-CNTs at a peak field intensity ～12.2 V/*** peak current of the t-CNT film increased by ～39.7% over the p-CNT *** is clear that the micro-pyramid array can effectively suppress the field screening effect to improve the electron-emission of CNT films.

关键词：碳纳米管膜发射特性强流脉冲金字塔微阵列合成表面碳纳米管薄膜

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Improvement in the intense pulsed emission stability of grown CNT films via an electroless plated Ni layer

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Chinese Science Bulletin 2011年第22期56卷 2379-2382页

作者： ZENG FanGuang LI Xin LIU WeiHua QIAO ShuZhen MA HuaLi zhang Rui XIA LianSheng CHEN Yi LIU xingguang zhang Huang Department of Mathematics and Physics Zhengzhou Institute of Aeronautical Industry Management Zhengzhou 450015 China School of Electronic and Information Engineering Xi'an Jiaotong University Xi'an 710049 China Institute of Fluid Physics Chinese Academy of Engineering Physics Mianyang 621900 China

Carbon nanotube(CNT)films were grown on silicon wafers with and without a nickel layer(Si-CNT and Ni-CNT)via the pyrolysis of iron *** nickel layer was prepared using the electroless plating *** study the emission stability of Si-CNT and Ni-CNT cathodes during intense pulsed emission,emission characteristics were measured repeatedly with a diode structure using a Marx generator as a voltage *** the peak values of the pulsed voltage,which were in the range between 1.62-1.66 MV(corresponding to electric field intensities between 11.57-11.85 V/μm),the first cycle emission current was 109.4 A for Si-CNT and 180.5 A for *** comparing the normalized emission currents of the Si-CNT and Ni-CNT cathodes,the improvement in the emission stability can be easily *** number of emission cycles necessary for the peak current to decay from 100%to 50%increased from^3 for Si-CNT to^11 for a Ni-CNT film.

关键词：碳纳米管薄膜化学镀镍层发射电流稳定性强脉冲生长碳纳米管阴极 Marx发生器

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抗生素对结直肠息肉氩离子凝固术及电凝电切术后炎性指标的影响

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武警医学 2018年第7期29卷 653-655,659页

作者：张兴光谢立群邓全军赵建业赵军艳陈金梅耿丽媛武警后勤学院附属医院消化内科天津300162

目的探讨抗生素对结直肠息肉氩离子凝固术及电凝电切术后炎性指标的影响。方法采取前瞻性随机对照研究方法,选择2014-01至2015-12医院消化内科收治的58例结直肠息肉治疗患者,随机分为抗生素组(28例)及非抗生素组(30例)。于术前,术后1、... 详细信息

目的探讨抗生素对结直肠息肉氩离子凝固术及电凝电切术后炎性指标的影响。方法采取前瞻性随机对照研究方法,选择2014-01至2015-12医院消化内科收治的58例结直肠息肉治疗患者,随机分为抗生素组(28例)及非抗生素组(30例)。于术前,术后1、3 d测定血浆内毒素、降钙素原及C-反应蛋白水平。结果 58例治疗后均未出血及穿孔,术后9例出现腹痛、腹胀不适(15.5%),12~24 h症状基本消失。抗生素组患者治疗后出现低热1例(3.6%),发热时间为术后8 h左右,非抗生素组患者治疗后出现发热4例(13.3%),均为低热,发热时间为术后7~14 h,两组发热率差异有统计学意义(P<0.05)。两组治疗后1、3 d与术前比较,血浆内毒素、血浆降钙素原、C-反应蛋白水平变化差异均无统计学意义。结论结直肠息肉氩离子凝固术及电凝电切术治疗在整体上是安全的,治疗后炎性指标基本正常,不支持应用抗生素。

关键词：结直肠息肉内镜治疗内毒素降钙素原 C-反应蛋白

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Design and validation of the THMC China-Mock-Up test on buffer material for HLW disposal

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Journal of Rock Mechanics and Geotechnical Engineering 2014年第2期6卷 119-125页

作者： Yuemiao Liu Like Ma Dan Ke Shengfei Cao Jingli Xie xingguang Zhao Liang Chena Panpan zhang Beijing Research Institute of Uranium Geology CNNC Key Laboratory on Geological Disposal of High-level Radioactive Waste

According to the preliminary concept of the high-level radioactive waste （HLW） repository in China, a large-scale mock-up facility, named China-Mock-Up was constructed in the laboratory of Beijing Research Institute of Uranium Geology （BRIUG）. A heater, which simulates a container of radioactive waste, is placed inside the compacted Gaomiaozi （GMZ）-Na-bentonite blocks and pellets. Water inflow through the barrier from its outer surface is used to simulate the intake of groundwater. The numbers of water injection pipes, injection pressure and the insulation layer were determined based on the nu- merical modeling simulations. The current experimental data of the facility are herein analyzed. The experiment is intended to evaluate the thermo-hydro-mechano-chemical （THMC） processes occurring in the compacted bentonite-buffer during the early stage of HLW disposal and to provide a reliable database for numerical modeling and further investigation of engineered barrier system （EBS）, and the design of HLW repository.

关键词： High-level radioactive waste (HLW) Mock-Up test Numerical modeling Bentonite

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油气田开发用泡沫流体理论与技术新进展

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油田化学 2023年第3期40卷 562-570页

作者：许星光张鹏张旭张希文王超中国地质大学(武汉)资源学院湖北武汉430074 中国石油国际勘探开发有限公司北京100034 中国石油勘探开发研究院北京100083

随着油气藏类型和井筒结构愈加呈现出多样化和复杂性,要想实现复杂油气藏和复杂井况的油气资源经济有效动用,先进、高效、稳定的提高采收率技术和增产措施至关重要。泡沫因其密度小、黏度高、能有效保护油气层、调剖堵水能力强以及驱油... 详细信息

随着油气藏类型和井筒结构愈加呈现出多样化和复杂性,要想实现复杂油气藏和复杂井况的油气资源经济有效动用,先进、高效、稳定的提高采收率技术和增产措施至关重要。泡沫因其密度小、黏度高、能有效保护油气层、调剖堵水能力强以及驱油机理完善等性能,在钻完井、固井、储层保护、储层改造和提高采收率等领域都取得了较好的现场应用效果。本文在介绍泡沫相关理论的基础上,归纳总结了近年来泡沫流体在非常规油气藏开发各个领域的室内研究与矿场试验,分析了泡沫流体提高采收率和增产措施的优缺点,并展望其未来发展方向。

关键词：泡沫流体泡沫钻井液泡沫酸压裂调驱综述

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基于手机媒体的“化工原理”课程教学探索

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广东化工 2017年第20期44卷 168-169页

作者：王洁顾晓利陈慕华李小保姚建峰江华姚春才汪洋李力成冯艺张兴光张雄飞李月琴张小祥邵伟南京林业大学化学工程学院江苏南京210037

化工原理是化工及相关专业学生学习的一门重要的技术基础课,对学生就业和发展具有重要作用。本文针对传统化工原理教学中存在的问题,基于手机媒体的移动学习该课程能更好地满足学生对不同知识点的个性化学习。手机媒体不仅仅是化工原理... 详细信息

化工原理是化工及相关专业学生学习的一门重要的技术基础课,对学生就业和发展具有重要作用。本文针对传统化工原理教学中存在的问题,基于手机媒体的移动学习该课程能更好地满足学生对不同知识点的个性化学习。手机媒体不仅仅是化工原理课程移动学习资源的平台,还是师生沟通交流的新型工具,对师生亲密关系的建立有着积极作用。因此,手机媒体对化工原理课程教学效果起着至关重要的作用,应充分发挥其在教学过程中的正效用。

关键词：化工原理手机媒体移动学习教学改革

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KDM2B promotes IL-6 production and inflammatory responses through Brg1-mediated chromatin remodeling

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Cellular & Molecular Immunology 2020年第8期17卷 834-842页

作者： Qingqing Zhou Yunkai zhang Bo Wang Wenhui Zhou Yong Bi Wanwan Huai Xi Chen Yihan Chen Zhongmin Liu xingguang Liu Zhenzhen Zhan Institute of Heart Failure Shanghai East HospitalTongji University School of Medicine200120ShanghaiChina National Key Laboratory of Medical Immunology&Institute of Immunology Second Military Medical University200433ShanghaiChina Shanghai Fourth People’s Hospital Tongji University School of Medicine200081ShanghaiChina Institute of Immunology Zhejiang University School of Medicine310058HangzhouChina Key Laboratory of Arrhythmias of the Ministry of Education of China Shanghai East HospitalTongji University School of Medicine200120ShanghaiChina

IL-6 plays important and pleiotropic roles in infection and inflammatory diseases,and its production needs to be tightly ***,the epigenetic mechanism underlying Il6 gene transcription remains to be fully ***,we report that lysine-specific demethylase 2b(KDM2B),which demethylates H3K4me3 and H3K36me2,is required in macrophages and dendritic cells for the induction of IL-6 but not TNF-α,IL-1,and IFN-β.Compared to wild-type mice,KDM2B-deficient mice were more resistant to endotoxin shock and colitis,with a less severe inflammatory pathogenesis phenotype and decreased IL-6 production in ***2B selectively bound the Il6 promoter but did not alter histone demethylation;instead,KDM2B interacted with Brahma-related gene 1(Brg1),the core ATPase subunit of SWI/SNF chromatin remodeling complexes,to facilitate chromatin accessibility of the Il6 ***,KDM2B directly recruited RNA Polymerase II to further initiate and promote Il6 ***,our finding identifies a novel nonclassical function of KDM2B in gene-specific transcription initiation and enhancement of Il6 independent of its demethylase activity and adds new insight into the specific epigenetic modification mechanism of inflammatory immune responses.

关键词： KDM2B IL-6 Brg1 Chromatin remodeling Inflammation

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