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Puerarin@Chitosan composite for infected bone repair through mimicking the bio-functions of antimicrobial peptides

Bioactive Materials 2023年第3期21卷 520-530页

作者： Liping Ouyang Baohui Chen xingdan liu Donghui Wang Yang Li Yun Liao Kelvin W.K.Yeung Xuanyong liu State Key Laboratory of High Performance Ceramics and Superfine Microstructure Shanghai Institute of CeramicsChinese Academy of SciencesShanghai200050China Hongqiao International Institute of edicine Shanghai Jiao Tong University School of MedicineShanghai200336China Cixi Center of Biomaterials Surface Engineering Ningbo315300China Department of Computational Biology for Individualised Medicine Centre for Individualised Infection Medicine(CiiM)&TWINCOREJoint Ventures Between the Helmholtz-Centre for Infection Research(HZI)and the Hannover Medical School(MHH)HannoverGermany Shenzhen Key Laboratory for Innovative Technology in Orthopaedic Trauma Guangdong Engineering Technology Research Center for Orthopaedic Trauma RepairDepartment of Orthopaedics and TraumatologyThe University of Hong Kong Shenzhen HospitalShenzhenChina

It is important to eliminate lipopolysaccharide(LPS)along with killing bacteria in periprosthetic joint infection(PJI)therapy for promoting bone repair due to its effect to regulate macrophages response.Although natural antimicrobial peptides(AMPs)offer a good solution,the unknown toxicity,high cost and exogenetic immune response hamper their applications in clinic.In this work,we fabricated a nanowire-like composite material,named P@C,by combining chitosan and puerarin via solid-phase reaction,which can finely mimic the bio-functions of AMPs.Chitosan,serving as the bacteria membrane puncture agent,and puerarin,serving as the LPS target agent,synergistically destroy the bacterial membrane structure and inhibit its recovery,thus endowing P@C with good antibacterial property.In addition,P@C possesses good osteoimmunomodulation due to its ability of LPS elimination and macrophage differentiation modulation.The in vivo results show that P@C can inhibit the LPS induced bone destruction in the Escherichia coli infected rat.P@C exhibits superior bone regeneration in Escherichia coli infected rat due to the comprehensive functions of its superior antibacterial property,and its ability of LPS elimination and immunomodulation.P@C can well mimic the functions of AMPs,which provides a novel and effective method for treating the PJI in clinic.

关键词： Osteoimmunomodulation Antibacterial Chitosan Puerarin Lipopolysaccharide

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Hydroxyapatite composited PEEK with 3D porous surface enhances osteoblast differentiation through mediating NO by macrophage

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Regenerative Biomaterials 2022年第1期9卷 107-118页

作者： xingdan liu Liping Ouyang Lan Chen Yuqin Qiao Xiaohan Ma Guohua Xu Xuanyong liu State Key Laboratory of High Performance Ceramics and Superfine Microstructure Shanghai Institute of CeramicsChinese Academy of SciencesDingxi Road 1295Shanghai 200050China Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesYuquan Road 19Beijing 100049China Tongren Hospital Shanghai Jiao Tong University School of Medicine1111 Xianxia RoadShanghai 200336China School of Materials Science and Engineering&Henan Key Laboratory of Advanced Magnesium Alloy&Key Laboratory of Materials Processing and Mold Technology(Ministry of Education)Zhengzhou UniversityScience Avenue 100Zhengzhou 450001China Cixi Center of Biomaterials Surface Engineering Shanghai Institute of CeramicsChinese Academy of SciencesWenwei Road 345Ningbo 315300China Department of Orthopedic Surgery Spine CenterChangzheng HospitalNaval Medical UniversityNo.415 Fengyang RoadShanghai 200003China

The adverse immune response mediated by macrophages is one of the main factors that are prone to lead poor osseointegration of polyetheretherketone(PEEK)implants in clinic.Hence,endowing PEEK with immunomodulatory ability to avoid the adverse immune response becomes a promising strategy to promote bone repair.In this work,sulfonation and hydrothermal treatment were used to fabricate a 3D porous surface on PEEK and hydroxyapatite(HA)composited PEEK.The HA composited PEEK with 3D porous surface inhibited macrophages polarizing to M1 phenotype and downregulated inducible nitric oxide synthase protein expression,which led to a nitric oxide concentration reduction in culture medium of mouse bone marrow mesenchymal stem cells(mBMSCs)under coculture condition.The decrease of nitric oxide concentration could help to increase bone formation-related OSX and ALP genes expressions and decrease bone resorption-related MMP-9 and MMP-13 genes expressions via cAMP–PKA–RUNX2 pathway in mBMSCs.In summary,the HA composited PEEK with 3D porous surface has the potential to promote osteogenesis of PEEK through immunomodulation,which provides a promising strategy to improve the bone repair ability of PEEK.

关键词： polyetheretherketone three-dimensional porous hydroxyapatite osteoimmunology nitric oxide

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A new gain-of-function OsGS2/GRF4 allele generated by CRISPR/Cas9 genome editing increases rice grain size and yield

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The Crop Journal 2022年第4期10卷 1207-1212页

作者： Wenshu Wang Weipeng Wang Yanlin Pan Chao Tan Hongjing Li Ya Chen xingdan liu Jing Wei Nian Xu Yu Han Han Gu Rongjian Ye Qi Ding Chonglie Ma Life Science and Technology Center China National Seed Group Co.Ltd.Wuhan 430000HubeiChina State Key Laboratory of Crop Breeding Technology Innovation and Integration China National Seed Group Co.Ltd.Wuhan 430000HubeiChina

Grain size is one of the most important factors affecting rice grain quality and yield,and attracts great attention from molecular biologists and breeders.In this study,we engineered a CRISPR/Cas9 system targeting the miR396 recognition site of the rice GS2 gene,which encodes growth-regulating factor 4(OsGRF4)and regulates multiple agronomic traits including grain size,grain quality,nitrogen use efficiency,abiotic stress response,and seed shattering.In contrast to most previous genome editing efforts in which indel mutations were chosen to obtain null mutants,a mutant named GS2^(E) carrying an in-frame 6-bp deletion and 1-bp substitution within the miR396-targeted sequence was identified.GS2^(E) plants showed increased expression of GS2 in consistent with impaired repression by miR396.As expected,the gain-of-function GS2^(E) mutant exhibited multiple beneficial traits including increased grain size and yield and bigger grain length/width ratio.Thousand grain weight and grain yield per plant of GS2^(E) plants were increased by 23.5%and 10.4%,respectively.These improved traits were passed to hybrids in a semidominant way,suggesting that the new GS2^(E) allele has great potential in rice improvement.Taken together,we report new GS2 germplasm and describe a novel gene-editing strategy that can be widely employed to improve grain size and yield in rice.This trait-improvement strategy could be applied to other genes containing miRNA target sites,in particular the conserved miR396-GRF/GIF module that governs plant growth,development and environmental response.

关键词： Genome editing GS2/GRF4 Grain size Yield Rice

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Synergistic effects of immunoregulation and osteoinduction of ds-block elements on titanium surface

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Bioactive Materials 2021年第1期6卷 191-207页

作者： Lan Chen Donghui Wang Jiajun Qiu Xianming Zhang xingdan liu Yuqin Qiao Xuanyong liu State Key Laboratory of High Performance Ceramics and Superfine Microstructure Shanghai Institute of CeramicsChinese Academy of SciencesShanghai200050China Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijing100049China School of Materials Science and Engineering Hebei University of TechnologyTianjin300130China School of Chemistry and Materials Science Hangzhou Institute for Advanced StudyUniversity of Chinese Academy of SciencesHangzhou310024China

Ds-block elements have been gaining increasing attention in the field of biomaterials modification,owing to their excellent biological properties,such as antibiosis,osteogenesis,etc.However,their function mechanisms are not well understood and conflicting conclusions were drawn by previous studies on this issue,which are mainly resulted from the inconsistent experimental conditions.In this work,three most widely used ds-block elements,copper,zinc,and silver were introduced on titanium substrate by plasma immersion ion implantation method to investigate the rule of ds-block elements in the immune responses.Results showed that the implanted samples could decrease the inflammatory responses compared with Ti sample.The trend of anti-inflammatory effects of macrophages on samples was in correlation with cellular ROS levels,which was induced by the implanted biomaterials and positively correlated with the number of valence electrons of ds-block elements.The co-culture experiments of macrophages and bone marrow mesenchymal stem cells showed that these two kinds of cells could enhance the anti-inflammation and osteogenesis of samples by the paracrine manner of PGE2.In general,in their steady states on titanium substrate(Cu2+,Zn2+,Ag),the ds-block elements with more valence electrons exhibit better anti-inflammatory and osteogenic effects.Moreover,molecular biology experiments indicate that the PGE2-related signaling pathway may contribute to the desired immunoregulation and osteoinduction capability of ds-block elements.These findings suggest a correlation between the number of valence electrons of ds-block elements and the relevant biological responses,which provides new insight into the selection of implanted ions and surface design of biomaterials.

关键词： Ds-block element Valence electron Immune response Osteoimmunology Molecular mechanism

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Strontium ranelate incorporated 3D porous sulfonated PEEK simulating MC3T3-E1 cell differentiation

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Regenerative Biomaterials 2021年第1期8卷 43-51页

作者： Yingxiao Sunt xingdan liu Ji Tan Dan Lv Wengang Song Rui Su Ling Li Xuanyong liu Liping Ouyang Yun Liao Department of Pharmacy Tongren HospitalShanghai Jiao Tong University School of MedicineShanghai 200336China State Key Laboratory of High Performance Ceramics and Superfine Microstructure Shanghai Institute of CeramicsChinese Academy of SciencesChangning DistrictShanghai 200050China Graduate School of Beihua University Beihua UniversityFengman District Jilin 132013China

Polyetheretherketone(PEEK)has been used as an implant material because it has similar mechani-cal properties to natural bone.However,inferior osseointegration and bioinertness hamper the clinical application of PEEK.In this study,the surfaces of sulfonated three-dimensional(3D)PEEK porous structures were loaded with different concentrations of strontium ranelate,a compound commonly used in the treatment or prevention of osteoporosis by promoting bone formation and inhibiting bone resorption.Field-emission scanning electron microscopy was used to characterize the topography of the structures,elemental carbon,oxygen and strontium contents were mea-sured by X-ray photoelectron spectroscopy,and surface zeta potentials and water-contact angle were also measured.The results indicated that strontium ranelate was successfully loaded onto the 3D porous structures.In vitro cellular results showed that strontium ranelate-treated sulfonated PEEK(SP-SR)strengthened the adhesion of MC3T3-E1 cells.The activity of alkaline phosphatase,collagen secretion and extracellular matrix mineralization deposition of MC3T3-E1 cells were also improved on the surface of SP-SR.These results indicate that SP-SR could serve a new implant candidate for surgical treatment.

关键词： polyetheretherketone sufonation strontium ranelate osseointegr ation

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基于LCL滤波器的逆变器并网有源阻尼控制策略

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信息技术与信息化 2024年第3期 148-152页

作者：焦岳超刘幸丹巫付专焦健航江腾龙中原工学院电子信息学院河南郑州450000

目前LCL滤波器的逆变器广泛应用于并网过程中,由于本身是三阶欠阻尼系统,常常出现谐振尖峰,进而导致系统不稳定。对此,提出一种基于带通滤波器单反馈有源阻尼策略。首先,选取合适的LCL滤波器参数实现并网条件。其次,从幅值特性和相位裕... 详细信息

目前LCL滤波器的逆变器广泛应用于并网过程中,由于本身是三阶欠阻尼系统,常常出现谐振尖峰,进而导致系统不稳定。对此,提出一种基于带通滤波器单反馈有源阻尼策略。首先,选取合适的LCL滤波器参数实现并网条件。其次,从幅值特性和相位裕度的角度出发,采用极点配置法对带通滤波器进行参数设计并设置反馈并网高频电流。最后,通过MATLAB/Simlink仿真验证带通滤波器有源阻尼方法的可行性和有效性。

关键词： LCL型滤波器单相逆变器有源阻尼带通滤波器极点配置法

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热处理对半固态挤压SiC_p/2024复合材料的影响

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特种铸造及有色合金 2016年第4期36卷 422-424页

作者：马郁柏阎峰云刘兴丹赵红娟兰州理工大学省部共建有色金属先进加工与再利用国家重点实验室兰州理工大学有色金属合金及加工教育部重点实验室

采用扫描电镜(SEM)、X射线衍射仪(XRD)、电子探针等研究了固溶处理对半固态挤压SiC_p/2024复合材料的组织和性能的影响。结果表明,SiCp/2024复合材料经过500℃×12h固溶和170℃×16h时效处理后,抗拉强度达到了478MPa,硬度(HV)为150,而... 详细信息

采用扫描电镜(SEM)、X射线衍射仪(XRD)、电子探针等研究了固溶处理对半固态挤压SiC_p/2024复合材料的组织和性能的影响。结果表明,SiCp/2024复合材料经过500℃×12h固溶和170℃×16h时效处理后,抗拉强度达到了478MPa,硬度(HV)为150,而在未热处理之前材料硬度(HV)仅为86,抗拉强度也只有350 MPa。

关键词： SiCp/2024复合材料固溶温度时效温度力学性能

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SiCp/Al复合材料表面电镀Ni-P合金的工艺研究

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材料科学与工艺 2017年第1期25卷 35-43页

作者：赵红娟阎峰云刘兴丹陈体军马郁柏省部共建有色金属先进加工与再利用国家重点实验室(兰州理工大学) 兰州730050 有色金属合金及加工教育部重点实验室(兰州理工大学) 兰州730050

在高体积分数SiCp/Al复合材料表面镀覆一层Ni-P合金可有效改善其可焊性.为在体积分数60%的SiCp/Al复合材料表面电镀一层Ni-P合金,采用正交试验对电镀工艺参数进行了优化,研究了电镀前预处理工艺,并考察了预处理对电镀层的影响.由正交试... 详细信息

在高体积分数SiCp/Al复合材料表面镀覆一层Ni-P合金可有效改善其可焊性.为在体积分数60%的SiCp/Al复合材料表面电镀一层Ni-P合金,采用正交试验对电镀工艺参数进行了优化,研究了电镀前预处理工艺,并考察了预处理对电镀层的影响.由正交试验获得了最佳硬度和最佳表面质量的工艺参数;采用SEM、EDS、X射线衍射仪和显微硬度计等对镀层进行表征.研究结果表明:P含量通过影响Ni-P的晶体结构,进而影响其性能,随着P含量(9.38%~15.4%)的增加,Ni-P的非晶态结构越明显,硬度在P含量11.4%时达到最大值723.3 HV;与SiCp/Al表面SiC相上的沉积相比,电沉积Ni-P在Al相上的初始沉积迅速,且长大速度快,导致镀层微观表面凹凸不平.经165℃活化热处理及化学镀18 min后,再电镀40 min,获得的镀层微观表面平整、厚12.90~14.79μm.说明经过优化工艺参数和预处理,可制备表面平整且结合良好的Ni-P电镀层.

关键词： SiCp/Al复合材料电镀正交试验 Ni-P镀层预处理

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烧结温度对SiC_p/Al复合材料组织与性能的影响

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粉末冶金材料科学与工程 2017年第1期22卷 20-25页

作者：刘兴丹阎峰云赵红娟陈体军马郁柏兰州理工大学省部共建有色金属先进加工与再利用国家重点实验室兰州730050 兰州理工大学有色金属合金及加工教育部重点实验室兰州730050

借助于直热法粉末触变成形,通过控制加电方式,压制成形并烧结制备SiC_p/Al复合材料,并对复合材料进行微观组织分析及热物理性能与力学性能测试,研究烧结温度对复合材料微观组织、热膨胀系数、热导率及抗弯强度的影响。结果表明:随烧结... 详细信息

借助于直热法粉末触变成形,通过控制加电方式,压制成形并烧结制备SiC_p/Al复合材料,并对复合材料进行微观组织分析及热物理性能与力学性能测试,研究烧结温度对复合材料微观组织、热膨胀系数、热导率及抗弯强度的影响。结果表明:随烧结温度升高,复合材料内气孔减少,热膨胀系数先减小后增大,热导率逐渐增大,抗弯强度先增大后减小。最佳烧结温度为600℃,此温度下制备的含SiC_p体积分数60%的SiC_p/Al复合材料中,SiC_p颗粒分布均匀,材料组织致密;室温至250℃平均热膨胀系数小于5.0×10-6℃-1,其室温热导率为165W/(m·℃),密度为3.01 g/cm3,复合材料的抗弯强度为340 MPa。

关键词：烧结温度触变成形 Si Cp/Al复合材料热膨胀系数热导率抗弯强度

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Experimental investigation on heat transfer enhancement of mist/air impingement jet

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Science China(Technological Sciences) 2013年第10期56卷 2456-2464页

作者： TAN XiaoMing ZHANG JingZhou liu Bo ZHU xingdan Jiangsu Province Key Laboratory of Aerospace Power Systems College of Energy and Power EngineeringNanjing University of Aeronautics and Astronautics

The heat transfer performance of a mist/air jet impingement on a constant-heat flux surface was experimentally investigated.Two objectives were outlined in the current study.The first objective is to assess the effects of mist/air volumetric flow rate ratio,impinging mode and heat flux on the heat transfer characteristics of free mist/air jet impingement.The second objective is to assess the effect of swirl flow induced by the spinning grinding wheel on the mist/air jet impingement,simulating the heat transfer process on a grinding work-piece surface subjected to the mist/air jet impingement.The results show that the addition of dilute water droplets to air flow results in significant heat transfer enhancement.Once the mist/air ratio is increased to a certain value,the increase of heat transfer with the mist/air ratio becomes slow.For a given mist/air ratio,as the increase of heat flux,the contribution of droplet evaporation to the overall heat transfer is weakened relatively,resulting in a decrease of heat transfer enhancement in comparison to the lower heat flux case.The heat transfer coefficient in the stagnation region for the oblique jet is much lower than the normal mist/air jet impingement,while in the region away from the stagnation,the local heat transfer coefficient for the oblique jet is higher than the normal jet.As regards as the mist/air jet impingement in the vicinity of grinding zone is concerned,when the jet impinging direction is consistent with the rotating direction of rotating disk,the swirl flow induced by the rotating disk could entrain more droplets to enter the jet impinging stagnation zone,which is beneficial to convective heat transfer enhancement.Furthermore,as the rotational speed of disk increases,the temperature deceases in impinging jet stagnation zone.

关键词：冲击射流传热实验空气比烟雾强化局部传热系数射流冲击液滴蒸发

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