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Depletion of anti-CD47mAb in plasma by genetically modified cells for pre-transfusion testing

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Genes & Diseases 2024年第5期11卷 102-104页

作者： Fei Wang Wenting Wang Xiaoshuang Wu Zhixin Liu Yafen Wang Rong Zhang Shunli Gu Qunxing An Yaozhen Chen xingbin hu Department of Transfusion Medicine Xijing HospitalFourth Military Medical UniversityXi’anShaanxi 710032China Xi’an Medical University Xi’anShaanxi 710021China

Allogeneic red blood cell(RBC)transfusion is commonly performed in medical practice because of its efficacy and low-risk ***,pre-transfusion tests are susceptible to monoclonal antibody(mAb)interference.1 Currently,mAb therapies are being developed to treat many diseases,such as ***,certain mAbs,such as anti-CD38mAb and anti-CD47mAb,can bind to RBC membranes;this binding interferes with pre-transfusion tests.2 CD47 has gained considerable attention in recent years because of its potential as a therapeutic target for hematologic malignancies and solid tumors.3 The binding of anti-CD47mAb to RBCs may lead to false-positive results in pan-agglutination tests and cause delays and risks in establishing compatible RBCs for transfusion.

关键词： CD47 transfusion diseases

A novel coumarin derivative DBH2 inhibits proliferation and induces apoptosis of chronic myeloid leukemia cells

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Genes & Diseases 2023年第2期10卷 596-607页

作者： Jiajia Xin huijie Zhang Dandan Yin Ning An Yaozhen Chen Jinmei Xu Jing Zhang Zhixin Liu Yongsheng Liu Wen Yin Mingkai Li xingbin hu Department of Blood Transfusion Xijing HospitalAir Force Medical UniversityXi’anShaanxi 710032China Key Laboratory of Biochemistry and Molecular Pharmacology of Chongqing Chongqing Medical UniversityChongqing 400016China Department of Hematology Tangdu HospitalAir Force Medical UniversityXi’anShaanxi 710038China Department of Pharmacology School of PharmacyAir Force Medical UniversityXi’anShaanxi 710032China

With the development of tyrosine kinase inhibitor(TKI)resistance,finding the novel effective chemotherapeutic agent is of seminal importance for chronic myelogenous leukemia(CML)*** study aims to find the effective anti-leukemic candidates and investigate the possible underlying *** synthesized the novel coumarin derivatives and evaluated their anti-leukemic *** viability assay revealed that compound DBH2 exhibited the potent inhibitory activity on the proliferation of CML K562 cells and TKI resistant K562 *** observation and flow cytometry confirmed that DBH2 could selectively induce cell apoptosis and cell cycle arrest at G2/M phase of the K562 cells,which was further confirmed on the bone marrow cells from CML transgenic model mice and CD34+bone marrow leukemic cells from CML *** of DBH2 in combination with imatinib could prolong the survival rate of SCL-tTA-BCR/ABL transgenic model mice *** RT-PCR revealed that DBH2 inhibited the expression of STAT3 and STAT5 in K562 cells,and caspase-3 knockout alleviated the DBH2 induced ***,DBH2 could induce the expression of PARP1 and ROCK1 in K562 cells,which may play the important role in caspase-dependent *** results concluded that coumarin derivative DBH2 serves as a promising candidate for the CML treatment,especially in the combination with imatinib for the TKI resistant CML,and STAT/caspase-3 pathway was involved in the molecular mechanism of anti-leukemic activity of DBH2.

关键词： Apoptosis Caspase Chronic myeloid leukemia Coumarin STAT

Glutamine metabolic microenvironment drives M2 macrophage polarization tomediate trastuzumab resistance in HER2-positive gastric cancer

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Cancer Communications 2023年第8期43卷 909-937页

作者： xingbin hu Zhenfeng Ma Beibei Xu Shulong Li Zhiqi Yao Bishan Liang Jiao Wang Wangjun Liao Li Lin Chunling Wang Siting Zheng Qijing Wu Qiong huang Le Yu Fenghua Wang Min Shi Department of Oncology Nanfang HospitalSouthern Medical UniversityGuangzhouGuangdongP.R.China School of Biomedical Engineering Southern Medical UniversityGuangzhouGuangdongP.R.China School of Pharmaceutical Sciences Southern Medical UniversityGuangzhouGuangdongP.R.China Department of Medical Oncology Sun Yat-sen University Cancer CenterState Key Laboratory of Oncology in South ChinaCollaborative Innovation Center for Cancer MedicineGuangzhouGuangdongP.R.China

Background:Trastuzumab is a first-line targeted therapy for human epidermal growth factor receptor-2(HER2)-positive gastric ***,the inevitable occurrence of acquired trastuzumab resistance limits the drug benefit,and there is currently no effective reversal *** researches on the mechanism of trastuzumab resistance mainly focused on tumor cells themselves,while the understanding of the mechanisms of environment-mediated drug resistance is relatively *** study aimed to further explore the mechanisms of trastuzumab resistance to identify strategies to promote survival in these ***:Trastuzumab-sensitive and trastuzumab-resistant HER2-positive tumor tissues and cells were collected for transcriptome *** were used to analyze cell subtypes,metabolic pathways,and molecular signaling *** in microenvironmental indicators(such as macrophage,angiogenesis,and metabolism)were verified by immunofluorescence(IF)and immunohistochemical(IHC)***,a multi-scale agent-based model(ABM)was *** effects of combination treatment were further validated in nude mice to verify these effects predicted by the ***:Based on transcriptome sequencing,molecular biology,and in vivo experiments,we found that the level of glutamine metabolism in trastuzumabresistant HER2-positive cells was increased,and glutaminase 1(GLS1)was significantly ***,tumor-derived GLS1 microvesicles drove M2macrophage ***,angiogenesis promoted trastuzumab *** showed high glutamine metabolism,M2 macrophage polarization,and angiogenesis in trastuzumab-resistant HER2-positive tumor tissues from patients and ***,the cell division cycle 42(CDC42)promoted GLS1 expression in tumor cells by activating nuclear factor kappa-B(NF-κB)p65 and drove GLS1microvesicle secretion through IQmotif-containing GTPase-activating protein 1(IQGAP1).Based on the ABM and

关键词： Gastric cancer glutamine metabolism macrophage mathematical model microvesicles trastuzumab

Inhibitory effects of apogossypolone on subcutaneous implants of human LNCaP prostatic carcinoma cells

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The Chinese-German Journal of Clinical Oncology 2012年第1期11卷 33-36页

作者： Yaozhen Chen Haishan Chen Chen Chen Xiaofeng huang Shijie Mu Mengyao Zhang xingbin hu Qunxing An Xianqing Zhang Department of Blood Transfusion Xijing Hospital The Fourth Military Medical University Xi＇an 710032 China Department of Blood Transfusion 210 Hospital of PLA Dalian 116000 China Central laboratory The Fourth Military Medical University Xi＇an 710032 China Department of Chemistry School of Pharmacy The Fourth Military Medical University Xi＇an 710032 China

Objective:The aim of this study was to investigate the inhibitory effect of apogossypolone (ApoG2) on subcutaneous implants of human LNCaP prostatic carcinoma cells, and explore its mechanism. Methods:To establish human LNCaP prostatic carcinoma cell line subcutaneous xenograft models and observe the inhibitory effect of ApoG2 on the tumor model. Immunohistochemistry was employed to observe the expression of Bcl-2, PCNA, CD31, caspase-3 and-8 in tumor tissues. The microvessel density was calculated. Results:ApoG2 could obviously inhibit the growth of subcutaneous prostatic carcinoma implant. ApoG2 decreased the expression of PCNA and CD31, and increased the expression of caspases-3,-8 in tumor tissues. Conclusion:ApoG2 has an inhibitory effect on prostatic carcinoma implants.

关键词： apogossypolone (ApoG2) prostate cancer LNCaP human prostatic carcinoma cell line transplantation

基于系统动力学的汽车产业技术创新能力影响因素研究

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工业技术经济 2017年第2期36卷 50-56页

作者：李煜华荣爽胡兴宾哈尔滨理工大学哈尔滨150040

本文在分析汽车产业技术创新能力影响因素的基础上,构建汽车产业技术创新能力影响因素的系统动力学模型并进行模拟仿真,对影响汽车产业技术创新能力的各因素及其关系进行系统分析。仿真结果表明:增加要素的投入,尤其是增加企业家因素、... 详细信息

本文在分析汽车产业技术创新能力影响因素的基础上,构建汽车产业技术创新能力影响因素的系统动力学模型并进行模拟仿真,对影响汽车产业技术创新能力的各因素及其关系进行系统分析。仿真结果表明:增加要素的投入,尤其是增加企业家因素、产学研合作强度及外商投资力度对自主品牌汽车销量具有显著的正向影响;增加企业R&D和人力资源的投入不能给新产品销量带来显著影响;政府的支持有时会增加企业的创新成本。研究结果可为汽车产业进行技术创新提供决策依据。

关键词：技术创新能力系统动力学汽车产业自主品牌产学研合作

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生物医用高分子材料在医疗中的应用

中国医院建筑与装备 2008年第1期9卷 30-35页

作者：章俊胡兴斌李雄解放军第91中心医院工程科 454003 武汉大学化学院 430072

本文论述了生物医用高分子材料的发展概况,重点介绍了硅橡胶、聚氯乙烯、聚氨酯等生物医用高分子材料的特性及其在医疗器械中的应用。

关键词：高分子材料医疗器械应用

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小肠移植患者围术期成分输血治疗

中国输血杂志 2014年第11期27卷 1163-1164页

作者：夏爱军胡兴斌安群星陈晨南敏第四军医大学西京医院输血科陕西西安710032

目的探讨小肠移植围术期的输血策略及其特点。方法回顾性分析10例小肠移植术受者的临床输血资料与检测结果。结果 10例小肠移植术受者围术期输血量RBC平均为16.9 U,FFP平均用量为2 847 m L,未输注血小板及冷沉淀。结论尽管小肠移植围术... 详细信息

目的探讨小肠移植围术期的输血策略及其特点。方法回顾性分析10例小肠移植术受者的临床输血资料与检测结果。结果 10例小肠移植术受者围术期输血量RBC平均为16.9 U,FFP平均用量为2 847 m L,未输注血小板及冷沉淀。结论尽管小肠移植围术期血液成分的用量有很大个体差异,但仍属于大量用血。应做好术前、术中及术后的各项检验监测,适时地提供不同品种的血液成分进行输注,降低围术期输血风险。

关键词：小肠移植成分输血围手术期

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血小板诱导肝再生的研究进展

中国输血杂志 2018年第10期31卷 1205-1208页

作者：曾宪飞胡兴斌武警陕西省总队医院输血科陕西西安710054 空军军医大学西京医院输血科

肝脏能通过强大的再生能力进行自我修复。研究表明血小板在肝再生过程中发挥了重要作用,可以通过释放促有丝分裂因子,与肝窦内皮细胞、肝Kupffer细胞相互作用等多种机制参与肝再生。这种新发现的血小板功能为促进移植肝和肝部分切除后... 详细信息

肝脏能通过强大的再生能力进行自我修复。研究表明血小板在肝再生过程中发挥了重要作用,可以通过释放促有丝分裂因子,与肝窦内皮细胞、肝Kupffer细胞相互作用等多种机制参与肝再生。这种新发现的血小板功能为促进移植肝和肝部分切除后肝脏再生、急慢性肝损伤治疗等提供了新思路。然而,由于高血小板的促血栓形成、易激发系统性炎症反应、促肿瘤生长等效应,通过提高循环血小板数量或功能从而促进肝再生的治疗方法是否应用于临床,仍然需要深入研究。

关键词：血小板肝脏肝再生

巨噬细胞与血小板体外抑制耐甲氧西林金黄色葡萄球菌的增殖

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细胞与分子免疫学杂志 2018年第9期34卷 782-786页

作者：顾顺利刘二雄徐金梅胡兴斌尹文空军军医大学第一附属医院输血科陕西西安710032 空军军医大学基础医学院微生物学教研室陕西西安710032

目的体外研究巨噬细胞与去白细胞单采血小板抑制耐甲氧西林金黄色葡萄球菌(MRSA)增殖的效率,并初步探讨其作用机制。方法将4×108个/mL MRSA接种至LB培养体系,分为血小板组(每升加入2×10^(11)个去白细胞的单采血小板)、巨噬细胞组(每... 详细信息

目的体外研究巨噬细胞与去白细胞单采血小板抑制耐甲氧西林金黄色葡萄球菌(MRSA)增殖的效率,并初步探讨其作用机制。方法将4×108个/mL MRSA接种至LB培养体系,分为血小板组(每升加入2×10^(11)个去白细胞的单采血小板)、巨噬细胞组(每升加入1×10~9个体外诱导培养的巨噬细胞)、对照组(去白细胞单采血小板和巨噬细胞均不加)。培养不同时间后,检测两组菌液的吸光度值以反映细菌的增殖情况,并在培养2 h时采集菌液,倍比稀释后涂板计数细菌数量;流式细胞术检测血小板对MRSA的捕获,ELISA检测对照组和血小板组中的血小板因子4(PF4)的含量。结果与对照组相比,巨噬细胞组和血小板组均可抑制MRSA增殖,且血小板组的作用更加显著。结论血小板可以在体外抑制MRSA增殖,其抑制效果优于巨噬细胞。

关键词：巨噬细胞去白细胞单采血小板耐甲氧西林金黄色葡萄球菌