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Chinese Chemical Letters 2007年第4期18卷 441-444页

作者： Jian Quan Lu Ming Bo Xu xing Wang zhou Jin Guang Xu Qing Tao Hubei Key Laboratory of Bioanalytical Technique Hubei Normal University Huangshi 435002 China Department of Chemistry Central China Normal University Wuhan 430079 China

An ss-DNA gold chip was prepared based on self-assembly of the thiol-derivatized oligonucleotide, and used for the determination of single-stranded binding protein （SSB） by surface plasmon resonance microscopy （SPR）. The experiment results sh.wed that SSB binds ss-DNA with high specificity, and relative signal of SPR response is proportional to the concentration of SSB in the range of 0.1-100 ng/mL with a detection limit （S/N = 3） of 0.07 ng/mL.

关键词： Detection Single-stranded DNA binding protein ss-DNA Surface plasmon resonance microscopy

Design, synthesis and immunomodulating activity of C-pseudonucleosides containing thiazolidin-4-one and phenyl connected by acetamide bond

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Chinese Chemical Letters 2016年第6期27卷 938-942页

作者： Hua Chen sh.n-Kai xing Fang Gao Na Li Xiao-Liu Li Ming Meng Key Laboratory of Chemical Biology of Hebei Province College of Chemistry and Environmental Science Hebei University Medical School Hebei University

Several novel C-pseudonucleosides containing thiazolidin-4-one and phenyl connected by acetamide bond were rationally designed and easily synthesized at room temperature by using the unprotected sugar aldehyde as the starting material. The effects of the compounds on Con A-induced T cell proliferation were evaluated at five concentrations of 5, 10, 25, 50, and 100 mmol/L Interestingly,compounds 7a and 8a（n = 2, R = H） exhibited immunostimulating activities, while compounds 5a, 6a（n = 1, R = H） and 7b, 8b（n = 2, R = CH3） sh.wed immunosuppressive activities. Another two compounds 5 b and 6b（n = 1, R = CH3） had no immunomodulating activities. These initial biological results suggested that subtle structural changes to the phenyl and acetamide bond of C-pseudonucleosides could have a significant effect on T cell proliferation bias, although it was difficult to formulate a rigorous structureactivity relationsh.p based on the observed activities.

关键词： Immunomodulator C-Pseudonucleosides Thiazolidin-4-one Acetamide bond SAR analysis

A novel rat model of seminal vesiculitis

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Asian Journal of Andrology 2019年第4期21卷 360-364页

作者： Peng zh.ng Xiao-Long Wang zh.ng-Hua Yang Xin-Jun Su xing-Huan Wang Department of Urology Zhongnan Hospital Wuhan University Wuhan 430071 China Department of Urology Research Lab/LIFE-Zentrum University of Munich (LMU) Munchen 81377 Germany

We aimed to establish.a novel rat model of seminal vesiculitis that would provide an effective approach to investigate the pathogenesis of this disease in the future. Eight male rats received the same operation, during which the root of one of the two seminal vesicles was partly ligatured with sutures and the other vesicle was left in tact. The samples of seminal vesicles were harvested on the 8th day followi ng the operation. Hematoxylin and eosi n and Mass on's trichrome stains were used to observe the histopathology and the presenee of fibrous tissue in seminal vesicles, respectively. Immunoblotting and immunohistochemistry were applied to determine the tumor n ecrosis factor-alpha and cyclooxygenase-2 levels in semi nal vesicle tissues. Real-time fluoresce nee qua ntitative polymerase chain reaction was performed to measure the gene expression levels of proinflammatory cytokines. H2O2 levels in the seminal plasma from the seminal vesicle were also measured. Hematoxylin and eosin staining suggested that there was inflammatory cell in filtration into the semi nal vesicles treated by partial root ligati on. The tumor n ecrosis factor-alpha and cyclooxygenase-2 proteins were significantly upregulated in the treated seminal vesicles. The tumor necrosis factor-alpha, cyclooxygenase, interleukin 6, and inducible nitric oxide synthase mRNA expression levels were also upregulated in the treated seminal vesicles. The H2O2 levels in the seminal plasma from seminal vesicles with partial root ligation were significantly elevated compared with those from vesicle left intact. In conclusion, partially ligating the root of the seminal vesicle via sutures in rats is an effective method to establish.a seminal vesiculitis rat model.

关键词： cyclooxygenase-2 rat model seminal vesiculitis tumor necrosis factor

Bronchoscopy for diagnosis of COVID-19 with respiratory failure:A case report

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World Journal of Clinical Cases 2021年第5期9卷 1132-1138页

作者： Qing-Yun Chen Yu-sh.ng He Kai Liu Jing Cao Yong-xing Chen Department of Respiratory and Critical Care Medicine Hainan General HospitalHaikou 570311Hainan ProvinceChina Department of Internal Medicine Hainan Province's Fucheng Drug Rehabilitation CentreHaikou 570311Hainan ProvinceChina Infection Department Hainan General HospitalHaikou 570311Hainan ProvinceChina

BACKGROUND Although the imaging features of coronavirus disease 2019(COVID-19)are starting to be well determined,what actually occurs within the bronchi is poorly ***,we report the processes and findings of bronchoscopy in a patient with COVID-19 accompanied by respiratory *** SUMMARY A 65-year-old male patient was admitted to the Hainan General Hospital on February 3,2020 for fever and sh.rtness of breath for 13 d that worsened for the last 2 *** severe acute respiratory syndrome coronavirus 2 nucleic acid test was *** blood examination on February 28 sh.wed a white blood cell count of 11.02×109/L,86.9%of neutrophils,6.4%of lymphocytes,absolute lymphocyte count of 0.71×109/L,procalcitonin of 2.260 ng/mL,and C-reactive protein of 142.61 mg/*** saturation was 46%at baseline and turned to 94%after *** patient underwent video *** tracheal cartilage ring was clear,and no deformity was found in the *** trachea and bilateral bronchi were patent,while the mucosa was with slight hyperemia;no neoplasm or ulcer was *** amounts of white gelatinous secretions were found in the dorsal segment of the left inferior lobe,and the bronchial lumen was patent after sputum *** right inferior lobe was found with hyperemia and mucosal erosion,with white gelatinous secretion *** patient’s condition did not improve after the application of therapeutic *** For patients with COVID-19 and respiratory failure,bronchoscopy can be performed under mechanical ventilation to clarify the airway *** sh.uld be worn during the *** the risk of infection,it is not necessary to perform bronchoscopy in the mild to moderate COVID-19 patients.

关键词： COVID-19 Respiratory failure Bronchoscopy Diagnosis Treatment Case report

Clinicopathological factors associated with pathological upgrading from biopsy to prostatectomy in patients with ISUP grade group≤2 prostate cancer

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Asian Journal of Andrology 2022年第5期24卷 487-493页

作者： xing Li zhi-Xian Wang Yun-Peng zhu Jing Wang Yi-sh.ng Yin Xiao-Yong Zeng Department of Urology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China

We performed this study to investigate pathological upgrading from biopsy to prostatectomy and clinicopathological factors associated with grade group(GG)upgrading in patients with International Society of Urological Pathology(ISUP)GG 1 and 2 prostate cancer(PCa)in a Chinese *** included patients diagnosed with PCa with ISUP GG 1 and 2 at biopsy,who underwent RP at our ***-and postoperative clinical variables were *** and multivariate logistic regression analyses were conducted to identify independent factors associated with GG *** in GG upgraded group had higher total prostatespecific antigen(tPSA;median:14.43 ng ml^(−1) vs 10.52 ng ml^(−1),P=0.001)and PSA density(PSAD;median:0.45 ng ml^(−2) vs 0.27 ng ml^(−2),PngReporting and Data System(PI-RADS)score>3(86.4%vs 67.9%,Png after RP than those with GG 2(71 vs 54,P=0.016).Independent preoperative factors predicting GG upgrading were PI-RADS score>3(odds ratio[OR]:2.471,95%confidence interval[CI]:1.132–5.393;P=0.023),higher PSAD(P=0.001),and GG in biopsy(OR:0.241,95%CI:0.123–0.471;P3,and PNI were independent factors of GG *** factors sh.uld be considered for patients with ISUP grade≤2 PCa.

关键词： biopsy grade group prostate cancer radical prostatectomy upgrading

A Chromosome-Level Genome Assembly of Garlic (Allium sativum) Provides Insights into Genome Evolution and Allicin Biosynthesis

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Molecular Plant 2020年第9期13卷 1328-1339页

作者： Xiudong Sun Siyuan zhu Ningyang Li Yi Cheng Jing zhao Xuguang Qiao Li Lu sh.qi Liu Yanzhou Wang Chan Liu Benping Li Wu Guo sh.ang Gao Zemao Yang Fu Li zh.ng Zeng Qing Tang Yupeng Pan Mengjiao Guan Jian zhao Xiaomi ng Lu Huanwe n Meng zhenlin Han Chun sh. ng Gao Wenkai Jiang xing zhao sh.lin Tian Jianguang Su zhihui Cheng Touming Liu sh.ndong Agricultural University Tai'an 271018China Institute of Bast Fiber Crops Chinese Academy of Agricultural SciencesChangsha 410205China Northwest A&F University Yangling 712100China Novogene Bioinformatics Institute Beijing 100083China School of Pharmaceutical Sciences Wuhan UniversityWuhan 430071China

Garlic,an economically important vegetable,spice,and medicinal crop,produces highly enlarged bulbs and unique organosulfur ***,we report a chromosome-level genome assembly for garlic,with a total size of approximately 16.24 Gb,as well as the annotation of 57561 predicted protein-coding genes,making garlic the first Allium species with a sequenced *** of this garlic genome assembly reveals a recent burst of transposable elements,explaining the substantial expansion of the garlic *** examined the evolution of certain genes associated with the biosynthesis of allicin and inulin neoseries-type fructans,and provided new insights into the biosynthesis of these two ***,a large-scale transcriptome was produced to characterize the expression patterns of garlic genes in different tissues and at various growth stages of enlarged *** reference genome and large-scale transcriptome data generated in this study provide valuable new resources for research on garlic biology and breeding.

关键词： garlic genome genome evolution allicin inulin neoseries-type fructans expression profiling

Field experiment using transient energy method to locate a single-phase to ground fault

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Global Energy Interconnection 2020年第6期3卷 585-594页

作者： Wei Xie Xuewen Wang Chen Fang Hengxu zh.ng Fang sh. Xiaodong xing Baicong Sun State Grid sh.nghai Municipal Electric Power Company No.1122 Yuanshen RoadShanghai Scan for more details 200122P.R.China State Grid Chongqing Electric Power Research Institute No.80 Huangshan RoadChongqing 401123P.R.China Key Laboratory of Power System Intelligent Dispatch and Control(Ministry of Education) Shandong UniversityNo.17923 Jingshi RoadJinan 250061P.R.China State Grid sh.nghai Electric Power Research Institute No.171 Handan RoadShanghai 200233P.R.China

Distribution networks in China and several other countries are predominantly neutral inefficiently grounding systems(NIGSs),and more than 80%of the faults in distribution networks are single-phase-to-ground(SPG)*** of the weak fault current and imperfect monitoring equipment configurations,methods used to determine the faulty line secti ons with SPG faults in NIGSs are in *** developme nt and application of distributi on-level phasor measurement units(PMUs)provide further comprehensive fault information for fault diagnosis in a distribution *** an SPG fault occurs,the transient energy of the faulted line section tends to be higher than the sum of the transient energies of other line *** this regard,transient energy-based fault location algorithms appear to be a promising *** this study,a field test plan was designed and implemented for a 10 kV distribution *** test results dem on strate the effective ness of the transient en ergy-based SPG locati on method in practical distributi on networks.

关键词： Sin gle-phase-to-gro und fault Fault location Neutral in efficiently groundi ng systems Distributi on n etwork Distribution-level PMU Transient energy.

卡纳琳-紫杉醇混悬剂的制备及其腹腔化疗的药代动力学研究

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中华胃肠外科杂志 2011年第12期14卷 973-976页

作者：蔡元坤张兴源上海市第五人民医院普通外科 200125

目的制备卡纳琳．紫杉醇混悬剂并研究其腹腔化疗的药代动力学。方法高效液相色谱法测定卡纳琳-紫杉醇的饱和吸附率。制备混悬剂。将34只Wistar大鼠随机分为实验组与对照组．分别腹腔注射卡纳琳-紫杉醇混悬液及单纯紫杉醇溶液．给药后7d... 详细信息

目的制备卡纳琳．紫杉醇混悬剂并研究其腹腔化疗的药代动力学。方法高效液相色谱法测定卡纳琳-紫杉醇的饱和吸附率。制备混悬剂。将34只Wistar大鼠随机分为实验组与对照组．分别腹腔注射卡纳琳-紫杉醇混悬液及单纯紫杉醇溶液．给药后7d内的不同时间点取各组大鼠血液、肠系膜淋巴结及腹腔洗液样本．采用高效液相-二级质谱联用法对紫杉醇浓度进行分析。结果每1ml卡纳琳可饱和吸附7mg紫杉醇。实验组血浆药物曲线下面积（AUC）为对照组的0．63倍；淋巴结药物AUC为对照组的0．73倍；腹水药物AUC为对照组的1．25倍。实验组血浆药物代谢半衰期（tm）为对照组的1．61倍；腹水药物t^1/2为对照组的0．88倍；淋巴结药物t^1/2为对照组的1．10倍。结论卡纳琳对紫杉醇吸附作用良好。以卡纳琳-紫杉醇混悬剂行腹腔化疗具有血药浓度低、腹腔药物浓度高、安全性高的特点，但淋巴靶向性及淋巴组织滞留作用不明显，其机制有待进一步研究。

关键词：卡纳琳卡纳琳-紫杉醇混悬剂腹腔化疗淋巴靶向化疗药代动力学

Concentrations and classification of HCHs and DDTs in soil from the lower reaches of the Jiulong River, China

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Frontiers of Environmental Science & Engineering 2012年第2期6卷 177-183页

作者： Jiaquan zh.ng sh.hua QI Xinli xing Lingzhi TAN Wei CHEN Ying HU Dan YAng Chenxi WU State Key Laboratory of Biogeology and Environmental Geology and School of Environmental StudiesChina University of GeosciencesWuhan 430074China School of Environmental Science and Engineering Huangshi Institute of TechnologyHuangshi 435003China

Soil is an important source to other environ- mental media and organisms for organochlorine pesticides （OCPs） bioaccumulation. Twenty-four representative sur- face soil samples were collected from the lower reaches of the Jiulong River, China, in 2009. The concentrations of hexachlorocyclohexane isomers （HCHs） ranged from 0.38 to 39.52 ng.g~, with a mean value of 9.51 ng.g-~. The concentrations of dichlorodiphenyltrichloroethanes （DDTs） and their metabolites were within the ranges of 0.94-700.99 ng. gl, with a mean value of 71.17 ng. g-1. The concentrations of HCHs and DDTs in the soil were lower than the first grade level （50 ng. g-a） of the Chinese Environmental Quality Standard （GB 15618-1995）. Hier- archical Cluster Analysis （HCA） and Pearson＇s bivariate Correlations Analysis （PCA） were used to analyse the distribution and contamination levels of OCPs in this region. The results sh.wed that DDTs were the major contaminants and there were no significant correlations between various OCPs concentrations and the total organic carbon （TOC） contents. A significant positive correlation was observed between HCHs and DDTs （p 〈 0.01）, which indicates that HCHs and DDTs may have similar sources and fate in the study area.

关键词： Jiulong River hexachlorocyclohexane (HCH)and dichlorodiphenyltrichloroethan (DDT) classification,Hierarchical Cluster Analysis (HCA) Pearson's bivariateCorrelations Analysis (PCA)

An improved formula for standard hypoxia tolerance time to evaluate hypoxic tolerance in mice

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Military Medical Research 2019年第2期6卷 111-116页

作者： Gang Xu Yu-Qi Gao Yi-xing Gao Gang Wu Jian-Yang zh.ng Wen-Xiang Gao Institute of Medicine and Hygienic Equipment for High Altitude Region College of High Altitude Military MedicineArmy Medical University(Third Military Medical University)Chongqing400038China Key Laboratory of High Altitude Medicine People’s Liberation ArmyChongqing400038China

Background: Hypoxia is a primary cause of mountain sickness and a common pathological condition in patients with heart failure, sh.ck, stroke, and chronic obstructive pulmonary disease(COPD). Thus far, little advancement in countering hypoxic damage has been achieved, and one of the main reasons is the absence of an ideal algorithm or calculation method to normalize hypoxia tolerance scores when evaluating an animal model. In this study, we improved a traditional calculation formula for assessment of hypoxia ***: We used a sealed bottle model in which the oxygen is gradually consumed by a mouse inside. To evaluate the hypoxia tolerance of mice, the survival time(ST) of the mouse is recorded and was used to calculate standard hypoxia tolerance time(STT) and adjusted standard hypoxia tolerance time(ASTT). Mice administered with methazolamide and saline were used as positive and negative controls, ***: Since mice were grouped according to either body weight(BW) or bottle volume, we found a strongly negative correlation between STT and BW instead of between STT and bottle volume, suggesting that different BWs could cause false positive or negative errors in the STT results. Furthermore, both false positive and negative errors could be rectified when ASTT was used as the evaluation index. Screening for anti-hypoxic medicines by using mice as the experimental subjects would provide more credible results with the improved ASTT method than with the STT ***: ASTT could be a better index than STT for the evaluation of hypoxia tolerance abilities as it could eliminate the impact of animal BW.

关键词： Hypoxia Standard hypoxic tolerance time Mice model Body weight