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The RNAome landscape of tomato during arbuscular mycorrhizal symbiosis reveals an evolving RNA layer symbiotic regulatory network

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Plant Communications 2023年第1期4卷 148-166页

作者： Zhen Zeng Yang Liu xing-Yu Feng Sai-Xi Li xing-mei jiang Jian-Qun Chen Zhu-Qing Shao State Key Laboratory of Pharmaceutical Biotechnology School of Life SciencesNanjing UniversityNanjing 210023China

Arbuscular mycorrhizal symbiosis(AMS)is an ancient plant-fungus relationship that is widely distributed in terrestrial *** formation of symbiotic structures and bidirectional nutrient exchange requires the regulation of numerous ***,the landscape of RNAome during plant AMS involving different types of regulatory RNA is poorly *** this study,a combinatorial strategy utilizing multiple sequencing approaches was used to decipher the landscape of RNAome in tomato,an emerging AMS *** annotation of the tomato genome was improved by a multiple-platform sequencing strategy.A total of 3,174 protein-coding genes were upregulated during AMS,42%of which were alternatively ***-transcriptome analysis revealed that genes from 24 orthogroups were consistently induced by AMS in eight phylogenetically distant *** additional orthogroups were specifically induced by AMS in all surveyed dicot AMS host ***,these orthogroups were absent or not induced in monocots and/or non-AMS hosts,suggesting a continuously evolving AMS-responsive network in addition to a conserved core regulatory ***,we detected 587 lncRNAs,ten miRNAs,and 146 circRNAs that responded to AMS,which were incorporated to establish a tomato AMSresponsive,competing RNA-responsive endogenous RNA(ceRNA)***,a tomato symbiotic transcriptome database(TSTD,https://***/TSTD)was constructed to serve as a resource for deep deciphering of the AMS regulatory *** results help elucidate the reconfiguration of the tomato RNAome during AMS and suggest a sophisticated and evolving RNA layer responsive network during AMS processes.

关键词： tomato RNAome landscape arbuscular mycorrhizal symbiosis evolution ceRNA network

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An angiosperm NLR Atlas reveals that NLR gene reduction is associated with ecological specialization and signal transduction component deletion

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Molecular Plant 2021年第12期14卷 2015-2031页

作者： Yang Liu Zhen Zeng Yan-mei Zhang Qian Li xing-mei jiang Zhen jiang Ji-Hong Tang Dijun Chen Qiang Wang Jian-Qun Chen Zhu-Qing Shao State Key Laboratory of Pharmaceutical Biotechnology School of Life SciencesNanjing UniversityNanjing 210023China jiangsu Key Laboratory for the Research and Utilization of Plant Resources Institute of BotanyJiangsu Province and Chinese Academy of SciencesNanjing 210014China

Nucleotide-binding leucine-rich-repeat(NLR)genes comprise the largest family of plant disease-resis-tance *** NLR genes are phylogenetically divided into the TNL,CNL,and RNL *** copy numbers and subclass composition vary tremendously across angiosperm ***,the evolutionary associations between genomic NLR content and ecological adaptation,or between NLR content and signal transduction components,are poorly characterized because of limited genome *** this study,we established an angiosperm NLR atlas(ANNA,https://***/ANNA/)that includes NLR genes from over 300 angiosperm *** ANNA,we revealed that NLR copy numbers differ up to 66-fold among closely related species owing to rapid gene loss and ***,NLR contraction was associated with adaptations to aquatic,parasitic,and carnivorous *** convergent NLR reduction in aquatic plants resembles the lack of NLR expansion during the long-term evolution of green algae before the colonization of land.A co-evolutionary pattern between NLR subclasses and plant immune pathway components was also identified,suggesting that immune pathway deficiencies may drive TNL ***,we identified a conserved TNL lineage that may function independently of the EDS1-SAG101-NRG1 ***,these findings provide new insights into the evolution of NLR genes in the context of ecological adaptation and genome content variation.

关键词： NLR genes plant disease resistance EDS1 gene family gene family evolution angiosperms

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Realization of Communication Protocols of RFID R/W Device Based on CPLD

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The Journal of China Universities of Posts and Telecommunications 2002年第2期9卷 57-61页

作者： YU xing qi, jiang mei long, DENG Wei chun, SHEN Shu qun (Beijing University of Posts and Telecommunications, Beijing 100876, P.R.China) Beijing University of Posts and Telecommunications Beijing 100876 P.R.China

The function of the communication module of Radio FrequencyIdentification(RFID)system is de- scribed. Based on Electronic DesignAutomation(EDA), the implementation of digital module of RFID usingXIL- INX XC9500 series Complex Programmable Logic Device(CPLD)isintroduced modularized and layering methods In communicationprotocols of RFID systems are employed to enhance the systemperformance and facilitate the de- Sign. To ensure the security andreliability of data transmission, a new method of combing encryptionand error cor- Rection is employed and described in detail.

关键词： RFID EDA CPLD Very High Speed Integrated Circuit Hardware DescriptionLanguage(VHDL)

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Microstructural evolution during the progressive transformation-induced plasticity effect in a Fe-0.1C-5Mnmedium manganese steel

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International Journal of Minerals,Metallurgy and Materials 2025年第2期32卷 369-379页

作者： mei Zhang Wenhao Li Yangfei Chen Yang jiang Xiaofei Guo Han Dong School of Materials Science and Engineering Shanghai UniversityShanghai 200444China State Key Laboratory of Advanced Special Steel Shanghai UniversityShanghai 200444China

The microstructural evolution of a cold-rolled and intercritical annealed medium-Mn steel(Fe-0.10C-5Mn)was investigated during uniaxial tensile ***-situ observations under scanning electron microscopy,transmission electron microscopy,and X-ray diffraction analysis were conducted to characterize the progressive transformation-induced plasticity process and associated fracture initiation *** findings were discussed with the local strain measurements via digital image *** results indicated that Lüders band formation in the steel was limited to 1.5%strain,which was mainly due to the early-stage martensitic phase transformation of a very small amount of the less stable large-sized retained austenite(RA),which led to localized stress concentrations and strain hardening and further retardation of *** small-sized RA exhibited high stability and progressively transformed into martensite and contributed to a stably extended Portevin-Le Chatelier *** volume fraction of RA gradually decreased from 26.8%to 8.2%prior to *** the late deformation stage,fracture initiation primarily occurred at the austenite/martensite and ferrite/martensite interfaces and the ferrite phase.

关键词： medium-Mn steel retained austenite progressive transformation-induced plasticity effect local strain fracture initiation

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Multi-model ensemble learning for battery state-of-health estimation:Recent advances and perspectives

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Journal of Energy Chemistry 2025年第1期100卷 739-759页

作者： Chuanping Lin Jun Xu Delong jiang Jiayang Hou Ying Liang Zhongyue Zou Xuesong mei State Key Laboratory for Manufacturing Systems Engineering Xi'an Jiaotong University Shaanxi Key Laboratory of Intelligent Robots School of Mechanical Engineering Xi'an Jiaotong University Department of Electrical Engineering and Automation Luoyang Institute of Science and Technology School of Mechanical Engineering Hangzhou Dianzi University

The burgeoning market for lithium-ion batteries has stimulated a growing need for more reliable battery performance monitoring. Accurate state-of-health(SOH) estimation is critical for ensuring battery operational performance. Despite numerous data-driven methods reported in existing research for battery SOH estimation, these methods often exhibit inconsistent performance across different application scenarios. To address this issue and overcome the performance limitations of individual data-driven models,integrating multiple models for SOH estimation has received considerable attention. Ensemble learning(EL) typically leverages the strengths of multiple base models to achieve more robust and accurate outputs. However, the lack of a clear review of current research hinders the further development of ensemble methods in SOH estimation. Therefore, this paper comprehensively reviews multi-model ensemble learning methods for battery SOH estimation. First, existing ensemble methods are systematically categorized into 6 classes based on their combination strategies. Different realizations and underlying connections are meticulously analyzed for each category of EL methods, highlighting distinctions, innovations, and typical applications. Subsequently, these ensemble methods are comprehensively compared in terms of base models, combination strategies, and publication trends. Evaluations across 6 dimensions underscore the outstanding performance of stacking-based ensemble methods. Following this, these ensemble methods are further inspected from the perspectives of weighted ensemble and diversity, aiming to inspire potential approaches for enhancing ensemble performance. Moreover, addressing challenges such as base model selection, measuring model robustness and uncertainty, and interpretability of ensemble models in practical applications is emphasized. Finally, future research prospects are outlined, specifically noting that deep learning ensemble is poised to advance ens

关键词： Lithium-ion battery State-of-health estimation Data-driven Machine learning Ensemble learning Ensemble diversity

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Reduced mesencephalic astrocyte-derived neurotrophic factor expression by mutant androgen receptor contributes to neurodegeneration in a model of spinal and bulbar muscular atrophy pathology

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Neural Regeneration Research 2025年第9期20卷 2655-2666页

作者： Yiyang Qin Wenzhen Zhu Tingting Guo Yiran Zhang Tingting xing Peng Yin Shihua Li Xiao-jiang Li Su Yang Guangdong Key Laboratory of Non-human Primate Research Key Laboratory of CNS Regeneration(Ministry of Education)Guangdong–HongKong–Macao Institute of CNS RegenerationJinan UniversityGuangzhouGuangdong ProvinceChina

Spinal and bulbar muscular atrophy is a neurodegenerative disease caused by extended CAG trinucleotide repeats in the androgen receptor gene,which encodes a ligand-dependent transcription facto *** mutant androgen receptor protein,characterized by polyglutamine expansion,is prone to misfolding and forms aggregates in both the nucleus and cytoplasm in the brain in spinal and bulbar muscular atrophy *** aggregates alter protein-protein interactions and compromise transcriptional *** this study,we reported that in both cultured N2a cells and mouse brain,mutant androgen receptor with polyglutamine expansion causes reduced expression of mesencephalic astrocyte-de rived neurotrophic *** n of mesencephalic astrocyte-derived neurotrophic factor amelio rated the neurotoxicity of mutant androgen receptor through the inhibition of mutant androgen receptor *** down endogenous mesencephalic astrocyte-derived neurotrophic factor in the mouse brain exacerbated neuronal damage and mutant androgen receptor *** findings suggest that inhibition of mesencephalic astrocyte-derived neurotrophic factor expression by mutant androgen receptor is a potential mechanism underlying neurodegeneration in spinal and bulbar muscular atrophy.

关键词： androgen receptor mesencephalic astrocyte-derived neurotrophic factor mouse model neurodegeneration neuronal loss neurotrophic factor polyglutamine disease protein misfolding spinal and bulbar muscular atrophy transcription factor

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Loss-of-function mutations of microRNA-142-3p promote ASH1L expression to induce immune evasion and hepatocellular carcinoma progression

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World Journal of Gastroenterology 2025年第1期31卷 126-145页

作者： xing-Hui Yu Yan Xie Jian Yu Kun-Ning Zhang Zhou-Bo Guo Di Wang Zhao-Xian Li Wei-Qi Zhang Yu-Ying Tan Li Zhang Wen-Tao jiang School of Medicine Nankai UniversityTianjin 300192China Tianjin Key Laboratory of Molecular Diagnosis and Treatment of Liver Cancer Tianjin First Center HospitalTianjin 300192China Department of Liver Transplantation Tianjin First Center HospitalTianjin 300192China First Central Clinical School Tianjin Medical UniversityTianjin 300192China

BACKGROUND Hepatocellular carcinoma(HCC)has been a pervasive malignancy throughout the world with elevated *** therapeutic targets are beneficial to treat and predict the ***,the exact molecular mechanisms leading to the progression of HCC are still *** has shown that the microRNA-142-3p level decreases in HCC,whereas bioinformatics analysis of the cancer genome atlas database shows the ASH1L expression increased among liver tumor *** this paper,we will explore the effects and mechanisms of microRNA-142-3p and ASH1L affect the prognosis of HCC patients and HCC cell bioactivity,and the association between *** To investigate the effects and mechanisms of microRNA-142-3p and ASH1L on the HCC cell bioactivity and prognosis of HCC *** In this study,we grouped HCC patients according to their immunohistochemistry results of ASH1L with pathological tissues,and retrospectively analyzed the prognosis of HCC ***,explored the roles and mechanisms of microRNA-142-3p and ASH1L by cellular and animal experiments,which involved the following experimental methods:Immunohistochemical staining,western blot,quantitative real-time-polymerase chain reaction,flow cytometric analysis,tumor xenografts in nude mice,*** statistical methods involved in this study contained t-test,one-way analysis of variance,theχ^(2)test,the Kaplan-meier approach and the log-rank *** In this study,we found that HCC patients with high expression of ASH1L possess a more recurrence rate as well as a decreased overall survival ***1L promotes the tumorigenicity of HCC and microRNA-142-3p exhibits reduced expression in HCC tissues and interacts with ASH1L through targeting the ASH1L 3′untranslated ***,microRNA-142-3p promotes apoptosis and inhibits proliferation,invasion,and migration of HCC cell lines in vitro via *** the exploration mechanism,we found ASH1L may promote an immunosuppressive mi

关键词： Hepatocellular carcinoma MicroRNA-142-3p ASH1L Immune evasion Tumor immune microenvironment Apoptosis

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重庆市农村畜禽养殖废水的污染及对策

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三峡环境与生态 2011年第5期33卷 60-62页

作者：渠巍熊强幸梅姜瑜重庆市环境监测中心重庆410047

畜禽养殖产生的废水已经成为农村面源污染不可忽视的污染源。通过对典型的重庆市农村集约化畜禽养殖场和经营性非规模化养殖场养殖废水中COD,NH3-N和TP浓度的分析和研究,提出了对重庆市农村畜禽养殖业发展的意见。

关键词：农村畜禽养殖废水污染对策

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Analysis of the anti-T2DM components in dichloromethane fraction of Schisandra sphenanthera and its mechanism

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Traditional Medicine Research 2025年第1期10卷 27-43页

作者： Shi-Bo Feng Hai-Hui jiang Hui-Li Kang Yi jiang Hua-Wei Zhang Xiao-mei Song Yu-Ze Li Wen-Li Huang Dong-Dong Zhang Chong Deng School of Pharmacy Shaanxi University of Chinese MedicineXianyang 712046China School of Pharmacy and Shaanxi Qinling Application Development and Engineering Center of Chinese Herbal Medicine Shaanxi University of Chinese MedicineXianyang 712046China Key Laboratory for Research and Development of“Qin Medicine”of Shaanxi Administration of Traditional Chinese Medicine Xianyang 712046China Shaanxi Key Laboratory of Research and Application of“Taibai Qi Yao” Xianyang 712046China

Background:The type 2 diabetes mellitus(T2DM)pharmacodynamic study of various parts of Schisandra sphenanthera was conducted in the previous stage,and it was found that dichloromethane extracted part(SDP)had a significant hypoglycemic ***,the components of SDP were analyzed,and the specific mechanism of its anti-T2DM was ***:We used a high-fat,high-sugar diet in combination with streptozotocin to induce a T2DM rat model,and the model rats were divided into two groups according to body weight and blood ***,oral glucose tolerance test,fasting blood glucose,low density lipoprotein cholesterol,superoxide dismutase,insulin,glycated hemoglobin,total cholesterol,nonesterified free fatty acids,alanine aminotransferase,high-density lipoprotein cholesterol,aspartate aminotransferase,malondialdehyde,and glutathione peroxidase were measured,organ indices were calculated,and pathological sections of pancreas and liver were *** 16S rRNA V3–V4 region of intestinal flora was sequenced to explore the effect of SDP on biochemical indicators and intestinal *** on the above indicators,the anti-T2DM mechanism of SDP in Schisandra sphenanthera was ***:After six weeks of administration,the biochemical indices of diabetic rats were diminished compared to the control *** SDP could significantly increase the gut microbialα-diversity index,resulting in significant changes in the flora of T2DM rats,with increased richness and diversity,reduced harmful flora,and significantly back-regulated the levels of acetic acid,propionic acid,and butyric ***:SDP can improve the symptoms associated with elevated blood glucose,dyslipidemia,elevated fasting insulin levels,and damaged glucose tolerance in *** against T2DM may be through the control of intestinal flora to normalize and exert anti-diabetic effect;its main active components may be lignans and terpenoids.

关键词： Schisandra sphenanthera T2DM intestinal flora HPLC

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CX3 chemokine receptor 1 deficiency leads to reduced dendritic complexity and delayed maturation of newborn neurons in the adult mouse hippocampus

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Neural Regeneration Research 2015年第5期10卷 772-777页

作者： Feng Xiao Jun-mei Xu xing-hua jiang Department of Anesthesiology the Second Xiangya Hospital of Central South University

Previous studies have shown that microglia impact the proliferation and differentiation of neu- rons during hippocampal neurogenesis via the fractalkine/CX3 chemokine receptor i （CX3CRI） signaling pathway. However, whether microglia can influence the maturation and dendritic growth of newborn neurons during hippocampal neurogenesis remains unclear. In the present study, we found that the number of doublecortin-positive cells in the hippocampus was decreased, and the dendritic length and number of intersections in newborn neurons in the hippocampus were reduced in transgenic adult mice with CX3CR1 deficiency （CX3CRl^GFP/GFe）. Furthermore, after experimental seizures were induced with kainic acid in these CX3CRl-deficient mice, the expression of c-fos, a marker of neuronal activity, was reduced compared with wild-type mice. Collectively, the experimental findings indicate that the functional maturation of newborn neu- rons during hippocampal neurogenesis in adult mice is delayed by CX3CR1 deficiency.

关键词： nerve regeneration fractalkine CX3 chemokine receptor 1 neuronal maturation dendrites doublecortin synaptic maturation newborn neurons neural regeneration

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