检索结果-南通市图书馆

Effects of IκBα and its mutants on NF-κB and p53 signaling pathways

World Journal of Gastroenterology 2006年第41期12卷 6658-6664页

作者： Xian Li Da xing Ju wang De-Bin Zhu Lan Zhang Xiao-Jia Chen fen-Yong Sun An Hong Institute of Laser Life Science South China Normal University Guangzhou 510631 Guangdong Province China Institute of Biology Engineering Jinan University Guangzhou 510632 Guangdong Province China

AIM： To study the effects of IκBα and its mutants （IκBαM, IκBα3N, IκBαM44C） on NF-κB, p53 and their downstream target genes. The relationship of NF-κB, p53, and IκBα was further discussed. METHODS： pECFP-IκBα, pECFP-IκBαM （amino acides 1-317, Ser32, 36A）, pECFP-IκBα243N （amino acides 1-243）, pECFP-IκBα244C （amino acides 24±317）, pEYFP-p65 and pp53-DsRed were constructed and transfected to ASTC-α-1 cells. Cells were transfected with pECFP-Cl as a control. 30 h after the transfection, location patterns of NF-κB, p53 and IκBα（IκBαM, IκBα243N, IκBα224C） were observed by a laser scanning microscope （LSM510/ConfoCor2, Zeiss）. RNA extraction and reverse transcription were performed in cells transfected or co-transfected with different plasmids. Effects of IκBα and its mutants on the transprition level of NF-κB, NF-κB downstream target gene TNF-α, p53 and p53 downstream target gene Bax were observed by real time QT-PCR. In all experiments β-actin was reference. Results are expressed as the target/reference ratio of the sample divided by the target/reference ratio of the control. Different transfected cells were incubated with CCK-8 for 2 h in the incubator. Then the absorbance at 450 nm was measured by using a microplate reader. RESULTS： Cells that were transfected with p53- DsRed revealed a predominant nuclear localization. YFP-p65 mainly existed in the cytoplasm. Cells were transfected with CFP-IκBα, CFP-IκBαM, and CFP-IκBα243N respectively and revealed a predominant cytosolic localization. However, cells transfected of CFP-IκBα244C revealed a predominant nuclear localization. The rnRNA levels of p65, TNF-α, p53 and Bax in CFP-IκBα transfected cells did not change significantly, while in YFP-p65/CFP-IκBα co-transfected cells, IκBα decreased the transcription of p65 downstream gene TNF-α （2.24 ± 0.503） compared with the YFP-p65/ CFP-C1 co-transfected cells （5.08 ± 0.891）（P 〈 0.05）. Phosphorylation defective IκBα （IκBαM） decreased the transcription levels of all the four g

关键词： Nuclear factor-κB Inhibitor of NF-κB alpha p53 Real-time QT-PCR

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Current status of genetic transformation technology developed in cucumber(Cucumis sativus L.)

引用

Journal of Integrative Agriculture 2015年第3期14卷 469-482页

作者： wang Shun-li Seong Sub Ku YE xing-guo HE Cong-fen Suk Yoon Kwon Pil Son Choi Key Laboratory of Biology and Genetic Improvement of Horticultural Crops Ministry of Agriculture/Institute of Vegetables and Flowers Chinese Academy of Agricultural Sciences Department of Oriental Pharmaceutical Development Nambu University Institute of Crop Science Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement School of Sciences Beijing Technology and Business University Plant Systems Engineering Research Center

Genetic transformation is an important technique for functional genomics study and genetic improvement of plants. Until now, Agrobacterium-mediated transformation methods using cotyledon as explants has been the major approach for cucumber, and its frequency has been up to 23%. For example, significantly enhancement of the transformation efficiency of this plant species was achieved from the cotyledon explants of the cultivar Poinsett 76 infected by Agrobacterium strains EHA105 with efficient positive selection system in lots of experiments. This review is to summarize some key factors influencing cucumber regeneration and genetic transformation, including target genes, selection systems and the ways of transgene introduction, and then to put forward some strategies for the increasing of cucumber transformation efficiency. In the future, it is high possible for cucumber to be potential bioreactor to produce vaccine and biomaterials for human beings.

关键词： Cucumis sativus L. plant regeneration genetic transformation positive selection system

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Novel Synthesis of 2-Amino-3-ethoxycarbonyl Benzopyrans with Onium Salt as Soluble Support

引用

Chemical Research in Chinese Universities 2011年第3期27卷 435-440页

作者： ZHANG Li LU Cui-fen YANG Gui-chun CHEN Zu-xing Ministry-of-Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules. Faculty of Chemistry and Chemical Engineering Hubei University Wuhan 430062 P. R. China

Task specific onium salt has been presented as soluble support in the synthesis of benzopyrans in this paper. This method allows for results by far superior to those reported other soluble supports and allows for very... 详细信息

关键词： Onium salt Support Benzopyran

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effects of selenium source and level on growth performance, antioxidative ability and meat quality of broilers

引用

Journal of Integrative Agriculture 2021年第1期20卷 227-235页

作者： wang Chuan-long xing Guan-zhong wang Li-sai LI Su-fen ZHANG Li-yang LU Lin LUO Xu-gang LIAO Xiu-dong Mineral Nutrition Research Division Institute of Animal SciencesChinese Academy of Agricultural SciencesBeijing 100193P.R.China Poultry Mineral Nutrition Laboratory College of Animal Science and TechnologyYangzhou UniversityYangzhou 225000P.R.China Department of Animal Science Hebei Normal University of Science and TechnologyQinhuangdao 066004P.R.China

This experiment was conducted to investigate the effects of selenium(Se) source and level on growth performance, carcass traits, antioxidative ability and meat quality of broilers. A total of 320 one-d-old Arbor Acres commercial broilers were randomly assigned to 1 of 5 treatments with 8 replicates in a completely randomized design involving a 2×2 factorial arrangement of treatments plus one Se-unsupplemented basal diet control for 42 d. The two Se sources were sodium selenite and Se yeast, and the two supplemental Se levels were 0.20 and 0.40 mg Se kg^–1. The results showed that broilers fed the Se-supplemented diets had higher(P<0.05) average daily gain and average daily feed intake from 22 to 42 d of age, eviscerated yield and abdominal fat percentages, Se concentrations and glutathione peroxidase(GSH-Px) activities in breast and thigh muscles on d 42, and lower(P<0.05) feed/gain from 1 to 21 and 22 to 42 d of age, mortality from 22 to 42 d of age and malondialdehyde(MDA) concentration in thigh muscle on d 42 than those fed the control diet. Broilers fed the diets supplemented with Se yeast had higher(P<0.05) p H value and lower(P<0.05) shear force in thigh muscle than those fed the diets supplemented with sodium selenite. Additionally, broilers fed the diets supplemented with 0.40 mg Se kg^–1 had lower(P<0.05) shear force in thigh muscle and higher(P<0.05) GSH-Px activities in breast and thigh muscles than those fed the diets supplemented with 0.20 mg Se kg^–1. Furthermore, broilers fed the diet supplemented with Se yeast at 0.40 mg Se kg^–1 had higher(P<0.05) Se concentrations in breast and thigh muscles than those fed the diet supplemented with Se yeast at 0.20 mg Se kg^–1, but no differences(P<0.05) were observed in these indices of broilers fed the diets supplemented with sodium selenite between 0.20 and 0.40 mg Se kg^–1. The results from the present study indicated that supplemental Se could increase the growth performance, muscle Se concentration and antio

关键词： selenium source growth performance meat quality antioxidative ability broiler

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Inhibition of the immunoproteasome LMP_(2) ameliorates ischemia/hypoxia-induced blood–brain barrier injury through the Wnt/β-catenin signalling pathway

引用

Military Medical Research 2022年第4期9卷 404-418页

作者： xing-Yong Chen Shao-fen Wan Nan-Nan Yao Ze-Jing Lin Yan-Guang Mao Xiao-Hua Yu Yin-Zhou wang Department of Neurology Fujian Provincial HospitalShengli Clinical Medical College of Fujian Medical UniversityNo.134DongjieGulou DistrictFuzhou 350001China Fujian Academy of Medical Science Fuzhou 350o01China Key Testing Laboratory of Fujian Province Fuzhou 350001China.

Background:Disruption of the blood–brain barrier(BBB)after a stroke can lead to brain injury and neurological *** work confirmed the involvement of the immunoproteasome subunit of low molecular mass peptide 2(LMP2)in the pathophysiology of ischemia ***,the relationship between the immunoproteasome LMP2 and the BBB remains ***:Adult male Sprague–Dawley rats were subjected to transient middle cerebral artery occlusion/reperfusion(MCAO/R).Three days before MCAO,the rats were treated with lentivirus-mediated LMP2 shRNA preparations by stereotactical injection into the ipsilateral hemispheric *** rat brain microvascular endothelial cell(RBMVEC)line was exposed to oxygen–glucose deprivation/reperfusion(OGD/R)to mimic ischemic conditions in *** RNA interference-mediated knockdown of LMP2 orβ-catenin was analysed in vivo and in *** of the quantity of extravasated Evans blue(EB)and cerebral fluorescent angiography were performed to evaluate the integrity of the *** and Western blotting were employed to detect the expression of target *** migration was evaluated using a scratch migration *** results of immunofluorescence,Western blotting and cell migration were quantified using the software ImageJ(Version 1.53).Parametric data from different groups were compared using one-way ANOVA followed by the least significant difference(LSD)***:Cerebral ischemia led to lower levels of structural components of the BBB such as tight junction proteins[occludin,claudin-1 and zonula occludens(ZO-1)]in the MCAO/R group compared with the sham group(P<0.001).However,inhibition of the immunoproteasome LMP2 restored the expression of these proteins,resulting in higher levels of occludin,claudin-1 and ZO-1 in the LMP2-shRNA group compared with the control-shRNA group(P<0.001).In addition,inhibition of the immunoproteasome LMP2 contributed to higher microvascular density and decreased BBB perm

关键词： Immunoproteasome Blood–brain barrier Wnt/β-catenin pathway Oxygen–glucose deprivation/reperfusion Cerebral ischemia

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Low-amplitude structure recognition method based on non-subsampled contourlet transform

引用

Petroleum Science 2024年第5期21卷 3062-3078页

作者： fen Lyu xing-Ye Liu Li Chen Chao Li Jie Zhou Huai-Lai Zhou Key Lab of Earth Exploration&Information Techniques of Ministry of Education Chengdu University of TechnologyChengdu610059SichuanChina College of Geophysics Chengdu University of TechnologyChengdu610059SichuanChina State Key Laboratory of Oil and Gas Reservoir Geology and Exploitation Chengdu University of TechnologyChengdu610059SichuanChina Postdoctoral Research Station of the School of Geophysics Chengdu University of TechnologyChengdu610059SichuanChina

Currently, horizontal well fracturing is indispensable for shale gas development. Due to the variable reservoir formation morphology, the drilling trajectory often deviates from the high-quality reservoir,which increases the risk of fracturing. Accurately recognizing low-amplitude structures plays a crucial role in guiding horizontal wells. However, existing methods have low recognition accuracy, and are difficult to meet actual production demand. In order to improve the drilling encounter rate of high-quality reservoirs, we propose a method for fine recognition of low-amplitude structures based on the non-subsampled contourlet transform(NSCT). Firstly, the seismic structural data are analyzed at multiple scales and directions using the NSCT and decomposed into low-frequency and high-frequency structural components. Then, the signal of each component is reconstructed to eliminate the low-frequency background of the structure, highlight the structure and texture information, and recognize the low-amplitude structure from it. Finally, we combined the drilled horizontal wells to verify the low-amplitude structural recognition results. Taking a study area in the west Sichuan Basin block as an example, we demonstrate the fine identification of low-amplitude structures based on NSCT. By combining the variation characteristics of logging curves, such as organic carbon content(TOC), natural gamma value(GR), etc., the real structure type is verified and determined, and the false structures in the recognition results are checked. The proposed method can provide reliable information on low-amplitude structures for optimizing the trajectory of horizontal wells. Compared with identification methods based on traditional wavelet transform and curvelet transform, NSCT enhances the local features of low-amplitude structures and achieves finer mapping of low-amplitude structures, showing promise for application.

关键词： Shale gas Low-amplitude structure Low-frequency background Non-subsampled contourlet transform Horizontal well verification

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

A novel approach for synthesis of 3-amino-5-aryl-2,5-dihydropyridazines using onium salt as soluble support

引用

Chinese Chemical Letters 2011年第7期22卷 823-826页

作者： Meng Gao Cui fen Lu Gui Chun Yang Zu xing Chen Ministry-of-Education Key Laboratory for the Synthesis and Application of Organic Functional Molecule & School of Chemistry and Chemical Engineering Hubei University Wuhan 430062 China

In this work it was presented an application of task specific onium salt as soluble support for the synthesis of 3-amino-5-aryl-2,5- dihydropyridazines. This soluble support is of wide applicability and combines advantages of solid phase synthesis without its limitations with those of solution phase chemistry. After a simple washing step, products were cleaved from the supports and obtained in good yields.

关键词： Onium salt Support Synthesis Pyridazines

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Organic field-effect transistor floating-gate memory using polysilicon as charge trapping layer

引用

Chinese Physics B 2019年第8期28卷 282-286页

作者： Wen-Ting Zhang fen-Xia wang Yu-Miao Li Xiao-xing Guo Jian-Hong Yang Institute of Microelectronics Lanzhou UniversityLanzhou 730000China School of Electronic and Information Engineering Lanzhou Jiaotong UniversityLanzhou 730070China

In this study,we present an organic field-effect transistor floating-gate memory using polysilicon(poly-Si)as a charge trapping *** memory device is fabricated on a N^+-Si/SiO2 ***-Si,polymethylmethacrylate,and pentacene are used as a floating-gate layer,tunneling layer,and active layer,*** device shows bidirectional storage characteristics under the action of programming/erasing(P/E)operation due to the supplied electrons and holes in the channel and the bidirectional charge trapping characteristic of the poly-Si *** carrier mobility and switching current ratio(Ion/Ioff ratio)of the device with a tunneling layer thickness of 85 nm are 0.01 cm^2·V^-1·s^-1 and 102,respectively.A large memory window of 9.28 V can be obtained under a P/E voltage of±60 V.

关键词： organic floating-gate memory polysilicon floating-gate memory window

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

玉米大豆间作对喀斯特区土壤理化性质及微生物碳代谢特征的影响

引用

作物学报 2025年第1期51卷 273-284页

作者：钱玉平宿兵兵高吉星阮粉花李亚伟茅林春滇西应用技术大学普洱茶学院云南普洱665000 兰州交通大学环境与市政工程学院甘肃兰州730070 甘肃省农业科学院作物研究所甘肃兰州730070 浙江大学生物系统工程与食品科学学院浙江杭州310000

为探明喀斯特区玉米大豆带状复合种植对土壤理化性质及微生物群落结构多样性的影响,本研究设置玉米大豆间作(MSI)、玉米单作(MM)和大豆单作(SM) 3种模式,采用Biolog-ECO微孔培养法,旨在揭示玉米大豆种植模式对土壤微生物碳源代谢活性、... 详细信息

为探明喀斯特区玉米大豆带状复合种植对土壤理化性质及微生物群落结构多样性的影响,本研究设置玉米大豆间作(MSI)、玉米单作(MM)和大豆单作(SM) 3种模式,采用Biolog-ECO微孔培养法,旨在揭示玉米大豆种植模式对土壤微生物碳源代谢活性、多样性以及土壤性质的影响及其机制。结果表明,与MM及SM相比, MSI土壤微生物群落丰富度指数(McIntoshindex)分别显著提高了11.90%和58.40%,平均颜色变化率(averagewellcolordevelopment, AWCD)分别显著增加了24.50%和80.10%,羧酸类、氨基酸类和酚酸类碳源的平均相对吸光度分别显著提高了34.50%、63.70%和61.80%;碳源代谢指纹图谱表明,MSI模式中土壤微生物通过提高衣康酸的代谢活性进而增加了对羧酸类碳源的利用,通过提高L-苯丙氨酸、L-苏氨酸和甘氨酰-L-谷氨酸的代谢活性从而增加了氨基酸类碳源利用,通过提高吐温40、吐温80和肝糖的代谢活性从而增加了对多聚类碳源的利用;同时, MSI处理土壤SOC分别较MM和SM显著提高8.50%和72.84%, NH_4~+-N和TN含量分别较SM处理显著增加46.70%和33.30%;主成分分析表明,提取的2个主成分解释了碳源利用总变异的79.69%,种植模式对碳源代谢的综合利用能力表现为MSI>MM>SM,其中MSI土壤微生物群落对羧酸类、氨基酸类和多聚类代谢利用能力最强;冗余分析则表明,显著影响碳源代谢利用的2个环境因子分别是TN (53.50%)和SOC (30.90%),其中TN促进了羧酸类和氨基酸类碳源的代谢利用, SOC加强了胺类和酚酸类碳源的利用。综上可见,玉米大豆间作模式土壤微生物碳代谢的偏好性主要由微生物群落结构多样性引起,同时又受土壤全氮和有机质含量的调控,表明微生物群落结构与土壤理化因子间的互作可能是大豆玉米复合种植增产增效的一个关键因素。

关键词：玉米大豆土壤微生物碳源代谢活性土壤理化性质 Biolog-ECO

来源：

维普期刊数据库

同方期刊数据库博看期刊

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Comprehensive evaluation of high-steep slope stability and optimal high-steep slope design by 3D physical modeling

引用

International Journal of Minerals,Metallurgy and Materials 2015年第1期22卷 1-11页

作者： xing-ping Lai Peng-fei Shan Mei-feng Cai fen-hua Ren Wen-hui Tan School of Energy Engineering Xi'an University of Science and Technology School of Civil and Environmental Engineering University of Science and Technology Beijing Key Laboratory of the Ministry of Education of China for Efficient Mining and Safety of Metal Mines University of Science and Technology Beijing

High-steep slope stability and its optimal excavation design in Shuichang open pit iron mine were analyzed based on a large 3D physical simulation technique. An optimal excavation scheme with a relatively steeper slope angle was successfully implemented at the northwest wall between Nos. 4 and 5 exploration lines of Shuichang Iron Mine, taking into account the 3D scale effect. The phys-ico-mechanical properties of rock materials were obtained by laboratory tests conducted on sample cores from exploration drilling directly from the iron mine. A porous rock-like composite material was formed for the model, and the mechanical parameters of the material were assessed experimentally;specifically, the effect of water on the sample was quantitatively determined. We adopted an experimental setup using stiff modular applied static loading to carry out a visual excavation of the slope at a random depth. The setup was equipped with acous-tic emission （AE） sensors, and the experiments were monitored by crack optical acquirement, ground penetrating radar, and close-field pho-togrammetry to investigate the mechanisms of rock-mass destabilization in the high-steep slope. For the complex study area, the model re-sults indicated a clear correlation between the model＇s destabilization resulting from slope excavation and the collected monitoring informa-tion. During the model simulation, the overall angle of the slope increased by 1-6 degrees in different sections. Dramatically, the modeled excavation scheme saved over 80 million tons of rock from extraction, generating enormous economic and ecological benefits.

关键词： slope stability analysis excavation design three-dimensional models physical simulation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：