检索结果-南通市图书馆

Promoter methylation and mRNA expression of DKK-3 and WIF-1 in hepatocellular carcinoma

World Journal of Gastroenterology 2009年第21期15卷 2595-2601页

作者： Zhen Ding Ye-Ben qian Li-xin zhu qi-Ru Xiong Department of Surgery First Affiliated Hospital of Anhui Medical University 218 Jixi avenue Hefei 230022 Anhui Province. China

AIM:To investigate the promoter methylation status and mRNA expression of DKK-3 and WIF-1 gene in hepatocellular carcinoma(HCC).METHODS:DKK-3 and WIF-1 acted as Wnt-antagonists and tumor suppressors,but hypermethylation of the gene promoter and low mRNA expression activated Wnt signaling aberrantly and induced the development of *** status of the DKK-3 and WIF-1 gene promoter was investigated using methylation specific polymerase chain reaction(PCR) in tumor and adjacent non-cancerous tissues from 33 HCC patients and 20 normal liver tissues served as *** expression of DKK-3 and WIF-1 mRNA was also determined by real-time quantitative reverse transcriptase *** relationship between methylation,mRNA expression,and clinical data,as well as methylation and mRNA expression of the two genes were ***:The methylation of DKK-3 and WIF-1 genes in HCC increased significantly compared with adjacent non-cancerous tissues and normal control tissues(χ2 =7.79,P < 0.05;χ2 = 4.89,P < 0.05),and no significant difference in methylation between adjacent non-cancerous tissues and normal control tissues was *** HCC tissues,significant differences in the DKK-3 promoter methylation were observed in age and cirrhosis,and significant differences of the WIF-1 promoter methylation were observed in HBsAg and *** average expression of DKK-3 mRNA in HCC and adjacent non-cancerous tissues was increased significantly compared with normal control *** average expression of WIF-1 mRNA showed no significant difference among the three *** mRNA expression of DKK-3 gene in HCC was decreased as the pathological grade ***:The aberrant promoter methylation and decreased expression of DKK-3 and WIF-1 may be an important mechanism in HCC,and may be a far-reaching significance in early diagnosis and therapy of HCC.

关键词： Hepatocellular carcinoma DKK-3 WIF-1 Promoter methylation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Stereospecific access to bridged[n.2.1]skeletons through gold-catalyzed tandem reaction of indolyl homopropargyl amides

引用

Chinese Chemical Letters 2020年第5期31卷 1309-1312页

作者： Tong-De Tan xin-qi zhu Mei Jia Yongjia Lin Jun Cheng Yuanzhi Xia Long-Wu Ye iChEM State Key Laboratory of Physical Chemistry of Solid Surfaces and Key Laboratory for Chemical Biology of Fujian ProvinceCollege of Chemistry and Chemical EngineeringXiamen UniversityXiamen 361005China College of Chemistry and Materials Engineering Wenzhou UniversityWenzhou 325035China State Key Laboratory of Organometallic Chemistry Chinese Academy of SciencesShanghai 200032China

An efficient gold-catalyzed anti-Markovnikov cycloisomerization-initiated tandem reaction of Bocprotected indole tethered homopropargyl amides has been *** method delivers a wide range of valuable bridged aza-[n.2.1]skeletons(n=3-7)at room temperature with high diastereoselectivity and enantioselectivity by a chirality-transfer ***,the gold-catalyzed tandem reaction of homopropargyl alcohol is also achieved to produce the bridged oxa-[3.2.1]skeleton.

关键词： Gold Heterocycles Cascade Homogeneous catalysis Alkynes

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Circadian disturbance induces erectile dysfunction by impairing endothelial function

引用

Asian Journal of Andrology 2024年第2期26卷 205-211页

作者： Tao Li Yi-Ting Jiang xin-zhu qi Peng Chen Jun-Hao Zhang Fu Luo Jun qiao Jiang Gu Guang-Shi Du qiang Wang Department of Urology Affiliated Hospital of Guizhou Medical UniversityGuiyang 550004China Department of Urology Guizhou Provincial People's HospitalGuiyang 550002China Department of Otorhinolaryngology Affiliated Hospital of Guizhou Medical UniversityGuiyang 550004China Guizhou Institute for Food and Drug Control Guiyang 550004China Department of Reproductive Center Guizhou Provincial People's HospitalGuiyang 550002China Translational Medicine Research Center of Guizhou Medical University Guiyang 550025China

In order to explore the impact of circadian disturbance on erectile function,we randomly divided 24 adult male rats into groups of control(light on at 8:00 *** off at 8:00 p.m.),dark/dark(DD;constant dark),light/light(LL;constant light),and shift dark/light(DL;light off at 8:00 *** on at 8:0o p.m.).Four weeks later,erectile function was measured and corpora cavernosa were harvested for *** maximum intracavernous pressure(mlcP)and mCP/mean arterial pressure(MAP)ratio in the DD,LL,and DL groups were significantly lower than that in the control *** LL and DL groups showed significantly attenuated endothelial nitric oxide synthase(eNOS),while DD,LL,and DL showed reduced neuronal nitric oxide synthase(nNOS)at both mRNA and protein *** production of nitric oxide(NO)and cyclic guanosinemonophosphate(cGMP)was inhibited by altered light/dark cycles to varying *** disturbance impaired endothelial function and contributed to erectile *** the core circadian elements,mRNA expression of circadian locomotor output cycles kaput(Clock)and brain/muscle aryl-hydrocarbon receptor nuclear translocator-like protein 1(Bmal1)was elevated in the DL group,but their protein expression was not significantly ***,LL,and DL increased period 1(Per1)and Per3 levels,while LL and DL increased PER1 *** significant difference was found for Per2levels,and PER2 and PER3 concentrations were not significantly ***,LL and DL significantly increased cryptochrome-1(CRY1)and CRY2 at both mRNA and protein *** altered light/dark rat model showed that circadian disturbance contributed to erectile dysfunction probably by impairing endothelial ***,the core circadian elements were detected in the corpora cavernosa,but these were ***,which circadian element regulates erectile function and how it works need further analysis.

关键词： circadian disturbance endothelial function erectile function light/dark cycle NO/cGMP

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Br■nsted acid-catalyzed asymmetric dearomatization of indolyl ynamides: Practical and enantioselective synthesis of polycyclic indolines

引用

Chinese Chemical Letters 2023年第3期34卷 317-321页

作者： Zhi-xin Zhang Xuan Wang Jia-Tian Jiang Jie Chen xin-qi zhu Long-Wu Ye Key Laboratory for Chemical Biology of Fujian Province and State Key Laboratory of Physical Chemistry of Solid Surfaces College of Chemistry and Chemical EngineeringXiamen UniversityXiamen 361005China State Key Laboratory of Elemento-Organic Chemistry Nankai UniversityTianjin 300071China State Key Laboratory of Organometallic Chemistry Shanghai Institute of Organic ChemistryChinese Academy of SciencesShanghai 200032China

Catalytic asymmetric dearomatization of indoles and alkynes has received much attention in the past decade because this strategy offers an attractive and alternative way for the efficient synthesis of valuable chiral polycyclic indolines. However, these reactions have been mostly limited to transition-metal catalysts, and the related chiral Br■nsted acid catalysis has been scarcely reported. Herein, we disclose a chiral phosphoric acid-catalyzed asymmetric dearomatization of indolyl ynamides by direct activation of alkynes. This metal-free method enables the practical and atom-economical construction of an array of valuable chiral polycyclic indolines in moderate to good yields with high enantioselectivities.

关键词： Br■nsted acid catalysis Dearomatization Heterocycles Ynamides Stereoselectivity

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Role of the globus pallidus in motor and non-motor symptoms of Parkinson's disease

引用

Neural Regeneration Research 2025年第6期20卷 1628-1643页

作者： Yimiao Jiang Zengxin qi Huixian zhu Kangli Shen Ruiqi Liu Chenxin Fang Weiwei Lou Yifan Jiang Wangrui Yuan xin Cao Liang Chen qianxing zhuang Department of Physiology School of MedicineNantong UniversityNantongJiangsu ProvinceChina Department of Neurosurgery Huashan HospitalShanghai Medical CollegeFudan UniversityShanghaiChina National Center for Neurological Disorders ShanghaiChina Shanghai Key Laboratory of Brain Function Restoration and Neural Regeneration ShanghaiChina State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science Institute of Brain ScienceFudan UniversityShanghaiChina

The globus pallidus plays a pivotal role in the basal ganglia circuit. Parkinson's disease is characterized by degeneration of dopamine-producing cells in the substantia nigra, which leads to dopamine deficiency in the brain that subsequently manifests as various motor and non-motor symptoms. This review aims to summarize the involvement of the globus pallidus in both motor and non-motor manifestations of Parkinson's disease. The firing activities of parvalbumin neurons in the medial globus pallidus, including both the firing rate and pattern, exhibit strong correlations with the bradykinesia and rigidity associated with Parkinson's disease. Increased beta oscillations, which are highly correlated with bradykinesia and rigidity, are regulated by the lateral globus pallidus. Furthermore,bradykinesia and rigidity are strongly linked to the loss of dopaminergic projections within the cortical-basal ganglia-thalamocortical loop. Resting tremors are attributed to the transmission of pathological signals from the basal ganglia through the motor cortex to the cerebellum-ventral intermediate nucleus circuit. The cortico–striato–pallidal loop is responsible for mediating pallidi-associated sleep disorders. Medication and deep brain stimulation are the primary therapeutic strategies addressing the globus pallidus in Parkinson's disease. Medication is the primary treatment for motor symptoms in the early stages of Parkinson's disease, while deep brain stimulation has been clinically proven to be effective in alleviating symptoms in patients with advanced Parkinson's disease,particularly for the movement disorders caused by levodopa. Deep brain stimulation targeting the globus pallidus internus can improve motor function in patients with tremordominant and non-tremor-dominant Parkinson's disease, while deep brain stimulation targeting the globus pallidus externus can alter the temporal pattern of neural activity throughout the basal ganglia–thalamus network. Therefore, the comp

关键词： anxiety basal ganglia bradykinesia deep brain stimulation depression globus pallidus externus globus pallidus internus lateral globus pallidus medial globus pallidus neural circuit Parkinson's disease

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Staged dendrite suppression for high safe and stable lithium-sulfur batteries

引用

Journal of Energy Chemistry 2025年第1期100卷 674-683页

作者： Jun Jiang Zhenjie Lu Yanwen Ding Shujun Liu Zhijie qi Tian Tang Yunfan Zhang Zhiyuan Ma Jingwen Sun Liang Xue Wenyao Zhang Pan Xiong xin Wang Junwu zhu Yongsheng Fu Key Laboratory for Soft Chemistry and Functional Materials of Ministry of Education Nanjing University of Science and Technology Jiangsu Pylon Battery Co.Ltd

The unavoidable dendrite growth and shuttle effect have long been stranglehold challenges limiting the safety and practicality of lithium-sulfur ***,we propose a dual-action strategy to address the lithium dendrite issue in stages by constructing a multifunctional surface-negatively-charged nanodiamond layer with high ductility and robust puncture resistance on polypropylene （PP） *** uniformly loaded compact negative layer can not only significantly enhance electron transmission efficiency and promote uniform lithium deposition,but also reduce the formation of dendrite during early deposition *** importantly,under the strong puncture stress encountered during the deterioration of lithium dendrite growth under limiting current,the high ductility and robust puncture resistance（145.88 MPa） of as-obtained nanodiamond layer can effectively prevent short circuits caused by unavoidable lithium *** Li||Li symmetrical cells assembled with nanodiamond layer modified PP demonstrated a stable cycle of over 1000 h at 2 mA cm-2with a polarization voltage of only 29.3 ***,the negative charged layer serves as a physical barrier blocking lithium polysulfide ions,effectively mitigating capacity *** improved cells achieved a capacity decay of only 0.042%per cycle after 700 cycles at 3 C,demonstrating effective suppression of dendrite growth and capacity attenuation,showing promising prospect.

关键词： Ductility

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

含Nb低温取向硅钢热轧板织构及析出物分析

引用

钢铁研究学报 2017年第10期29卷 823-830页

作者：刘彪宋新莉朱瑞琪贾涓武汉科技大学省部共建耐火材料与冶金国家重点实验室湖北武汉430081

分别采用电子背散射衍射技术(EBSD)、JEM-2100型透射电镜(TEM)研究了Nb元素对取向硅钢热轧板织构和析出物的影响。结果表明:取向硅钢添加Nb元素以后,热轧板中晶粒尺寸相对细小、Goss织构({110})含量更高、位向更精准,而且出现了传统取... 详细信息

分别采用电子背散射衍射技术(EBSD)、JEM-2100型透射电镜(TEM)研究了Nb元素对取向硅钢热轧板织构和析出物的影响。结果表明:取向硅钢添加Nb元素以后,热轧板中晶粒尺寸相对细小、Goss织构({110})含量更高、位向更精准,而且出现了传统取向硅钢热轧板中很难形成的γ取向线织构({111}和{111});透射电镜分析结果显示含Nb实验钢中析出相尺寸更细小而均匀,析出相除了MnS、AlN,还有NbCN,析出相含量增多,同时Nb的加入对细化其他析出物有积极的作用;含Nb取向硅钢中析出相的尺寸越小和体积分数越大,则析出相的抑制力也就越大,从而为形成的γ取向线织构和更高含量的Goss织构提供了有利的条件。

关键词：取向硅钢高斯织构析出物铌

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Cyclometalated Platinum (Ⅱ) Alkynyl Complexes with Bis(imidazoline) Ligands: Synthesis, Characterization and Photophysics

Cyclometalated Platinum (Ⅱ) Alkynyl Complexes with Bis(imid...

引用

河南省化学会2010年学术年会暨成立70周年庆祝会

作者： xin-Ju zhu,xin-qi Hao,Jun-Fang Gong,Mao-Ping Song(Department of Chemistry,Henan Key Laboratory of Chemical Biology and Organic Chemistry,Zhengzhou University,Zhengzhou 450052)

Since Hagihara [1] initially reported the synthesis and UV spectra of oligoyne polymers,the studies on transition metal σ-alkynyl complexes have begun to expand in the field of material ***,there has been an increasi...

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Visible-light-responsive homologous heterostructure of oxynitrides constructed to boost charge separation and water splitting

引用

Science China Chemistry 2025年第01期 186-191页

作者： Hai Zou Yunfeng Bao Xueshang xin Yu qi Tengfeng Xie Fuxiang Zhang State Key Laboratory of Catalysis Dalian National Laboratory for Clean Energy Dalian Institute of Chemical Physics Chinese Academy of Sciences School of Chemistry and Chemical Engineering Liaoning Normal University College of Chemistry Jilin University

Constructing type II heterostructures represents a widely recognized strategy for enhancing charge separation and photocatalytic efficiency,but it remains challenging to fabricate homologous heterostructures based on visible-light-responsive semiconductors,especially for popularly investigated （oxy）nitrides with low thermal ***,we synthesized a homologous heterostructure （denoted as YTON＿H） based on the oxynitrides of YTaO4-xNyand YTa *** heterostructure exhibits type II band structure alignment and intimate interface achieved through a magnesium （Mg） powder-assisted nitridation *** with widely reported heterologous heterostructures,the as-obtained YTON＿H homologous heterostructure could similarly exhibit enhanced charge separation as well as photocatalytic water splitting performance with respect to its counterparts(YTaO4-xNyand YTaON2).Meanwhile,detailed structural characterizations and spectroscopy analysis have been made to confirm the formation of heterostructure and promotion of spatial charge separation.

关键词：

来源：

同方期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Differential distribution of PINK1 and Parkin in the primate brain implies distinct roles

引用

Neural Regeneration Research 2025年第4期20卷 1124-1134页

作者： Yanting Liu Wei Huang Jiayi Wen xin Xiong Ting Xu qi Wang Xiusheng Chen Xianxian Zhao Shihua Li Xiaojiang Li Weili Yang Guangdong Key Laboratory of Non-human Primate Research Key Laboratory of CNS Regeneration(Ministry of Education)Guangdong-Hongkong-Macao CNS Regeneration Institute of Jinan UniversityJinan UniversityGuangzhouGuangdong ProvinceChina

The vast majority of in vitro studies have demonstrated that PINK1 phosphorylates Parkin to work together in mitophagy to protect against neuronal ***,it remains largely unclear how PINK1 and Parkin are expressed in mammalian *** has been difficult to address because of the intrinsically low levels of PINK1 and undetectable levels of phosphorylated Parkin in small *** this issue is critical for elucidating the in vivo roles of PINK1 and ***,we showed that the PINK1 kinase is selectively expressed as a truncated form(PINK1–55)in the primate *** the present study,we used multiple antibodies,including our recently developed monoclonal anti-PINK1,to validate the selective expression of PINK1 in the primate *** found that PINK1 was stably expressed in the monkey brain at postnatal and adulthood stages,which is consistent with the findings that depleting PINK1 can cause neuronal loss in developing and adult monkey ***1 was enriched in the membrane-bound fractionations,whereas Parkin was soluble with a distinguishable *** double staining experiments showed that PINK1 and Parkin did not colocalize under physiological conditions in cultured monkey astrocytes,though they did colocalize on mitochondria when the cells were exposed to mitochondrial *** findings suggest that PINK1 and Parkin may have distinct roles beyond their well-known function in mitophagy during mitochondrial damage.

关键词： neurodegeneration Parkin Parkinson’s disease PINK1 subcellular distribution

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：