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Polypyrimidine Tract-Binding Protein Enhances Zika Virus Translation by Binding to the 5'UTR of Internal Ribosomal Entry Site

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Chinese Medical Sciences Journal 2024年第3期39卷 163-172页

作者： Moliduer Hamiti xin-tian zhang Rui-Min Zhu Yun-Peng Liu Bin Yin Peng-Cheng Shu Xiao-Zhong Peng State Key Laboratory of Common Mechanism Research for Major Diseases Department of Molecular Biology and BiochemistryMedical Primate Research CenterNeuroscience CenterInstitute of Basic Medical Sciences Chinese Academy of Medical Sciences&School of Basic Medicine Peking Union Medical CollegeBeijing 100005China institute of Medical Biology Chinese Academy of Medical Sciences&Peking Union Medical CollegeKunming 650031China State Key Laboratory of Respiratory Health and Multimorbidity Chinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100021China lnstitute of Laboratory Animal Science Chinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100021China

Objectives To identify the 5'untranslated region of Zika virus(ZIKV 5'UTR)RNA-binding proteins and to investigate the impact of the binding protein on the activity of internal ribosomal entry site(IRES)located in ZIKV 5'UTR and virus *** Interacting proteins in U251 cells were captured using tRSA-tagged ZIKV 5'UTR RNA and tRSA-ZIKV 5'UTR RNA-binding proteins were visualized by SDS-PAGE silver staining,Subsequently,liquid chromatographytandem mass spectrometry(LC-MS/MS),bioinformatics analysis,and Western blot were used to identify the candidate proteins binding to ZIKV 5'*** expression assay and plaque forming assay were performed to analyze the effect of the binding protein on ZIKV IRES activity and ZIKV production,*** tRSA RNA pull-down assay,LC-MS/MS,and Western blot analysis showed that polypyrimidine tractbinding protein(PTB)bound to the ZIKV 5'***,dual luciferase reporter assay revealed that overexpression of PTB significantly enhanced the IRES activity of ZIKV(t=10.220,P<0.001),while PTB knockdown had the opposite effect(t=4.897,P<0.01).Additionally,virus plaque forming assay demonstrated that up-regulation of PTB expression significantly enhanced viral titer(t=6.400,P<0.01),whereas reducing PTB expression level weakened virus infectivity(t=5.055,P<0.01).Conclusion PTB positively interacts with the ZIKV 5'UTR and enhances IRES activity and virus production.

关键词： internal ribosomal entry site polypyrimidine tract-binding protein Zika virus tRSA RNA pull-down dual-luciferase reporter assay

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Facile Synthesis of Polyisothioureas via Alternating Copolymerization of Aziridines and Isothiocayanates

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Chinese Journal of Polymer Science 2024年第3期42卷 292-298,I0005页

作者： Hao-tian zhang Ming-xin Niu Qi zhang Chen-Yang Hu Xuan Pang Key Laboratory of Polymer Ecomaterials Changchun Institute of Applied ChemistryChinese Academy of SciencesChangchun 130022China Department of Materials Science and Engineering Jilin UniversityChangchun 130025China School of Applied Chemistry and Engineering University of Science and Technology of ChinaHefei 230026China

Isothiourea is an important class of sulfur-containing molecules showing unique catalytic and biological activities. As such,polyisothiourea is envisioned to be an interesting type of polymer that potentially exhibits a number of interesting properties. However, there is no access to synthesizing well-defined polyisothiourea, and currently isothiourea-containing polymers are mainly prepared by immobilizing onto other polymer's side chain. Herein, we report the first facile synthesis of polyisothioureas via alternating copolymerization of aziridines and isothiocayanates. Mediated by the catalytic system of phosphazene superbases/alcohol, a broad scope of aziridines and isothiocayanates could be transformed into polyisothioureas with adjustable substitutions(11 examples). The structures of obtained polyisothioureas were fully characterized with ^(1)H-NMR, ^(13)C-NMR, and ^(1)H-^(13)C HMBC NMR. Moreover, the polyisothioureas show tunable thermal properties depending on substitutions on the isothiourea linkages. The novel structure of these polyisothioureas will enable a powerful platform for the discovery of nextgeneration functional plastics.

关键词： Polyisothioureas Alternative copolymer Aziridines Isothiocayanates

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Influence of friction-lubrication characteristics varying with rolling speed on instability of chatter and slip in cold tandem rolling process

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Journal of Iron and Steel Research International 2024年第4期31卷 894-908页

作者： Yan-li xin Zhi-ying Gao Bo tian Qing-dong zhang School of Mechanical Engineering University of Science and Technology BeijingBeijing 100083China

In the high-speed cold tandem rolling process of thin plate,chatter or slip instability gives rise to the deterioration of equipment and product *** instability behavior is closely related to interfacial friction-lubrication con-dition which is generally characterized by the friction ***,with higher and higher speed requirements,the commonly used model of friction coefficient is no longer applicable and accurate.A novel approach is suggested to calculate the speed-dependent friction coefficient,in which the viscosity-pressure-temperature effects of the lubricant,surface roughness states of work rolls and rolled piece are comprehensively involved and the mixed flm lubrication theory is ***,the influences of friction coefficient on the instability of slip and chatter are investigated for a five-stand cold tandem rolling *** one side,the critical friction coefficient for each stand is determined by calculating the corresponding slip factor;on the other hand,the friction coefficient varying with rolling speed is combined with the model of theoretical critical rolling speed presented under constant friction coefficient,so that the speed threshold is ***,the stable and unstable regions corresponding to the rear three stands are individually discussed,and the technical strategies are proposed to suppress both slip and chatter frequently occurring in the actual rolling process.

关键词： Cold tandem rolling:Oil film thickness Speed-dependent friction coefficient-Slip:Chatter

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More Than Lightening:A Self-Supervised Low-Light Image Enhancement Method Capable for Multiple Degradations

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IEEE/CAA Journal of Automatica Sinica 2024年第3期11卷 622-637页

作者： Han Xu Jiayi Ma Yixuan Yuan Hao zhang xin tian Xiaojie Guo the Electronic Information School Wuhan UniversityWuhan 430072China IEEE the Department of Electronic Engineering Chinese University of Hong KongHong Kong 999077China the College of Intelligence and Computing Tianjin UniversityTianjin 300350China

Low-light images suffer from low quality due to poor lighting conditions,noise pollution,and improper settings of *** enhance low-light images,most existing methods rely on normal-light images for guidance but the collection of suitable normal-light images is *** contrast,a self-supervised method breaks free from the reliance on normal-light data,resulting in more convenience and better *** self-supervised methods primarily focus on illumination adjustment and design pixel-based adjustment methods,resulting in remnants of other degradations,uneven brightness and *** response,this paper proposes a self-supervised enhancement method,termed as *** can handle multiple degradations including illumination attenuation,noise pollution,and color shift,all in a self-supervised *** attenuation is estimated based on physical principles and local neighborhood *** removal and correction of noise and color shift removal are solely realized with noisy images and images with color ***,the comprehensive and fully self-supervised approach can achieve better adaptability and *** is applicable to various low light conditions,and can reproduce the original color of scenes in natural *** experiments conducted on four public datasets demonstrate the superiority of SLIE to thirteen state-of-the-art *** code is available at https://***/hanna-xu/SLIE.

关键词： Color correction low-light image enhancement self-supervised learning.

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Effect of Al content in molten steel on interaction between MgO-C refractory and SPHC steel

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Journal of Iron and Steel Research International 2024年第4期31卷 838-848页

作者： Fu-bin Gao Fu-ming Wang Xiang zhang tian-yu Du xin-hua Wangg School of Metallurgical and Ecological Engineering University of Science and Technology BeijingBeijing 100083China Handan Iron and Steel Company Hegang GroupHandan 056015HebeiChina The State Key Laboratory of Refractories and Metallurgy Wuhan University of Science and TechnologyWuhan 430081HubeiChina

The effect of Al content in molten steel on the interaction between SPHC steel(0.005-0.068 wt.%Al,and 19×10^(-4)-58×10^(-4)wt.%O)and MgO-C refractory(11.63 wt.%C)was ***-metallic inclusions in the steel were examined at various periods(0,5,15,30,45,and 60 min)as well as the MgO-C interface after 60 min of corrosion at 1600℃.The results show that when MgO-C refractory comes into contact with SPHC steel,the refractory interface consists of three layers arranged from the innermost to the outermost,including the original refractory layer,the dense MgO layer,and the iron infiltration *** carbon in the MgO-C refractories and the Al content in the molten steel undergo a reaction with the MgO in the refractories,resulting in an increase in Mg concentration in the *** Al content in the molten steel from 0.005 to 0.068 wt.%causes a spinel layer to appear at the interface,and the disappearance time of Al_(2)O_(3) inclusions in the steel decreases from 60 to 30 min,while the average MgO content in inclusions ***,controlling the Al content in the molten steel and the smelting duration can help regulate the formation of spinel inclusionsinthe steel.

关键词： MgO-C refractory SPHC steel Inclusion-Spinel Al-killed steel Interfacial reaction

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Construction of RuO2-Ru/MoO2@carbon cloth bifunctional electrocatalyst for efficient overall water splitting

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Rare Metals 2024年第10期43卷 5095-5104页

作者： Jia-Lin Cai Jing-Yi Fan Xu-Dong zhang xin Xie Wan-Yu tian xin-Gang zhang Jie Ding Yu-Shan Liu College of Material Henan Key Laboratory of Aeronautical Materials and Application TechnologyZhengzhou University of Aeronautics The Green Catalysis Center and College of ChemistryZhengzhou University

Efficient bifunction electrocatalyst is extremely interesting for electrochemical overall water splitting （OWS）.Herein,a new RuO2-Ru/MoO2@CC（RRM/CC） bifunctional electrocatalyst was prepared via a solid phase reaction *** obtain a suitable precursor for SPR,MoS2nanosheets and RuO2nanoparticles （NPs）were sequentially loaded onto carbon cloth conductive ***,the prepared RuO2/MoS2/CC precursor was sealed in a furnace and annealed in Ar to trigger the redox *** SPR,active RuO2-Ru/MoO2units containing metal–metal oxide interfaces were formed on CC substrate *** optimized RRM/CC sample annealed at 400℃ exhibited a overpotential of 13 m V for hydrogen evolution reaction （HER） and 231 mV for oxygen evolution reaction （OER） at 10 mA·cm-2under alkaline condition,respectively,which can be deduced to the modulated electronic structure and unique hierarchica *** addition,a low cell voltage of 1.48 V for OWS was required at 10 mA·cm-2under alkaline condition Meanwhile,RRM/CC exhibited excellent pH-independent durability.

关键词： Bifunctional Electrocatalyst Overall water splitting Ru RuO2

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Deformation of Rigid Conjugate Self-dual Galois Representations

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Acta Mathematica Sinica,English Series 2024年第7期40卷 1599-1644页

作者： Yi Feng LIU Yi Chao tian Liang XIAO Wei zhang xin Wen ZHU Institute for Advanced Study in Mathematics Zhejiang UniversityHangzhou 310058P.R.China Morningside Center of Mathematics AMSSChinese Academy of SciencesBeijing 100190P.R.China New Cornerstone Lab Beijing International Center for Mathematical ResearchPeking Ui Beijing 100871P.R.China Department of Mathematics Massachusetts Institute of TechnologyCambridge MA 02139United States Department of Mathematics Stanford UniversityStanfordCA 94305United States

In this article,we study deformations of conjugate self-dual Galois *** study is ***,we prove an R=T type theorem for a conjugate self-dual Galois representation with coefficients in a finite field,satisfying a certain property called ***,we study the rigidity property for the family of residue Galois representations attached to a symmetric power of an elliptic curve,as well as to a regular algebraic conjugate self-dual cuspidal representation.

关键词： Galois deformation rigid conjugate self-dual Galois representations

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Robust adaptive radar beamforming based on iterative training sample selection using kurtosis of generalized inner product statistics

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Journal of Systems Engineering and Electronics 2024年第1期35卷 24-30页

作者： tian Jing zhang Wei School of Information and Electronics Beijing Institute of TechnologyBeijing 100081China National Key Laboratory of Electromagnetic Space Security Chengdu 610036China

In engineering application,there is only one adaptive weights estimated by most of traditional early warning radars for adaptive interference suppression in a pulse reputation interval(PRI).Therefore,if the training samples used to calculate the weight vector does not contain the jamming,then the jamming cannot be removed by adaptive spatial *** the weight vector is constantly updated in the range dimension,the training data may contain target echo signals,resulting in signal cancellation *** cope with the situation that the training samples are contaminated by target signal,an iterative training sample selection method based on non-homogeneous detector(NHD)is proposed in this paper for updating the weight vector in entire range *** principle is presented,and the validity is proven by simulation results.

关键词： adaptive radar beamforming training sample selection non-homogeneous detector electronic jamming jamming suppression

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Photoinduced copper-catalyzed alkoxyl radical-triggered ring-expansion/aminocarbonylation cascade

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Chinese Chemical Letters 2024年第6期35卷 331-336页

作者： Jing-Qi Tao Shuai Liu tian-Yu zhang Hong xin Xu Yang xin-Hua Duan Li-Na Guo Department of Chemistry School of ChemistryXi’an Key Laboratory of Sustainable Energy Material Chemistry and Engineering Research Center of Energy Storage Materials and DevicesMinistry of EducationXi’an Jiaotong UniversityXi’an 710049China School of Electrical Engineering Xi’an Jiaotong UniversityXi’an 710049China State Key Laboratory of Applied Organic Chemistry Lanzhou UniversityLanzhou 730000China

A photoinduced copper-catalyzed alkoxyl triggered C-C bond cleavage/aminocarbonylation cascade is *** adjusting the structure of alkoxyl radical precursors,functionalized lactones and ketoamides were synthesized with good yields and excellent functional group tolerance under redox-neutral ***,this protocol enables the integration of lactone fragments with many amine drugs and drug fragments.

关键词： Copper catalysis Ring-expansion Aminocarbonylatioin Macrolactones Keto-amides

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Frequency Domain Fatigue Evaluation on SCR Girth-Weld Based on Structural Stress

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China Ocean Engineering 2024年第2期38卷 255-270页

作者： zhang Long ZHAO tian-feng College of Safety and Ocean Engineering China University of Petroleum(Beijing)Beijing 102249China

The Steel Catenary Riser(SCR)is a vital component for transporting oil and gas from the seabed to the floating *** harsh environmental conditions and complex platform motion make the SCR’s girth-weld prone to fatigue *** structural stress fatigue theory and Master S-N curve method provide accurate predictions for the fatigue damage on the welded joints,which demonstrate significant potential and compatibility in multi-axial and random fatigue ***,we propose a new frequency fatigue model subjected to welded joints of SCR under multiaxial stress,which fully integrates the mesh-insensitive structural stress and frequency domain random process and transforms the conventional welding fatigue technique of SCR into a spectrum analysis technique utilizing structural ***,a full-scale FE model of SCR with welds is established to obtain the modal structural stress of the girth weld and the frequency response function(FRF)of modal coordinate,and a biaxial fatigue evaluation about the girth weld of the SCR can be achieved by taking the effects of multi-load correlation and pipe-soil interaction into *** research results indicate that the frequency-domain fatigue results are aligned with the time-domain results,meeting the fatigue evaluation requirements of the SCR.

关键词： SCR girth weld random vibration self(cross)power spectrum structural stress method biaxial fatigue damage

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