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Differential distribution of PINK1 and Parkin in the primate brain implies distinct roles

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Neural Regeneration Research 2025年第4期20卷 1124-1134页

作者： yanting Liu Wei Huang Jiayi Wen xin Xiong Ting Xu Qi Wang Xiusheng Chen Xianxian Zhao Shihua Li Xiaojiang Li Weili yang Guangdong Key Laboratory of Non-human Primate Research Key Laboratory of CNS Regeneration(Ministry of Education)Guangdong-Hongkong-Macao CNS Regeneration Institute of Jinan UniversityJinan UniversityGuangzhouGuangdong ProvinceChina

The vast majority of in vitro studies have demonstrated that PINK1 phosphorylates Parkin to work together in mitophagy to protect against neuronal ***,it remains largely unclear how PINK1 and Parkin are expressed in mammalian *** has been difficult to address because of the intrinsically low levels of PINK1 and undetectable levels of phosphorylated Parkin in small *** this issue is critical for elucidating the in vivo roles of PINK1 and ***,we showed that the PINK1 kinase is selectively expressed as a truncated form(PINK1–55)in the primate *** the present study,we used multiple antibodies,including our recently developed monoclonal anti-PINK1,to validate the selective expression of PINK1 in the primate *** found that PINK1 was stably expressed in the monkey brain at postnatal and adulthood stages,which is consistent with the findings that depleting PINK1 can cause neuronal loss in developing and adult monkey ***1 was enriched in the membrane-bound fractionations,whereas Parkin was soluble with a distinguishable *** double staining experiments showed that PINK1 and Parkin did not colocalize under physiological conditions in cultured monkey astrocytes,though they did colocalize on mitochondria when the cells were exposed to mitochondrial *** findings suggest that PINK1 and Parkin may have distinct roles beyond their well-known function in mitophagy during mitochondrial damage.

关键词： neurodegeneration Parkin Parkinson’s disease PINK1 subcellular distribution

A novel deep generative model for mRNA vaccine development: Designing 5′ UTRs with N1-methyl-pseudouridine modification

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Acta Pharmaceutica Sinica B 2024年第4期14卷 1814-1826页

作者： Xiaoshan Tang Miaozhe Huo yuting Chen Hai Huang Shugang Qin Jiaqi Luo Zeyi Qin xin Jiang yongmei Liu xing Duan Ruohan Wang Lingxi Chen Hao Li Na Fan Zhongshan He Xi He Bairong Shen Shuai Cheng Li Xiangrong Song Institute of Systems Genetics Department of Critical Care MedicineFrontiers Science Center for Disease-related Molecular NetworkState Key Laboratory of Biotherapy and Cancer CenterWest China HospitalSichuan UniversityChengdu 610000China Department of Computer Science City University of Hong KongHong Kong 99907China Department of Biology Brandeis UniversityBostonMA 02453USA

Efficient translation mediated by the 5'untranslated region(5'UTR)is essential for the robust efficacy of mRNA ***,the N1-methyl-pseudouridine(m1)modification of mRNA can impact the translation efficiency of the 5'*** discovered that the optimal 5'UTR for m1y-modified mRNA(m1y-5'UTR)differs significantly from its unmodified counterpart,high-lighting the need for a specialized tool for designing mly-5'UTRs rather than directly utilizing high-expression endogenous gene 5'*** response,we developed a novel machine learning-based tool,Smart5UTR,which employs a deep generative model to identify superior m1y-5'UTRs in *** tailored loss function and network architecture enable Smart5UTR to overcome limitations inherent in existing *** a result,Smart5UTR can successfully design superior 5'UTRs,greatly benefiting mRNA vaccine ***,Smart5UTR-designed superior 5'UTRs significantly enhanced antibody titers induced by COVID-19 mRNA vaccines against the Delta and Omicron variants of SARS-CoV-2,surpassing the performance of vaccines using high-expression endogenous gene 5'UTRs.

关键词： mRNA vaccine Machine learning 5'UTR mRNA design Sequence design N1-Methyl-pseudouridine COVID-19 SARS-CoV-2

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二元模糊关系范畴的对称幺半范畴性

云南大学学报（自然科学版） 2024年第2期46卷 219-227页

作者：周鑫刘淼伊犁师范大学数学与统计学院新疆伊宁835000 伊犁师范大学应用数学研究所新疆伊宁835000

结合模糊关系范畴的概念给出了二元模糊关系范畴L_(b)Rel的概念.首先,讨论了范畴L_(b)Rel的积和余积的结构.其次,定义了张量积函子■,得到了L_(b)Rel是对称幺半范畴.进而,给出了范畴L_(b)Rel中的幺半群和余幺半群结构.最后,以二元模糊... 详细信息

结合模糊关系范畴的概念给出了二元模糊关系范畴L_(b)Rel的概念.首先,讨论了范畴L_(b)Rel的积和余积的结构.其次,定义了张量积函子■,得到了L_(b)Rel是对称幺半范畴.进而,给出了范畴L_(b)Rel中的幺半群和余幺半群结构.最后,以二元模糊关系范畴L_(b)Rel作为纽带,构造了一个从模糊集范畴LSet到模糊关系范畴RelLL的忠实函子.

关键词：模糊关系二元模糊关系范畴函子对称幺半范畴

Coercivity mechanism of La-Nd-Fe-B films with y spacer layer

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Chinese Physics B 2024年第3期33卷 138-143页

作者：马俊赵晓天刘伟李阳刘龙赵新国张志东 Shenyang National Laboratory for Materials Science Institute of Metal ResearchChinese Academy of SciencesShenyang 110016China School of Materials Science and Engineering University of Science and Technology of ChinaShenyang 110016China

The effect of the y spacer layer on the phase composition,coercivity.and magnetization reversal processes of La-Nd-Fe-B films has been *** addition of a 10 nm y spacer layer increases the coercivity of the film to 1.36 T at 300 K and remains 0.938 T at 380 *** the thickness of the y spacer layer increases,y participates in the formation of the main phase in the film,and further regulates the formation of La-B *** results of the first-order reversal curve(FORC)and micromagnetic fitting show that the coercivity of all the films is dominated by nucleation *** c-axis preferred orientation,good magnetic microstructure parameters and the largest dipole interaction enhance the ***,the introduction of the y spacer layer can be an effective way to improve the coercivity of La-Nd-Fe-B film over a wide temperature range of 150 K-380 K.

关键词： La-Nd-(y)-Fe-B films magnetization reversal mechanisms coercivity multilayers

一种复杂地形场景点云的WOA-CSF自适应性滤波方法

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激光与红外 2024年第5期54卷 725-733页

作者：戚鑫鑫王磊储栋池深深安徽理工大学空间信息与测绘工程学院安徽淮南232001 安徽理工大学矿山采动灾害空天地协同监测与预警安徽普通高校重点实验室安徽淮南232001 安徽理工大学矿区环境与灾害协同监测煤炭行业工程研究中心安徽淮南232001

为解决布料模拟滤波算法(CSF)自适应性不高的问题,提出了一种基于鲸鱼优化算法(WOA)和自适应参数调整的改进CSF算法(WOA-CSF)。本文首先构造以误分类点云误差率最小为标准的适应度评价函数,然后采用WOA算法对CSF算法的四个参数进行自适... 详细信息

为解决布料模拟滤波算法(CSF)自适应性不高的问题,提出了一种基于鲸鱼优化算法(WOA)和自适应参数调整的改进CSF算法(WOA-CSF)。本文首先构造以误分类点云误差率最小为标准的适应度评价函数,然后采用WOA算法对CSF算法的四个参数进行自适应寻优,构建了WOA-CSF滤波算法,最后开展了WOA-CSF算法与CSF算法滤波实验的对比研究。实验结果表明:WOA-CSF算法在城市、乡镇、村庄和山区等四种复杂环境下平均Kappa系数从68.33%提升到81.54%,平均总误差率从10.54%下降到6.62%,平均I类误差率从25.87%下降到6.77%,在复杂场景下较好地滤除非地面点的同时,又极大程度上保留了地形特征。

关键词：复杂地形场景布料模拟滤波鲸鱼优化算法点云滤波智能优化

Finite deformation analysis of the rotating cylindrical hollow disk composed of functionally-graded incompressible hyper-elastic material

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Applied Mathematics and Mechanics(English Edition) 2023年第8期44卷 1367-1384页

作者： Libiao xin yang WANG Zhiqiang LI y.B.LI College of Mechanical and Vehicle Engineering Taiyuan University of TechnologyTaiyuan 030024China College of Aeronautics and Astronautics Taiyuan University of TechnologyTaiyuan 030024China Department of Mechanical and Aerospace Engineering North Carolina State UniversityRaleighNC 27606U.S.A.

The deformations and stresses of a rotating cylindrical hollow disk made of incompressible functionally-graded hyper-elastic material are theoretically.analyzed based on the finite elasticity *** hyper-elastic material is described by a new micro-macro transition ***,the material shear modulus and density are assumed to be a function with a power law form through the radial direction,while the material inhomogeneity is thus reflected on the power index *** integral forms of the stretches and stress components are *** the obtained complicated integral forms,the composite trapezoidal rule is utilized to derive the analytical solutions,and the explicit solutions for both the stretches and the stress components are numerically *** comparing the results with two classic models,the superiority of the model in our work is ***,the distributions of the stretches and normalized stress components are discussed in detail under the effects of *** results indicate that the material inhomogeneity.and the rotating angular velocity have significant effects on the distributions of the normalized radial and hoop stress components and the *** believe that by appropriately choosing the material inhomogeneity.and configuration parameters,the functionally-graded material(FGM)hyper-elastic hollow cylindrical disk can be designed to meet some unique requirements in the application fields,e.g.,soft robotics,medical devices,and conventional aerospace and mechanical industries.

关键词： functionally-graded material(FGM) incompressible hyper-elastic model rotating hollow cylindrical disk

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甲基苯丙胺成瘾的表观遗传学机制

生物化学与生物物理进展 2024年第4期51卷 873-880页

作者：刘明鑫司紫珍刘昱宁波大学医学部宁波315211

苯丙胺类兴奋剂是全世界第二大滥用程度的药物,甲基苯丙胺作为苯胺类兴奋剂中的主要药物,是中国滥用的“头号毒品”。而现有的研究对甲基苯丙胺成瘾机制尚不清晰,且临床上对药物成瘾的治疗依然存在无药可医的局面。因此,发现新的成瘾机... 详细信息

苯丙胺类兴奋剂是全世界第二大滥用程度的药物,甲基苯丙胺作为苯胺类兴奋剂中的主要药物,是中国滥用的“头号毒品”。而现有的研究对甲基苯丙胺成瘾机制尚不清晰,且临床上对药物成瘾的治疗依然存在无药可医的局面。因此,发现新的成瘾机制和治疗策略尤为迫切。甲基苯丙胺成瘾与额前叶皮质(mPFC)、中脑腹侧被盖区(VTA)和伏隔核(NAc)中的多巴胺(DA)、谷氨酸(Glu)、去甲肾上腺素(NE)和血清素(SNRIS)等神经递质的异常释放有关。研究表明,这些神经递质受到表观遗传机制中组蛋白乙酰化、甲基化、泛素化和非编码RNA等调节,某些基因的表达在甲基苯丙胺的诱导过程中增强或被抑制,导致甲基苯丙胺依赖性产生。本文将针对表观遗传学对甲基苯丙胺成瘾机制的影响进行着重论述,以期推进临床开发甲基苯丙胺戒断药物的研究。

关键词：甲基苯丙胺表观遗传学组蛋白修饰非编码RNA

针刺治疗中风后中枢性疼痛的系统评价与Meta分析

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Journal of Acupuncture and Tuina Science 2024年第4期22卷 341-352页

作者：程鑫妮张欣纪军 Shanghai University of Traditional Chinese Medicine Shanghai 201203China Shanghai Research Institute of Acupuncture and Meridian Shanghai 200030China

目的:评价针刺治疗中风后中枢性疼痛(CPSP)的有效性和安全性。方法:计算机检索PubMed、EMBASE、Cochrane Library、中国知网(CNKI)、万方数据知识服务平台(Wanfang)、维普资讯中文期刊服务平台(VIP)、中国生物医学文献数据库(CBM)中针... 详细信息

目的:评价针刺治疗中风后中枢性疼痛(CPSP)的有效性和安全性。方法:计算机检索PubMed、EMBASE、Cochrane Library、中国知网(CNKI)、万方数据知识服务平台(Wanfang)、维普资讯中文期刊服务平台(VIP)、中国生物医学文献数据库(CBM)中针刺治疗CPSP的随机对照试验文献。检索时间从建库至2023年7月。采用RevMan5.3软件进行meta分析;GRADEprofiler3.6.1软件进行证据质量评价。二分类资料采用相对危险度(RR)进行分析。连续性资料采用均数差(MD)进行分析,置信区间(CI)取95%进行计算。结果:纳入14篇文献,共1045例患者。Meta分析结果表明,与西药治疗CPSP相比,针刺治疗的临床有效率更高[RR=1.09,95%CI(1.01,1.19),Z=2.08,P0.05]。与西药治疗CPSP比较,针刺+西药治疗的临床有效率更高[RR=1.18,95%CI(1.05,1.34),Z=2.75,P<0.05]、VAS评分更低[MD=-1.04,95%CI(-1.26,-0.82),Z=9.25,P<0.001]、匹兹堡睡眠质量指数(PSQI)评分更低[MD=-2.67,95%CI(-4.80,-0.54),Z=2.46,P<0.05]。证据质量等级评价结果显示针刺或针刺+西药与西药对比治疗CPSP均无中、高质量的证据。结论:针刺治疗CPSP较西药治疗有一定的疗效优势,能够有效缓解疼痛,改善睡眠,且出现不良反应较少,安全性较高,但仍需高质量的随机对照试验进一步研究与验证。

关键词：针刺疗法中风中风后遗症中枢性疼痛 Meta分析系统评价

Multiomics analyses of two Leonurus species illuminate leonurine biosynthesis and its evolution

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Molecular Plant 2024年第1期17卷 158-177页

作者： Peng Li Meng-Xiao yan Pan Liu Dan-Jie yang Ze-Kun He yun Gao yan Jiang yu Kong xin Zhong Sheng Wu Jun yang Hong-Xia Wang yan-Bo Huang Le Wang Xiao-ya Chen yong-Hong Hu Qing Zhao Ping Xu shanghai Key Laboratory of Plant Functional Genomics and Resources Shanghai Chenshan Botanical GardenShanghai Chenshan Plant Science Research CenterChineseAcademy of SciencesShanghaiChina College of Life Sciences Shanghai Normal UniversityShanghai 200234China State Key Laboratory of Plant Molecular Genetics CAS Center for Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghaiChina University of the Chinese Academy of Sciences Beijing 100049China The Research Center of Chiral Drugs Innovation Research Institute of Traditional Chinese MedicineShanghai University of Traditional Chinese Medicine1200 Cailun RoadShanghai 201203China College of Chemistry.and Chemical Engineering Shanghai University of Engineering ScienceShanghai 201620China

The Lamiaceae family is renowned for its terpenoid-based medicinal components,but Leonurus,which has traditional medicinal uses,stands out for its alkaloid-rich ***,the principal active compound found in Leonurus,has demonstrated promising effects in reducing blood lipids and treating ***,the biosynthetic pathway of leonurine remains largely ***,we present the chromosome-level genome sequence assemblies of Leonurus japonicus,known for its high leonurine production,and Leonurus sibiricus,characterized by very limited leonurine *** integrating genomics,RNA sequencing,metabolomics,and enzyme activity assay data,we constructed the leonurine biosynthesis pathway.and identified the arginine decarboxylase(ADC),uridine diphosphate glucosyltransferase(UGT),and serine carboxypeptidase-like(SCPL)acyltransferase enzymes that catalyze key reactions in this *** analyses revealed that the UGT–SCPL gene cluster evolved by gene duplication in the ancestor of Leonurus and neofunctionalization of SCPL in ***,which contributed to the accumulation of leonurine specifically in ***,our comprehensive study illuminates leonurine biosynthesis and its evolution in Leonurus.

关键词： Lamiaceae Leonurus japonicus Leonurus sibiricus genome sequence alkaloids leonurine gene cluster biosynthetic pathway evolution