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Biomedical and Environmental Sciences 2002年第1期15卷 1-7页

作者： ZHU xin-PING, ZHANG xin-MEI, ZHOU lei,YANG YA-PING, AND GAO xinDepartment of Parasitology, Capital University of MedicalSciences, Beijing 100054, China Department of Parasitology Capital University of Medical Sciences Beijing 100054 China

Objective To sequence the gene encoding glutamate rich protein (GLURP) andidentify the genotypes of geographically different Plasmodium falciparum (P. f ) isolatesfrom China. Methods The gene of R2 repeat region of GLURP was amplified by nestedpolymerase chain reaction and cloned into T-vector. The nucleotide sequence of GLURPgene was determined by automatic sequencer (Dideoxy termination method) and analyzed byDNA Star software. Results At least 7 different GLURP genotypes ranging from 600 bpto 1 500 bp were found in Yunnan and Hainan provinces. R2 region of GLURP gene consistedof several repeat units. Each repeat unit was composed of 19-20 residues which were shownto be highly conserved. GLURP gene was also size polymorphic due to differences in thenumber of repeat units, whereas the repeat sequence was conserved. Sequence analysisshowed that DNA sequences and deduced amino acid sequences were highly homologousamong the geographically dispersed isolates or various isolates from the same geographicalregion. No obvious differences were found in the GLURP gene sequences amonggeographically different isolates. Conclusion GLURP gene is highly structure conservedand size polymorphic, and so is useful in searching for malaria vaccine candidate antigen anddeveloping a genotyping method for malaria research.

关键词： GLURP Plasmodium falciparum Malaria

Impact of sympatric carnivores on den selection of wild giant pandas

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Zoological Research 2020年第3期41卷 273-280页

作者： xin-lei Lai Wen-Liang Zhou Hua-lei Gao Meng Wang Kai Gao Bao-Wei Zhang Fu-Wen Wei Yong-Gang Nie School of Life Sciences Anhui UniversityHefeiAnhui 230601China Key Laboratory of Animal Ecology and Conservation Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering College of Life SciencesBeijing Normal UniversityBeijing 100875China Center for Excellence in Animal Evolution and Genetics Chinese Academy of SciencesKunmingYunnan 650223China

Interspecific killing is a primary reason for the low survival rates of some animal species. The giant panda(Ailuropoda melanoleuca) is an altricial eutherian mammal and thus, in comparison to other infants, panda cubs are highly vulnerable, which may significantly influence the selection of breeding sites by females. Here, we used infrared camera traps to monitor giant panda dens for 5.5 years in Foping National Nature Reserve(FNNR) to determine how interspecific factors affect den selection by wild female pandas. Results indicated that Asian black bears(Ursus thibetanus), yellow-throated martens(Martes flavigula), leopard cats(Prionailurus bengalensis), and masked palm civets(Paguma larvata) visited the dens frequently, and the presence of these species negatively influenced den selection by female pandas. Interestingly, the presence of rodents and terrestrial birds appeared to indicate den safety, and female giant pandas were not averse and even preferred dens with a high abundance index of rodents and terrestrial birds. The den suitability index(DSI) was a reliable tool for evaluating whether dens were suitable for female giant pandas to give birth to and rear cubs, with preference for dens with high DSI values. This study increases our understanding of the den selection criteria of female giant pandas and the main threats to the survival of their cubs,thus providing important guidance for the conservation and management of this species.

关键词： Ailuropoda melanoleuca Interspecific interference competition Dens Den suitability index

Study on the controllability of the fabrication of single-crystal silicon nanopores/nanoslits with a fast-stop ionic current-monitored TSWE method

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Microsystems & Nanoengineering 2023年第3期9卷 51-60页

作者： Hao Hong Jiangtao Wei xin lei Haiyun Chen Pasqualina M.Sarro Guoqi Zhang Zewen Liu Department of Microelectronics Delft University of Technology2628 CD DelftThe Netherlands School of Integrated Circuits Tsinghua University100084 BeijingChina School of Chemistry Beihang University100084 BeijingChina School of Electronic and Information Engineering Beijing Jiaotong University100084 BeijingChina

The application of single-crystal silicon(SCS)nanopore structures in single-molecule-based analytical devices is an emerging approach for the separation and analysis of *** key challenge is to fabricate individual SCS nanopores with precise sizes in a controllable and reproducible *** paper introduces a fast-stop ionic current-monitored three-step wet etching(TSWE)method for the controllable fabrication of SCS *** the nanopore size has a quantitative relationship with the corresponding ionic current,it can be regulated by controlling the ionic *** to the precise current-monitored and self-stop system,an array of nanoslits with a feature size of only 3 nm was obtained,which is the smallest size ever reported using the TSWE ***,by selecting different current jump ratios,individual nanopores of specific sizes were controllably prepared,and the smallest deviation from the theoretical value was 1.4 *** translocation measurement results revealed that the prepared SCS nanopores possessed the excellent potential to be applied in biosensing.

关键词： smallest precise sizes

Correction of thermal airflow distortion in warpage measurements of microelectronic packaging structures via deep learning-based digital image correlation

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Microsystems & Nanoengineering 2024年第4期10卷 403-414页

作者： Yuhan Gao Yuxin Chen Ziniu Yu Chuanguo Xiong xin lei Weishan Lv Sheng Liu Fulong Zhu Institute of Microsystems School of Mechanical Science and EngineeringHuazhong University of Science and TechnologyWuhan 430074China School of Power and Mechanical Engineering Wuhan UniversityWuhan 430072China

The projected speckle-based three-dimensional digital image correlation method(3D-DIC)is being increasingly used in the reliability measurement of microelectronic packaging structures because of its noninvasive nature,high precision,and low ***,during the measurement of the thermal reliability of packaging structures,the thermal airflow generated by heating introduces distortions in the images captured by the DIC measurement system,impacting the accuracy and reliability of noncontact *** address this challenge,a thermal airflow distortion correction model based on the transformer attention mechanism is proposed specifically for the measurement of thermal warpage in microelectronic packaging *** model avoids the oversmoothing issue associated with convolutional neural networks and the lack of physical constraints in generative adversarial networks,ensuring the precision of grayscale gradient changes in speckle patterns and minimizing adverse effects on DIC calculation *** inputting the distorted images captured by the DIC measurement system into the network,corrected images are obtained for 3D-DIC calculations,thus allowing the thermal warpage measurement results of the sample to be *** experiments measuring topography with customized step block specimens,the effectiveness of the proposed method in improving warpage measurement accuracy is confirmed;this is particularly true when captured images are affected by thermal airflow at 140°C and 160°C,temperatures commonly encountered in thermal reliability testing of packaging *** method successfully reduces the standard deviation from 9.829 to 5.943μm and from 12.318 to 6.418μm,*** results demonstrate the substantial practical value of this method for measuring thermal warpage in microelectronic packaging structures.

关键词： thermal distortion image

ATP-induced supramolecular assembly based on chromophoric organic molecules and metal complexes

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Chinese Chemical Letters 2024年第11期35卷 87-98页

作者： Zhu Shu xin lei Yeye Ai Ke Shao Jianliang Shen Zhegang Huang Yongguang Li College of Material Chemistry and Chemical EngineeringKey Laboratory of Organosilicon Chemistry and Material Technology Ministry of EducationHangzhou Normal UniversityHangzhou 311121China National Engineering Research Center of Ophthalmology and Optometry Eye HospitalWenzhou Medical UniversityWenzhou 325027China Zhejiang Engineering Research Center for Tissue Repair Materials Wenzhou InstituteUniversity of Chinese Academy of SciencesWenzhou 325001China School of Chemistry Sun Yat-sen UniversityGuangzhou 510275China

Adenosine triphosphate(ATP),known as a common metabolic product in organism,is not only importance to provide energy in various cellular activities but also is widely explored in the bio-inspired synthetic supramolecular area which becomes a fascinating topic with the rapid development of biology,chemistry and materials *** this review,the recent advances about ATP interacted with functional small organic compounds and metal coordinated complexes are *** design principles,its function as an active supramolecular matrix,the associated non-covalent binding modes and assembly induced properties including the optical properties,morphologies are presented in ***,their applications for metal ion detecting,enzyme activity monitoring and drug delivery are described due to their excellently dynamic assembly properties,adjustability,and response to ***,an overview of the existing challenges and future prospects of ATP-induced supramolecular systems are also discussed.

关键词： Adenosine triphosphate Metal complexes Luminescence Assembly Monitoring application

Research advancement of DNA-based intelligent hydrogels:Manufacture, characteristics, application of disease diagnosis and treatment

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Chinese Chemical Letters 2024年第10期35卷 92-101页

作者： Zhe-Han Yang Jie Yin lei xin Yuanfang Li Yijie Huang Ruo Yuan Ying Zhuo Chongqing Key Laboratory of Catalysis and Functional Organic Molecules College of Environment and ResourcesChongqing Technology and Business UniversityChongqing 400067China Key Laboratory of Luminescence and Real-Time Analytic Chemistry(Southwest University) Ministry of EducationCollege of Chemistry and Chemical EngineeringSouthwest UniversityChongqing 400715China

DNA-based hydrogels are exceptional materials for biological applications because of their numerous advantages such as biodegradability,biocompatibility,hydrophilicity,super absorbency,porosity,and *** these advantages,the ability of DNA-based hydrogels to respond to specific physical and chemical triggers and undergo reversible phase transitions has garnered significant attention in the fields of disease diagnosis(biosensors)and treatment(drug delivery).This article focuses on the recent advancements in the research of DNA-based hydrogels and discusses the different types of these hydrogels,the synthetic methods,their unique properties,and their applications in biosensors and drug *** types of DNA hydrogels are categorized based on their building blocks,and the process of synthesis as well as the unique characteristics of DNA-based hydrogels are ***,DNA-based responsive hydrogels utilized as intelligent materials for the development of biosensors are ***,this article also presents the current status of DNA-based responsive hydrogels in drug delivery for cancer treatment,wound healing,and other therapeutic ***,this paper discusses the current challenges in expanding the practical application of DNA-based hydrogels.

关键词： DNA-based hydrogel Intelligent hydrogel Manufacture Characteristics Diseasediagnosis and treatment

Performance of a double-layer BAF using zeolite and ceramic as media under ammonium shock load condition

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Water Science and Engineering 2014年第1期7卷 81-89页

作者： xin-lei ZHAO Liang ZHU Shi-jie BAI Ming ZHOU Jing QIAN Wei WU College of Environment Hohai University Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes Ministry of EducationHohai University Yellow River Engineering Consulting Co. Ltd. State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering Hohai University Wujin Water Resources Bureau

An experiment was carried out to investigate the anti-ammonium shock load capacity of a biological aerated filter （BAF） composed of a double-layer bed. This bed was made up of a top layer of ceramic and a bottom layer of zeolite. The experiment shows that the anti-ammonium shock load process can be divided into two processes： adsorption and release. In the adsorption process, the total removal efficiency of ammonia nitrogen by zeolite and ceramic was 94%. In the release process, the ammonia nitrogen concentration increased significantly and then gradually returned to the normal level four hours after the shock load. The results indicated that the double-layer BAF had a high level of adaptability to the short-term ammonium shock load and long-term operation. The main factors influencing the dynamic process of ammonia nitrogen adsorption were the filter bed height, ammonia nitrogen concentration of influent, and flow rate. The bed depth service time （BDST） model was used to predict the relationship between the filter bed height and breakthrough time at different flow rates, and the results are reliable.

关键词： biological aerated filter (BAF) zeolite ceramic ammonia nitrogen shock load, dynamic adsorption

CHAIN:unlocking informatics-aided design of Li metal anode from materials to applications

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Rare Metals 2022年第5期41卷 1477-1489页

作者： Li-Sheng Zhang xin-lei Gao xin-Hua Liu Zheng-Jie Zhang Rui Cao Han-Chao Cheng Ming-Yue Wang Xiao-Yu Yan Shi-Chun Yang School of Transportation Science and Engineering Beihang UniversityBeijing 100191China

With the rapid development of consumer electronics,electric vehicles and grid-scale stationary energy storage,high-energy batteries are urgently demanded at *** metal batteries(LMBs)are considered to be one of the most promising high-energy density energy storage devices at present and have received much attention due to their ultra-high theoretical capacity,extremely low electrochemical potential and light ***,critical issues,such as uncontrollable lithium dendrite growth,dynamic changes in volume,interfacial impedance,severe chemical and electrochemical corrosion,remain huge challenges for Li metal anodes,which not only lead to low Columbic efficiency of LMBs,but also pose the risk of internal short circuit,causing serious side reactions and safety concerns that hinder LMBs from practical ***,lithium metal is gradually poised for a revival after decades of oblivion,due to the development of research tools and nanotechnologybased *** this review,various recent material designs for lithium metal anodes are reviewed based on previous theoretical understanding and *** Li dendrites and ensuring the long life span of practical batteries through limited Li metal anodes design are still ***-scale modeling methods are concerned,requiring the application of electrode material *** multi-scale modeling application methods with machine learning technology are proposed based on the cloud computing *** material designs for Li metal anodes on model information are integrated with artificial ***,this review provides a novel framework for next-generation lithium metal anode design methods with a digital solution based on multi-scale data-driven models and machine learning techniques.

关键词： Li metal anodes Energy materials Multi-scale modeling Fusion of model and data Digital twin CHAIN

Can the efficiencies of simplified perovskite solar cells go higher?

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Journal of Energy Chemistry 2021年第7期30卷 33-36页

作者： Jin-Feng Liao Wenhuai Feng Jun-xing Zhong Bing-xin lei Wu-Qiang Wu Key Laboratory of Bioinorganic and Synthetic Chemistry(MOE) School of ChemistrySun Yat-sen UniversityGuangzhou 510275GuangdongChina School of Chemistry and Chemical Engineering Key Laboratory of Electrochemical Energy Storage and Energy Conversion of Hainan ProvinceHainan Normal UniversityHaikou 571158HainanChina

In recent years, metal halide perovskites have emerged as star semiconducting materials in the field of optoelectronic devices owing to their fascinating optoelectronic properties. Of particular interest are perovskite solar cells (PSCs), which have witnessed skyrocketing power conversion efficiencies (PCEs) within a short period of time, and were recently certified to reach 25.5%, which is already higher than other thin film photovoltaic technologies[1]. Nevertheless, multiple layers are still needed for state-of-theart PSCs to achieve high PCEs over 21%.

关键词： Perovskite solar cells Charge extraction Charge recombination Interfacial modification Energy level alignment

Effect of Dy doping on magnetostrictive and mechanical properties of Fe83Ga17 alloy

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China Foundry 2020年第3期17卷 198-205页

作者： xin-lei Wang Yuan Liu Xiang Chen Hua-wei Zhang Yan-xiang Li School of Materials Science and Engineering Tsinghua UniversityBeijing 100084China Key Laboratory for Advanced Materials Processing Technology MOEChina

Fe83Ga17 alloy is a kind of promising magnetostrictive alloys with high magnetostrictive properties and a low saturation magnetic ***-cast Fe83Ga17 Dyx(x=0,0.05,0.1,0.2,0.4)polycrystalline alloys were prepared by arc *** of Dy doping on the microstructure,magnetostrictive and mechanical properties of as-cast Fe83Ga17 alloy was *** show that Dy-doped alloys exhibit a dual-phase structure containing the A2 matrix and Dy-rich precipitates(Fe56Ga34Dy10).Both magnetostriction and mechanical properties of Fe83Ga17 alloys are improved by Dy doping.A small amount of Dy addition(x=0.2)significantly causes Fe83Ga17 alloy to transform from typical brittle material(fracture strainε<1%)to plastic material(ε≈11%).Correspondingly,the fracture mode transforms from intergranular fracture to dimple *** the same time,the ultimate tensile strength and the magnetostriction rise up to 209 MPa and 64 ppm,***-rich precipitates disperse along the grain boundries and inside the grains,which plays an important role in the grain refinement and solution strengthening,and therefore,contribute to the enhancement of mechanical properties of the *** improvement of magnetostriction could be attributed to the large lattice distortion induced by Dy atoms entering into the A2 *** Dy into Fe-Ga alloys provides an effective solution to the brittleness in their applications.

关键词： Fe-Ga alloys Dy doping mechanical properties magnetostriction