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Visually Intracellular Detection of Telomerase Activity Based on DNA Strand Displacement Reaction and Gold Nanoparticle Labeling

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Chinese Journal of Chemistry 2022年第6期40卷 693-698页

作者： Yajun Zhang Shen Yan Zhaoxin Chen xin jiang Shuang Rao Zhuoran jiang Shanshan Qin Xiang Zhou Yuhao Du College of Chemistry and Molecular Sciences Key Laboratory of Biomedical Polymers of Ministry of EducationWuhan UniversityWuhanHubei 430072China

Telomerase,which is regarded as a common biomarker for early cancer diagnostics and a potential target for clinical therapies,has attracted considerable interests concerning its detection and ***,we propose a sensitive method by designing a gold nanoparticle(AuNP)probe for visually intracellular detection of telomerase *** AuNPs were functionalized with a telomer-ase substrate primer(SH-prime).A 6-carboxy-fluorescein(FAM)modified strand(FAM-probe)was attached to the surface of AuNP through its complementary stand(SH-attach).In the absence of telomerase,the fluorescence resonance energy transfer(FRET)from FAM to AuNPs results in efficient fluorescence *** the presence of telomerase,SH-primers on AuNPs were extended with the repeat units(TTAGGGG)*** extension sequence triggered the strand displacement of FAM-probe to restore the fluorescence *** is worth mentioning that the proposed strategy does not need to design complex hairpin structure and allows the meas-urement of telomerase in crude cell extracts down to 0.5 HeLa cells/μL in 2 *** addition,the present sensing platform can be ap-plied to the visually intracellular detection of telomerase activity in living cells.

关键词： Telomerase Nanoparticles DNA strand displacement Intracellular FRET

Research advances in genetic engineering of plantibody

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Chinese Science Bulletin 1999年第5期44卷 390-397页

作者： Junzhi Zeng Yu Fu Xiaocong Yu xin jiang Hualiang Huang Chinese Acad Sci Genet Inst Beijing 100101 Peoples R China

The combination of engineering antibody and plant biotechnology creates the plantibody. It has been reported that the engineering antibodies expressed in plant, no matter whether they are intact or small-molecular antibodies, keep their antigen-binding specificity, which means they are *** trait makes plantibody notable. Now the researches are focused mainly on the following three aspects: ( ⅰ ) Therapeutic antibody in clinic. It is expected to offer the opportunity of large-scale antibody production in agriculture systems, for the purpose of decreasing the cost greatly. Moreover, the property of multiple sexual hybridization between plants can be used to produce bi-or multi-functional antibodies and create new antibody medicines. (ⅱ) Plant physiology. Engineering antibodies against plant hormones or some active materials can block their activities in plant, so the trait of plantibody could be used to study the growth and development of plant. ( ⅲ ) Plant disease-resistance. The gene of

关键词： antibody gene transgenic plant gene expression plantibody.

Effects of biochars on the bioaccessibility of phenanthrene/pyrene/zinc/lead and microbial community structure in a soil under aerobic and anaerobic conditions

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Journal of Environmental Sciences 2018年第1期30卷 296-306页

作者： Ni Ni Renyong Shi Zongtang Liu Yongrong Bian Fang Wang Yang Song xin jiang Key Laboratory of Soil Environment and Pollution Remediation Institute of Soil ScienceChinese Academy of SciencesNanjing 210008China University of the Chinese Academy of Sciences Beijing 100049China jiangsu Key Laboratory for Bioresources of Saline Soils Yancheng Teachers UniversityYanchengJiangsu 224051China

The immobilization of co-contaminants of organic and inorganic pollutants by biochar is an efficient remediation strategy. However, the effect of biochar amendments on the bioaccessibility of the co-contaminants in dry versus flooded soils has rarely been compared. In batch experiments, bamboo-derived biochar（BB） had a higher sorption capacity for phenanthrene（Phe）/pyrene（Pyr）/zinc（Zn） than corn straw-derived biochar（CB）, while CB had a higher sorption capacity for lead（Pb） than BB. After 150 days of incubation, the amendments of 2% CB, 0.5% BB and 2% BB effectively suppressed the dissipation and reduced the bioaccessibility of Phe/Pyr by 15.65%/18.02%, 17.07%/18.31%and 25.43%/27.11%, respectively, in the aerobic soils. This effectiveness was more significant than that in the anaerobic soils. The accessible Zn/Pb concentrations were also significantly lower in the aerobic soils than in the anaerobic soils, regardless of *** Gram-negative bacterial biomass and the Shannon–Weaver index in the aerobic soil amended with 2% CB were the highest. The soil microbial community structure was jointly affected by changes in the bioaccessibility of the co-contaminants and the soil physiochemical properties caused by biochar amendments under the two conditions. Therefore, dry land farming may be more reliable than paddy soil cultivation at reducing the bioaccessibility of Phe/Pyr/Zn/Pb and enhancing the soil microbial diversity in the short term.

关键词： Soil utilization type Bioaccessibility Biochar Co-contaminants PLFA

Relationships between humic substance-bound mercury contents and soil properties in subtropical zone

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Journal of Environmental Sciences 2006年第5期18卷 951-957页

作者： YU Gui-fen WU Hong-tao jiang xin HE Wen-xiang QING Chang-le Institute-of Soil Science Chinese Academy of Sciences Nanjing 210008 China College of Environmental Sciences Southwest Agricultural University Chongqing 400716 China

The bioavailability of humic substance-bound mercury （HS-Hg） has been established, while the distribution of HS-Hg in soils in relation to soil properties remains obscure. Path analysis and principal component analysis were employed in present study to investigate how soil factors influence the contents of HS-Hg in soils. Results showed that HS-Hg ranged from 0.0192 to 0.2051 mg/kg in soils. The two fractions existed in soils as humic acid-bound mercury （HA-Hg）〉 fulvic acid-bound mercury （FA-Hg） and the ratio of HA-Hg/FA-Hg was 1.61 on the average. Soil organic carbon （OC） and HS favorably determined soil HS-Hg and the two fractions. The mercury source forming HS-Hg derived from soil total mercury and HS-Hg. FA-Hg and HA-Hg served as mercury source for each other. In acidic soils, FA-Hg and HA-Hg consistently rose with the increase of OC, and generally HA-Hg increased more dramatically. Soils with lower pH and lighter texture contained more HS-Hg, particularly fraction of FA-Hg. Among all influencing factors, organic material source showed the strongest effect, followed by other soil properties and soil mercury source.

关键词： humic acid-bound Hg fulvic acid-bound Hg soil properties

Oligopeptide Self-Assembly:Mechanisms,Stimuli-Responsiveness,and Biomedical Applications

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CCS Chemistry 2024年第1期6卷 69-90页

作者： Lei Ge Hanyan Xu xin jiang Jing Yu School of Materials Science and Engineering Nanyang Technological UniversitySingapore 637553 Institute for Digital Molecular Analytics and Science Nanyang Technological UniversitySingapore 637553

Oligopeptide self-assembly materials have emerged as a promising class of biomaterials with diverse applications in *** review highlights the recent progress in comprehending the selfassembly mechanisms intrinsic to oligopeptides and their behavior in response to specific *** methodically structuring the amino acid sequence and managing external stimuli such as pH levels,redox conditions,or enzymatic activity,we can exercise unprecedented control over the self-assembly *** controlling the self-assembly process of oligopeptides,various structures with extraordinary versatility can be obtained,including micelles,nanofibers,and coacervate droplets,each possessing modifiable mechanical and chemical ***,these self-assembled constructs demonstrate immense potential within varied biomedical *** stimuli-sensitive nature of oligopeptide assembly materials facilitates timely encapsulation and release of therapeutic cargos,consequently eliciting desired cellular *** approach paves the way for more precise tumor targeting,personalized medicinal treatments,and well-regulated drug *** innate biocompatibility and proficiency in replicating the extracellular matrix(ECM)render them ideally suited for applications such as tissue engineering,wound remediation,and regenerative *** summary,oligopeptide self-assembling materials show tremendous potential as adaptable platforms for cutting-edge biomedical applications,thereby bridging the divide between fundamental research and practical clinical application.

关键词： oligopeptides self-assembly nonequilibrium stimuli-responsive behavior biomedical applications

Behavior and control of chlorine in dyestuff residue incineration

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Journal of Environmental Sciences 2006年第3期18卷 577-582页

作者： YAN Jian-hua TAN Zhong-xin jiang Xue-guang CHI Yong CEN Ke-fa State key Laboratory of Clean Energy Institute for Thermal Power Engineering Zhejiang University Hangzhou 310027 China

Dyestuffresidue, a type of hazardous waste, is incinerated in the tubular furnace, and thermodynamic equilibrium model is used to calculate and analyze the chlorine behavior. The HCI emission and its effects on the behaviors of heavy metals are studied. Meanwhile, the effects of three dechlorine reagents are predicted at a high temperature. Results show that HCI emission is dependent on incineration temperature. The HCl evaporated mainly derives from the organic chlorine. Under the working condition of 500-- 900℃, the main products of rig, Pb, Cu, Ni, Zn, and Mn in reaction with HCl are HgCl2 （g）, PbCl4（g）, PbCI2 （g）, （CuCl）3 （g）, NiCl2 （s）, NiCl2 （g）, ZnCl2 （s）, ZnCl2 （g）, Zn （g）, MnCl2 （s）, and MnCl2 （g）, respectively. Among the three dechlorine reagents, CaCO3 is optimal to remove chlorine at high temperature, little of HCl is released below 800℃, whereas Fe3O4 is unstable at high temperature.

关键词： hazardous waste thermodynamic equilibrium model HCl heavy metals dechlorine reagents

On-Chip Micro Temperature Controllers Based on Freestanding Thermoelectric Nano Films for Low-Power Electronics

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Nano-Micro Letters 2024年第7期16卷 98-108页

作者： Qun Jin Tianxiao Guo Nicolas Perez Nianjun Yang xin jiang Kornelius Nielsch Heiko Reith Institute for Metallic Materials Leibniz Institute for Solid State and Materials Research01069 DresdenGermany Institute of Materials Engineering University of Siegen57076 SiegenGermany Institute of Applied Physics Technical University of Dresden01069 DresdenGermany Institute of Materials Science Technical University of Dresden01069 DresdenGermany

Multidimensional integration and multifunctional com-ponent assembly have been greatly explored in recent years to extend Moore’s Law of modern ***,this inevitably exac-erbates the inhomogeneity of temperature distribution in microsystems,making precise temperature control for electronic components extremely ***,we report an on-chip micro temperature controller including a pair of thermoelectric legs with a total area of 50×50μm^(2),which are fabricated from dense and flat freestanding Bi2Te3-based ther-moelectric nano films deposited on a newly developed nano graphene oxide membrane *** tunable equivalent thermal resistance is controlled by electrical currents to achieve energy-efficient temperature control for low-power electronics.A large cooling temperature difference of 44.5 K at 380 K is achieved with a power consumption of only 445μW,resulting in an ultrahigh temperature control capability over 100 K mW^(-1).Moreover,an ultra-fast cooling rate exceeding 2000 K s^(-1) and excellent reliability of up to 1 million cycles are *** proposed on-chip temperature controller is expected to enable further miniaturization and multifunctional integration on a single chip for microelectronics.

关键词： Temperature control Low-power electronics On-chip micro temperature controller Freestanding thermoelectric nano films Temperature-sensitive components

Direct Nitrous Oxide Emissions Related to Fertilizer-Nitrogen, Precipitation, and Soil Clay Fraction: Empirical Models

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Atmospheric and Oceanic Science Letters 2015年第5期8卷 277-282页

作者： ZHANG Wei GU jiang-xin ZHENG Xun-Hua State Key Laboratory of Atmospheric Boundary Layer Physics and Atmospheric Chemistry (LAPC) Institute of Atmospheric Physics Chinese Academy of Sciences University of Chinese Academy of Sciences College of Natural Resources and Environment Northwest A & F University

Direct nitrous oxide(N2O) emissions(DNEs) from croplands are required in national inventories of greenhouse gases. The Intergovernmental Panel on Climate Change(IPCC) guidelines provide an approach using direct emission factors(EFds) to estimate DNEs, which are constants for large regions. The goal of this paper is to establish empirical models to account for the temporal and spatial variations of EFds, which, apart from the nitrogen addition rate, also vary with a range of environmental factors, so as to enhance the accuracy of regional/national DNE estimates. Therefore, the seasonal/annual DNEs(n = 71) from upland croplands, which are the differences in N2 O emissions between fields with and without fertilizer-nitrogen addition, were used to statistically relate DNEs to regulating factors including the fertilizer-nitrogen addition rate(FN), and environmental(climate and soil) factors. The multivariate stepwise linear regression results showed positive combined effects of FN and clay fraction on DNEs(R2 = 0.61, p < 0.001). Furthermore, the nonlinear regression of FN, precipitation, and clay fraction was also adopted for prediction(R2 = 0.50, p < 0.001). Validation with an independent dataset(n = 31) suggested that both models were better predictors of DNEs than the IPCC model, which only depends on FN. These empirical models may provide simple but reliable approaches for compiling regional/national, and even global inventories of DNEs from croplands. However, both models were restricted to a limited sample size. Understandably, more field observations are still required to further validate the global applicability of these simple approaches.

关键词： nitrous oxide direct emissions empirical model nit

Effect of slag on oxide inclusions in carburized bearing steel during industrial electroslag remelting

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International Journal of Minerals,Metallurgy and Materials 2019年第3期26卷 291-300页

作者： Shi-jian Li Guo-guang Cheng Zhi-qi Miao Lie Chen xin-yan jiang State Key Laboratory of Advanced Metallurgy University of Science and Technology Beijing xining Special Steel Group Co. Ltd.

Industrial experiments with three types of slags were performed to investigate the effect of slag on oxide inclusions during electroslag remelting(ESR) process. G20CrNi2Mo bearing steel was used as the consumable electrode and remelted using a 2400-kg industrial furnace. The results showed that most inclusions in the electrode were low-melting-point CaO-MgO-Al_2O_3. After ESR, all the inclusions in ingots were located outside the liquid region. When the slag consisted of 65.70 wt% CaF_2, 28.58 wt% Al_2O_3, and 4.42 wt% CaO was used, pure Al_2O_3 were the dominant inclusions in ingot, some of which presented a clear trend of agglomeration. When the ingot was remelted by a multi-component slag with 16.83 wt% CaO, a certain amount of sphere CaAl_4O_7 inclusions larger than 5 μm were generated in ingot. The slag with 8.18 wt% CaO exhibited greater capacity to control the inclusion characteristics. Thermodynamic calculations indicated that the total Ca and Mg in ingots were attributed from the relics in electrode and strongly influenced by the slag composition. The formation of ingot inclusions was calculated by FactSage^(TM) 7.0, and the results were basically in accordance with the observed inclusions, indicating that a quasi-thermodynamic equilibrium could be obtained in the metal pool.

关键词： bearing steel electroslag remelting slag inclusions thermodynamics