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Biomaterials and tissue engineering in traumatic brain injury:novel perspectives on promoting neural regeneration

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Neural Regeneration Research 2024年第10期19卷 2157-2174页

作者： Shihong Zhu xiaoyin Liu Xiyue lu Qiang Liao Huiyang luo Yuan Tian Xu Cheng Yaxin Jiang Guangdi Liu Jing Chen Department of Neurosurgery West China HospitalWest China Medical SchoolSichuan UniversityChengduSichuan ProvinceChina National Engineering Research Center for Biomaterials College of Biomedical EngineeringSichuan UniversityChengduSichuan ProvinceChina Department of Anesthesiology West China HospitalWest China Medical SchoolSichuan UniversityChengduSichuan ProvinceChina Department of Pharmacy West China HospitalWest China Medical SchoolSichuan UniversityChengduSichuan ProvinceChina Out-patient Department West China HospitalWest China Medical SchoolSichuan UniversityChengduSichuan ProvinceChina Department of Respiratory and Critical Care Medicine West China HospitalWest China Medical SchoolSichuan UniversityChengduSichuan ProvinceChina

Traumatic brain injury is a serious medical condition that can be attributed to falls, motor vehicle accidents, sports injuries and acts of violence, causing a series of neural injuries and neuropsychiatric symptoms. However, limited accessibility to the injury sites, complicated histological and anatomical structure, intricate cellular and extracellular milieu, lack of regenerative capacity in the native cells, vast variety of damage routes, and the insufficient time available for treatment have restricted the widespread application of several therapeutic methods in cases of central nervous system injury. Tissue engineering and regenerative medicine have emerged as innovative approaches in the field of nerve regeneration. By combining biomaterials, stem cells, and growth factors, these approaches have provided a platform for developing effective treatments for neural injuries, which can offer the potential to restore neural function, improve patient outcomes, and reduce the need for drugs and invasive surgical procedures. Biomaterials have shown advantages in promoting neural development, inhibiting glial scar formation, and providing a suitable biomimetic neural microenvironment, which makes their application promising in the field of neural regeneration. For instance, bioactive scaffolds loaded with stem cells can provide a biocompatible and biodegradable milieu. Furthermore, stem cells-derived exosomes combine the advantages of stem cells, avoid the risk of immune rejection, cooperate with biomaterials to enhance their biological functions, and exert stable functions, thereby inducing angiogenesis and neural regeneration in patients with traumatic brain injury and promoting the recovery of brain function. Unfortunately, biomaterials have shown positive effects in the laboratory, but when similar materials are used in clinical studies of human central nervous system regeneration, their efficacy is unsatisfactory. Here, we review the characteristics and properties

关键词： bioactive materials biomaterials exosomes neural regeneration scaffolds stem cells tissue engineering traumatic brain injury

Tubulin detyrosination promotes human trophoblast syncytium formation

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Journal of Molecular Cell Biology 2019年第11期11卷 967-978页

作者： Rui Wang Ruoxuan Yu Cheng Zhu Hai-Yan Lin xiaoyin lu Hongmei Wang State Key Laboratory of Stem Cell and Reproductive Biology Institute of ZoologyChinese Academy of SciencesBeijingChina Department of Experimental Radiation Oncology the University of Texas MD Anderson Cancer CenterHoustonTXUSA University of Chinese Academy of Sciences BeijingChina

Human trophoblast syncytialization is one of the most important yet least understood events during placental *** this study,we found that detyrosinated a-tubulin(detyr-a-tub),which is negatively regulated by tubulin tyrosine Ugase(TTL),was elevated during human placental cytotrophoblast ***,relatively high expression of TTL protein was observed in first-trimester human placental cytotrophoblast cells,but fusing trophoblast cells exhibited much lower levels of T T L Notably,fusion of preeclamptic cytotrophoblast cells was compromised but could be partially rescued by knockdown of TTL ***,chronic downregulation of TTL in trophoblast cells resulted in significantly elevated expression of *** of detyr-a-tub thus contributed to the cell surface localization of the fusogenic protein Syncytin-2 and the gap junction protein Connexin 43(Cx43),which in turn promoted successful fusion between trophoblast *** together,the results suggest that tubulin detyrosination plays an essential role in human trophoblast fusogenic protein aggregation and *** tubulin detyrosination leads to defects in syncytialization and potentially to the onset of preeclampsia.

关键词： TTL detyrosination syncytialization preeclampsia placenta syncytin

Variations in mesenchymal-epithelial transition-related transcription factors during reprogramming of somatic cells from different germ layers into iPSCs

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Journal of Genetics and Genomics 2016年第10期43卷 609-612页

作者： xiaoyin lu Yukai Wang Long Yan Libin Wang Wei Li Hongmei Wang State Key Laboratory of Stem Cell and Reproductive Biology Institute of Zoology Chinese Academy of Sciences University of Chinese Academy of Sciences

Introducing a combination of transcription factors such as Oct4,Sox2,Klf4 and c-Myc（OSKM）enables reprogramming which converts somatic cells into induced pluripotent stem cells（i PSCs）（Takahashi and Yamanaka,2006）.i PSCs play an important role in clinical and regenerative medicine because they can be utilized to model a specific disease or differentiate into functional cells for *** the efficiency of induction and improving the qualities of iPSCs are constant themes in this field.

关键词： PSCs Variations in mesenchymal-epithelial transition-related transcription factors during reprogramming of somatic cells from different germ layers into iPSCs

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高速摄影仪成像噪声去除方法研究

仪器仪表学报 2023年第2期44卷 211-220页

作者：陈怀安卢小银单奕萌阚艳金一中国科学技术大学工程科学学院合肥230026 合肥中科君达视界技术股份有限公司合肥230051 国营芜湖机械厂芜湖241000

高速摄影仪在超高帧率下(>10000 FPS)易产生噪声,该噪声分布复杂,难以获取与有噪图像完全对应的清晰图像。针对该问题,提出一种基于非理想配对图像的卷积去噪网络训练方法。首先利用高速和低速摄影仪拍摄相同场景图像,获得有噪图像及与... 详细信息

高速摄影仪在超高帧率下(>10000 FPS)易产生噪声,该噪声分布复杂,难以获取与有噪图像完全对应的清晰图像。针对该问题,提出一种基于非理想配对图像的卷积去噪网络训练方法。首先利用高速和低速摄影仪拍摄相同场景图像,获得有噪图像及与其对应的非理想配对清晰图像;然后,建立基于卷积神经网络的深度去噪模型,结合亮度一致化和图像对齐方法,实现非理想配对图像的监督学习,从而去除成像噪声;最后,引入模型量化技术将模型参数和激活值由32位浮点数量化为8位定点数,降低模型大小、内存需求和运行时间。实验结果表明,提出的去噪方法可有效去除高速摄影仪成像噪声,相比于其他方法,去噪图像峰值信噪比提高1.96 dB,结构相似性提高1.95%;通过模型量化,模型大小降低4倍,内存需求降低45.62%,运行时间降低37.5%。

关键词：高速摄影图像去噪非理想配对卷积神经网络模型量化

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不同品种水稻发芽阶段耐盐性对比研究

中国农学通报 2022年第33期38卷 10-14页

作者：伊嘉雯冯棣朱崴亓娜滕奉魁卢小引潍坊科技学院山东寿光262700 天津天隆科技股份有限公司天津300457 国家粳稻工程技术研究中心天津300457

为了对比不同品种水稻发芽阶段的耐盐性,筛选出耐盐性更强的品种,以0、0.3%、0.5%NaCl处理水稻种子,分析不同程度盐胁迫下9个水稻品种生长指标的差异。结果表明,在水稻发芽阶段,0.3%和0.5%NaCl处理下显著抑制其出芽和生长,其中‘9311’... 详细信息

为了对比不同品种水稻发芽阶段的耐盐性,筛选出耐盐性更强的品种,以0、0.3%、0.5%NaCl处理水稻种子,分析不同程度盐胁迫下9个水稻品种生长指标的差异。结果表明,在水稻发芽阶段,0.3%和0.5%NaCl处理下显著抑制其出芽和生长,其中‘9311’水稻品种变化最小。与无盐处理相比,仅在0.5%NaCl处理下的芽长和根长显著降低,降幅分别为11.9%和11.5%。盐分胁迫对水稻种子发芽率的抑制程度高低顺序为‘G1’>‘银香38’>‘津原U99’>‘软香粳’>‘金稻919’>‘津原77’>‘津原E28’>‘天隆粳2号’>‘9311’。综合分析结果显示,随着盐浓度的升高,水稻的生长发育受到明显抑制,在9个水稻品种中‘9311’的耐盐性最强。

关键词：水稻品种盐分胁迫发芽率耐盐性

子痫前期上调lncRNA MALAT1对缺氧诱导的滋养细胞功能损伤的作用机制探究

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中国计划生育和妇产科 2024年第6期16卷 71-76,82页

作者：卢智健李晓茵卢倩瑜陈志萍叶旭彬东莞市人民医院妇产科广东东莞523000

目的探究子痫前期(PE)患者胎盘组织中长链非编码RNA MALAT1(lncRNA MALAT1)的表达,及其对缺氧诱导的滋养细胞功能损伤的作用。方法收集2020年5月至2022年10月东莞市人民医院20例PE患者(PE组)和20例健康对照(NP组)的胎盘组织,采用定量... 详细信息

目的探究子痫前期(PE)患者胎盘组织中长链非编码RNA MALAT1(lncRNA MALAT1)的表达,及其对缺氧诱导的滋养细胞功能损伤的作用。方法收集2020年5月至2022年10月东莞市人民医院20例PE患者(PE组)和20例健康对照(NP组)的胎盘组织,采用定量逆转录聚合酶链式反应(qRT-PCR)检测MALAT1和自噬相关基因LC3和Beclin-1 mRNA在两组胎盘组织中的表达差异。体外培养人滋养层细胞系HTR-8,采用瞬时转染技术将过表达MALAT1质粒(MALAT1)或其阴性对照(pc-NC)质粒转染入细胞中。低氧(Hyp)条件处理上述细胞,并将其分为4组:Control组,Hyp+pc-NC组,Hyp+MALAT1组和自噬抑制剂3-甲基腺嘌呤(3-MA)处理的Hyp+MALAT1+3-MA组。CCK-8法检测各组细胞的增殖活力,Transwell法检测各组细胞的侵袭能力,TUNEL染色法检测各组细胞的凋亡,Western blotting实验检测各组细胞中凋亡相关蛋白Bax、Bcl-2、cleved caspase-3的表达和自噬相关蛋白Beclin-1蛋白表达和LC3-Ⅱ/Ⅰ比值。结果与NP组相比,MALAT1、LC3和Beclin-1 mRNA在PE组中的表达均显著降低(P<0.01)。与Control组相比,Hyp+pc-NC组、Hyp+MALAT1组和Hyp+MALAT1+3-MA组的细胞增殖活力、侵袭能力、Bcl-2与Beclin-1蛋白表达和LC3-Ⅱ/Ⅰ比值均显著降低(P<0.05),但细胞凋亡率、Bax和cleved caspase-3蛋白的表达均显著升高(P<0.05)。与Hyp+pc-NC组相比,Hyp+MALAT1组细胞增殖活力、侵袭能力、Bcl-2与Beclin-1蛋白表达和LC3-Ⅱ/Ⅰ比值均明显升高(P<0.05),而细胞凋亡率、Bax和cleved caspase-3蛋白的表达均显著降低(P<0.05);Hyp+MALAT1+3-MA组细胞增殖活力、侵袭能力、Bcl-2与Beclin-1蛋白表达和LC3-Ⅱ/Ⅰ比值均显著降低(P<0.05),但细胞凋亡率、Bax和cleved caspase-3蛋白的表达水平均显著升高(P<0.05)。与Hyp+MALAT1组相比,Hyp+MALAT1+3-MA组细胞增殖活力、侵袭能力、Bcl-2与Beclin-1蛋白表达和LC3-Ⅱ/Ⅰ比值均显著降低(P<0.05),细胞凋亡率、Bax和cleved caspase-3蛋白的表达水平均显著升高(P<0.05)。结论 MALAT1在PE患者胎盘组织中的表达显著降低,上调其表达可通过促进细胞自噬水平改善低氧诱导的滋养细胞增殖和侵袭能力损伤,并抑制细胞的凋亡。

关键词：子痫前期长链非编码RNA MALAT1 低氧自噬滋养细胞

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牛肉宰后成熟过程中细胞自噬的变化分析

食品科学 2024年第15期45卷 214-221页

作者：李叶鲁文洁王海蓉杨啸吟刘昀阁梁荣蓉毛衍伟张一敏朱立贤山东农业大学食品科学与工程学院国家牛肉加工技术研发专业中心山东泰安271018

本研究分析宰后牛肉成熟14 d过程中细胞自噬的变化规律及其与牛肉品质间的关系。牛肉宰后成熟过程中,肉色方面,L*值随成熟时间延长而升高,a*值、b*值均呈现先升高后降低的趋势;嫩度随成熟时间延长不断提高,剪切力与肌间线蛋白(Desmin)... 详细信息

本研究分析宰后牛肉成熟14 d过程中细胞自噬的变化规律及其与牛肉品质间的关系。牛肉宰后成熟过程中,肉色方面,L*值随成熟时间延长而升高,a*值、b*值均呈现先升高后降低的趋势;嫩度随成熟时间延长不断提高,剪切力与肌间线蛋白(Desmin)表达量均呈现下降趋势(P<0.05);保水性随成熟时间的延长而下降,贮藏损失率和蒸煮损失率上升(P<0.05)。进一步对自噬相关蛋白进行研究,牛肉宰后成熟过程中,Beclin-1与微管相关蛋白1轻链3作为细胞自噬发生的标志性蛋白,其表达量均呈先升高后降低的趋势,并在12 h达到最大。调控自噬的AMP活化蛋白激酶(AMP-activated protein kinase,AMPK)-哺乳动物雷帕霉素靶蛋白(mammalian target of rapamycin,mTOR)-酵母Atg1同源蛋白激酶(UNC-51 like kinase 1,ULK1)通路作为一条重要的能量代谢通路,在缺血缺氧环境下能够诱导细胞自噬的发生。本研究结果表明,牛肉宰后成熟过程中AMPK、p-AMPKThr172、ULK1的蛋白表达量均呈先升高后降低的趋势,并在12 h达到最大,mTOR表达量则持续下降(P<0.05),说明细胞自噬在宰后0~12 h增强。相关性分析结果显示,宰后自噬相关蛋白表达水平与牛肉的肉色呈负相关,与蒸煮损失、剪切力、Desmin蛋白相对表达量呈正相关(P<0.05)。综上所述,宰后牛肉成熟336h过程中可能通过AMPK-mTOR-ULK1通路激活细胞自噬进而对牛肉品质起到一定的调控作用。

关键词：牛肉宰后成熟细胞自噬品质

Broadband high-efficiency polymerized liquid crystal metasurfaces with spin-multiplexed functionalities in the visible

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Photonics Research 2022年第6期10卷 1380-1393页

作者： XINJIAN lu xiaoyin LI YINGHUI GUO MINGBO PU JIANGYU WANG YAXIN ZHANG XIONG LI XIAOLIANG MA XIANGANG luO State Key Laboratory of Optical Technologies on Nano-Fabrication and Micro-Engineering Institute of Optics and ElectronicsChinese Academy of SciencesChengdu 610209China School of Optoelectronics University of Chinese Academy of SciencesBeijing 100049China Vector Light Field Research Center Institute of Optics and ElectronicsChinese Academy of SciencesChengdu 610209China School of Optoelectronic Science and Engineering University of Electronic Science and Technology of ChinaChengdu 610054China

Traditional optical components are usually designed for a single functionality and narrow operation band,leading to the limited practical *** date,it is still quite challenging to efficiently achieve multifunctional performances within broadband operating bandwidth via a single planar optical ***,a broadband high-efficiency polarization-multiplexing method based on a geometric phase polymerized liquid crystal metasurface is proposed to yield the polarization-switchable functionalities in the *** proofs of the concept,two broadband high-efficiency polymerized liquid crystal metalenses are designed to obtain the spin-controlled behavior from diffraction-limited focusing to sub-diffraction focusing or focusing vortex *** experimental results within a broadband range indicate the stable and excellent optical performance of the planar liquid crystal *** addition,low-cost polymerized liquid crystal metasurfaces possess unique superiority in large-scale patterning due to the straightforward processing technique rather than the point-by-point nanopatterning method with high cost and low *** high-efficiency liquid crystal metasurfaces also have unrivalled advantages benefiting from the characteristic with low waveguide *** proposed strategy paves the way toward multifunctional and high-integrity optical systems,showing great potential in mobile devices,optical imaging,robotics,chiral materials,and optical interconnections.

关键词： polarization interconnection waveguide

IGF2BP3-induced activation of EIF5B contributes to progression of hepatocellular carcinoma cells

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Oncology Research 2022年第2期30卷 77-87页

作者： xiaoyin LI QIAN WANG HONGFENG LIANG SHISHENG CHEN HAIWEN CHEN YAOYONG lu CHANGFU YANG Department of Oncology(Section 3) Gaozhou People’s HospitalGaozhouChina South China Research Center for Acupuncture and Moxibustion Medical College of Acu-Moxi and RehabilitationGuangzhou University of Chinese MedicineGuangzhouChina The Second Affiliated Hospital of Guangzhou University of Chinese Medicine(Guangdong Provincial Hospital of Chinese Medicine) GuangzhouChina Affiliated Nanfang Hospital(Branch Tai He Hospital)of Southern Medical University GuangzhouChina

In this study,we investigated the functional role of eukaryotic initiation factor 5B(EIF5B)in hepatocellular carcinoma(HCC)and the underlying *** analysis demonstrated that the EIF5B transcript and protein levels as well as the EIF5Bcopy number were signiﬁcantly higher in the HCC tissues compared with the non-cancerous liver ***-regulation of EIF5B signiﬁcantly decreased proliferation and invasiveness of the HCC ***,EIF5B knockdown suppressed epithelial-mesenchymal transition(EMT)and the cancer stem cell(CSC)***-regulation of EIF5B also increased the sensitivity of HCC cells to 5-ﬂuorouracil(5-FU).In the HCC cells,activation of the NF-kappa B signaling pathway and IkB phosphorylation was signiﬁcantly reduced by EIF5B ***2BP3 increased the stability of the EIF5B mRNA in an m6A-dependent *** data suggested that EIF5B is a promising prognostic biomarker and therapeutic target in HCC.

关键词： EIF5B NF-jB Epithelial-mesenchymal transition Cancer stem cells N6-methyladenosine Hepatocellular carcinoma