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贵州荞麦重要种质的遗传多样性分析及分子身份证构建

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华中农业大学学报 2024年第3期43卷 148-157页

作者：冷宇鑫闵义付阳云周奎何颖文晓鹏贵州大学生命科学学院/农业生物工程研究院/山地植物资源保护与种质创新教育部重点实验室贵阳550025

为精准鉴定贵州荞麦种质资源,采用SSR分子标记对60份荞麦种质进行遗传多样性分析,构建DNA分子身份证数据库。结果显示,从100对SSR引物中筛选出16对稳定性好、多态性丰富的引物,在60份供试种质中共扩增出174个多态性条带;Shannon’s信息... 详细信息

为精准鉴定贵州荞麦种质资源,采用SSR分子标记对60份荞麦种质进行遗传多样性分析,构建DNA分子身份证数据库。结果显示,从100对SSR引物中筛选出16对稳定性好、多态性丰富的引物,在60份供试种质中共扩增出174个多态性条带;Shannon’s信息指数、Nei’s多样性指数、多态信息指数均值分别为0.337、0.206、0.693,引物的多态性较好,能有效揭示60份荞麦种质的遗传多样性;在Dice遗传相似系数为0.374时,所有供试材料可聚为A、B、C三组;当Dice遗传相似系数为0.484时,将苦荞组(A组)更细分为A1、A2两个小组。采用毛细管电泳及8%的聚丙烯酰胺凝胶(PAGE)电泳对SSR标记扩增产物进行双验证,2种方法的聚类结果一致。结果表明,本研究开发的高效性SSR分子标记能够有效地鉴定贵州荞麦重要种质的遗传多样性且用于构建分子身份证。

关键词：荞麦 SSR标记毛细管电泳遗传多样性 DNA分子身份证

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贵州百香果主要种植区病毒种类的检测

热带作物学报 2024年第7期45卷 1478-1488页

作者：闫艺心乔光周奎张曼莹李蕊蕊文晓鹏贵州大学生命科学学院/农业生物工程研究院/山地植物资源保护与种质创新教育部重点实验室贵州贵阳550025

百香果(Passiflora edulis)作为贵州特色优势水果,在巩固脱贫攻坚成果和深入实施乡村振兴战略中具有重要意义。但近年来百香果病毒病危害日趋严重,造成产量和品质急剧下降,严重影响产业发展。为确定贵州百香果主要种植区病毒种类、分布... 详细信息

百香果(Passiflora edulis)作为贵州特色优势水果,在巩固脱贫攻坚成果和深入实施乡村振兴战略中具有重要意义。但近年来百香果病毒病危害日趋严重,造成产量和品质急剧下降,严重影响产业发展。为确定贵州百香果主要种植区病毒种类、分布及复合侵染情况,本研究从贵州省罗甸县、从江县、榕江县、镇宁县和贞丰县5个百香果主要种植区采集疑似感病叶片153份,采用RT-PCR技术对其进行检测。结果表明:样本共检测到7种病毒,分别是东亚西番莲病毒(East Asian Passiflora virus,EAPV)、夜来香花叶病毒(Telosma mosaic virus,TeMV)、黄瓜花叶病毒(Cucumber mosaic virus,CMV)、芜菁花叶病毒(Turnip mosaic virus,TuMV)、西番莲斑驳病毒(Passiflora mottle virus,PaMV)、西番莲潜隐病毒(Passiflora latene carlavirus,PLV)和马铃薯Y病毒(Potato virus Y,PVY)。EAPV检出率最高,达96.08%,以下依次为TeMV(81.70%)、CMV(78.43%)、TuMV(44.44%)、PaMV(26.80%)、PVY(24.84%)和PLV(15.03%);值得重视的是,PVY主要为害马铃薯,而侵染百香果为首次报道。本次检测到的7种病毒的测序结果与相应参考序列的核酸一致性均在95%以上,经构建进化树发现,EAPV、TeMV、PaMV、PLV、TuMV分别与中国各省份的百香果病毒分离物存在一定的地理聚集性,而CMV和PVY分别与西班牙CMV甜瓜分离物和卢旺达PVY辣椒分离物亲缘关系最近,这说明这些病毒来源及感染途径复杂,未在国内大面积传播。本次共检测到24种复合侵染类型,以3~5种复合侵染类型为主,复合侵染率达94.77%,其中EAPV+TeMV+CMV组合是复合侵染中出现最多的类型;EAPV、TeMV和CMV的检出率高达78.43%~96.08%,在5个百香果主要种植区均有检出,且在多种复合侵染类型中普遍存在,因此这3种病毒为侵染贵州百香果主要种植区的优势病毒。此外,本研究建立了EAPV、TeMV和CMV的多重PCR检测体系,为相关病毒的快速检测提供技术支持。

关键词：贵州百香果病毒检测 RT-PCR 复合侵染

光照对仙人掌科植物蓬莱宫中磷酸钙晶体形貌的影响

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生物学杂志 2024年第4期41卷 83-87页

作者：李昌颖秦利鸿郑登月杜倩陈春丽文晓鹏贵州大学、生命科学学院、农业生物工程研究院、山地植物资源保护与保护种质创新教育部重点实验室、山地生态与农业生物工程协同创新中心贵阳550025 华中农业大学生命科学技术学院武汉430070

研究发现仙人掌科植物——蓬莱宫中存在花束状的晶体,在此基础上对蓬莱宫疣部进行扫描电镜观察和能谱分析,结果表明,该矿物晶体含O、Ca、P元素,该类矿物质为磷酸钙晶体,主要镶嵌于皮层细胞壁上,极少分布在细胞中,其形貌大多呈四周散开... 详细信息

研究发现仙人掌科植物——蓬莱宫中存在花束状的晶体,在此基础上对蓬莱宫疣部进行扫描电镜观察和能谱分析,结果表明,该矿物晶体含O、Ca、P元素,该类矿物质为磷酸钙晶体,主要镶嵌于皮层细胞壁上,极少分布在细胞中,其形貌大多呈四周散开或向一个方向展开的花束状,偶然观察到有膜包裹的橄榄球状;对蓬莱宫弱光处理后观察发现,表型明显发生变化,颜色从深绿变为嫩绿,茎伸长,疣间距变宽,变细长,刺较软向四周微张;疣中晶体密度显著减少,且磷酸钙晶片小部分聚集在一起,晶体尺寸也显著缩小(P<0.001)。研究结果为对植物中磷酸钙的形貌特征及其功能的理解提供了参考。

关键词：生物矿化蓬莱宫磷酸钙形貌特征扫描电镜

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Effects of Manganese on the Antioxidant System and Related Gene Expression Levels in the “Hong Yang” Kiwifruit Seedlings

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Phyton-International Journal of Experimental Botany 2023年第8期92卷 2399-2412页

作者： Chongpei Zheng Liangliang Li Zhencheng Han Weijie Li xiaopeng wen Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region(Ministry of Education) Institute of Agro-BioengineeringCollege of Life SciencesGuizhou UniversityGuiyang550025China Institute of Mountain Resource Guizhou Academy of SciencesGuiyang550001China

To explore how manganese affects the antioxidant system and the expression levels of related genes of“Hong yang”seedlings,the leaves of its tissue cultured seedlings were taken as test materials,and single factor treatment was performed by changing the manganese chloride(MnCl_(2)·4H_(2)O)solution concentration when spraying the *** expression levels of Mn-SOD,POD64 and POD27 genes in leaves were quantitatively analyzed by real-time quantitative PCR(qRT-PCR)at different determination ***,the contents of malondial-dehyde(MDA),hydrogen peroxide(H_(2)O_(2)),the activities of antioxidant enzymes,including catalase(CAT),peroxidase(POD),and superoxide dismutase(SOD).The results showed that the SOD,CAT,POD,ascorbate peroxidase(APX),and reduced glutathione(GSH)activities in leaves were the highest at 12 h post-treatment with 50μM MnCl_(2)·4H_(2)***,the contents of MDA and H_(2)O_(2) in leaves also peaked when the concentration of H_(2)O_(2) is 50μM,which is the minimum *** at 50μM Mn^(2+),the Mn-SOD and POD27 expression was up-regulated as compared to the control,which promoted the expression of their respective enzyme ***,POD64 expression increased with the increasing Mn^(2+) ***,50μM is the optimal concentration of Mn when exogenously applied on“Hong yang”,which improve the antioxidant enzyme activity and regulate the plant’s physiological and biochemical functions.

关键词： “Hong yang”seedlings manganese antioxidant system related gene expression

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填精益髓汤加减治疗脑梗死后痴呆的疗效观察

辽宁中医杂志 2024年第7期51卷 100-103页

作者：刘亚鸽李坤彬姚先丽吴志远温小鹏李小杏郑州大学附属郑州市中心医院神经康复科河南郑州450000

目的探讨填精益髓汤加减治疗脑梗死后痴呆的疗效。方法选取2020年4月—2022年6月于该院进行治疗的128例脑梗死后痴呆患者,依据随机数字表法分为对照组与观察组,各64例。两组均予以常规西医治疗,对照组采取填精益髓汤加减治疗,两组均治疗... 详细信息

目的探讨填精益髓汤加减治疗脑梗死后痴呆的疗效。方法选取2020年4月—2022年6月于该院进行治疗的128例脑梗死后痴呆患者,依据随机数字表法分为对照组与观察组,各64例。两组均予以常规西医治疗,对照组采取填精益髓汤加减治疗,两组均治疗8周。分析两组治疗疗效;比较两组治疗前后中医证候得分、认知功能[简易智力状态检查量表(MMSE)、长谷川痴呆量表(HDT)、画钟试验(CDT)]及炎症因子[白细胞介素-8(IL-8)、肿瘤坏死因子(TNF-α)、白细胞介素-6(IL-6)]。结果治疗前,两组中医证候得分、MMSE、HDS、CDT、IL-8、TNF-α、IL-6差异比较不具有统计学意义(P>0.05)。治疗后,观察组临床疗效、MMSE、HDS、CDT得分高于对照组,中医证候得分及IL-8、TNF-α、IL-6水平低于对照组,差异具有统计学意义(P<0.05)。结论脑梗死痴呆后患者采取填精益髓汤加减治疗临床疗效较好,可明显改善患者临床症状,促进认知功能恢复,降低炎症反应,可推广应用。

关键词：填精益髓汤脑梗死中医治疗临床疗效认知功能

补阳还五汤加减联合脑-肢协同调控治疗卒中后肢体功能障碍临床研究

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新中医 2024年第7期56卷 36-41页

作者：姚先丽李坤彬刘亚鸽温小鹏吴志远焦冰郑州大学附属郑州中心医院神经康复科河南郑州450006

目的:观察补阳还五汤加减联合脑-肢协同调控治疗脑卒中后肢体功能障碍的疗效。方法:选取96例卒中后肢体功能障碍患者为研究对象,按随机数字表法分为对照组及观察组各48例。对照组采取常规治疗加脑-肢协同调控,观察组在对照组基础上采用... 详细信息

目的:观察补阳还五汤加减联合脑-肢协同调控治疗脑卒中后肢体功能障碍的疗效。方法:选取96例卒中后肢体功能障碍患者为研究对象,按随机数字表法分为对照组及观察组各48例。对照组采取常规治疗加脑-肢协同调控,观察组在对照组基础上采用补阳还五汤加减治疗。比较2组临床疗效,比较2组治疗前后中医证候积分、血清指标[C-反应蛋白(CRP)、同型半胱氨酸(Hcy)]、血流变指标[纤维蛋白原(Fib)、血浆黏度、红细胞比容(HCT)]、Fugl-Meyer运动功能评分量表(FMA)、Berg平衡量表(BBS)、功能性步行量表(FAC)评分的变化。结果:治疗4周后,观察组临床疗效总有效率为95.83%,对照组为83.33%,2组比较,差异有统计学意义(P<0.05)。治疗后,2组中医证候各积分均较治疗前下降(P<0.05),观察组中医证候各积分均低于对照组(P<0.05)。治疗后,2组CRP、Hcy指标值均较治疗前下降(P<0.05),观察组上述2项指标值均低于对照组(P<0.05)。治疗4周后,2组Fib、血浆黏度、HCT指标值均较治疗前下降(P<0.05),观察组上述3项指标值均低于对照组(P<0.05)。治疗4周后,2组FMA、BBS、FAC评分均较治疗前升高(P<0.05),观察组评分均高于对照组(P<0.05)。结论:补阳还五汤加减联合脑-肢协同调控治疗卒中后肢体功能障碍能提升临床疗效,改善肌力、肌张力下降等症状,减轻炎症,促进肢体运动能力的恢复。

关键词：脑卒中肢体功能障碍补阳还五汤脑-肢协同调控 Fugl-Meyer运动功能评分量表

Transcriptome-wide identification and expression profiling of Pinus massoniana MYB transcription factors responding to phosphorus deficiency

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Journal of Forestry Research 2020年第3期31卷 909-919页

作者： Fuhua Fan Qingzhu Wang xiaopeng wen Guijie Ding Institute for Forest Resources and Environment of Guizhou Guizhou UniversityGuiyang 550025China College of Forestry Guizhou UniversityGuiyang 550025China Institute of Agro-Bioengineering Guizhou UniversityGuiyang 550025China

Myeloblastosis(MYB) proteins constitute one of the largest transcription factor(TF) families in plants and play crucial roles in regulating plant physiological and biochemical processes, including adaptation to diverse abiotic stresses. These TF families contain highly conserved MYB repeats(1 R, R2 R3, 3 R and 4 R) at the N-terminus. Roles for MYB TFs have been reported in response to such stresses as dehydration, salt, cold, and drought. The characterization of Masson pine(Pinus massoniana) MYB TFs are reported, including the analysis of MYB TFs expression in seedlings under controlled conditions and two different phosphate(Pi) deficient treatments. By searching for conserved MYB motifs in full transcriptomic RNA sequencing data for P. massoniana, 59 sequences were identified as MYB TFs. Conserved domainstructures and comparative functional and phylogenetic relationships of these MYB TFs with those in Arabidopsis were assessed using various bioinformatics tools. Based on microarray analysis, P. massoniana MYB genes exhibited different expression patterns under the two Pi deficiency conditions. Genes encoding MYB TFs that showed increased expression under critical Pi deficiency were identified, and some MYBs were differentially expressed only under conditions of severe Pi starvation. These results are useful for the functional characterization of MYB TFs that may be involved in the response to Pi deficiency and play divergent roles in plants.

关键词： MYB transcription factors Transcriptome Pinus massoniana Phosphorus deficiency Microarray analysis

Overview of epigenetic degraders based on PROTAC, molecular glue, and hydrophobic tagging technologies

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Acta Pharmaceutica Sinica B 2024年第2期14卷 533-578页

作者： xiaopeng Peng Zhihao Hu Limei Zeng Meizhu Zhang Congcong Xu Benyan Lu Chengpeng Tao Weiming Chen wen Hou Kui Cheng Huichang Bi Wanyi Pan Jianjun Chen College of Pharmacy Key Laboratory of Prevention and Treatment of Cardiovascular and Cerebrovascular Diseases of Ministry of EducationGannan Medical UniversityGanzhou 314000China College of Basic Medicine Gannan Medical UniversityGanzhou 314000China Guangdong Provincial Key Laboratory of New Drug Screening NMPA Key Laboratory for Research and Evaluation of Drug MetabolismSchool of Pharmaceutical SciencesSouthern Medical UniversityGuangzhou 510515China

Epigenetic pathways play a critical role in the initiation, progression, and metastasis of cancer. Over the past few decades, significant progress has been made in the development of targeted epigenetic modulators(e.g., inhibitors). However, epigenetic inhibitors have faced multiple challenges,including limited clinical efficacy, toxicities, lack of subtype selectivity, and drug resistance. As a result,the design of new epigenetic modulators(e.g., degraders) such as PROTACs, molecular glue, and hydrophobic tagging(Hy T) degraders has garnered significant attention from both academia and pharmaceutical industry, and numerous epigenetic degraders have been discovered in the past decade. In this review,we aim to provide an in-depth illustration of new degrading strategies(2017-2023) targeting epigenetic proteins for cancer therapy, focusing on the rational design, pharmacodynamics, pharmacokinetics, clinical status, and crystal structure information of these degraders. Importantly, we also provide deep insights into the potential challenges and corresponding remedies of this approach to drug design and development. Overall, we hope this review will offer a better mechanistic understanding and serve as a useful guide for the development of emerging epigenetic-targeting degraders.

关键词： Epigenetic Degrader PROTAC Molecularglue Hydrophobic tagging

Thermodynamic theory of flotation for a complex multiphase solid -liquid system and high-entropy flotation

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International Journal of Minerals,Metallurgy and Materials 2024年第6期31卷 1177-1197页

作者： Shuming wen State Key Laboratory of Complex Nonferrous Metal Resources Clean Utilization Faculty of Land Resource EngineeringKunming University of Science and TechnologyKunming 650093China

The flotation of complex solid–liquid multiphase systems involve interactions among multiple components,the core problem facing flotation ***,the combined use of multicomponent flotation reagents to improve mineral flotation has become an important issue in studies on the efficient use of refractory mineral ***,studying the flotation of complex solid–liquid systems is extremely difficult,and no systematic theory has been developed to *** addition,the physical mechanism associated with combining reagents to improve the flotation effect has not been unified,which limits the development of flotation theory and the progress of flotation *** this study,we applied theoretical thermodynamics to a solid–liquid flotation system and used changes in the entropy and Gibbs free energy of the reagents adsorbed on the mineral surface to establish thermodynamic equilibrium equations that de-scribe interactions among various material components while also introducing adsorption equilibrium constants for the flotation reagents adsorbed on the mineral *** homogenization effect on the mineral surface in pulp solution was determined using the chemical potentials of the material components of the various mineral surfaces required to maintain *** flotation effect can be improved through synergy among multicomponent flotation reagents;its physical essence is the thermodynamic law that as the number of compon-ents of flotation reagents on the mineral surface increases,the surface adsorption entropy change increases,and the Gibbs free energy change of adsorption *** to the results obtained using flotation thermodynamics theory,we established high-entropy flotation theory and a technical method in which increasing the types of flotation reagents adsorbed on the mineral surface,increasing the adsorption entropy change of the flotation reagents,decreasing the Gibbs free energy change,and improving the adsorption efficiency

关键词： multicomponent flotation reagent adsorption entropy change equations of thermodynamic equilibrium adsorption equilibri-um constant high-entropy flotation