检索结果-南通市图书馆

不同海拔毛竹叶绿素的高光谱特征及叶绿素估测模型

在线全文

学校读者我要写书评

暂无评论

东北林业大学学报 2024年第9期52卷 46-52,57页

作者：孟繁钰郭孝玉郑小曼福建农林大学福州350002 三明学院

叶绿素是表征植被生产力的重要指标,毛竹叶绿素快速检测对农林作物高效精准经营具有重要意义。以福建永安上坪乡山地竹林为对研究对象,设置3个海拔梯度(低海拔、中海拔、高海拔)采集不同冠层毛竹叶片样本65份;每份样品重复测定3次高光... 详细信息

叶绿素是表征植被生产力的重要指标,毛竹叶绿素快速检测对农林作物高效精准经营具有重要意义。以福建永安上坪乡山地竹林为对研究对象,设置3个海拔梯度(低海拔、中海拔、高海拔)采集不同冠层毛竹叶片样本65份;每份样品重复测定3次高光谱和叶绿素质量分数,分析叶绿素质量分数与光谱特征随海拔梯度变化的关系,筛选对叶绿素质量分数变化影响敏感的光谱特征及光谱指标;应用5种参数回归方程和3种机器学习算法拟合毛竹叶绿素质量分数估测模型。结果表明:海拔是影响毛竹叶片光谱特征的重要因素,叶绿素质量分数随着海拔梯度上升呈现显著增加趋势,“三边”(蓝边、黄边和红边)参数与叶绿素质量分数的敏感程度不佳,原始光谱和一阶微分光谱与叶绿素质量分数在可见光范围内有多波段相关性显著;原始光谱曲线中,叶绿素敏感波长为683、890 nm,一阶微分光谱曲线中,叶绿素敏感波长为749 nm。以红边波段叶绿素指数(I_(CIred))建立的二次函数回归模型的R^(2)为0.613;随机森林模型(RF)的R2达0.852,与参数模型精度相比精度提升38.9%,均方根误差下降61.0%,平均相对误差下降38.6%;I_(CIred)+RF组合模型为毛竹叶片叶绿素质量分数的单变量最佳估算模型。

关键词：高光谱海拔梯度毛竹叶绿素光谱指数机器学习模型

Tumor microenvironment of cancer stem cells:Perspectives on cancer stem cell targeting

在线全文

学校读者我要写书评

暂无评论

Genes & Diseases 2024年第3期11卷 275-302页

作者： Qianqian Guo Yi Zhou Tianyuan Xie Yin Yuan Huilong Li Wanjin Shi Lufeng zheng xiaoman Li Wenzhou Zhang Department of Pharmacy The Affiliated Cancer Hospital of Zhengzhou University&Henan Cancer HospitalZhengzhouHenan 450003China Jiangsu Key Laboratory for Pharmacology and Safety Evaluation of Chinese Materia Medica School of PharmacyNanjing University of Chinese MedicineNanjingJiangsu 210023China School of Life Science and Technology Jiangsu Key Laboratory of Carcinogenesis and InterventionChina Pharmaceutical UniversityNanjingJiangsu 211198China

There are few tumor cell subpopulations with stem cell characteristics in tumor tis-sue,defined as cancer stem cells(CSCs)or cancer stem-like cells(CSLCs),which can recon-struct neoplasms with malignant biological behaviors such as invasiveness via self-renewal and unlimited *** microenvironment that CsCs depend on consists of various cellular components and corresponding medium *** these factors existing at a variety of levels and forms,cytokine networks and numerous signal pathways play an important role in signaling *** factors promote or maintain cancer cell stem-ness,and participate in cancer recurrence,metastasis,and *** review aims to summarize the recent molecular data concerning the multilayered relationship between CsCs and CsC-favorable *** also discuss the therapeutic implications of target-ing this synergistic interplay,hoping to give an insight into targeting cancer cell stemness for tumortherapyandprognosis.

关键词： Cancer stem cells Cancerstem-like cells Cellular factors Non-cellularfactors Tumor microenvironment

Modification of alternative splicing in bovine somatic cell nuclear transfer embryos using engineered CRISPR-Cas13d

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2022年第11期65卷 2257-2268页

作者： Rui Cheng xiaoman zheng Yingmei Wang Xing Ma Xin Liu Wenjun Xu Mengyun Wang Yuanpeng Gao Xupeng Xing Chuan Zhou Hongzheng Sun Zekun Guo Fusheng Quan Jun Liu Song Hua Yongsheng Wang Yong Zhang Xu Liu College of Veterinary Medicine Northwest A&F UniversityKey Laboratory of Animal Biotechnology of the Ministry of AgricultureYangling 712100China

Animal cloning can be achieved by somatic cell nuclear transfer(SCNT),but the resulting live birth rate is relatively *** previously improved the efficiency of bovine SCNT by exogenous melatonin treatment or by overexpression of lysine-specific demethylase 4D(KDM4D)and 4E(KDM4E).In this study,we revealed abundant alternative splicing(AS)transitions during fertilization and embryonic genome activation,and demonstrated abnormal AS in bovine SCNT embryos compared with in vitro fertilized *** used the CRISPR-Cas13d RNA-targeting system to target cis-elements of ABI2 and ZNF106 pre-m RNA to modify AS,thus reducing the ratio of abnormal-isoform SCNT embryos by nearly 50%and achieving a high survival rate(11%–19%).These results indicate that this system may provide an efficient method for bovine cloning,while also paving the way for further improvements in the efficiency of SCNT.

关键词： animal cloning alternative splicing CRISPR-Cas13d bovine

Uncovering key salt-tolerant regulators through a combined eQTL and GWAS analysis using the super pan-genome in rice

在线全文

学校读者我要写书评

暂无评论

National Science Review 2024年第4期11卷 55-69页

作者： Hua Wei Xianmeng Wang Zhipeng Zhang Longbo Yang Qianqian Zhang Yilin Li Huiying He Dandan Chen Bin Zhang Chongke zheng Yue Leng Xinglan Cao Yan Cui Chuanlin Shi Yifan Liu Yang Lv Jie Ma Wenchuang He Xiangpei Liu Qiang Xu Qiaoling Yuan xiaoman Yu Tianyi Wang Hongge Qian Xiaoxia Li Bintao Zhang Hong Zhang Wu Chen Mingliang Guo Xiaofan Dai Yuexing Wang Xiaoming zheng Longbiao Guo Xianzhi Xie Qian Qian Lianguang Shang Shenzhen Branch Guangdong Laboratory of Lingnan Modern AgricultureGenome Analysis Laboratory of the Ministry of Agriculture and Rural AffairsAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural Sciences Institute of Wetland Agriculture and Ecology Shandong Academy of Agricultural Sciences State Key Laboratory of Rice Biology China National Rice Research Institute National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop Science Chinese Academy of Agricultural Sciences Yazhouwan National Laboratory

For sessile plants, gene expression plays a pivotal role in responding to salinity stress by activating or suppressing specific genes. However, our knowledge of genetic variations governing gene expression in response to salt stress remains limited in natural germplasm. Through transcriptome analysis of the Global Mini-Core Rice Collection consisting of a panel of 202 accessions, we identified 22 345 and 27 610expression quantitative trait loci associated with the expression of 7787 and 9361 e Genes under normal and salt-stress conditions, respectively, leveraging the super pan-genome map. Notably, combined with genome-wide association studies, we swiftly pinpointed the potential candidate gene STG5—a major salt-tolerant locus known as q STS5. Intriguingly, STG5 is required for maintaining Na+/K+homeostasis by directly regulating the transcription of multiple members of the Os HKT gene family. Our study sheds light on how genetic variants influence the dynamic changes in gene expression responding to salinity stress and provides a valuable resource for the mining of salt-tolerant genes in the future.

关键词： super pan-genome expression quantitative trait loci GWAS salt tolerance rice

Ⅳ期非小细胞肺癌临床特征与生存预后的相关性分析

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

中国热带医学 2024年第5期24卷 571-578页

作者：盛佳丽张慧慧毕小慢郑少江海南医学院第一附属医院肿瘤研究所重大疾病生物样本资源海南省工程研究中心海南海口570102

目的通过探讨Ⅳ期非小细胞肺癌(non-small cell lung cancer,NSCLC)的临床特征与生存预后情况,为NSCLC预后评价及防治提供参考依据。方法以海南医学院第一附属医院肿瘤内科、呼吸内科2016—2020年收治的经病理学确诊且可供分析研究的19... 详细信息

目的通过探讨Ⅳ期非小细胞肺癌(non-small cell lung cancer,NSCLC)的临床特征与生存预后情况,为NSCLC预后评价及防治提供参考依据。方法以海南医学院第一附属医院肿瘤内科、呼吸内科2016—2020年收治的经病理学确诊且可供分析研究的195例Ⅳ期NSCLC患者为研究对象进行回顾性分析。收集患者的住院病历资料及随访资料,统计随访截止时患者的生存情况。采用Kaplan-Meier法计算生存率,利用Log-rank法进行分层变量对生存情况的影响进行单因素分析,通过多因素Cox回归模型分析患者生存预后的危险因素。结果Ⅳ期非小细胞肺癌患者中位生存时间为17.05(95%CI:12.64~21.45)个月,1年、2年和3年累积生存率分别是70.7%、41.5%和22.0%。多因素分析结果提示,性别(HR=0.697,95%CI:0.486~0.999,P=0.049),功能状态评分标准(Karnofsky,KPS)(HR=1.535,95%CI:1.038~2.270,P=0.032),计算机断层扫描(computed tomography,CT)肿瘤位置(HR=1.481,95%CI:1.003~2.186,P=0.036),病理类型(HR=1.181,95%CI:0.715~1.950,P=0.019),转移部位(HR=1.710,95%CI:1.214~2.409,P=0.002),N分期(HR=2.094,95%CI:0.973~4.509,P=0.006),基因突变(HR=2.387,95%CI:1.590~3.584,P<0.001),含化疗方案治疗(HR=1.713,95%CI:1.094~2.683,P=0.019),综合治疗(HR=1.874,95%CI:1.253~2.802,P=0.002)是影响Ⅳ期非小细胞肺癌患者生存预后的独立影响因素(均P<0.05)。亚组分析中,转移部位、含化疗治疗方案是影响Ⅳ期NSCLC基因突变阳性患者生存的独立预后影响因素,接受靶向治疗的患者生存期较长。Ⅳ期NSCLC基因突变阴性或未知患者中,转移部位、含化疗治疗方案、综合治疗是影响Ⅳ期NSCLC患者生存预后影响因素。结论本研究中性别、KPS评分、CT肿瘤位置、病理类型、转移部位、N分期、基因突变、含化疗方案治疗、综合治疗是影响Ⅳ期非小细胞肺癌患者生存预后的重要因素;在治疗方案上,化疗仍是不可或缺的基本治疗方案,另外,综合治疗相比较单一治疗方案能够延长生存期,存在基因突变阳性、服用靶向药物的患者生存时间较长。因此,在Ⅳ期NSCLC的治疗中应检测基因突变状态,选择对应的靶向药物延长生存期。

关键词：非小细胞肺癌 Ⅳ期临床特征预后因素

A centromere map based on super pan-genome highlights the structure and function of rice centromeres

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2024年第2期66卷 196-207页

作者： Yang Lv Congcong Liu Xiaoxia Li Yueying Wang Huiying He Wenchuang He Wu Chen Longbo Yang Xiaofan Dai Xinglan Cao xiaoman Yu Jiajia Liu Bin Zhang Hua Wei Hong Zhang Hongge Qian Chuanlin Shi Yue Leng Xiangpei Liu Mingliang Guo Xianmeng Wang Zhipeng Zhang Tianyi Wang Bintao Zhang Qiang Xu Yan Cui Qianqian Zhang Qiaoling Yuan Noushin Jahan Jie Ma Xiaoming zheng Yongfeng Zhou Qian Qian Longbiao Guo Lianguang Shang State Key Laboratory of Rice Biology China National Rice Research InstituteHangzhou 310006China Shenzhen Branch Guangdong Laboratory of Lingnan Modern AgricultureGenome Analysis Laboratory of the Ministry of Agriculture and Rural AffairsAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518120China Yazhouwan National Laboratory No.8 Huanjin RoadYazhou DistrictSanya City 572024China National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop ScienceChinese Academy of Agricultural SciencesBeijing 100081China

Rice(Oryza sativa)is a significant crop worldwide with a genome shaped by various evolutionary *** centromeres are crucial for chromosome segregation,and contain some unreported *** to the diverse and complex centromere region,a comprehensive understanding of rice centromere structure and function at the population level is *** constructed a high-quality centromere map based on the rice super pangenome consisting of a 251-accession panel comprising both cultivated and wild species of Asian and African *** showed that rice centromeres have diverse satellite repeat CentO,which vary across chromosomes and subpopulations,reflecting their distinct evolutionary *** also revealed that long terminal repeats(LTRs),especially young Gypsy-type LTRs,are abundant in the peripheral CentO-enriched regions and drive rice centromere expansion and ***,high-quality genome assembly and complete telomere-to-telomere(T2T)reference genome enable us to obtain more centromeric genome information despite mapping and cloning of centromere genes being *** investigated the association between structural variations and gene expression in the rice centromere.A centromere gene,OsMAB,which positively regulates rice tiller number,was further confirmed by expression quantitative trait loci,haplotype analysis and clustered regularly interspaced palindromic repeats(CRISPR)/CRISPR-associated protein9 *** revealing the new insights into the evolutionary patterns and biological roles of rice centromeres,our finding will facilitate future research on centromere biology and crop improvement.

关键词： centromere super pan-genome CentoO satellite repeat rice

磁性固相萃取-气质联用-同位素内标法精准检测白酒中8种高级脂肪酸乙酯

在线全文

学校读者我要写书评

暂无评论

食品科学技术学报 2024年第3期42卷 58-69页

作者：陈晓漫连旭东叶思廷安明哲赵东郑佳孙啸涛董蔚北京工商大学酿酒分子工程中国轻工业重点实验室/北京市食品风味化学重点实验室北京100048 五粮液集团有限公司四川宜宾511500

高级脂肪酸乙酯是引起酒体浑浊的关键因素之一。为系统分析白酒中的高级脂肪酸乙酯以改善白酒生产过程中的浑浊问题,建立了一种基于氨基化四氧化三铁(Fe_(3)O_(4)-NH_(2))磁性固相萃取结合气质联用技术的方法,用以同时测定白酒中8种高... 详细信息

高级脂肪酸乙酯是引起酒体浑浊的关键因素之一。为系统分析白酒中的高级脂肪酸乙酯以改善白酒生产过程中的浑浊问题,建立了一种基于氨基化四氧化三铁(Fe_(3)O_(4)-NH_(2))磁性固相萃取结合气质联用技术的方法,用以同时测定白酒中8种高级脂肪酸乙酯。通过考察酒样的pH值、离子强度、吸附剂用量、吸附时间、解吸溶剂种类、解吸溶剂酸碱度、解吸时间等因素,确定了较佳的萃取条件。结果表明:这8种高级脂肪酸乙酯在各自的质量浓度范围内线性关系良好,相关系数R 2均大于0.99;方法检出限和定量限分别为9.1~42.9μg/L和5.2~200.0μg/L;回收率为79.6%~99.3%,相对标准偏差(n=3)为8.0%~10.7%。应用此方法,并结合同位素内标法对24种不同香型和品牌的白酒样品中8种高级脂肪酸乙酯进行精准定量,发现它们的种类和质量浓度存在显著差异。其中,在酱香型白酒中,高级脂肪酸乙酯的检出率最高,达到100%。该方法简便快捷,样品萃取只需10 min,具有高灵敏度和准确性,能够有效避免传统液-液萃取技术的背景干扰问题,可为实现白酒中高级脂肪酸乙酯的精准检测提供一种新的方法。

关键词：高级脂肪酸乙酯浑浊磁性固相萃取同位素内标白酒

同方期刊数据库

OsPRMT6a-mediated arginine methylation of OsJAZ1 regulates jasmonate signaling and spikelet development in rice

在线全文

学校读者我要写书评

暂无评论

Molecular Plant 2024年第6期17卷 900-919页

作者： Kun Dong Fuqing Wu Siqi Cheng Shuai Li Feng Zhang Xinxin Xing Xin Jin Sheng Luo Miao Feng Rong Miao Yanqi Chang Shuang Zhang xiaoman You Peiran Wang Xin Zhang Cailin Lei Yulong Ren Shanshan Zhu Xiuping Guo Chuanyin Wu Dong-Lei Yang Qibing Lin Zhijun Cheng Jianmin Wan State Key Laboratory of Crop Gene Resources and Breeding National Key Facility for Crop Gene Resources and Genetic ImprovementInstitute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China State Key Laboratory for Crop Genetics and Germplasm Enhancement Nanjing Agricultural UniversityNanjing 210095China

Although both protein arginine methylation(PRMT)and jasmonate(JA)signaling are crucial for regulating plant development,the relationship between these processes in the control of spikelet development remains *** this study,we used the CRISPR/Cas9 technology to generate two OsPRMT6a loss-of-function mutants that exhibit various abnormal spikelet ***,we found that OsPRMT6a can methylate arginine residues in JA signal repressors OsJAZ1 and *** showed that arginine methylation of OsJAZ1 enhances the binding affinity of OsJAZ1 with the JA receptors OsCOI1a and OsCOI1b in the presence of JAs,thereby promoting the ubiquitination of OsJAZ1 by the SCF^(OsCOI1a/OsCOI1b) complex and degradation via the 26S *** process ultimately releases OsMYC2,a core transcriptional regulator in the JA signaling pathway,to activate or repress JA-responsive genes,thereby maintaining normal plant(spikelet)***,in the osprmt6a-1 mutant,reduced arginine methylation of OsJAZ1 impaires the interaction between OsJAZ1 and OsCOI1a/OsCOI1b in the presence of *** a result,OsJAZ1 proteins become more stable,repressing JA responses,thus causing the formation of abnormal spikelet ***,we discovered that JA signaling reduces the OsPRMT6a mRNA level in an OsMYC2-dependent manner,thereby establishing a negative feedback loop to balance JA *** further found that OsPRMT6a-mediated arginine methylation of OsJAZ1 likely serves as a switch to tune JA signaling to maintain normal spikelet development under harsh environmental conditions such as high ***,our study establishes a direct molecular link between arginine methylation and JA signaling in rice.

关键词： protein arginine methylation jasmonate signaling negative feedback loop spikelet development adaptation to heat stress rice

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

施主掺杂提高ZnO压敏电阻的冲击稳定性

陶瓷学报 2024年第2期45卷 352-359页

作者：杨莉禹任鑫宁宇刘晓曼高丽游俊玮姚政上海大学理学院纳米科学与技术研究中心上海200444

研究了铝铟共掺杂对ZnO压敏电阻的微观结构和电学性能的影响,特别是浪涌冲击稳定行为。采用X射线衍射、扫描电子显微镜对ZnO压敏电阻的微观结构进行了表征,通过电流—电压测试、脉冲电流冲击测试和电容—电压测试对电性能进行了评估。... 详细信息

研究了铝铟共掺杂对ZnO压敏电阻的微观结构和电学性能的影响,特别是浪涌冲击稳定行为。采用X射线衍射、扫描电子显微镜对ZnO压敏电阻的微观结构进行了表征,通过电流—电压测试、脉冲电流冲击测试和电容—电压测试对电性能进行了评估。结果表明:当Al^(3+)和In^(3+)掺杂量分别为0.0008 mol%和0.0033 mol%时,ZnO压敏电阻表现出了最佳的电性能;此时的电位梯度为95.3 V·mm^(-1)、非线性系数为74、残压比为2.43。另外,ZnO压敏电阻在8/20μs 10 k A电流下冲击20次后,正反压敏电压变化率分别为+3.30%和-6.67%。

关键词： ZnO压敏电阻施主掺杂电性能浪涌冲击稳定性