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Molecular Plant 2020年第3期13卷 414-430页

作者： Lijuan Qi Shan Liu Cong Li Jingying Fu Yanjun Jing Jinkui Cheng Hong Li Dun Zhang xiaoji wang xiaojing Dong Run Han Bosheng Li Yu Zhang Zhen Li William Terzaghi Chun-Peng Song Rongcheng Lin Zhizhong Gong Jigang Li State Key Laboratory of Plant Physiology and Biochemistry College of Biological SciencesChina Agricultural UniversityBeijing 100193China Key Laboratory of Photobiology Institute of BotanyChinese Academy of SciencesBeijing 100093China Institute of Plant and Food Science Department of BiologySouthern University of Science and TechnologyShenzhenGuangdong 518055China Department of Plant and Microbial Biology University of CaliforniaBerkeleyCA 94720USA Plant Gene Expression Center Agriculture Research ServiceU.S.Department of AgricultureAlbanyCA 94710USA Department of Biology Wilkes UniversityWilkes-BarrePA 18766USA Institute of Plant Stress Biology Collaborative Innovation Center of Crop Stress BiologyHenan UniversityKaifeng 475001China

PHYTOCHROME-INTERACTING FACTORS(PIFs)are a group of basic helix-loop-helix transcription factors that can physically interact with photoreceptors,including phytochromes and *** was previously demonstrated that PIFs accumulated in darkness and repressed seedling photomorphogenesis,and that PIFs linked different photosensory and hormonal pathways to control plant growth and *** this study,we show that PIFs positively regulate the ABA signaling pathway during the seedling stage specifically in *** found that PIFs positively regulate ABI5 transcript and protein levels in darkness in response to exogenous ABA treatment by binding directly to the G-box motifs in the ABI5 ***,PIFs and the G-box motifs in the ABI5 promoter determine ABI5 expression in darkness,and overexpression of ABI5 could rescue the ABA-insensitive phenotypes of pifq mutants in the ***,we discovered that PIFs can physically interact with the ABA receptors PYL8 and PYL9,and that this interaction is not regulated by *** analyses showed that PYL8 and PYL9 promote PIF4 protein accumulation in the dark and enhance PIF4 binding to the ABI5 promoter,but negatively regulate PIF4-mediated ABI5 *** together,our data demonstrate that PIFs interact with ABA receptors to orchestrate ABA signaling in darkness by controlling ABI5 expression,providing new insights into the pivotal roles of PIFs as signal integrators in regulating plant growth and development.

关键词： Arabidopsis transcription factor PIFs ABA ABI5 darkness

De novo assembly of a Tibetan genome and identification of novel structural variants associated with high-altitude adaptation

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National Science Review 2020年第2期7卷 391-402页

作者： Ouzhuluobu Yaoxi He Haiyi Lou Chaoying Cui Lian Deng Yang Gao wangshan Zheng Yongbo Guo xiaoji wang Zhilin Ning Jun Li Bin Li Caijuan Bai Baimakangzhuo Gonggalanzi Dejiquzong Bianba Duojizhuoma Shiming Liu Tianyi Wu Shuhua Xu Xuebin Qi Bing Su High Altitude Medical Research Center School of Medicine Tibetan University Fukang Obstetrics Gynecology and Children Branch Hospital Tibetan Fukang Hospital State Key Laboratory of Genetic Resources and Evolution Kunming Institute of Zoology Chinese Academy of Sciences Kunming College of Life Science University of Chinese Academy of Sciences Chinese Academy of Sciences Key Laboratory of Computational Biology CAS-MPG Partner Institute for Computational Biology Shanghai Institute of Nutrition and Health Shanghai Institutes for Biological SciencesUniversity of Chinese Academy of Sciences School of Life Science and Technology Shanghai Tech University Center for Disease Control Tibet Autonomous Region National Key Laboratory of High Altitude Medicine High Altitude Medical Research Institute Center for Excellence in Animal Evolution and Genetics Chinese Academy of Sciences Collaborative Innovation Center of Genetics and Development

Structural variants(SVs) may play important roles in human adaptation to extreme environments such as high altitude but have been under-investigated. Here, combining long-read sequencing with multiple scaffolding techniques, we assembled a high-quality Tibetan genome(ZF1), with a contig N50 length of 24.57 mega-base pairs(Mb) and a scaffold N50 length of 58.80 Mb. The ZF1 assembly filled 80 remaining N-gaps(0.25 Mb in total length) in the reference human genome(GRCh38). Markedly, we detected17 900 SVs, among which the ZF1-specific SVs are enriched in GTPase activity that is required for activation of the hypoxic pathway. Further population analysis uncovered a 163-bp intronic deletion in the MKL1 gene showing large divergence between highland Tibetans and lowland Han Chinese. This deletion is significantly associated with lower systolic pulmonary arterial pressure, one of the key adaptive physiological traits in Tibetans. Moreover, with the use of the high-quality de novo assembly, we observed a much higher rate of genome-wide archaic hominid(Altai Neanderthal and Denisovan) shared non-reference sequences in ZF1(1.32%–1.53%) compared to other East Asian genomes(0.70%–0.98%),reflecting a unique genomic composition of Tibetans. One such archaic hominid shared sequence—a662-bp intronic insertion in the SCUBE2 gene—is enriched and associated with better lung function(the FEV1/FVC ratio) in Tibetans. Collectively, we generated the first high-resolution Tibetan reference genome, and the identified SVs may serve as valuable resources for future evolutionary and medical studies.

关键词： long-read sequencing Tibetan structural variants genetic adaptation reference genome

电动汽车驱动/充电一体化系统及其控制策略综述

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电工技术学报 2023年第22期38卷 5940-5958页

作者：王晓姬王道涵王柄东王秀和山东大学电气工程学院济南250061

传统电动汽车驱动和充电系统相互独立工作,存在充电容量小、质量体积大、成本高等问题。而驱动充电一体化系统通过将电机绕组和逆变器进行合理重构,复用于充电系统,可以实现系统轻量化和高度集成化,进一步提高系统功率密度。该文首先对... 详细信息

传统电动汽车驱动和充电系统相互独立工作,存在充电容量小、质量体积大、成本高等问题。而驱动充电一体化系统通过将电机绕组和逆变器进行合理重构,复用于充电系统,可以实现系统轻量化和高度集成化,进一步提高系统功率密度。该文首先对当前国内外提出的驱动充电一体化拓扑结构进行归纳总结分类,论述各类型一体化拓扑结构的工作原理、优点以及存在的主要问题;然后,针对目前驱动充电一体化系统存在的关键技术问题对电动汽车不同运行模式下的控制策略进行综述;最后,对电动汽车驱动充电一体化系统的发展趋势进行展望。

关键词：驱动充电系统一体化拓扑电动汽车控制策略

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S422型浆纱机退绕张力控制回路的改进

棉纺织技术 2007年第9期35卷 53-55页

作者：王小记王艳陕西风轮纺织股份有限公司浙江纺织服装职业技术学院

为解决异经产品浆纱过程中经纱伸长率不同造成一部分经纱松弛、一部分经纱断头的问题,对S422型浆纱机的经轴退绕张力控制回路进行了改进。通过采取加装调压阀、气压表和两位三通阀等措施,有效控制了异经产品不同经纱经轴的退绕张力,减... 详细信息

为解决异经产品浆纱过程中经纱伸长率不同造成一部分经纱松弛、一部分经纱断头的问题,对S422型浆纱机的经轴退绕张力控制回路进行了改进。通过采取加装调压阀、气压表和两位三通阀等措施,有效控制了异经产品不同经纱经轴的退绕张力,减少了经纱断头,提高了织机效率和布面质量。

关键词：浆纱机经轴退绕张力伸长率气压

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交流调磁型永磁同步电机磁通协同调控最大转矩铜耗比控制

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电工技术学报 2024年第12期39卷 3630-3645页

作者：王柄东王道涵王晓姬许广生王秀和山东大学电气工程学院济南250061

电动汽车驱动系统不仅需要拥有宽广的调速范围和恒功率运行区域,还应具备高效率、低损耗的优点,以实现续航的最大化。针对这些技术特点,该文研究了一种调磁性能优越的交流调磁型永磁同步电机(ACFR-PMSM),分别建立其等效磁路模型和稳态... 详细信息

电动汽车驱动系统不仅需要拥有宽广的调速范围和恒功率运行区域,还应具备高效率、低损耗的优点,以实现续航的最大化。针对这些技术特点,该文研究了一种调磁性能优越的交流调磁型永磁同步电机(ACFR-PMSM),分别建立其等效磁路模型和稳态数学模型。并以该电机特殊的径向-轴向结构为基础,研究了其电感特性和转矩输出特性,从理论的角度论证了实现最大转矩铜耗比(MTPCL)控制的可行性。另外,分别采用拉格朗日乘子法和电流选择穷举法,获得了MTPCL控制的径-轴向定子绕组最佳电流选择方案。最后,实现了所提出控制策略的计算机仿真和样机测试,验证了其良好的动态控制性能,详细对比了经典id=0控制和所提出MTPCL控制的实施效果并计算了运行铜耗。

关键词：交流调磁型永磁电机最大转矩铜耗比控制磁通协同调控

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Prioritizing natural-selection signals from the deep-sequencing genomic data suggests multi-variant adaptation in Tibetan highlanders

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National Science Review 2019年第6期6卷 1201-1222页

作者： Lian Deng Chao Zhang Kai Yuan Yang Gao Yuwen Pan Xueling Ge Yaoxi He Yuan Yuan Yan Lu Xiaoxi Zhang Hao Chen Haiyi Lou xiaoji wang Dongsheng Lu Jiaojiao Liu Lei Tian Qidi Feng Asifullah Khan Yajun Yang Zi-Bing Jin Jian Yang Fan Lu Jia Qu Longli Kang Bing Su Shuhua Xu Key Laboratory of Computational Biology CAS-MPG Partner Institute for Computational BiologyShanghai Institute of Nutrition and HealthShanghai Institutes for Biological SciencesUniversity of Chinese Academy of SciencesChinese Academy of Sciences School of Life Science and Technology Shanghai Tech University State Key Laboratory of Genetic Resources and Evolution Kunming Institute of Zoology Chinese Academy of Sciences State Key Laboratory of Genetic Engineering and Ministry of Education (MOE) Key Laboratory of Contemporary Anthropology School of Life Sciences Fudan University The Eye Hospital School of Ophthalmology &Optometry Wenzhou Medical University China National Center for International Research in Regenerative Medicine and Neurogenetics Institute for Molecular Bioscience Queensland Brain Institute The University of Queensland Key Laboratory for Molecular Genetic Mechanisms and Intervention Research on High Altitude Disease of Tibet Autonomous Region School of Medicine Xizang Minzu University Center for Excellence in Animal Evolution and Genetics Chinese Academy of Sciences Collaborative Innovation Center of Genetics and Development Human Phenome Institute Fudan University

Human genetic adaptation to high altitudes(>2500 m) has been extensively studied over the last few years,but few functional adaptive genetic variants have been identified, largely owing to the lack of deep-genome sequencing data available to previous studies. Here, we build a list of putative adaptive variants, including 63 missense, 7 loss-of-function, 1,298 evolutionarily conserved variants and 509 expression quantitative traits loci. Notably, the top signal of selection is located in TMEM247, a transmembrane protein-coding *** Tibetan version of TMEM247 harbors one high-frequency(76.3%) missense variant, rs116983452(c.248 C > T; ***83 Val), with the T allele derived from archaic ancestry and carried by >94% of Tibetans but absent or in low frequencies(<3%) in non-Tibetan populations. The rs116983452-T is strongly and positively correlated with altitude and significantly associated with reduced hemoglobin concentration(p = 5.78 × 10), red blood cell count(p = 5.72 × 10) and hematocrit(p = 2.57 × 10). In particular, TMEM247-rs116983452 shows greater effect size and better predicts the phenotypic outcome than any EPAS1 variants in association with adaptive traits in Tibetans. Modeling the interaction between TMEM247-rs116983452 and EPAS1 variants indicates weak but statistically significant epistatic *** results support that multiple variants may jointly deliver the fitness of the Tibetans on the plateau,where a complex model is needed to elucidate the adaptive evolution mechanism.

关键词： Tibetan adaptive genetic variant high-altitude adaptation next-generation sequencing(NGS) archaic ancestry expression quantitative traits loci(eQTL) tissue-specific expression hemoglobin concentration hypoxia