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Establishment of a humanized mouse model using steady-state peripheral blood-derived hematopoietic stem and progenitor cells facilitates screening of cancer-targeted T-cell repertoires

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Cancer Innovation 2024年第3期3卷 1-21页

作者： Yulin Xu Wei Shan Qian Luo Meng Zhang Dawei Huo Yijin chen Honghu Li Yishan Ye xiaohong Yu Yi Luo He Huang Bone Marrow Transplantation Center The First Affiliated HospitalZhejiang University School of MedicineHangzhouChina Liangzhu Laboratory Zhejiang UniversityHangzhouChina Institute of Hematology Zhejiang UniversityHangzhouChina Zhejiang Province Engineering Research Center for Stem Cell and Immunity Therapy HangzhouChina School of Medicine Zhejiang UniversityHangzhouChina

Background:Cancer-targeted T-cell receptor T(TCR-T)cells hold promise in treating cancers such as hematological malignancies and breast cancers.However,approaches to obtain cancer-reactive TCR-T cells have been unsuccessful.Methods:Here,we developed a novel strategy to screen for cancer-targeted TCR-T cells using a special humanized mouse model with person-specific immune fingerprints.Rare steady-state circulating hematopoietic stem and progenitor cells were expanded via three-dimensional culture of steady-state peripheral blood mononuclear cells,and then the expanded cells were applied to establish humanized mice.The human immune system was evaluated according to the kinetics of dendritic cells,monocytes,T-cell subsets,and cytokines.To fully stimulate the immune response and to obtain B-cell precursor NAML-6-and triple-negative breast cancer MDA-MB-231-targeted TCR-T cells,we used the inactivated cells above to treat humanized mice twice a day every 7 days.Then,human T cells were processed for TCRβ-chain(TRB)sequencing analysis.After the repertoires had been constructed,features such as the fraction,diversity,and immune signature were investigated.Results:The results demonstrated an increase in diversity and clonality of T cells after treatment.The preferential usage and features of TRBV,TRBJ,and the V–J combination were also changed.The stress also induced highly clonal Science and Technology,Grant/Award Number:2021C03010;Zhejiang Provincial Natural Science Foundation of China,Grant/Award Numbers:LTGY24H080003,LY21H080004 expansion.Tumor burden and survival analysis demonstrated that stress induction could significantly inhibit the growth of subsequently transfused live tumor cells and prolong the survival of the humanized mice.Conclusions:We constructed a personalized humanized mouse model to screen cancer-targeted TCR-T pools.Our platform provides an effective source of cancer-targeted TCR-T cells and allows for the design of patient-specific engineered T cells.It therefore has the potential to greatly benefi

关键词： cancer-targeted T-cell receptor T(TCR-T)cells circulating hematopoietic stem and progenitor cells(HSPCs) humanized mouse model steady-state peripheral blood T-cell receptorβ-chain(TRB) three-dimensional culture

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αExtractor: a system for automatic extraction of chemical information from biomedical literature

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Science China(Life Sciences) 2024年第3期67卷 618-621页

作者： Jiacheng Xiong xiaohong Liu Zhaojun Li Hongzhong Xiao Guangchao Wang Zhenjiang Niu Chaoyuan Fei Feisheng Zhong Gang Wang Wei Zhang Zunyun Fu Zhiguo Liu Kaixian chen Hualiang Jiang Mingyue Zheng Drug Discovery and Design Center State Key Laboratory of Drug ResearchShanghai Institute of Materia MedicaChinese Academy of SciencesShanghai 201203China University of Chinese Academy of Sciences Beijing 100049China AI Department Suzhou Alphama Biotechnology Co.Ltd.Suzhou 215125China College of Computer and Information Engineering Dezhou UniversityDezhou 253023China

Dear Editor,Great progress has been made using artificial intelligence(AI) techniques in learning knowledge from biomedical databases in recent years, revolutionizing the study of many fields, such as protein structure prediction and protein design(Madani et al., 2023). However, there is massive biomedical knowledge not curated in the form of structured data but hidden in primary scientific literature.

关键词： has such knowledge

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An evaluation model of water-saving reconstruction projects based on resource value flows

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Frontiers of Engineering Management 2022年第2期9卷 257-267页

作者： Runwen JIANG Xiaohong Chen Lingchu ZHAO Zhifang ZHOU Tao ZHANG Central South University Changsha 410083China Collaborative Innovation Center of Resource-conserving&Environment-friendly Society and Ecological Civilization Central South UniversityChangsha 410083China Mobile E-business 2011 Collaborative Innovation Center of Hunan Province Hunan University of Technology and BusinessChangsha 410205China Loughborough University London London E203BSUK

Due to uncertainties in water supply,there is growing demand for water resource management in enterprises.In this study,we evaluated the effects of companies’water-saving reconstruction projects.We used Hina Advanced Materials Company as a case to construct an investment decision model to(1)calculate the internal and external costs of water resources based on circular economic value analysis theory,and(2)locate the level of water resources circulation.We adopted gray situation decision analysis to identify the typical problems that occur in water resource utilization.Moreover,we demonstrated optimization plans for different potential improvements,thereby providing guidance and references for water resource cost management and the comprehensive optimization of environmental benefits.We concluded that the circulation economic value analysis model can effectively display the flow and amount of value derived from water resource flows,thereby providing guidance and suggestions for optimizing water resource flows.

关键词： value flow analysis ternary materials enterprises grey situation decision analysis water resources

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iTRAQ-based proteomics reveals the mechanism of action of Yinlai decoction in treating pneumonia in mice consuming a high-calorie diet

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Journal of Traditional Chinese Medical Sciences 2024年第1期11卷 21-32页

作者： Qianqian Li Tiegang Liu chen Bai Xueyan Ma Hui Liu Zi’an Zheng Yuxiang Wan He Yu Yuling Ma xiaohong Gu School of Traditional Chinese Medicine Beijing University of Chinese MedicineBeijing 102488China Institute of Chinese Medicine Epidemic Disease Beijing University of Chinese MedicineBeijing 102488China School of Traditional Chinese Medicine Guizhou University of Traditional Chinese MedicineGuiyang 550025China Department of Acupuncture and Mini-invasive Oncology Beijing University of Chinese Medicine Third Affiliated HospitalBeijing 100029China OCMRC Center University of OxfordOxford OX13PTUnited Kingdom

Objective:To uncover the underlying mechanisms of action of the Yinlai decoction on high-calorie dietinduced pneumonia through proteomics analysis.Methods:Based on the Gene Expression Omnibus(GEO)database,lung tissue samples from normal and high-fat diet(HFD)fed mice in the GSE16377 dataset were selected as test cohorts to identify differentially expressed genes and conduct bioinformatics analyses.In the animal experiments,mice were randomly divided into the control(N),high-calorie diet pneumonia(M),and Yinlai decoction treatment(Y)groups.Mice in the M group received high-calorie feed and a 0.5 mg/mL lipopolysaccharide solution spray for 30 min for 3 d.The mice in the Y group were intragastrically administered 2 mL/10 g Yinlai decoction twice daily for 3 d.Pathological evaluation of the lung tissue was performed.Differentially expressed proteins(DEPs)in the lung tissue were identified using quantitative proteomics and bioinformatics analyses.The drug-target relationships between Yinlai decoction and core DEPs in the lung tissue were verified using AutoDock Vina and Molecular Graphics Laboratory(MGL)Tools.DEPs were verified by western blot.Results:GEO data mining showed that an HFD altered oxidative phosphorylation in mouse lung tissue.The Yinlai decoction alleviated pathological damage to lung tissue and pneumonia in mice that were fed a high-calorie diet.A total of 47 DEPs were identified between the Y and M groups.Enrichment analysis revealed their association with energy metabolism pathways such as the tricarboxylic acid cycle(TCA)and oxidative phosphorylation.The protein-protein interaction network revealed that Atp5a1,Pdha1,and Sdha were the target proteins mediating the therapeutic effects of Yinlai decoction.Molecular docking results suggested that the mechanism of the therapeutic effect of Yinlai decoction involves the binding of brassinolide,praeruptorin B,chrysoeriol,and other components in Yinlai decoction to Atp5a1.Conclusion:The Yinlai decoction alleviated lung tissue damage and pneumonia in mice tha

关键词： Yinlai decoction High-calorie diet Pneumonia iTRAQ proteomics Energy metabolism

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Catalytic mechanism of in-situ Ni/C co-incorporation for hydrogen absorption of Mg

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Journal of Magnesium and Alloys 2023年第5期11卷 1815-1824页

作者： Bogu Liu Bao Zhang Haixiang Huang Xiaohong Chen Yujie Lv Zhongyu Li Jianguang Yuan Ying Wu Central Iron&Steel Research Institute Advanced Technology&Materials Co.LtdNo.76 XueyuannanluHaidian DistrictBeijing 100081China Advanced Materials Research Institute North China Electric Power UniversityNo.2 Beinonglu Changping DistrictBeijing 102206China

Ni and carbon materials exhibit remarkable catalysis for the hydriding reaction of Mg.But the underlying mechanism of Ni/C hybrid catalysis is still unclear.In this work,density functional theory(DFT)calculation is applied to investigate the effect of Ni/C co-incorporation on the hydriding reaction of Mg crystal.The morphology and crystal structure of the Ni/C co-incorporated Mg sample show that the coincorporated structure is credible.The transition state searching calculation suggests that both the incorporations of Ni and C are beneficial for the H_(2) dissociation.But Ni atom has a dramatic improvement for H_(2) dissociation and makes the H diffusion become limiting step of the hyriding reaction.The Ni dz_(2)orbit and H s orbit accept the electrons and combine together compactly,while the Ni d_(xy) orbit is half-occupied.The catalytic effect of Ni on H_(2) dissociation can be ascribed to the bridging effect of Ni d_(xy) orbit.The incorporation of C can weaken the over-strong interaction between Ni and H which hindered the H diffusion on Mg(0001).The Ni/C co-incorporated Mg(0001)shows the best performance during hyriding reaction compared with the clean and single incorporated Mg(0001).

关键词： Magnesium Ni/C co-incorporation Density functional theory Catalytic effect Hydriding reaction

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Joint probability analysis of water and sediment and predicting sediment load based on copula function

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International Journal of Sediment Research 2022年第5期37卷 639-652页

作者： Haoyu Jin Xiaohong Chen Ruida Zhong Yingjie Pan Tongtiegang Zhao Zhiyong Liu Xinjun Tu School of Civil Engineering Sun Yat-sen University Guangdong Engineering Technology Research Center of Water Security Regulation and Control for Southern China Sun Yat-sen University Key Laboratory of Water Cycle and Water Security in Southern China of Guangdong High Education Institute Sun Yat-sen University Dongjiang Water Source Project Management Division of Shenzhen

The Jinsha River comprises the upper reaches of the Yangtze River, which is the river section with the highest sediment content. Monitoring of sediment transport in the Jinsha River is done to the guarantee for the normal operation of the Three Gorges Reservoir. In the current study, a copula function was used to do a joint probability analysis of the water and sediment in the Jinsha River Basin(JRB), further a sediment load prediction model based on the copula function also was constructed. The results show that the average annual flow from 2001 to 2018 at the outlet of the Jinsha River(Yibin station) is about60.43 billion m3, and the average annual sediment load is about 58.82 million t. The linear correlation coefficient between annual flow and annual sediment load is 0.28. The best marginal distribution for annual flow and sediment load is Pearson Type Three(PE3) and Generalized Normal(GNO), respectively,and the best fit for the combined distribution of the two variables is the Frank copula function. The synchronous probability of water and sediment occurrence is 0.459, and the asynchronous probability is0.541. Based on the copula prediction model, the sediment load can be effectively simulated, and the correlation coefficient between the simulated sequence and the measured sequence reached 0.93. The current study provides important significance for the analysis of water and sediment in the JRB, which is beneficial to the management of Three Gorges Reservoir sediment discharge in the upstream and downstream.

关键词： Marginal distribution Joint distribution Copula Flow Sediment load Jinsha River Basin (JRB)

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EXTENSION OF THE TOPSIS METHOD BASED ON PROSPECT THEORY AND TRAPEZOIDAL INTUITIONISTIC FUZZY NUMBERS FOR GROUP DECISION MAKING

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Journal of Systems Science and Systems Engineering 2014年第2期23卷 231-247页

作者： Xihua LI Xiaohong Chen School of Business Central South University

Considering the decision maker＇s risk psychological factors and information ambiguity under uncertainty, a novel TOPSIS based on prospect theory （PT） and trapezoidal intuitionistic fuzzy numbers （YrIFNs） for group decision making is investigated, in which the criteria values and the criteria weights take the form of TrIFNs, and weights of decision makers are unknown. Firstly, distance measures for TrIFNs are used to induce value function under trapezoidal intuitionistic fuzzy environment. Secondly, the concepts of distance measures and trapezoidal intuitionistie fuzzy weighted averaging operator are employed to induce the weights of decision makers and thus the decision makers＇ options can be aggregated. Then the PT-based separation measures and relative closeness coefficient are defined and an algorithm for ranking alternatives under trapezoidal intuitionistic fuzzy environment is proposed. Finally, a numerical example further illustrates the practicality and effectiveness of the proposed TOPSIS method.

关键词： TOPSIS trapezoidal intuitionistic fuzzy numbers prospect theory group decision making distance measures

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Artificial simulated saliva,gastric and intestinal digestion and fermentation in vitro by human gut microbiota of intrapolysaccharide from Paecilomyces cicadae TJJ1213

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Food Science and Human Wellness 2023年第2期12卷 622-633页

作者： Juanjuan Tian Xiaomeng Wang Xueliang Zhang Xiaohong Chen Mingsheng Dong Xin Rui Qiuqin Zhang Mei Jiang Wei Li College of Food Science and Technology Nanjing Agricultural UniversityNanjing 210095China

In this study,the in vitro digestion and fermentation of two intra-polysaccharide fractions(IPS1 and IPS2)from Paecilomyces cicadae TJJ1213 were investigated.The constituent monosaccharides of IPS1 and IPS2 were not changed after simulated saliva,gastric and small intestinal digestion.However,they can be hydrolyzed and utilized by gut microbiota,and short-chain fatty acids(SCFAs)level were increased after IPS1 and IPS2 treatments.Furthermore,16 S r RNA sequencing analysis of fermentation samples were performed.Alpha-diversity,beta-diversity and taxonomic composition differences analysis revealed that IPS1 and IPS2 promoted the proliferation of beneficial bacteria and modulated the overall structure of gut microbiota.Taxonomic comparison analysis found that IPS1 increased the relative abundances of beneficial bacteria including Megamonas,Bifidobacterium and Lactobacillus,while IPS2 could increase the abundance of Bacteroides,Parabacteroides and Phascolarctobacterium.In addition,they can also decrease the levels of pathogenic bacteria containing Escherichia-Shigella,Klebsiella and Fusobacterium.These results indicated that IPS from Paecilomyces cicadae TJJ1213 could be used as potential candidates for new functional foods.

关键词： Paecilomyces cicadae TJJ1213 Polysaccharide Fermentation Gut microbiota

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尿液中2种全氟化合物测定的液相色谱串联质谱法

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中华劳动卫生职业病杂志 2024年第3期42卷 202-205页

作者：陈晓红邱巧丽宁波市疾病预防控制中心理化检验所浙江省微量有毒化学物健康风险评估技术研究重点实验室宁波315010

目的建立液相色谱串联质谱法快速测定尿液中全氟辛烷磺酸和全氟辛酸含量的方法。方法于2022年11月, 将尿液样品经酸性甲醇提取, WAX固相萃取柱净化, 甲醇水洗脱后, 采用Waters ACQUITY UPLC BEH C18柱(100 mm×2.1 mm, 1.7 μm), 以1.0 ... 详细信息

目的建立液相色谱串联质谱法快速测定尿液中全氟辛烷磺酸和全氟辛酸含量的方法。方法于2022年11月, 将尿液样品经酸性甲醇提取, WAX固相萃取柱净化, 甲醇水洗脱后, 采用Waters ACQUITY UPLC BEH C18柱(100 mm×2.1 mm, 1.7 μm), 以1.0 mmol/L乙酸铵溶液和甲醇为流动相, 进行梯度洗脱;以电喷雾负离子多反应监测模式进行检测, 内标法定量。结果全氟辛酸和全氟辛烷磺酸在0.5~50.0 μg/L浓度范围内均具有良好的线性关系, 相关系数均>0.999, 方法的检出限为0.017 μg/L, 定量限为0.005 μg/L, 平均加标回收率分别为96.3%和101.8%, 日内精密度分别为3.5%~6.2%和3.1%~7.4%, 日间精密度分别为4.3%~6.8%和4.7%~8.1%。结论液相色谱串联质谱方法灵敏度高、准确度好, 可用于人体尿液中全氟辛烷磺酸和全氟辛酸的测定。

关键词：氟化合物色谱法液相串联质谱法固相萃取全氟辛烷磺酸全氟辛酸尿液

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Research progress on rolling superlubricity in solid lubricants

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Science China Technological Sciences 2024年第7期67卷 1980-1990页

作者： LI PanPan LI HongXuan ZHU DongXiang ZHU YongQi KANG FuYan WANG Wan ZHANG YaXin LIU xiaohong JI Li ZHOU HuiDi chen JianMin State Key Laboratory of Solid Lubrication Lanzhou Institute of Chemical Physics Chinese Academy of Sciences Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of Sciences School of Chemical Engineering Northwest Minzu University

Superlubricity is an ideal lubrication state where friction nearly vanishes between contact interfaces. It has become one of the most important research topics and approaches owing to its significance in reducing energy consumption and preventing device failures. As an efficient and universal lubricating principle capable of achieving superlubricity, rolling lubrication has attracted widespread attention in recent years. In this review, the theoretical concept of rolling lubrication and the experimental research progress of spherical/scroll structures are summarized. The review focuses on the possibility of achieving rolling lubrication using spherical/scroll structures(such as spherical fullerenes, carbon nanotubes, and formed and constructed spherical/scroll structures). The challenges in achieving rolling lubrication are summarized, and the possibility of molecular rolling lubrication,as well as its potential applications in superlubrication, are discussed.

关键词： spherical/scroll structure rolling lubrication superlubricity fullerene

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