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免疫出生错误中的免疫缺陷伴自身炎症现象

中国实用儿科杂志 2024年第6期39卷 462-466页

作者：杜鸿强赵晓东重庆医科大学附属儿童医院风湿免疫科、国家儿童健康与疾病临床医学研究中心、儿童发育疾病研究教育部重点实验室、儿童感染与免疫罕见病重庆市重点实验室重庆400014

免疫出生错误(inborn errors of immunity,IEI)是一类单基因遗传病,由编码免疫分子的基因发生可遗传突变所致,其经典的临床表现是反复感染。单基因自身炎症性疾病(monogenic autoinflammatory diseases,mAID)是IEI中一个相对较新的亚类... 详细信息

免疫出生错误(inborn errors of immunity,IEI)是一类单基因遗传病,由编码免疫分子的基因发生可遗传突变所致,其经典的临床表现是反复感染。单基因自身炎症性疾病(monogenic autoinflammatory diseases,mAID)是IEI中一个相对较新的亚类,其经典表现是非感染性的过度炎症。近年来,越来越多的复杂IEI被发现,部分复杂IEI患者同时出现免疫缺陷和自身炎症表现。文章简要总结和介绍这部分IEI的临床表现,以更新临床同仁对IEI或者mAID的认识,减少漏诊漏治。

关键词：免疫出生错误单基因自身炎症性疾病反复感染过度炎症基因突变

The Actin Cytoskeleton Controls FcγRIIB-Mediated Inhibition of B-Cell signaling

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The Actin Cytoskeleton Controls FcγRIIB-Mediated Inhibition...

p16 gene methylation in colorectal cancers associated with Duke's staging

第十三届全国免疫学学术大会

作者： Zhou shu xiaodong zhao children’s hospital of chongqing medical university

FcgRIIB is a major inhibitory coreceptor of B cell receptor(BCR), and its deficiency leads to B cell autoimmunity. Upon colligated with the BCR by immune complexes(ICs), this coreceptor activates sH2-containing inositol-5 phosphatase(sHIP) and inhibits BCR clustering in lipid rafts. However, the molecular mechanisms underlying FcgRIIB-mediated inhibition of BCR activation remain elusive. This study reveals a critical role of the actin cytoskeleton in FcgRIIB-mediated inhibition. Upon interacting with ICs tethered to lipid bilayers, an actomyosin ring is quickly formed surrounding the B cell membrane region interacting with the lipid bilayer(contact zone). Perturbing the actomyosin ring, by myosin inhibitors or B cell-specific gene knockout of non-muscle myosin IIA(NMIIA) or neuronal Wiskott-Aldrich syndrome protein(N-WAsP), releases B cell spreading, BCR clustering and signaling from the inhibition by FcgRIIB. Using conditional gene knockout mice, we further show that NMIIA is recruited to the B cell contact zone in an sHIP-dependent manner, while N-WAsP-mediated actin remodeling organizes NMIIA and actin into a ring. surprisingly, WAsP-mediated actin dynamics is required for the FcgRIIB-induced activation of sHIP but not the formation of the actomyosin ring. Our results suggest that FcgRIIB-triggered signaling induces unique actin remodeling involving NMII, WAsP, and N-WAsP, which limits B cell morphological changes and BCR lateral mobility and enhances the activation of inhibitory signaling. These data potentially provide a mechanistic explanation for the elevated autoantibody levels caused by WAsP or N-WAsP deficiency.

关键词： Actin Cytoskeleton FcγRIIB B cell signaling

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World Journal of Gastroenterology 2001年第5期7卷 722-725页

作者： Jing Yi~1 Zhi-Wei Wang~1 Hui Cang~1 Yu-Ying Chen~1 Ren zhao~2 Bao-Ming Yu~2 Xue-Ming Tang~1 1 Department of Cell Biology,2 Department of surgery,Ruijin hospital,shanghai second medical university,shanghai 200025,China Department of Cell Biology Shanghai Second Medical University 280 Chongqing South Road Shanghai 200025 China. yijing@***

AIM: To explore the association of methylation of the CpG island in the promotor of the p16 tumor suppressor gene with the clinicopathological characteristics of the colorectal cancers. METHODs: Methylation-specific PCR (MsP) was used to detect p16 methylation of 62 sporadic colorectal cancer specimens. REsULTs: p16 methylation was detected in 42% of the ***'staging was associated with p16 methylation status.p16 methylation occurred more frequently in Dukes'C and D patients (75.9%) than in Dukes'A and B patients (12.1%). CONCLUsION: p16 methylation plays a role in the carcinogenesis of a subset of colorectal cancer, and it might be linked to poor prognosis.

关键词： DNA Methylation Colorectal Neoplasms CpG Islands Female Genes, p16 Humans Male Middle Aged Neoplasm staging Polymerase Chain Reaction Research support, Non-U.s. Gov't

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提高进修生肾脏病理学教学质量的实践和体会

山西医科大学学报（基础医学教育版） 2010年第10期12卷 1006-1008页

作者：黄云剑赵景宏聂凌卫静张璟第三军医大学新桥医院肾脏内科重庆400037

对肾脏疾病作出正确的病理诊断是肾科医师必备技能之一。针对进修生教学的特殊性,就如何提高进修生肾脏病理基础知识、如何开展肾脏病理多媒体教学、如何重视临床与病理的结合、如何开展临床病理讨论等方面进行了教学实践,取得了良好的... 详细信息

对肾脏疾病作出正确的病理诊断是肾科医师必备技能之一。针对进修生教学的特殊性,就如何提高进修生肾脏病理基础知识、如何开展肾脏病理多媒体教学、如何重视临床与病理的结合、如何开展临床病理讨论等方面进行了教学实践,取得了良好的教学效果。

关键词：肾脏病理学教学方法教学质量

Hyperactive PI3Kδ predisposes naive T cells to activation via aerobic glycolysis programs

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Cellular & Molecular Immunology 2021年第7期18卷 1783-1797页

作者： Yanjun Jia Qiuyun Yang Yanping Wang Wenyan Li Xuemei Chen Tao Xu Zhirui Tian Minxuan Feng Liang Zhang Wenjing Tang Na Tian Lina Zhou Wenxia song xiaodong zhao National Clinical Research for Child Health and Disorders Chongqing Key Laboratory of Child Infection and ImmunityMinistry of Education Key Laboratory of Child Development and DisordersChildren’s Hospital of Chongqing Medical UniversityChongqingChina National Clinical Research for Child Health and Disorders Chongqing Key Laboratory of PediatricsMinistry of Education Key Laboratory of Child Development and DisordersChildren’s Hospital of Chongqing Medical UniversityChongqingChina Department of Cell Biology and Molecular Genetics University of MarylandCollege ParkMDUSA

Activated phosphoinositide 3-kinaseδsyndrome(APDs)is an autosomal-dominant combined immunodeficiency disorder resulting from pathogenic gain-of-function(GOF)mutations in the PIK3CD *** with APDs display abnormal T cell ***,the mechanisms by which PIK3CD GOF contributes to this feature remain ***,with a cohort of children with PIK3CD GOF mutations from multiple regions of China and a corresponding CRIsPR/Cas9 gene-edited mouse model,we reported that hyperactive PI3Kδdisrupted TNaive cell homeostasis in the periphery by intrinsically promoting the growth,proliferation,and activation of TNaive *** results showed that PIK3CD GOF resulted in loss of the quiescence-associated gene expression profile in naive T cells and promoted naive T cells to overgrow,hyperproliferate and acquire an activated functional *** PIK3CD GOF T cells exhibited an enhanced glycolytic capacity and reduced mitochondrial respiration in the resting or activated *** glycolysis abrogated the abnormal splenic T cell pool and reversed the overactivated phenotype induced by PIK3CD GOF in vivo and in *** results suggest that enhanced aerobic glycolysis is required for PIK3CD GOF-induced overactivation of naive T cells and provide a potential therapeutic approach for targeting glycolysis to treat patients with APDs as well as other immune disorders.

关键词： Primary immunodeficiency disorders Activated phosphoinositide3-kinaseδsyndrome PIK3CD Naive T cells Aerobic glycolysis

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上海市开展家庭病床服务sWOT分析

中国初级卫生保健 2024年第4期38卷 19-22页

作者：刘凤宇王晓栋王伟青麟岚于慧敏赵新平严非复旦大学公共卫生学院上海200032 上海新起点康复医院上海201106

目的:综合分析上海市家庭病床服务的发展现状并提出针对性建议,为其他地区开展家庭病床服务提供参考和借鉴。方法:采用定性定量相结合的方法收集和分析资料,以sWOT分析为框架,探讨上海市开展家庭病床服务的内部优势与劣势,外部机遇与威... 详细信息

目的:综合分析上海市家庭病床服务的发展现状并提出针对性建议,为其他地区开展家庭病床服务提供参考和借鉴。方法:采用定性定量相结合的方法收集和分析资料,以sWOT分析为框架,探讨上海市开展家庭病床服务的内部优势与劣势,外部机遇与威胁。结果:上海市家庭病床服务的优势在于服务网络完善,质量控制管理完善,患者经济负担小、满意度高;劣势在于缺少诊疗规范,人力资源缺乏,针对医护人员的激励不足,未建立统一的信息化系统;机遇在于居民对服务的需求量大,政府政策支持;威胁在于对家庭病床服务的宣传不足,居民对家庭病床服务的质量要求高。结论:上海市开展家庭病床服务,应增强服务能力,加大人力资源投入,动员社会力量参与家庭病床服务,加快建立统一的信息系统,加大宣传力度,并争取更多的医保政策支持。

关键词：家庭病床 sWOT分析服务需求服务能力

A direct,sensitive and high-throughput genus and species-specific molecular assay for large-scale malaria screening

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Infectious Diseases of Poverty 2022年第2期11卷 11-22页

作者： Yaling zhao Ye zhao Yu sun Lihua Fan Duoquan Wang Heng Wang xiaodong sun Zhi Zheng Institute of Basic medical sciences Chinese Academy of Medical SciencesSchool of Basic MedicinePeking Union Medical CollegeBeijingChina Yunnan Institute of Parasitic Diseases Yunnan Center for Malaria ResearchInstitute of Vector and Pathogen BiologyDali UniversityPuerYunnanChina National Institute of Parasitic Diseases Chinese Center for Disease Control and PreventionWHO Collaborating Center for MalariaSchistosomiasisand FilariasisKey Laboratory of Parasite and Vector BiologyMinistry of HealthShanghaiChina Blood Transfusion Department Peking Union Medical College HospitalChinese Academy of Medical SciencesBeijingChina

Background:Infectious disease diagnostics often requires sensitive molecular assays that identify at both genus and species *** large scale screening,such as malaria screening for elimination,diagnostic assay can be a challenge,as both the throughput and cost of the assay must be *** requirement of nucleic acid extraction hampers the throughput of most molecular ***-amplification of multiple species or multiplex identification either can result in missed diagnosis or are too costly for large-scale screening.A genus-and species-specific diagnostic assay with simplified procedure,high sensitivity and throughput is still *** study aimed to develop a sensitive and high-throughput approach for large-scale infectious disease ***:We developed multi-section Capture and Ligation Probe PCR(mCLIP-PCR)for the direct detection of RNA without extraction and reverse *** tailed sandwich hybridization probes were used to bind at genus-and species-specific sections of the target RNA to cooperatively capture the target onto a 96-well *** enzymatic ligation of the bound probes,a single-stranded DNA formed at each section with distinct tail sequence at the *** were separately PCR-amplified with primers corresponding to tail sequences for genus or species *** applied the method to the active screening ofPlasmodium infections of 4,580 asymptomatic dried blood spot samples collected in malaria endemic areas and compared the results with standard qPCR using linear ***:With multi-section cooperative capture but separate amplification strategy,we accurately identified genusPlasmodium and *** *** without RNA extraction,with favorable sensitivities among the published *** the active screening,our method identified all 53 positive infections including two mixed infections,and *** infections that were missed by standard ***:mCLIP-PCR provide

关键词： Infectious disease Malaria Molecular screening CLIP-PCR Genus species High-throughput

Tumor neoantigens: Novel strategies for application of cancer immunotherapy

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Oncology Research 2023年第4期31卷 437-448页

作者： HANYANG GUAN YUE WU LU LI YABING YANG sHENGHUI QIU ZHAN zhao xiaodong CHU JIAsHUAI HE ZUYANG CHEN YIRAN ZHANG HUI DING JINGHUA PAN YUNLONG PAN Department of General surgery The First Affiliated Hospital of Jinan UniversityGuangzhou510632China Department of Gynecology Women’s Hospital of Nanjing Medical UniversityNanjing Maternity and Child Health Care HospitalNanjing210004China Department of Gastroenterology The First Affiliated Hospital of Jinan UniversityGuangzhou510630China

Neoantigen-targeted immunotherapy is a rapidly advancing field that holds great promise for treating *** recognition of antigens by immune cells is a crucial step in tumor-specific killing,and neoantigens generated by mutations in cancer cells possess high immunogenicity and are selectively expressed in tumor cells,making them an attractive therapeutic ***,neoantigens find utility in various domains,primarily in the realm of neoantigen vaccines such as DC vaccines,nucleic acid vaccines,and synthetic long peptide ***,they hold promise in adoptive cell therapy,encompassing tumor-infiltrating cells,T cell receptors,and chimeric antigen receptors which are expressed by genetically modified T *** this review,we summarized recent progress in the clinical use of tumor vaccines and adoptive cell therapy targeting neoantigens,discussed the potential of neoantigen burden as an immune checkpoint in clinical *** the aid of state-of-the-art sequencing and bioinformatics technologies,together with significant advancements in artificial intelligence,we anticipated that neoantigens will be fully exploited for personalized tumor immunotherapy,from screening to clinical application.

关键词： Immunotherapy Tumor vaccine Adoptive T cell therapy Chimeric antigen receptor

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新生儿经鼻高流量氧疗应用指南(2023)

中国循证医学杂志 2024年第4期24卷 385-404页

作者：黄益赵静滑心恬罗可人史源林振浪唐军封志纯母得志中国医师协会新生儿科医师分会循证专业委员会四川大学华西第二医院新生儿科成都610041 出生缺陷与相关妇儿疾病教育部重点实验室成都610041 重庆医科大学附属儿童医院新生儿科重庆400014 温州医科大学附属第二医院浙江温州325000 中国人民解放军总医院儿科医学部北京100007 中国人民解放军总医院第七医学中心儿科医学部研究所北京100007 出生缺陷防控关键技术国家工程实验室北京100007 儿童器官功能衰竭北京市重点实验室北京100007 不详

经鼻高流量(HFNC)氧疗,作为重要的无创呼吸支持模式之一,因为其舒适、易于启动、鼻损伤发生率低等优点,越来越多地应用于呼吸衰竭的危重新生儿。其使用优势、适应证及风险等也是近年研究的热点。基于现有证据,本指南采用证据推荐分级评... 详细信息

经鼻高流量(HFNC)氧疗,作为重要的无创呼吸支持模式之一,因为其舒适、易于启动、鼻损伤发生率低等优点,越来越多地应用于呼吸衰竭的危重新生儿。其使用优势、适应证及风险等也是近年研究的热点。基于现有证据,本指南采用证据推荐分级评估、制订与评价方法(GRADE),并广泛征求新生儿科医生、呼吸治疗师、护理专家及循证医学等专家意见后制订。本指南针对9个关键问题提出了24条建议,旨在成为临床实践中HFNC氧疗的证据和参考来源,使更多的新生儿及其家庭从HFNC中获益。

关键词：氧气吸入疗法鼻导管高流量指南新生儿