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Comparative characterization of antibody responses induced by Ad5-vectored spike proteins of emerging SARS-CoV-2 VOCs

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Signal Transduction and Targeted Therapy 2022年第8期7卷 3126-3138页

作者： Busen Wang Jinghan Xu Shipo Wu Zhe Zhang Zhenghao Zhao Jun Zhang Ling Fu xiaodong zai Yudong Wang Guanying Zhang ZhengShan Chen Yi Chen Hancong Sun Xiaohong Song Jinlong Zhang Lianhui Zhu Lihua Hou Wei Chen Beijing Institute of Biotechnology No.20 Dongdajie StreetFengtai District100071 BeijingChina

Highly divergent SARS-CoV-2 variants have continuously emerged and spread around the world,and updated vaccines and innovative vaccination strategies are urgently needed to address the global SARS-COV2 ***,we established a series of Ad5-vectored SARS-CoV-2 variant vaccines encoding multiple spike proteins derived from the Alpha,Beta,Gamma,Epsilon,Kappa,Delta and Omicron lineages and analyzed the antibody immune responses induced by single-dose and prime-boost vaccination strategies against emerging SARS-CoV-2 variants of concern(VOCs).Single-dose vaccination with SARS-CoV-2 variant vaccines tended to elicit the optimal self-matched neutralizing effects,and Ad5-B.1.351 produced more broad-spectrum cross-neutralizing antibodies against diverse *** contrast,prime-boost vaccination further strengthened and broadened the neutralizing antibody responses against highly divergent SARS-CoV-2 *** heterologous administration of Ad5-B.1.617.2 and Ad5-B.1.429 to Ad5-WT-primed mice resulted in superior antibody responses against most *** particular,the Omicron spike could only stimulate self-matched neutralizing antibodies with infrequent cross-reactivities to other variants used in single-dose vaccination strategies;moreover,with prime-boost regimens,this vaccine elicited an optimal specific neutralizing antibody response to Omicron,and prompted cross-antibody responses against other VOCs that were very similar to those obtained with Ad5-WT ***,this study delineated the unique characteristics of antibody responses to the SARS-CoV-2 VOC spikes with the single-dose or prime-boost vaccination strategies and provided insight into the vaccine development of next SARS-CoV-2 VOCs.

关键词： neutral optimal matched

RBD206-sc-dimer induced robust cross-neutralization against SARS-CoV-2 and variants of concern

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Signal Transduction and Targeted Therapy 2021年第12期6卷 3382-3385页

作者： Chuge Zhou xiaodong zai Ziqing Zhou Ruihua Li Yue Zhang Yaohui Li Ying Yin Jun Zhang Junjie Xu Wei Chen Beijing Institute of Biotechnology No.20 Dongdajie StreetFengtai District100071 BeijingChina

Dear Editor,To date,the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)has caused more than 223 million confirmed cases of coronavirus disease 2019(COVID-19),including 4.6 million deaths(https://***/).Since 2020,several SARS-CoV-2 variants including Alpha(B.1.1.7),Beta(B.1.351),Gamma(P.1),and Delta(B.1.617.2)with immune evasiveness have emerged and fast *** a global research effort,scientists proposed multiple effective vaccine strategies to respond to the ongoing COVID-19 *** vaccines currently in use or in clinical development target the SARS-CoV-2 Spike(S)glycoprotein,a homotrimer decorates on the viral surface;within it,a distinct receptor-binding domain(RBD,residues 331–524)is responsible for mediating cell entry and interaction with host receptor angiotensin-converting enzyme 2(ACE2).

关键词： vaccine acute respiratory

Broadly neutralizing antibodies against Omicron-included SARS-CoV-2 variants induced by vaccination

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Signal Transduction and Targeted Therapy 2022年第5期7卷 1831-1841页

作者： Xiangyang Chi Yingying Guo Guanying Zhang Hancong Sun Jun Zhang Min Li Zhengshan Chen Jin Han Yuanyuan Zhang Xinghai Zhang Pengfei Fan Zhe Zhang Busen Wang xiaodong zai Xuelian Han Meng Hao Ting Fang Jinghan Xu Shipo Wu Yi Chen Yingying Fang Yunzhu Dong Bingjie Sun Jinlong Zhang Jianmin Li Guangyu Zhao Changming Yu Qiang Zhou Wei Chen Institute of Biotechnology Academy of Military Medical SciencesBeijing 100071China Center for Infectious Disease Research Westlake Laboratory of Life Sciences and BiomedicineKey Laboratory of Structural Biology of Zhejiang ProvinceInstitute of BiologyWestlake Institute for Advanced StudySchool of Life SciencesWestlake UniversityHangzhou 310024 Zhejiang ProvinceChina State Key Laboratory of Pathogen and Biosecurity Institute of Microbiology and EpidemiologyAcademy of Military Medical SciencesBeijing 100071China Wuhan Institute of Virology Center for Biosafety Mega-ScienceChinese Academy of SciencesWuhanHubei 430071China

The SARS-CoV-2 Omicron variant shows substantial resistance to neutralization by infection-and vaccination-induced antibodies,highlighting the demands for research on the continuing discovery of broadly neutralizing antibodies(bnAbs).Here,we developed a panel of bnAbs against Omicron and other variants of concern(VOCs)elicited by vaccination of adenovirus-vectored COVID-19 vaccine(Ad5-nCoV).

关键词： neutral vaccination Omi

Precisemodification of the surface charge of antigen enhances vaccine immunogenicity

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The Innovation 2023年第4期4卷 83-90页

作者： xiaodong zai Zhiling Zhang Chuge Zhou Fangxin Zhao Yue Zhang Xiaolin Wang Ruihua Li Yaohui Li Xiaofan Zhao Shuyi Wang Yilong Yang Ying Yin Jun Zhang Junjie Xu Wei Chen Institute of Biotechnology Academy of Military Medical SciencesBeijing 100071China College of pharmacy Nanjing University of Chinese MedicineNanjing 210023China School of Medicine Zhejiang UniversityHangzhou 310058China

Aluminum(alum)adjuvant is the most extensively used protein subunit vaccine adjuvant,and its effectiveness and safety have been widely *** surface charge of the antigen determines its electrostatic adsorption to alum adjuvant,which directly affects the immune efficacy of the protein *** our study,we precisely modified its surface charge by inserting charged amino acids into the flexible region of the SARS-CoV-2 receptorbinding domain(RBD),achieving electrostatic adsorption and a site-specific anchor between the immunogen and alum adjuvant.

关键词： charge electrostatic adsorption

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考虑变形影响的挡土结构受力分析方法及应用

工业建筑 2007年第7期37卷 53-57页

作者：宋林辉梅国雄宰金珉崔晓东南京工业大学土木工程学院南京210009

作用在挡土结构上的土压力会随着土体出现的位移量而发生变化。据此,提出考虑变形影响的挡土结构受力分析方法,如果土体的位移量在极限变形范围内,采用考虑位移的土压力计算公式;如果土体的位移量超出了极限状态,则采用考虑滑动面的土... 详细信息

作用在挡土结构上的土压力会随着土体出现的位移量而发生变化。据此,提出考虑变形影响的挡土结构受力分析方法,如果土体的位移量在极限变形范围内,采用考虑位移的土压力计算公式;如果土体的位移量超出了极限状态,则采用考虑滑动面的土压力计算公式,并将该方法的计算结果与前人的离心模型试验实测结果以及数值模拟结果进行对比分析,验证该分析方法的合理性,最后应用到实际工程,为工程的合理施工提供了正确指导。

关键词：挡土结构土压力位移滑动面极限状态

激光近净成形Inconel 718高温合金的力学性能与耐腐蚀性能

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粉末冶金材料科学与工程 2017年第5期22卷 680-686页

作者：宰雄飞陈仕奇吴宏任俊业刘咏兰小东李清湘周少强刘银中南大学粉末冶金国家重点实验室长沙410083 深圳中金岭南科技有限公司深圳518122

以气雾化Inconel 718高温合金粉末为原料,采用激光近净成形增材制造技术制备用于酸性油田密封件与管道的Inconel 718合金,然后采用2种不同工艺进行热处理。通过扫描电镜与透射电镜观察、拉伸试验以及电化学腐蚀试验,对该合金的组织、力... 详细信息

以气雾化Inconel 718高温合金粉末为原料,采用激光近净成形增材制造技术制备用于酸性油田密封件与管道的Inconel 718合金,然后采用2种不同工艺进行热处理。通过扫描电镜与透射电镜观察、拉伸试验以及电化学腐蚀试验,对该合金的组织、力学性能和耐腐蚀性能进行分析和研究。结果表明:激光成形沉积态Inconel 718合金的主要缺陷为空心颗粒和氧化物颗粒,合金具有优异的抗腐蚀性能,但力学性能较差,抗拉强度为866.9 MPa。经过热处理后,由于γ″和γ′复合强化相的析出,合金的抗拉强度明显提高,伸长率降低。其中经均匀化+固溶+双时效处理的合金具有更优异的力学性能,抗拉强度达1 211.9 MPa,满足油田密封件管道的性能要求,且腐蚀性能优于目前用于酸性油田的轧制态Inconel 718高温合金。

关键词：激光近净成形 Inconel718 力学性能微观组织抗腐蚀性能

独立风/光/储混合微电网多目标电源容量优化配置

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智能电网 2016年第8期4卷 802-810页

作者：高峰闫涛唐巍张筱慧宰红斌乔晓东中国农业大学信息与电气工程学院北京市海淀区100083 国网山西省电力公司晋城供电公司山西省晋城市048000

海岛和偏远地区远离大电网,但可再生能源丰富,采用含风力、光伏等可再生能源的独立微电网供电是解决其电力紧缺的重要手段。为了充分利用可再生能源及更好地满足海岛和偏远地区电力用户需求,提出独立风/光/储微电网电源容量配置的多目... 详细信息

海岛和偏远地区远离大电网,但可再生能源丰富,采用含风力、光伏等可再生能源的独立微电网供电是解决其电力紧缺的重要手段。为了充分利用可再生能源及更好地满足海岛和偏远地区电力用户需求,提出独立风/光/储微电网电源容量配置的多目标优化模型,该模型以电源总成本、负荷缺电率、电能浪费率、风/光输出功率波动率4个指标最小为目标函数,并采用具有自适应交叉和变异操作的改进遗传算法进行求解。对某海岛独立微电网,考虑了单峰和多峰2种日负荷特性校验所提出模型和方法的有效性。仿真结果表明:采用该方法可获得多个可行优化方案,便于决策者根据实际规划目标需要进行选择;当负荷缺电率降低时,电源总成本、能量浪费倍率和风/光输出功率波动率均增大;在负荷缺电率相同的情况下,用该方法获得的方案在电能浪费率和风/光输出功率波动率方面均优于HOMER软件配置方案。

关键词：微电网风光互补容量优化配置供电可靠性独立运行

“文艺独立”与“文以载道”:现当代中国文艺观之三变

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海峡人文学刊 2021年第1期1卷 43-50,156页

作者：邹晓东山东大学儒家文明省部共建协同创新中心、《文史哲》编辑部山东济南250100

在过去一百余年间,中国的主流文艺观发生了三次重大变迁,分别是:从传统儒家式文以载道文艺观,到以新式文以载道+文艺独立情结为特征的新文化运动文艺观;从"资产阶级性质"的新文化运动文艺观,到以阶级论与无产阶级革命精神为本位的思想... 详细信息

在过去一百余年间,中国的主流文艺观发生了三次重大变迁,分别是:从传统儒家式文以载道文艺观,到以新式文以载道+文艺独立情结为特征的新文化运动文艺观;从"资产阶级性质"的新文化运动文艺观,到以阶级论与无产阶级革命精神为本位的思想改造式文以载道文艺观;从教条化的阶级斗争文艺观,到在改革开放与中国和平发展双重语境下,以马克思主义、西方舶来思潮、本土文艺传统相互角力与融通为特征的,既要求思想解放又追求肯定性建构的新时期与新时代文艺观。文以载道(一元论)与文艺独立(二元论或多元论)之间的对立与协调问题,始终贯穿这三变,从而构成现当代中国文艺观演变的基本线索。在文以载道形式下,如何容纳并发挥同文艺独立倾向相伴随的追求创造的冲动,理应成为当下中国文艺观与文艺生态建设所必须着重思考并切实处理好的基本问题。

关键词：文艺观文以载道文艺独立新文化运动思想改造改革开放与新时代政治底线设置创造性工作空间