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Effects of D2 receptor antagonist haloperidol on hippocampal neuronal apoptosis in a rat model of temporal epilepsy

Neural Regeneration Research 2008年第10期3卷 1060-1064页

作者： Songqing Wang Aihua Zhang Haitang Chen Qianghua He Peizeng Xie Yiquan Ke xiaodan jiang Department of Neurosurgery the 422 Hospital of Chinese PLA Zhanjiang 524005 Guangdong Province China Department of Neurosurgery Zhujiang Hospital Southem Medical University Guangzhou 510282 Guangdong Province China

BACKGROUND： Dopamine receptors are divided into D1 and D2 subgroups. It has been reported that D2 receptors resist neural toxicity induced by excitatory amino acids and muscarine, and also alleviate epilepsy attacks following pilocarpine treatment. However, it has not yet been established whether D2 receptors regulate temporal epilepsy. OBJECTIVE： To observe the effects of the D2 antagonist haloperidol on hippocampal neuronal apoptosis and electrical brain activity in a rat model of kainic acid-induced temporal epilepsy. DESIGN, TIME AND SETTING： Randomized grouping and histopathological study were performed at the Neurology Medicine Institute of Zhujiang Hospital, Southern Medical University from August to December 2004. MATERIALS： Twenty-five adult, male, Sprague Dawley rats were selected for the present study. Kainic acid （Sigma, USA） was injected into the right lateral ventricle to establish models of temporal epilepsy. A PowerLah multiplying channel eleetrophysiolograph was provided by AD Instruments, Australia. METHODS： The rats were randomly divided into 5 groups （n = 5）： control, model, haloperidol hippocampus, haloperidol striatum, and haloperidol substantia nigra. Temporal epilepsy was established in all rats except the control group. Haloperidol was slowly injected into the hippocampus, striatum and substantia nigra, respectively, in three different injection groups. Normal saline was injected into the right lateral ventricle of the control rats. MAIN OUTCOME MEASURES： Hippocampal apoptosis was observed on the day 3 of treatment using TUNEL staining. Changes in electroencephalogram at 0, 0.5, 2, 6, and 12 hours following treatment onset were observed using a PowerLab multiplying channel electrophysiolograph. Animal behaviors were classified according to the Racine criteria. RESULTS： Twenty-five rats were included in the final analysis. Seizures did not occur in the control group. In the model group, 10 minutes after kainic acid injection to the lateral ce

关键词： dopamine receptors kainic acid temporal epilepsy

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A Microfluidic System with Active Mixing for Improved Real-Time Isothermal Amplification

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Journal of Beijing Institute of Technology 2022年第3期31卷 275-284页

作者： Dianlong Yang xiaodan jiang Yijie Zhou Xiaobin Dong Luyao Liu Lulu Zhang Xianbo Qiu College of Information Science and Technology Beijing University of Chemical TechnologyBeijing 100029China

To improve the performance of real-time recombinase polymerase amplification(RPA),a microfluidic system with active mixing is developed to optimize the reaction *** of adopting a single typical reaction chamber,a specific reactor including a relatively large chamber in center with two adjacent zig-zag channels at two sides is integrated into the microfluidic *** mixing is achieved by driving the viscous reagent between the chamber and the channel back and forth periodically with an outside compact peristaltic *** avoid reagent evapora-tion,one end of the reactor is sealed with paraffin oil.A hand-held companion device is developed to facilitate real-time RPA amplification within 20 *** whole area of the reactor is heated with a resistance heater to provide uniform reaction *** achieve real-time monitoring,a compact fluorescence detection module is integrated into the hand-held device.A smartphone with custom application software is adopted to control the hand-held device and display the real-time fluorescence *** performances of two cases with and without active on-chip mixing are compared between each other by detecting African swine fever *** has been demonstrated that,with active on-chip mixing,the amplification efficiency and detection sensitivity can be signifi-cantly improved.

关键词： recombinase polymerase amplification(RPA) microfluidic chip active mixing optical detection smartphone

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Synaptic plasticity effects on FeCl_2-induced post-traumatic epilepsy onset in the rat

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Neural Regeneration Research 2010年第18期5卷 1373-1377页

作者： Yuanxiang Lin Feng Wang Ruxiang Xu xiaodan jiang Dezhi Kang Yiquan Ke Mouxuan Du Lishuang Xu Department of Neurosurgery First Affiliated Hospital Fujian Medical University Fuzhou 350005 Fujian Province China Department of Neurosurgery Zhujiang Hospital Southern Medical University Guangzhou 510282 Guangdong Province China

Studies have confirmed that iron induces epilepsy onset, and iron ion-induced epilepsy in anima models closely resembles the clinical situation. Models of post-traumatic epilepsy （PTE） were established by intracortical injection of FeCl2 using stereotactic techniques. Electron microscopy revealed neuronal degeneration, with shrinkage of the neuronal soma, hyperplasia of rough endoplasmic reticulum, ribosomal detachment from the endoplasmic reticulum, and vacuolar degeneration of glial cells in the right frontal lobe of FeCl2-induced PTE rats. With prolonged time injuries became more severe and neuronal apoptosis was observed. Synapses in the hippocampal neuropil significantly increased （primarily type I/excitatory synapses） at day 14 following injury. Type II synapses （inhibitory synapse） were observed in the rat hippocampus at day 30. Cortical neuronal degeneration, apoptosis, glial cell proliferation, and ultrastructural hippocampal changes, in particular changes in type of neuronal synapse, play an important role in PTE onset.

关键词： post-traumatic epilepsy animal model ferrous chloride synapse synaptic plasticity electron microscope electric wire neurodegenerative diseases neural regeneration

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Transient axonal glycoprotein-1 induces apoptosisrelated gene expression without triggering apoptosis in U251 glioma cells

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Neural Regeneration Research 2014年第5期9卷 519-525页

作者： Haigang Chang Shanshan Song Zhongcan Chen Yaxiao Wang Lujun Yang Mouxuan Du Yiquan Ke Ruxiang Xu Baozhe Jin xiaodan jiang Department of Neurosurgery the First Affiliated Hospital of Xinxiang Medical University Neurosurgery Institute Key Laboratory on Brain Function Repair and Regeneration of Guangdong Province Department of Neurosurgery Zhujiang Hospital of Southern Medical University Eight-year Programme the First Clinical Medical College of Southern Medical University Department of Neurosurgery Military General Hospital of Beijing PLA

Previous studies show that transient axonal glycoprotein-1, a ligand of amyloid precursor pro- tein, increases the secretion of amyloid precursor protein intracellular domain and is involved in apoptosis in Alzheimer＇s disease. In this study, we examined the effects of transient axonal glyco- protein-1 on U251 glioma cells. 3-（4,5-Dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide assay showed that transient axonal glycoprotein-1 did not inhibit the proliferation of U251 cells, but promoted cell viability. The terminal deoxynucleotidyl transferase dUTP nick end labeling assay showed that transient axonal glycoprotein-1 did not induce U251 cell apoptosis. Real-time PCR revealed that transient axonal glycoprotein-1 substantially upregulated levels of amyloid precursor protein intracellular C-terminal domain, and p53 and epidermal growth factor recep- tor mRNA expression. Thus, transient axonal glycoprotein-1 increased apoptosis-related gene expression in U251 cells without inducing apoptosis. Instead, transient axonal glycoprotein-1 promoted the proliferation of these glioma cells.

关键词： nerve regeneration brain injury glioma cells transient axonal glycoprotein-1 APP in- tracellular domain p53 epidermal growth factor receptor NSFC grant neural regeneration

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Antitumor Effects of the Fibroblasts Transfected TNF-α Gene and its Mutants

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2002年第2期22卷 92-95页

作者： LI Qingfen LI Li LI Zhuoya GONG Feili FENG Wei jiang xiaodan XIONG Ping Department of Immunology Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030

To compare the anti-tumor effects of transmembrane TNF-α(TM-TNF)and secreted TNF-α(S-TNF)in vivo,mouse fibroblasts NIH3T3 were transfected separately with three types of retrovirus containing wild type TNF-α(Wt-TNF),TM-TNF mutant(TM-TNFm),S-TNF mutant(S-TNFm).Southern blot,RT-PCR,FACS and bioassay were used to investigate TNF-αgene integration,expression and its biological *** was found that both fixed cells and supernatant of NIH3T3/Wt-TNF,the fixed cells of NIH3T3/TM-TNFm and the supernatant of NIH3T3/S-TNFm could express high level of TNF-αor its mutants and effectively kill H22 in *** transfected NIH3T3 were separately injected into the mice at the sites of H22 tumor cell inoculation according to a ratio of 5∶1 or 1∶1(effector/target cells,E/T)after the third day of H22 challenge,*** the E/T=5∶1,the NIH3T3/TM-TNFm induced the highest tumor regression,while NIH3T3/S-TNFm exerted the strongest tumor depressing effect at the E/T=1∶1 in *** obvious side effects were noted throughout the course of *** results suggest that both TM-TNF and S-TNF could cause tumor *** anti-tumor effect of TM-TNF would be more powerful and safe than that of S-TNF at the proper E/T ratio.

关键词： tumor gene therapy transmembrane TNF-α retroviral vector

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Antidepressant-Like Effect of Tetramethylpyrazine in Mice and Rats

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Neuroscience & Medicine 2011年第2期2卷 142-148页

作者： Lijian Yu xiaodan jiang Mingneng Liao Rundi Ma Tingxi Yu Key Laboratory of Marine Materia Medica Guangdong Ocean University

The aim of this study was to investigate the potential antidepressive-like effect of tetramethylpyrazine (TMP), one of available blood-activating and stasis-eliminating components from traditional Chinese medicines, and its mechanism of the antidepressant-like action. Forced-swimming, tail-suspension, reserpine-induced hypothermia, akinesia and ptosis, 5-hydroxytryptophan (5-HTP)-induced head-twitch, and potentiation of noradrenaline (NE) toxicity tests, were per-formed to assess the potential antidepressant-like activity of TMP and to study the mechanism by which TMP exerts the antidepressant-like action. Intragastric (ig) administration of TMP markedly reduced the duration of immobility during forced-swimming tests and tail-supension test in rats and mice. TMP partialy reversed reserpine-induced hypothermia, ptosis and akinesia, and potentiated NE toxicity in mice, and these are similar to those of clomipramine;however, TMP did not potentiate 5-HTP-induced head-twitch response (HTR) in mice, and this is different from that of fluoxetine (FLU). The present data provide evidences that TMP possesses potent antidepressant-like activity, and it might be an adrenergic component of pharmacological activity, and its mechanism of antidepressant-like action is similar to that of clomipramine, and different from that of FLU.

关键词： Tetramethylpyrazine (TMP) Antidepressive-Like Activity Mice Rats Monoaminergic Neurotransmitter

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Non-coding RNAs in acute ischemic stroke:from brain to periphery

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Neural Regeneration Research 2025年第1期20卷 116-129页

作者： Shuo Li Zhaohan Xu Shiyao Zhang Huiling Sun xiaodan Qin Lin Zhu Teng jiang Junshan Zhou Fuling Yan Qiwen Deng Department of Neurology Nanjing First HospitalNanjing Medical UniversityNanjingJiangsu ProvinceChina Department of Neurology Zhongda HospitalSchool of MedicineSoutheast UniversityNanjingJiangsu ProvinceChina General Clinical Research Center Nanjing First HospitalNanjing Medical UniversityNanjingJiangsu ProvinceChina

Acute ischemic stroke is a clinical emergency and a condition with high morbidity,mortality,and *** predictive,diagnostic,and prognostic biomarkers and effective therapeutic targets for acute ischemic stroke remain *** innovations in high-throughput gene sequencing analysis,many aberrantly expressed non-coding RNAs(ncRNAs)in the brain and peripheral blood after acute ischemic stroke have been found in clinical samples and experimental *** expressed ncRNAs in the post-stroke brain were demonstrated to play vital roles in pathological processes,leading to neuroprotection or deterioration,thus ncRNAs can serve as therapeutic targets in acute ischemic ***,distinctly expressed ncRNAs in the peripheral blood can be used as biomarkers for acute ischemic stroke prediction,diagnosis,and *** particular,ncRNAs in peripheral immune cells were recently shown to be involved in the peripheral and brain immune response after acute ischemic *** this review,we consolidate the latest progress of research into the roles of ncRNAs(microRNAs,long ncRNAs,and circular RNAs)in the pathological processes of acute ischemic stroke–induced brain damage,as well as the potential of these ncRNAs to act as biomarkers for acute ischemic stroke prediction,diagnosis,and *** from this review will provide novel ideas for the clinical application of ncRNAs in acute ischemic stroke.

关键词： acute ischemic stroke apoptosis blood–brain barrier damage circular RNAs excitatory toxicity long non-coding RNAs microRNAs neuroinflammation non-coding RNAs oxidative stress

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Antidepressant-Like Activity of Tetramethylpyrazine Measured by Chronic Experimental Method in Rat Model of Depression

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Pharmacology & Pharmacy 2012年第1期3卷 52-57页

作者： Lijian Yu xiaodan jiang Yongping Zhang Mingneng Liao Rundi Ma Tingxi Yu 不详

The aim of this study was to investigate the potential antidepressive-like effect of tetramethylpyrazine (TMP), one of available blood-activating and stasis-eliminating components from traditional Chinese medicines, in chronic mild stress (CMS)-induced rat model of depression. Male Sprague-Dawley (SD) strain rats were divided into six matched groups (n = 13 or 14 in each group) based on their sucrose consumption: control, CMS, CMS + fluoxetine (FLU), and CMS + TMP groups. The rats except control were housed separately in different rooms, and the rat model of depression was established by exposing to an unpredictable sequence of stressors for 28 days;the rats in CMS + FLU were exposed to CMS and received administration of FLU (2.0 mg/kg/d, ig) for 28 days;the rats in CMS + TMP groups were exposed to CMS and received administration of TMP (10, 20, 40 mg/kg/d, ig), respectively, for 28 days. The rats in control group were given ordinary daily care, and the rats in control and CMS groups received ig administration of normal saline instead of FLU or TMP. The body weight, food intake and fluid consumption were measured, and the behaviors were examined by open field test before and after CMS, and forced-swimming test was performed 1 day after last unpredictable stressor. Chronic administration of TMP partially reversed the effects of CMS on consumption of sucrose solution and locomotion and exploration behavior, and potently shortened the immobility time during forced-swimming test following CMS in rats. The results showed that long-term administration of TMP partially reversed the effects of CMS on the body weight gain, the consumption of sucrose solution, the squares crossin gand rearing in open field test, and the immobility time during forced-swimming test in rats. The present data provide evidences that TMP possesses obvious antidepressant-like activity in CMS-induced rat model of depression.

关键词： Tetramethylpyrazine Antidepressive-Like Activity Chronic Mild Stress Rats

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Biochemical properties of norepinephrine as a kind of neurotransmitter secreted by bone marrow-derived neural stem cells induced and differentiated in vitro

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Neural Regeneration Research 2006年第2期1卷 111-114页

作者： Jianrong Chen xiaodan jiang Ruxiang Xu Peng Jin Yuxi Zou Lianshu Ding Institute of Neuromedicine ofChinese PLA Zhujiang Hospi-tal Southern Medical UniversityGuangzhou 510282 Guangdong Province China

BACKGROUND： It has been proved by many experimental studies from the aspects of morphology and immunocytochemistry in recent years that bone marrow stromal cells （BMSCs） can in vitro induce and differentiate into the cells possessing the properties of nerve cells. But the functions of BMSCs-derived neural stem cells（NSCs） and the differentiated neuron-like cells are still unclear. OBJECTIVE： To observe whether bone marrow-derived NSCs can secrete norepinephrine （NE） under the condition of in vitro culture, induce and differentiation, and analyze the biochemical properties of BMSCs-derived NSCs. DESIGN： A non-randomized and controlled experimental observation SETTING ： Institute of Neuromedicine of Chinese PLA, Zhujiang Hospital, Southern Medical University MATERIALS： This experiment was carried out in the Institute of Neuromedicine of Chinese PLA, Zhujiang Hospital, Southern Medical University. The bone marrow used in the experiment was collected from 1.5- month-old healthy New Zealand white rabbits. METHODS： This experiment was carried out in the Institute of Neuromedicine of Chinese PLA, Zhujiang Hospital, Southern Medical University. The bone marrow used in the experiment was collected from 1.5 month-old healthy New Zealand white rabbits. BMSCs of rabbits were isolated and performed in vitro culture, induce and differentiation with culture medium of NSCs and differentiation-inducing factor, then identified with immunocytochemical method. Experimental grouping： ①Negative control group： L-02 hepatic cell and RPMI1640 culture medium were used. ② Background culture group： Only culture medium of NSCs as culture solution was added into BMSCs to perform culture, and 0.1 volume fraction of imported fetal bovine serum was supplemented 72 hours later. ③Differentiation inducing factor group： After culture for 72 hours, retinoic acid and glial cell line-derived neurotrophic factors were added in the culture medium of BMSCs and NSCs as corresponding inducing factors. Th

关键词： bone Biochemical properties of norepinephrine as a kind of neurotransmitter secreted by bone marrow-derived neural stem cells induced and differentiated in vitro stem

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糖尿病视网膜病变患病率的流行病学调查：在中国6省13473名糖尿病患者中的横断面研究

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英国医学杂志中文版 2017年第11期20卷 643-649页

作者： Liu, Yan Song, Yifan Tao, Liyuan Qiu, Weiqiang Lv, Huibin jiang, xiaodan Zhang, Mingzhou Li, Xuemin Peking Univ Dept Ophthalmol Hosp 3 Beijing Peoples R China Peking Univ Res Ctr Clin Epidemiol Hosp 3 Beijing Peoples R China

目的本研究旨在探究中国糖尿病患者中糖尿病视网膜病变（DR）和视力威胁性糖尿病视网膜病变（STDR）的患病率和严重程度。研究设计、设置与参与者本研究为横断面流行病学调查，研究对象为中国内地地区的糖尿病患者（N=13473）。研究对... 详细信息

目的本研究旨在探究中国糖尿病患者中糖尿病视网膜病变（DR）和视力威胁性糖尿病视网膜病变（STDR）的患病率和严重程度。研究设计、设置与参与者本研究为横断面流行病学调查，研究对象为中国内地地区的糖尿病患者（N=13473）。研究对象为被内科医师诊断为糖尿病，并转诊到筛查门诊的糖尿病患者（其中住院患者占1／3，城市居民占1／3，农村居民占1／3）。研究对象的每只眼睛应用彩色、免散瞳和非立体眼底照相机拍摄2张眼底照片，并根据英国DR分级指南进行分级。主要结果和测量指标DR与STDR的患病率和严重程度。结果在参与本研究的13473例糖尿病患者中，共发现4591例DR和1769例STDR患者，DR的总体患病率为34．08％[95％可信区间（凹）33．28％～34．88％]，其中STDR的总体患病率为13．13％（95％C112．56％～13．70％）。在所有研究对象中，12780例参与了眼底照片分级（94．86％）。年龄大于65岁的研究对象患DR或STDR的可能性较低（P〈0．001）。性别对是否患病没有显著影响（P〉0．05）。STDR患者与非STDR患者相比，视力损伤更为严重（P〈0．001）。中国北方DR和STDR的患病率高于南方（P〈0．001）。DR的患病率与糖尿病患病时间f比值比（鲫）2．63；95％C12．42～2．86；P〈0．001]以及糖尿病发病年龄（OR 0．38；95％CI为0．35～0．41；P〈0．001）密切相关。结论DR和STDR在中国内地地区的患病率偏高，并且不同地区患病率存在差异。在DR中，非增殖性DR更常见，但STDR更需要及时的诊断和治疗，特别是在经济欠发达地区。本研究强调了中国内地地区DR筛查和治疗的重要性。

关键词：糖尿病视网膜病变医疗卫生行业

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